Cat Nr/REF: KB-10107
Edition 19.05.06
English
For professional use only
Poseidon DLEU (13q14) & p53 (17p13) Probe
Introduction:
Deletion at 13q involving the band q14 occurs frequently in B-cell chronic lymphocytic
leukaemia (CLL) and Multiple Myeloma. A minimal critical region has been shown to lie
between the RB1 gene and the marker D13S25 containing DLEU1, DLEU2, and RFP2
genes.
Alterations of the p53 gene (TP53, 17p13) occur not only as somatic mutations in human
malignancies, but also as germline mutations in some cancer-prone families with LiFraumeni syndrome. Deletions of p53 are frequent in CLL and MM, usually associated
with unfavorable prognosis.
Intended use:
KB-10107
ON DLEU (13q14) / p53 (17p13)
The DLEU (13q14) specific DNA Probe is optimized to detect copy numbers of the
DLEU gene region at 13q14.
The p53 (17p13) specific DNA region is optimized to detect copy numbers of the p53
gene region at 17p13.
The probe is recommended to be used in combination with a Poseidon FISH Kit
providing necessary reagents to perform FISH (KB-60002, KB-60003 or KB-60001) for
optimal results.
Critical region 1 (red):
The DLEU (13q14) specific DNA probe is direct-labeled with PlatinumBright550.
Critical region 2 (green):
The p53 (17p13) specific DNA probe is direct-labeled with PlatinumBright495.
Reagent:
Poseidon probes are direct-labelled DNA probes provided in a concentrated format. For
FISH this probe needs to be diluted with the supplied hybridization buffer. For
hybridization use 2 parts of probe + 8 parts of Cell Hybridization Buffer (CHB).
Please refer to the Instructions for Use for the entire Poseidon FISH protocol.
Note: For tissue hybridization use THB (Tissue Hybridization Buffer) and
KREABoost – available on request.
Interpretation:
Published October 2006 poseidon@kreatech.com
Red probe
Probe map:
The DLEU (13q14) & p53 (17p13) probe is designed as a dual-colour assay to detect
deletions at 13q14 and 17p13. Deletions involving the DLEU gene region will show one
red signal and two green signals for the p53 region at 17p13 (1R2G). Deletions involving
the p53 gene region will show one green signal and two red signals for the DLEU region
at 13q14 (2R1G).
Deletions involving both critical regions at 13q14 and 17p13 will show one red and one
green signal only (1R1G). Two single colour red (R) and green (G) signals will identify
the normal chromosomes 17 (2R2G).
Normal Signal pattern
Expected signals
2R2G
Del(13q14)
1R2G
Del (17p13)
2R1G
Del (13q14)(17p13)
1R1G
Note: In CLL patients also Nullosomie for 13q14 may occur. Using this probe a signal
pattern of two green signals (2G) will be observed.
References::
Amiel A et al, 1997, Cancer Gener.Cytogenet, 97; 97-100
Drach J et al, 1998, Blood, 92; 802-809
Stilgenbauer S et al, 1998, Oncogene, 16; 1891 – 1897
Wolf S et al, 2001, Hum. Molec. Genet., 10; 1275-1285
Not to scale
Green probe