GE Healthcare
QuixStand™ benchtop systems
Operating Instructions
Original Instructions
Table of Contents
Table of Contents
1
Introduction .............................................................................. 5
1.1
1.2
1.3
2
Safety instructions................................................................. 17
2.1
2.2
2.3
2.4
3
7
Operation overview .............................................................................................. 33
Starting the instrument ...................................................................................... 34
Starting the system .............................................................................................. 36
Constant volume diafiltration .......................................................................... 38
Pressurized feed reservoir ................................................................................. 38
High flow pump tubing (size 18) ..................................................................... 39
Autoclaving .............................................................................................................. 39
Procedures after operation .............................................................................. 39
Maintenance ........................................................................... 41
5.1
5.2
5.3
6
Site requirements .................................................................................................. 25
Unpacking ................................................................................................................. 25
Assembly ................................................................................................................... 26
Connections ............................................................................................................. 32
Spare parts and accessories ........................................................................... 32
Operation ................................................................................ 33
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
5
Safety precautions ............................................................................................... 17
Labels .......................................................................................................................... 21
Emergency procedures ...................................................................................... 21
Recycling procedures .......................................................................................... 23
Installation .............................................................................. 25
3.1
3.2
3.3
3.4
3.5
4
Important user information ................................................................................. 6
Regulatory information ......................................................................................... 8
Instrument ................................................................................................................ 10
Inspection ................................................................................................................. 41
User maintenance schedule ............................................................................ 41
Storing the system ................................................................................................ 41
Troubleshooting ..................................................................... 43
Reference information .......................................................... 49
7.1
7.2
Specifications .......................................................................................................... 49
Ordering information ........................................................................................... 50
QuixStand Operating Instructions 28-9646-70 AA
3
Table of Contents
4
QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
1 Introduction
Purpose of the Operating
Instructions
The Operating Instructions provide you with the instructions needed to handle
QuixStand in a safe way. This user guide describes how to assemble and use the
QuixStand bench top system. Topics include a product description, assembly
instructions, operation instructions, and maintenance procedures.
Prerequisites
In order to operate QuixStand safely and according to the intended purpose the
following prerequisites must be met:
•
You should be acquainted with the use of general laboratory equipment and with
handling of biological materials.
•
You must read Chapter 2 Safety instructions‚ on page 17 of these Operating
Instructions.
•
The system should be installed according to the instructions in Chapter 3 of these
Operating Instructions.
In this chapter
This chapter contains important user information and a general description of
QuixStand and its intended use.
QuixStand Operating Instructions 28-9646-70 AA
5
1
1.1
Introduction
Important user information
1.1 Important user information
Read this before using
QuixStand
All users must read the Safety Instructions in Chapter 2 of these Operating Instructions
before installing, using or maintaining the system.
Do not operate QuixStand in any other way than described in the user documentation.
If you do, you may be exposed to hazards that can lead to personal injury and you may
cause damage to the equipment.
Intended use
QuixStand is a bench top system intended for laboratory-scale filtration of biological
solutions using hollow fiber membrane cartridges under the conditions stated in this
manual. If you use the system and cartridges in a manner not specified by GE
Healthcare, you may impair the protection provided by the system and cartridges.
When using any laboratory, pilot-scale, or production cross-flow filtration equipment,
the potential exists for personal injury unless you follow established safety procedures.
When using GE Healthcare products, you should follow OSHA, federal, state, and local
safety regulations for equipment installation and operation.
You should follow your company’s safety regulations and good engineering practices.
You should follow the specific safety instructions provided in this manual and in the
operating guides of any user supplied equipment. Only qualified personnel who are
adequately trained and who understand the operating instructions should install,
operate, maintain, and inspect the QuixStand system.
These Operating Instructions address the needs of scientists, process engineers, and
technicians who operate laboratory-scale cross-flow filtration systems. The level of
information presented in this guide assumes the user possesses basic laboratory and
technical skills, and has the knowledge and documentation to safely operate any user
supplied equipment connected to the QuixStand bench top system. If you need
assistance with the instructions in this guide, contact GE Healthcare for more
information.
Safety notices
These Operating Instructions contain WARNINGS, CAUTIONS and NOTICES concerning
the use of the product, with meanings as defined below.
6
QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
Important user information 1.1
WARNING
WARNING indicates a hazardous situation which, if not avoided, could
result in death or serious injury. It is important not to proceed until all
stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided, could
result in minor or moderate injury. It is important not to proceed until all
stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid damage to
the product or other equipment.
QuixStand Operating Instructions 28-9646-70 AA
7
1
1.2
Introduction
Regulatory information
Notes and tips
Note: A Note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip:
A tip contains useful information that can improve or optimize your procedures.
1.2 Regulatory information
This section lists the directives and standards that are fulfilled by QuixStand.
Manufacturing information
Requirement
Content
Name and address of manufacturer
GE Healthcare Bio-Sciences AB,
Björkgatan 30, SE 751 84 Uppsala
Sweden
Name and ID of notified body
-
Place and date of declaration
Uppsala, Sweden, May 2009
Identity of person authorized to sign DoC
See EC Declaration of Conformity
CE Conformity
Directive
Title
2006/42/EC
Machinery Directive (MD)
2006/95/EC
Low Voltage Directive (LVD)
2004/108/EC
ElectroMagnetic Compatibility (EMC) Directive
International standards
8
Standard
Description
Notes
EN 61010-1,
IEC 61010-1,
CAN/CSA-C22.2 no.
61010-1
Safety requirements for electrical
equipment for measurement, control
and laboratory use
QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
Regulatory information 1.2
Standard
Description
Notes
EN 61326-1
EMC emissions and immunity
requirements for measurement,
control and laboratory use
Harmonized with
2004/108/EC
EN-ISO
12100-1, 12100-2
Safety of machinery – Basic concepts,
general principles for design
Harmonized with
2006/42/EC
EN-ISO
14121-1, 14121-2
Safety of machinery – Principles of
risk assessment
Harmonized with
2006/42/EC
CE marking
The CE marking and the corresponding Declaration of Conformity is valid for the
instrument when it is:
•
used as a stand-alone unit, or
•
connected to other CE-marked instruments, or
•
connected to other products recommended or described in the user
documentation, and
•
used in the same state as it was delivered from GE Healthcare, except for
alterations described in the user documentation or explicitly authorized by GE
Healthcare.
Regulatory compliance of
connected equipment
Any equipment connected to QuixStand should meet the safety requirements of EN
61010-1/IEC61010-1 or relevant harmonized standards. Within the European Union,
connected equipment must be CE-marked.
