SIGNIFICANTLY REDUCE
SALMONELLA AND OTHER
PATHOGENS IN YOUR PLANT
1,2
Amplon™ is a uniquely formulated buffered sulfuric acid that can help reduce
pathogens at multiple processing stages. It provides the antimicrobial benefits
of a strong acid, while minimizing organoleptic damage and risk to personnel
and equipment.
The product is brought to you by Zoetis (zo-EH-tis), the global leader in animal
health, now providing you with innovative post-harvest food safety solutions. In
addition to vaccines, medicines and other animal health products, Zoetis now offers
scientifically advanced food safety products to provide farm-to-fork solutions for
meat and poultry production.
EFFECTIVE AND ECONOMICAL
Amplon is approved by the U.S. Department of Agriculture (USDA) for use as an
antimicrobial, acidifier and processing aid in water used in poultry processing. 3
Product benefits include:
»» Made with U.S. Food and Drug Administration (FDA) Generally
Recognized as Safe (GRAS) ingredients4
»» Easy handling at low pH
»» No off-gassing
»» No significant organoleptic damage
»» Simple to install, use and maintain
»» Can be used at all intervention stages
»» Effective against Escherichia coli (E. coli) and Salmonella spp. 3,5
»» More cost-effective than lactic acid
»» Minimally impacted by organic load; can be recycled
»» May be applied as a spray or dip3
WHEN USED AT EVERY INTERVENTION STAGE, AMPLON™ SIGNIFICANTLY (P < 0.05)
REDUCED AEROBIC PLATE COUNT (APC)1
A study 1 was performed at a processing plant to evaluate the effects of Amplon at multiple intervention stages
(Figure 1). Carcasses were gathered at post-pick, post-New York rinse (NYR), pre-online reprocessing (OLR),
post OLR, post-chill and post-finishing chiller. Carcass rinses were sent to an independent lab for analysis.
ANTIMICROBIAL INTERVENTION — FIRST PROCESSING
FIGURE 1. Amplon was applied at
PAA +
CENTROL™
FRESH WATER
evisceration, online reprocessing
(OLR), chillers and post-chillers.
AMPLON
The solution from the OLR and
AMPLON
VIA DIP TANK
MIXER
post-chiller was recycled to the
New York rinse (NYR).
MIXER
MIXER
PICKERS
NYR
REHANG
EVIS
IOBW
POST
CHILL
OLR
SCALDERS
CHILLERS
RECYCLE
RESULTS
At each stage, Amplon was effective at reducing APC, E. coli and Salmonella.
APC BIOMAP
6
5
FIGURE 2. From post-pick to
DAY 2
post-finishing chiller, Amplon
delivered a significant (P < 0.05)
4
LOG
DAY 1
2-log APC reduction.
3
2
1
0
POST-PICK
POST-NYR
PRE-OLR
POST-OLR
POST-CHILL
POST-FINISHING
CHILLER
KEY POINTS
Amplon:
»» Can be used as part of a multiple intervention strategy
»» Produced a significant (P < 0.05) APC reduction when used at every intervention stage
»» Works by modifying pH rather than oxidizing, so can be recycled at other points in the
intervention process
2
AMPLON™ SIGNIFICANTLY REDUCED SALMONELLA WHEN USED AT OLR2
Another study2 was performed at three different plants to determine the efficacy of Amplon at the
online reprocessing stage. Whole bird carcass rinses were collected under normal conditions from visibly
contaminated (VF) and not visibly contaminated (VU) carcasses, and tested for APC, E. coli and the presence
of Salmonella spp.
INTERACTION OF CARCASS CONDITION WITH SAMPLING LOCATION FOR ALL PLANTS
APC
E. COLI
Sampling Location
Carcass
Condition
a,b
FIGURE 3. With OLR intervention,
Sampling Location
Pre-OLR
Post-OLR
TOTAL
Visibly
Contaminated
(VF)
4.03
3.15
3.59a
Not Visibly
Contaminated
(VU)
3.92
3.15
3.53a
TOTAL
3.98a
3.15b
Carcass
Condition
Amplon reduced APC and E. coli
Pre-OLR
Post-OLR
TOTAL
Visibly
Contaminated
(VF)
2.18x
1.43x
1.81
Not Visibly
Contaminated
(VU)
1. 98y
1.43z
1.70
TOTAL
2.08
1.43
on carcasses.
Interaction means with different superscripts differed (P < 0.05).
Main effect means within a total column or row with different superscripts differed (P < 0.05).
x,y,z
RESULTS
APC results demonstrate the plants achieved control and total bacterial load did not increase.
PERCENT SALMONELLA-POSITIVE
PERCENTAGE OF SALMONELLA-POSITIVE CARCASSES PRE- AND POST-OLR AT THREE PROCESSING FACILITIES
a,b
25
24.50a
20
Pre-OLR
FIGURE 4. When Amplon was used
Post-OLR
at OLR, there was a significant
reduction in carcasses testing
15
positive for Salmonella post-OLR:
14.50b
11.43
10
7.61
Of 1,190 samples, 68 tested
a
positive pre-OLR and 34 tested
a
5.71b
5
2.02a
0
1.51 b
1.02a
PLANT 1
positive post-OLR.
PLANT 2
PLANT 3
OVERALL
Means within the same processing locale with different superscripts differed (P < 0.05).
KEY POINTS
Amplon:
»» Significantly decreased the percentage of carcasses testing positive for Salmonella when used between
pre- and post-OLR
»» Helps control APC, E. coli and Salmonella regardless of organic load coming into the system
3
MAKE THE CHANGE TO AN
EFFECTIVE ANTIMICROBIAL
THAT’S EASIER TO HANDLE.
TO LEARN HOW ZOETIS CAN HELP, CONTACT YOUR ZOETIS REPRESENTATIVE
OR VISIT WWW.ZOETISUS.COM/FOODSAFETY.
Data on file. University of Georgia research study, 2012, Zoetis Inc.
Data on file. Study Report No. 10 OLR 0514-N-A, Zoetis Inc.
U.S. Department of Agriculture Food Safety and Inspection Service. FSIS directive 7120.1, revision 18: Safe and suitable ingredients used in the production of meat, poultry and egg
products. Available at: http://www.fsis.usda.gov/wps/wcm/connect/bab10e09-aefa-483b-8be8-809a1f051d4c/7120.1.pdf?MOD=AJPERES. Accessed September 18, 2014.
4
Food and Drug Administration. Agency response letter GRAS notice no. GRN 000408. Available at: http://www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/NoticeInventory/
ucm300519.htm. Accessed September 18, 2014.
5
Data on file, Study Report No. 06-14-70AQO, Zoetis Inc.
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2
3
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