QuixStand Operating Instructions 28-9646-70 AA
9
1
1.3
Introduction
Instrument
1.3 Instrument
A typical setup for a QuixStand system includes all the components needed for crossflow filtration with hollow fiber cartridges (Figure 1-1).
10
11
1
2
12
13
3
14
4
15
16
17
5
18
6
19
7
8
9
20
Figure 1-1. Typical QuixStand setup.
Table 1-1. Parts in Figure 1-1.
10
Part
Description
1
Back pressure valve
2
Retentate outlet
3
Reservoir cap, gasket and clamp
4
Feed reservoir (1,000 ml shown)
5
Permanent reservoir attachment
6
Lower manifold
7
Pump cover
QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
Instrument 1.3
Part
Description
8
Pump controls
9
Pump inlet
10
Outlet pressure gauge
11
Support rod cap (not visible)
12
Upper manifold assembly
13
Tightening knob (not visible)
14
Upper permeate outlet (Normally used to collect permeate during a
run)
15
Support rod
16
Xampler cartridge (Size 4M shown)
17
Lower permeate outlet (Normally use to drain permeate after a run)
18
Sanitary connection
19
Inlet pressure gauge
20
Pump outlet
The main components of a QuixStand bench top system are:
•
Lower manifold
•
Support rod
•
Reservoirs
•
Upper manifold
•
Peristaltic pump (optional)
1
2
3
Figure 1-2. Close up of pump.
QuixStand Operating Instructions 28-9646-70 AA
11
1
1.3
Introduction
Instrument
Table 1-2. Parts in Figure 1-2.
Part
Description
1
Pump display and keyboard
2
Cover to pump mechanism
3
Bottom part of pump mechanism
The following sections describe these and other key components.
Lower manifold
The lower manifold consists of a polysulfone manifold with a stainless steel top plate.
The polysulfone block has channels and six ports that direct the flow of process fluid.
The six ports use sanitary connectors to connect the reservoir, hollow fiber cartridge,
feed tubing (to and from the pump), pressure gauge, and a drain/sample valve. Four
rubber bumpers mounted on the bottom of the base support the base assembly.
Support rods
The support rod slips into the lower manifold and supports the upper manifold. The
upper manifold slides up and down along the support rod and locks into place at a
height that matches the cartridge in use. Two support rods are included also allowing
you to match the rod to the length of the cartridge. When a QuixStand model includes
a carrying case, the system uses a two-piece rod so that the rod fits in the case. A quick
connect coupling enables you to assemble the rod.
Reservoirs
QuixStand systems include a 400 ml and 1,000 ml reservoirs. The reservoir holds the
process solution, feeds the solution to the recirculation pump, and collects the retentate
stream. The feed reservoir of choice is connected to the sanitary connector located on
the lower manifold. The system includes a reservoir cap, gasket, and clamp. The cap has
two barbed inlet ports that enable you to connect tubing to the cap. The cap’s two ports
allow recirculation of the retentate stream back to the reservoir and allow additional
feed solution or diafiltrate to be introduced into the reservoir during processing.
The cap fits both reservoirs. The sanitary clamp holds the reservoir cap and silicone
gasket in place. Two polysulfone downcomers are included with each reservoir. The
downcomer is associated with the retentate return line to minimize aeration and
foaming of sample in the feed reservoir.
The two reservoirs supplied with the QuixStand system are graduated to indicate the
nominal volume of fluid in the system. The exact volumes will depend on the size of the
cartridge, the lumen diameter of the hollow fibers, and the size and length of the tubing
used. For the most accurate volume measurements, you should measure the feed
volume prior to processing and collect the permeate in a graduated vessel.
12
QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
Instrument 1.3
Hollow fiber cartridges
You order hollow fiber cartridges separately from the QuixStand system. Consult our
cartridge selection guide, Selecting Hollow Fiber Cartridges and Systems, for a list of
cartridges and cartridge specifications such as membrane pore sizes, fiber lumen
diameters, cartridge lengths, and membrane areas.
Valves
QuixStand systems include a precision back pressure control valve (also called a tubing
pinch valve). The back pressure control valve is installed on the retentate line near the
upper manifold.
QuixStand systems use various drain valves. Systems QSM-02S and QSM-02SP include
a non-sanitary, threaded, nylon sampling/drain valve. Systems QSM-03S, QSM-03SP,
QSM-04SA, and QSM-04SAP include a sanitary, autoclavable, stainless steel sampling/
drain valve (Figure 1-3).
Figure 1-3. Non-sanitary nylon drain valve (left) and sanitary, autoclavable stainless steel valve
(right)
Pressure gauges
Each QuixStand system includes two 0 to 2 barg (0 to 30 psig) pressure gauges to
monitor feed and retentate pressures. Non-sanitary QuixStand systems (models QSM02S and QSM-02SP) use gauges with threaded fittings. Sanitary—but not autoclavable—
QuixStand systems (models QSM-03S and QSM-03SP) use stainless steel gauges with
0.75-inch sanitary fittings. Sanitary, autoclavable QuixStand systems (models QSM-
QuixStand Operating Instructions 28-9646-70 AA
13
1
1.3
Introduction
Instrument
04SA and QSM-04SAP) use stainless steel pressure gauges with 1.5 inch sanitary fittings
(Figure 1-4).
Figure 1-4. Pressure gauges with threaded (left), 0.75 inch sanitary (middle), and 1.5 inch sanitary
(right) fittings.
Upper manifold
The upper manifold has channels and ports to direct the flow of process fluid out of the
hollow fiber cartridge and back to the feed reservoir or collection vessel.
The manifold has three ports (sanitary connectors) to allow you to connect a hollow
fiber cartridge, retentate pressure gauge, and retentate line to the manifold.
A knob on the back of the upper manifold enables you to loosen and adjust the position
of the manifold on the support rod. You can match the height of the manifold to the
height of the cartridge you are using.
Peristaltic pump (optional)
Some QuixStand models include a Watson-Marlow peristaltic pump. QuixStand
systems with a P in the model number (QSM- 03SP for example) include a pump. Read
the supplied Watson-Marlow pump user guide before using the system and peristaltic
pump. Submit the warranty registration for the pump directly to the pump
manufacturer using the card provided.
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QuixStand Operating Instructions 28-9646-70 AA
Introduction 1
Instrument 1.3
Accessory kit
QuixStand systems include an accessory kit containing clamps, gaskets, o-rings and
adaptors useful for operating the system (Figure 1-5).
Figure 1-5. QuixStand accessory kit.
QuixStand Operating Instructions 28-9646-70 AA
15
1
1.3
Introduction
Instrument
QuixStand system flow path
The standard flow path for the QuixStand system includes a feed reservoir, peristaltic
feed pump, hollow fiber cartridge, valves and pressure gauges.
2
1
P
3
4
5
Recirculation
Reservoir
8
P
7
6
Figure 1-6. QuixStand bench top system flow diagram.
16
1
Diafiltrate port
2
Backpressure valve
3
Retentate
4
Permeate
5
Hollow fibre cartridge
6
Inlet pressure gauge
7
Sampling/Drain valve
8
Peristaltic recirculation pump
QuixStand Operating Instructions 28-9646-70 AA
Safety instructions 2
Safety precautions 2.1
2 Safety instructions
This chapter describes safety compliance, safety labels, general safety precautions,
emergency procedures, power failure and recycling of QuixStand.
2.1 Safety precautions
Introduction
Before installing, operating or maintaining the system, you must be aware of the
hazards described in the user documentation. Follow the instructions provided to avoid
personal injury or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•
General precautions
•
Flammable liquids
•
Personal protection
•
Installing and moving the instrument
•
Maintenance
QuixStand Operating Instructions 28-9646-70 AA
17
2
2.1
Safety instructions
Safety precautions
General precautions
WARNING
If you overpressurize the system with compressed air during integrity
testing, system components can rupture causing bodily harm. To avoid
the rupture of a system component due to over pressurization with
compressed air, use a pressure regulator to limit the pressure of your
compressed air supply to the maximum operating pressure of your
system.
WARNING
Wear the appropriate personal protection devices when testing the
system.
WARNING
If the system leaks, it can release potentially hazardous process fluids or
cleaning fluids causing bodily harm. To prevent leaks and the release of
potentially hazardous process or cleaning fluids, limit feed pressure to
the maximum operating pressure of your system.
WARNING
Mishandling potentially hazardous process and cleaning/storage
solutions can cause bodily harm through exposure to skin, eyes, and
respiratory system. To safely handle potentially hazardous process and
cleaning/storage solutions, read the MSDS for the solutions you use.
Follow the handling and safety instructions on the MSDSs and use the
personal protection equipment required by your company, local, state,
and federal laws.
Notices
NOTICE
Starting the optional peristaltic pump with the voltage selector set to the
incorrect voltage can result in pump motor damage. To prevent pump
motor damage, ensure the voltage selector switch is set to the proper
voltage (110V/60 Hz or 220V/50 Hz) before starting the pump.
NOTICE
Failure to open the pump head and relieve the pressure on the tubing in
a peristaltic pump when the pump is stored can accelerate tubing wear.
To prevent premature wear, open the pump head and relieve the
pressure on the tubing when the pump is not being used.
18
QuixStand Operating Instructions 28-9646-70 AA
Safety instructions 2
Safety precautions 2.1
NOTICE
Failure to carefully plan the installation of the system can result in
equipment damage or poor performance. To avoid equipment damage,
carefully plan the installation of the system in reference to the effects of
a power failure and utility connections. Read the installation instructions
in this manual.
NOTICE
Hollow fiber cartridges are strong and safe, but mishandling can
damage the membrane fibers or cartridge housing connectors. Follow
guidelines in Operations section to prevent damage to the hollow fibers
and cartridge housing connectors.
Using flammable liquids
WARNING
An adequately dimensioned ventilation system shall be installed when
flammable or noxious substances are used.
Personal protection
WARNING
Wear the appropriate personal protection devices and clothing when
operating and cleaning the system. Basic personal protection devices
include protective clothing and safety glasses. Use additional protective
devices (fume hood, respirator, etc.) if the nature of your product or your
company’s safety regulations require it.
WARNING
Wear protective glasses at all times when in vicinity of instrument.
Installing and moving the
instrument
WARNING
Use tubing clamps on all hose barb connections, particularly
connections to the feed ports for leak-free connections at high pressure.
Failure to do so may lead to unexpected leaks that my lead to bodily
harm.
QuixStand Operating Instructions 28-9646-70 AA
19
2
2.1
Safety instructions
Safety precautions
WARNING
The system must be connected to a grounded mains socket.
WARNING
Do not block the rear panel of the system. The mains power switch must
always be easy to access.
WARNING
Failure to wet the o-rings before you install barbed, hollow-fiber
cartridges into end adaptors can result in deformation and improper
seating/sealing of the o-ring. This can also cause unexpected leaks of
potentially hazardous fluids and result in bodily harm. To prevent leaks
and damage, wet the o-rings with water before each use.
WARNING
Using tubing not designed for peristaltic pumps in the peristaltic pump
can cause the tubing to fail prematurely, exposing the operator to
potentially hazardous process or cleaning fluids. To prevent the
premature or unexpected failure of the tubing, use special, heavy-duty
tubing intended for use in peristaltic pumps.
System operation
WARNING
Ensure that the retentate valve is open before starting the feed pump.
Failure to do so can cause the feed pressure to reach excessively high
levels causing leaks, bodily harm, and damage to the cartridge.
Maintenance
WARNING
Disconnect the power cable, the compressed air supply, and
decompress the system before inspecting or maintaining the system.
Failure to do so can result in bodily harm from the accidental starting of
the pump or unexpected release of pressure.
WARNING
Decontaminate the equipment before decommissioning to ensure the
removal of all hazardous residues.
20
QuixStand Operating Instructions 28-9646-70 AA
Safety instructions 2
Labels 2.2
2.2 Labels
This section describes safety labels and labels concerning hazardous substances that
are attached to the QuixStand.
Labels on the instrument
The QuixStand system label, located on the bottom of the base plate, and the pump
label (if supplied with a pump) on the back of the pump housing, provide key
identification and specification information (Figure 2-1). The CE mark for European
models is located on the back of the base.
Figure 2-1. Typical pump label.
XX-XXXX-XX
Code No:
Serial No
Mfg Year:
QuixStand
XXXXXXXX
XXXXXXX
2009
Made in Sweden GE Healthcare Bio-Sciences AB
751 84 Uppsala Sweden
Figure 2-2. Example of QuixStand marking label.
2.3 Emergency procedures
This section describes how to do an emergency shutdown of the QuixStand system. The
section also describes the result in the event of power failure.
QuixStand Operating Instructions 28-9646-70 AA
21
2
2.3
Safety instructions
Emergency procedures
Emergency procedures
In an emergency situation, do as follows to stop the run:
Step
Action
1
Press the Stopbutton on the pump keypad.
2
Disconnect the electrical power cord from the mains socket outlet.
3
Investigate and remedy the reason for the emergency stop.
Power failure
A power failure will interrupt your process but does not affect the safe operation of the
equipment.
22
QuixStand Operating Instructions 28-9646-70 AA
Safety instructions 2
Recycling procedures 2.4
2.4 Recycling procedures
The equipment shall be decontaminated before decommissioning and all local
regulations shall be followed with regard to scrapping of the equipment.
Disposal, general instructions
When taking QuixStand out of service, the different materials must be separated and
recycled according to national and local environmental regulations.
Recycling of hazardous
substances
QuixStand contains hazardous substances. Detailed information is available from your
local GE Healthcare representative.
Disposal of electrical
components
Waste of electrical and electronic equipment must not be disposed as unsorted
municipal waste and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning the decommissioning of
your equipment.
QuixStand Operating Instructions 28-9646-70 AA
23
2
2.4
24
Safety instructions
Recycling procedures
QuixStand Operating Instructions 28-9646-70 AA
Installation 3
Site requirements 3.1
3 Installation
NOTICE
Fittings and connectors are made of plastic and can crack if clamps are
overtightened. Do not overtighten sanitary clamps when connecting
fittings and components together.
System installation requires choosing a location, assembling the system, and
connecting the pump to an electrical power supply.
3.1 Site requirements
Parameter
Requirement
Electrical power
100-120 VAC (50/60 Hz) or 220-240 VAC (50/60 Hz)
Placement
Stable laboratory bench
Ambient temperature
5ºC to 40ºC (recommended)
Humidity
< 90% RH
Select a location that includes:
•
appropriate drains or collection vessels to collect waste and processed fluids, and
•
electrical power supply.
A commonly used location is on a laboratory bench near a sink.
The system is lightweight and portable and no special lift and carry instructions are
required.
3.2 Unpacking
Your QuixStand system arrives partially assembled. All components are packaged in a
heavy-duty cardboard box, including the optional peristaltic pump. Remove all parts
from the package and set them out on a bench or table.
All QuixStand systems include these assemblies and documents:
•
400-ml and 1,000-ml reservoirs
•
Lower manifold
•
Upper manifold
QuixStand Operating Instructions 28-9646-70 AA
25
3
3.3
Installation
Assembly
•
Support Rod
•
Accessory kit
•
Full assortment of clamps, gaskets, tubing, and fittings
•
Operating instructions
•
EC declaration of conformity
Depending upon which model you ordered, your system may also include a pump and
various types of pressure gauges and valves. If your system includes a peristaltic pump,
you also receive peristaltic pump tubing and a pump user guide.
•
Check the equipment for any apparent damage before starting installation.
•
Document any damage carefully and contact your GE Healthcare representative
Table 3-1. Equipment included with various QuixStand systems.
QuixStand system model No.
Pump included
Pressure gauge
type
Drain valve type
QSM-02S
No
Nonsanitary
Nylon
QSM-02SP
Yes
Nonsanitary
Nylon
QSM-02SP/50
Yes, 230 Volt
Nonsanitary
Nylon
QSM-03S
No
Sanitary
316LSS
QSM-03SP
Yes
Sanitary
316LSS
QSM-03SP/50
Yes, 230 Volt
Sanitary
316LSS
KCQSM-03SP
Yes
Sanitary
316LSS
QSM-04SA
No
Sanitary
316LSS
QSM-04SAP
Yes
Sanitary
316LSS
QSM-04SAP/50
Yes 230 Volt
Sanitary
316LSS
3.3 Assembly
The main assembly steps include the following:
26
1
Install the support rod and lower manifold.
2
Install a reservoir.
3
Install the cartridge.
4
Connect the pressure gauges, valves, and tubing.
QuixStand Operating Instructions 28-9646-70 AA
Installation 3
Assembly 3.3
5
Connect electrical power.
The following sections describe each step. Consult Figure 1-1 on page 10.
Install a support rod and lower
manifold
Follow these steps to install the support rod and lower manifold:
1
Insert one of the support rods into the hole on the lower manifold. Line up the hole
in the support rod with the hole in the back of the manifold and secure the rod in
place with the oval head screw.
2
Slide the upper manifold assembly onto the support rod and tighten the knob to
hold it in place.
3
Place the support rod cap on the support rod.
a)
If your system includes an optional peristaltic pump with a flat top, place the
pump on the lab bench and place the QuixStand on the pump.
b)
If your system includes a pump cover, place the pump cover on the lab bench,
place the pump under the pump cover, and place the QuixStand on top of the
pump cover.
c)
If you are using your own peristaltic pump, position the QuixStand so that the
pump inlet and outlet tubing can be connected without twisting or kinking.
Install a reservoir
Follow these steps to install a reservoir on the lower manifold:
1
Gather the following parts:
a)
A 400 ml or 1,000-ml reservoir.
b)
Two, 1.5 inch silicone gaskets.
c)
Two, 1.5 inch sanitary clamps.
d)
Reservoir cap and two downcomers.
2
Place a gasket on the 1.5 inch sanitary flange on the lower manifold. Place the
reservoir on top of the gasket and clamp it in place using a sanitary clamp (Figure
3-1).
3
Push the two downcomers into the holes on the bottom of the reservoir cap.
QuixStand Operating Instructions 28-9646-70 AA
27
3
3.3
Installation
Assembly
4
Place a gasket on the top of the sanitary flange on the top of the reservoir. Place
the reservoir cap on top of the gasket and secure it in place with a sanitary clamp.
Figure 3-1. Rod and reservoir installed on base assembly (left) and cap installed on top of reservoir
(right).
Install a cartridge
Cartridges with housing size 3M, 3X2M, 4M, and 4X2M use sanitary connectors to
connect to the upper and lower manifolds.
Cartridges with housing size 4 use a hose barb/o-ring arrangement to connect to the
upper and lower manifolds (Figure 3-2). The following two sections describe how to
install these two types of cartridges.
Figure 3-2. Cartridge with sanitary connector (left) and cartridge with hose barb connector (right).
Installing cartridges with
sanitary connectors
Follow these steps to install a cartridge with sanitary connectors at its ends:
1
28
Gather these parts:
QuixStand Operating Instructions 28-9646-70 AA
Installation 3
Assembly 3.3
a)
Cartridge size with 3M, 3X2M, 4M, or 4X2M housing.
b)
Two, ½-inch silicone gaskets.
c)
Two, fractional sanitary clamps.
2
Raise upper manifold so that distance between upper and lower manifolds
exceeds cartridge length.
3
Place a gasket on the lower manifold.
4
Place the end of the cartridge on the gasket in the lower manifold and secure with
a sanitary clamp.
5
Place a gasket on upper end of cartridge.
6
Loosen the upper manifold and slide it down to mate with gasket and upper end of
cartridge.
7
Tighten upper manifold hand knob to secure it in place.
8
Secure the end of the cartridge to the upper manifold with a sanitary clamp.
Tip:
Two types of 1-inch (nominal) gaskets are included with QuixStand systems. The
gaskets have similar outside diameters but different inside diameters. When
installing the sanitary pressure gauges on models QSM- 03S and QSM-03SP, use
the gaskets having a larger nominal inside diameter. The larger inside diameter
prevents interference with the pressure gauge’s diaphragm.
Installing cartridges having
hose barb connectors
Follow these steps to install cartridges with 3/8 inch hose barb end fittings:
1
Gather these parts:
a)
Cartridge with size 4 housing.
b)
Two, ½ inch silicone gaskets.
c)
Two, fractional sanitary clamps.
d)
Two, cartridge-to-manifold adaptors.
e)
Two, long tube standoffs.
f)
Two, hose barb O-rings.
g)
Two, short tube standoffs.
2
Raise the upper manifold so that distance between upper and lower manifolds
exceeds the cartridge length.
3
Clamp the hose barb adaptors to the upper and lower manifolds using the silicone
gaskets and sanitary clamps.
QuixStand Operating Instructions 28-9646-70 AA
29
3
3.3
Installation
Assembly
4
Push a long tube standoff onto each cartridge hose barb (Figure 3-3).
5
Push an o-ring onto each cartridge hose barb and seat it against the standoff.
6
Push a short tube standoff onto each cartridge hose barb and seat it against the
o-ring.
7
Wet the o-rings with water, and with the standoffs and o-rings in place, use a
twisting motion and firmly slide the lower end of the cartridge into the lower
manifold adaptor.
8
Slide the upper manifold assembly down to mate with the upper end of the
cartridge and press firmly.
9
Tighten upper manifold hand knob to secure it in place.
2
1
4
2
3
4
5
Figure 3-3. O-ring with long and short standoffs (left) and installation of a cartridge into adaptor
(right).
30
1
Adapter
2
Short standoff
3
O-ring
4
Long standoff
5
Cartridge
QuixStand Operating Instructions 28-9646-70 AA
Installation 3
Assembly 3.3
Connect the pressure gauges,
valves, and tubing
After installing the cartridge, connect the pressure gauges, valves, and tubing following
these steps:
1
Gather the following parts:
a)
Two pressure gauges.
b)
Drain/sample valve.
c)
Tubing supplied with the system (size 17 or 18).
d)
Accessory kit.
e)
Supplied clamps, gaskets, tubing, and fittings.
2
Attach one pressure gauge to the lower manifold and one pressure gauge to the
upper manifold using the ¾-inch silicone gaskets and sanitary clamps (Figure 1-1).
For models QSM-04SA and QSM-04SAP, position and clamp adaptors (fractional
sanitary fitting to 1.5-inch sanitary fitting) between the manifolds and the pressure
gauges so that the pressure gauges are positioned upward.
3
Attach the drain valve to the drain port located on the left side of the lower
manifold using a silicone gasket and sanitary clamp.
4
Install a length of tubing between the upper manifold and one of the hose barbs on
the reservoir cap. (This is the retentate line.) Ensure you slide the tubing pinch clamp
on the hose before installation. Secure the tubing at both ends with tubing clamps
to prevent leaks.
5
Install the sanitary-connector-to-hose-barb adaptors on the pump inlet port and
pump outlet port on the lower manifold using silicone gaskets and sanitary clamps.
Install a length of tubing from the reservoir outlet port, through the peristaltic pump
head, and on to the feed port. (Read the Watson-Marlow pump user guide to learn
how to install the tubing in the pump head.) Secure the tubing on the hose barbs
using tubing clamps.
6
Visually check the feed tubing upstream and downstream from the peristaltic
pump and ensure it is not pinched or held incorrectly. If necessary, loosen the
tension on the tubing and reposition.
7
Install a length of the tubing to the drain/sample valve hose barb using a tubing
clamp.
8
Install lengths of tubing from the upper permeate port on the side of the cartridge
to an appropriate collection vessel.
QuixStand Operating Instructions 28-9646-70 AA
31
3
3.4
Installation
Connections
3.4 Connections
Connect electrical power
The optional peristaltic pump motor can operate at 100-120 VAC (50/60 Hz) or 220-240
VAC (50/60 Hz). Ensure the voltage selector switch on the back of the pump is set to the
proper voltage before plugging it in. Consult the Watson-Marlow pump user guide if you
need help in setting the pump motor to the proper voltage. Connect the pump’s power
cord to a fuse-protected, electrical outlet of the proper voltage.
Final checks
Before using the system, check the flexible tubing to ensure it sits properly in the pump
head. Finally, check the tightness of all tubing clamps and sanitary clamps.
3.5 Spare parts and accessories
For correct up to date information on spare parts and accessories, visit
www.gelifesciences.com/
32
QuixStand Operating Instructions 28-9646-70 AA
Operation 4
Operation overview 4.1
4 Operation
4.1 Operation overview
Instructions for using hollow fiber cartridges are provided in the user guide supplied
with the cartridges. The actual operation of this system will vary considerably
depending upon your process and product objectives. If you need assistance with
application information, contact your GE Healthcare representative.
NOTICE
Do not allow ultra filtration cartridges to dry out. Dropping cartridges
during handling or subjecting the cartridge to pressure surges during use
can damage the hollow fibers or housing.
Tip:
Plot the recirculation rate for a given cartridge type (housing size and lumen size)
as a function of the pump speed to assist in data interpretation. To do this, attach
a flexible tubing line from the retentate outlet of the cartridge to a graduated
cylinder. Using a stop watch, measure the flow rate through the cartridge at
several pump speeds. Target pump recirculation rates for Xampler™ cartridges
are provided in Table 4-3 on page 37.
QuixStand Operating Instructions 28-9646-70 AA
33
4
4.2
Operation
Starting the instrument
4.2 Starting the instrument
WARNING
Using tubing not designed for peristaltic pumps in the peristaltic pump
can cause the tubing to fail prematurely, exposing the operator to
potentially hazardous process or cleaning fluids. To prevent the
premature or unexpected failure of the tubing, use special, heavy-duty
tubing intended for use in peristaltic pumps.
WARNING
If you start the feed pump with the retentate valve closed, the feed
pressure can reach excessively high pressure causing leaks, bodily
harm, and damaging the cartridge or cassette. To prevent leaks or
damage to system components, ensure the retentate valve is open
before starting the feed pump.
NOTICE
Starting the optional peristaltic pump with the voltage selector set to the
incorrect voltage can result in pump motor damage. To prevent pump
motor damage, ensure the voltage selector switch is set to the proper
voltage (110V/60 Hz or 220V/50 Hz) before starting the pump.
Pump operation
Before operating the optional peristaltic pump, you should read the pump
manufacturer’s user guide supplied with your QuixStand system.
1
4
2
6
7
3
5
Figure 4-1. Pump control panel for optional Watson-Marlow peristaltic pump showing the Start
and Stop buttons, other control buttons, and the display.
34
QuixStand Operating Instructions 28-9646-70 AA
Operation 4
Starting the instrument 4.2
Table 4-1. Parts in Figure 4-1.
Part
Description
1
Display
2
Start key
3
Stop key
4
Up arrow
5
Down arrow
6
Mode
7
Pump direction button
Pump controls
Read the user guide supplied with the Watson-Marlow peristaltic pump before
operating the pump. The pump control panel includes six control buttons and a display
(Figure 4-1). The control buttons perform these basic functions:
•
The control panel display indicates the pump speed in revolutions per minute
(rpm).
•
When the pump is running, pressing the Up Arrow button increases the pump
speed. Pressing the Down Arrow button decreases the pump speed. You can
adjust the speed on the display while the pump is stopped or running. Typically
you should start the pump at a very slow speed and increase the speed slowly.
•
Pressing the Pump Direction button reverses the direction of rotation of the pump
head when the pump is stopped or running.
•
Pressing the Start button starts the pump.
•
Pressing the Stop button stops the pump.
•
When stopped, the display continues to show the current setting for operating
speed and direction. When started, the pump returns to the displayed speed and
direction.
NOTICE
Failure to open the pump head and relieve the pressure on the tubing in
a peristaltic pump when the pump is not in use can accelerate tubing
wear. To prevent premature wear, open the pump head and relieve the
pressure on the tubing when the pump is not being used
QuixStand Operating Instructions 28-9646-70 AA
35
4
4.3
Operation
Starting the system
4.3 Starting the system
Initial start-up
Follow these steps to start-up the QuixStand system:
1
Close sampling/drain valve, secure cartridge in upper and lower manifolds, and
ensure the pump tubing is correctly positioned and tensioned within the pump
head.
2
Confirm flexible tubing is connected from the retentate outlet on the upper
manifold to one of the tubing barbs on the reservoir cap. If the process solution
tends to foam, ensure there is a retentate downcomer pressed into the reservoir
cap for the retentate line.
3
Direct the flexible tubing from the upper permeate line to a collection flask. If you
choose not to use the lower permeate line, close off the lower permeate port by
adding a short piece of tubing secured with a tubing connector or cable tie wrap
and clamp the tubing closed with a pinch clamp.
4
Remove the sanitary clamp from the reservoir cap and slide the reservoir cap to
one side.
5
Add the feed solution to the reservoir.
6
Reposition the reservoir cap on the silicone gasket and clamp it in place using the
sanitary clamp.
7
Open the backpressure tubing valve (turning counterclockwise), several turns.
8
Before staring the pump it is important to check the rotation direction of the pump.
The direction should be from the bottom of the reservoir to the filter cartridge.
9
Start the pump on slow speed, and wait about 30 seconds for the pressure to build
up. The pressure gauges on the sanitary and autoclavable system versions are
mechanically dampened and respond slowly. Slowly increase the pump speed. The
inlet pressure will build up, while the outlet pressure gauge may still read zero.
10 Apply backpressure by slowly closing the backpressure tubing valve.
11 Watch the inlet pressure gauge. If the pressure rises too high, lower the pump
speed.
Xampler cartridge
specifications
The cartridge selection guide available from your GE Healthcare representative
describes the specifications and recommended operating parameters for GE
Healthcare cartridges. For convenience, the specifications and recommended
operating flow rates for Xampler cartridges are provided below (Table 4-2, Table 4-3)
36
QuixStand Operating Instructions 28-9646-70 AA
Operation 4
Starting the system 4.3
Table 4-2. .Xampler cartridge specifications
Housing
size
Lumen
diameter (mm)
Membrane
area
(ft )
Hold-up
volume
lumen
(ml)
Shell
(ml)
Permeate port
(inches)
2
2
(cm )
3M
3×2M
4,4M
4×2M
0.5
140
0.15
2
9
1/4
0.75
120
0.13
3
8
1/4
1
110
0.12
3
5
1/4
0.5
290
0.31
5
14
1/4
0.75
260
0.28
6
14
1/4
1
110
0.12
3
5
1/4
0.5
650
0.7
8
45
3/8
0.75
460
0.5
10
40
3/8
1
420
0.45
12
30
3/8
0.5
1400
1.5
20
75
3/8
1
850
0.9
30
50
3/8
1
Table 4-3. Xampler cartridge nominal feed flow rates
Housing size
Lumen diameter (mm)
Shear sensitive (ml/min.)
Low fouling
(ml/min.)
High fouling
(ml/min.)
3M, 3×2M
0.5
60
120
250
0.75
100
200
400
1
150
300
600
0,5
300
600
1200
0,75
400
800
1500
1
600
1200
2500
4,4M, 4×2M
1
-1
-1
-1
Shear rates for shear sensitive = 2000 sec ; low fouling = 4000 sec ; high fouling = 8000 sec
Flow rate/pressure values determined with water will vary from experimental solutions due to
changes in temperature, viscosity and degree of suspended solids.
QuixStand Operating Instructions 28-9646-70 AA
37
4
4.4
Operation
Constant volume diafiltration
4.4 Constant volume diafiltration
The QuixStand system is ideally suited to achieve constant volume diafiltration as the
pump will pull a vacuum on the feed reservoir and draw in diafiltrate solution at a fixed
rate. Follow these steps to complete diafiltration:
1
Attach tubing from your diafiltrate solution to the hose barb on the reservoir cap.
2
Turn on the pump to circulate the process solution through the cartridge and draw
diafiltrate solution into the feed reservoir.
3
If the diafiltration solution causes foaming in the feed reservoir, ensure the second
downcomer is attached to the bottom of the reservoir cap and extends down
below the fluid level in the reservoir.
4.5 Pressurized feed reservoir
The QuixStand system may be operated with a pressurized feed reservoir. This mode of
operation is particularly useful in reaching very high concentration factors (minimizing
feed volumes for example). It is important to note that pressurization of the feed
reservoir is potentially hazardous and is not recommended unless the following
precautions are taken:
1
Limit the pressure of the air or nitrogen source to 1.7 barg (25 psig). Install a
pressure regulator.
2
Set these to limit the incoming pressure to 0.7 to 1 barg (10 to 15 psig) in the feed
reservoir.
3
When all safety equipment is installed, attach the air or nitrogen line to the tubing
barb on the reservoir cap.
Tip:
38
Expert users often follow these steps to enable the running of the system to the
minimum working volume:
Note that this is intended for microfiltration applications and not intended for
protein concentration applications.
(1) use a downcomer on the retentate return line and attach 3/8-in flexible tubing
to reach the bottom of the reservoir base, minimizing air entrainment;
(2) proceed with standard operation using the extended downcomer to the
minimum feed level possible;
(3) leave the pump tubing attached to the base assembly, but disengage the
tubing from the pump head;
(4) fully close the backpressure tubing valve; and
(5) operate the cartridge in a flow-through mode by applying low pressure air or
nitrogen as described above.
QuixStand Operating Instructions 28-9646-70 AA
Operation 4
High flow pump tubing (size 18) 4.6
4.6 High flow pump tubing (size 18)
The system is supplied with size 17 and size 18 pump tubing. Use size 18 when high
circulation rates are required.
4.7 Autoclaving
Some QuixStand system models and components can be autoclaved (Table 4-4). It is
important to understand the limitations of the system and/or the pressure gauges
before autoclaving.
General guidelines for autoclaving include loosening all clamps before autoclaving and
ramping the temperature up and down slowly. Consult the GE Healthcare hollow fiber
cartridge user guide for complete autoclaving instructions.
Table 4-4. Autoclavability of QuixStand systems and components
Model number
Autoclavable systems
Autoclavable pressure
gauges
No
No
No
Yes
Yes
Yes
QSM-02S
QSM-02SP
QSM-03S
QSM-03SP
QSM-04SA
QSM-04SAP
Tip:
Some users check cross-flow systems for leaks by pressurizing the system with
compressed air or water under pressure and observing any loss in air pressure
over time. Users also check the integrity of hollow fiber cartridges before use by
performing either a pressure-hold or air diffusion test. This may also be tested in
the segments between the pump and backpressure valve using water
pressurised by the pump. Do not exceed 15 psig (1 barg) of air or water pressure.
GE Healthcare publishes an integrity testing guide that describes how to
complete these tests.
4.8 Procedures after operation
The system should be flushed after each use. When you flush the membrane cartridge
after use, you simultaneously flush and clean the QuixStand system, thus no further
flushing is needed.
QuixStand Operating Instructions 28-9646-70 AA
39
4
4.8
Operation
Procedures after operation
After use, you should wipe down the exterior components of the QuixStand system with
water to remove dirt and residual process/cleaning solutions. Wipe the system dry with
a clean, lint free cloth.
40
QuixStand Operating Instructions 28-9646-70 AA
Maintenance 5
Inspection 5.1
5 Maintenance
WARNING
To prevent bodily injury, disconnect the power cable, disconnect the
compressed air line, and depressurize the system before performing
inspections and disassembling the system.
5.1 Inspection
The following inspections shall be performed:
•
Inspect and replace pump tubing as needed.
•
Inspect flexible tubing connections for secure fit.
•
Inspect sanitary clamps for tightness.
5.2 User maintenance schedule
The system should be flushed before storage.
When you flush the membrane cartridge after use, you simultaneously flush and clean
the QuixStand system, thus no further flushing is needed.
If the last operation you perform with the system is to flush the cartridge with a storage
solution, you must then flush the system and holder with DI water or WFI to remove the
storage solution.
Position an old cartridge or a short piece of flexible tubing with sanitary adaptors in the
cartridge position to flush the system with water.
After use, you should wipe down the exterior components of the QuixStand system with
water to remove dirt and residual process/cleaning solutions. Wipe the system dry with
a clean, lint free cloth.
5.3 Storing the system
To store the system for more than a few days, clean it as described in the maintenance
section. Drain all rinse water from the system. Remove the cartridge from the
QuixStand. Blank off open lines or ports in the system and reservoir. Store the cartridge
in accordance with the instructions in the cartridge user guide (in general, well-sealed
with ultra filtration membranes kept wet) and stored in cool or ambient temperature.
QuixStand Operating Instructions 28-9646-70 AA
41
5
5.3
Maintenance
Storing the system
System disposal
When the service life of the unit is reached, flush the system to remove hazardous
process fluids. Dispose of the unit in accordance with the government and
environmental regulations that pertain to your location.
42
QuixStand Operating Instructions 28-9646-70 AA
Troubleshooting 6
6 Troubleshooting
Symptom
Possible cause
Suggested remedy
Leaks at the tubing nipple at
permeate connection of
cartridge.
Inside dimension of tubing is
too large.
Ensure tubing i.d. is sized
properly for the connection.
Split tubing at barb Check
tubing for wear or splitting.
Replace tubing if necessary.
Tubing clamp incorrectly
installed.
Ensure the clamp is installed
and tight.
Process solution leaks from
sanitary connections.
Clamp is loose.
Check and tighten clamp.
Gasket worn, damaged,
improperly mounted or wrong
size
Inspect gasket for wear,
damage and proper sizing.
Replace if necessary.
Feed pressure increases from
starting values slowly at first,
then suddenly very rapidly,
exceeding process limit.
Blockage or partial blockage of
flow path.
Flush through to clean
blockage. If necessary replace
tubing and connectors. Please
refer to order information for
tubing and connectors.
Feed inlet at cartridge is
blocked.
Switch feed and retentate lines
to reverse flow and flush with
deionized water (DI) or water
for injection (WFI).
Fouled cartridge.
For feed flow rate versus
pressure drop and cleaning of
cartridge refer to Operating
Handbook - Hollow fiber
cartridges for membrane
separations.
Retentate valve closed or
restricted.
Open valve.
Speed of pump (RPM) is too
high.
Reduce pump speed.
No or low retentate pressure.
QuixStand Operating Instructions 28-9646-70 AA
43
6
Troubleshooting
Symptom
Possible cause
Suggested remedy
Feed pressure is too low.
Pump head is not turning.
Check for error messages on
pump display, check voltage
setting is correct on power
supply and power is delivered
to the motor.
Pump is turning but flow is
inadequate.
Check occlusion setting on
pump (if applicable).
Tubing has become drawn
inside the pump head and has
flattened within pump head.
Open pump head and stretch
tubing while at the same time
closing the pump head. Check
to see that the settings are
correct on the tubing gates on
either side of the pump head
for the tubing installed. Refer to
Pump Manufacturer's
instructions for details if
applicable.
Air leaking from cartridge or
housing during cartridge
integrity testing (Cartridge
integrity testing employs air on
the retentate side of a fully
wetted cartridge).
44
Cartridge not wetted properly.
Cartridge not been wetted
properly. Please read the
chapter New cartridge rinsing
procedure in the Operating
Handbook - Hollow fiber
cartridges for membrane
separations. For pre-treatment
with alcohol please read the
document Hollow fiber
cartridge preparation
instructions.
Membrane is not integral.
Please contact your local GE
Healthcare technical support
representative.
QuixStand Operating Instructions 28-9646-70 AA
Troubleshooting 6
Symptom
Possible cause
Suggested remedy
Water leaking during system
integrity testing (System
integrity testing employs water
on the retentate side).
Faulty diaphragm valve.
Get valve serviced by GE
Healthcare service engineer or
replaced.
Gasket worn, damaged,
improperly mounted or wrong
size.
Inspect gasket for wear,
damage and proper sizing.
Replace if necessary.
Leakage from process
connectors.
Check and tighten connectors.
Air trapped in the pore
structure of the membrane.
Air-locked membrane, in which
case, water layers trap a
pocket of air between inner
and outer surfaces of the
membrane; effectively
reducing the available surface
area. This may be observed
following autoclaving. Remedy:
recirculate cold water (5-15C)
on the retentate side with the
permeate side closed, very
gradually increase retentate
backpressure until a stable 20
psig is reached and hold this
condition for 10 minutes or
until expected permeate flux is
reached Note that this does not
affect feed pressure or TMP;
due to the permeate side being
closed, but only raises the
absolute pressure on both
sides of the membrane;
causing the trapped air to
diffuse rapidly into the water.
Permeate flow is not adequate
(microfiltration membrane).
QuixStand Operating Instructions 28-9646-70 AA
45
6
Troubleshooting
Symptom
Possible cause
Suggested remedy
Permeate flow is not adequate
(ultrafiltration membrane).
Air trapped in the pore
structure of the membrane, or
glycerol humectant not
properly removed.
Cartridge not wetted properly.
Please read the chapter New
cartridge rinsing procedure in
the Operating Handbook Hollow fiber cartridges for
membrane separations. For
pre-treatment with alcohol
please read the document
Hollow fiber cartridge
preparation instructions.
Auxiliary equipment such as
manometers and pumps not
working properly.
Check the function of all
auxiliary equipment. Repair/
replace if necessary.
Bent tubing.
Check that the flow path is not
restricted.
Liquid viscosity too high.
Check the viscosity of the
liquid. Viscosity is a function of
temperature. (Lower
temperature gives higher
viscosity.) Let low-temperature
liquid reach operating
temperature before starting
the run.
Microbial growth in buffers. The
buffer normally becomes
opalescent due to microbial
growth.
Check buffers, especially those
with phosphate, for microbial
growth. Replace with fresh
buffer if necessary.
Dimension of tubing is
incorrect.
Ensure tubing i.d. is sized
properly for the connection.
Fouled cartridge.
Clean the cartridge according
to instructions.
Split tubing at barb Check
tubing for wear or splitting.
Replace tubing if necessary.
Tubing clamp incorrectly
installed.
Ensure the clamp is installed
and tight.
Permeate pressure is dropping.
46
QuixStand Operating Instructions 28-9646-70 AA
Troubleshooting 6
Symptom
Possible cause
Suggested remedy
Unexpected passage of target
protein to the permeate side.
Membrane pore size is too
large for target molecule.
Select the largest pore size that
retains the target molecule (in
general choose a membrane 35× smaller than the target
protein). For example, either
30,000 or 50,000 NMWC
ultrafiltration membranes are
recommended for mAb
concentration and diafiltration.
Unexpected retention of target
molecule.
Membrane pore size is too
small for target molecule.
Select the smallest pore size
that still allows efficient
passage of the target molecule
(in general 10 x larger opening
than the target protein) For
most mammalian cell culture
clarification 0.2 to 0.65 micron
pore sizes are used.
QuixStand Operating Instructions 28-9646-70 AA
47
6
48
Troubleshooting
QuixStand Operating Instructions 28-9646-70 AA
Reference information 7
Specifications 7.1
7 Reference information
7.1 Specifications
Size and dimensions
Part
Dimensions (W × L × H)
Short rod without pump
24 × 34 × 55 cm (9.5 × 13.5 × 22 in.)
Short rod with pump
24 × 34 × 70 cm (9.5 × 13.5 × 28 in.)
Long rod without pump
24 × 34 × 93 cm (9.5 × 13.5 × 37 in.)
Long rod with pump
24 × 34 × 108 cm (9.5 × 13.5 × 43 in.)
Weight, approximate
System
Weight
Without pump
12 kg / 25 lbs
With pump
18 kg / 40 lbs
Construction materials- Wetted
parts
Part
Dimensions (W × L × H)
QSM-02S
Polysulfone, BioPrene®, Nylon drain
valve, 316L SS gauges
QSM-03S
Polysulfone, BioPrene®, 316L SS drain
valve and gauges
QSM-04SA
Polysulfone, BioPrene®, 316L SS drain
valve and gauges
All systems supplied with silicone gaskets and O-rings.
QuixStand Operating Instructions 28-9646-70 AA
49
7
7.2
Reference information
Ordering information
Operating parameters
Parameter
Value
Recommended operating temperature
5 to 40°C (41 to 104°F)
Maximum temperature, short term
50°C (120°F)
Recommended maximum inlet
pressure
1.7 barg (25 psig)
Operating pH range
1 to 14
Reservoir volume
400 ml, 1,000 ml and optional 2,500 ml
Minimum working volume
30 to 50 ml
Parameter
Value
Input voltages and frequency
110-240 VAC, 50-60 Hz
Maximum temperature, operating
40°C (104°F)
Maximum temperature, short-term
(cleaning)
50°C (120°F)
Flow rate at 400 rpm
2 liters/min. with size 18 tubing
Pump
1.4 liters/min. with size 17 tubing
See the Watson-Marlow pump user guide for additional details.
7.2 Ordering information
Visit www.gelifesciences.com for ordering and spare parts information.
50
QuixStand Operating Instructions 28-9646-70 AA
For local office contact information, visit
www.gelifesciences.com/contact
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
www.gelifesciences.com/filtration
GE, imagination at work and GE monogram are trademarks of General
Electric Company.
QuixStand is a trademark of GE Healthcare companies.
All third party trademarks are the property of their respective owners.
© 2010 General Electric Company—All rights reserved.
First published Feb. 2010.
All goods and services are sold subject to the terms and conditions of sale
of the company within GE Healthcare which supplies them. A copy of these
terms and conditions is available on request. Contact your local GE
Healthcare representative for the most current information.
GE Healthcare UK Ltd
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare Japan Corporation
Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan
imagination at work
28-9646-70 AA 02/2010