Lab 2 Assignment Due at the beginning of Lab 4

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Lab 2 Assignment Due at the beginning of Lab 4 worth 3%
____/20 points
Analysis of the antimicrobial results: do individually, not as a team.
Remember your name & last 4 digits of your student # on the cover page
(Reprinted from Lab 2 pages 2-6 & 2-7)
Only the stated maximum length will be read and marked so do not exceed these
limits.*
1. Table 1: your raw data (-2 if not included) and list the first names of the others in your
team.
2. (4 points) Graph data (collected in Table 1) comparing the zone of inhibition for the 4
antimicrobials:
Figure 1 antibiotics
Figure 2 disinfectants and antiseptics
Figure 3 spices
Figure 4 # colonies growing in the zone of inhibition
3. (16 points total) Discuss the meaning of the results (maximum 2 typed pages*) Use
the same subheadings in your assignment:
(1 point) State your original hypothesis and prediction of bacterial sensitivity to
antimicrobials in general.
(3 points) State which type of antimicrobial you predicted would be the MOST
effective and LEAST effective and briefly explain why.
(4 points) Discuss the meaning of any colonies growing in a zone of inhibition—
did your plates have any? How did the number of colonies vary for the different
antimicrobial substances? (If none of your plates had colonies growing in the
zone of inhibition, discuss what these colonies would mean.)
(8 points) Based on YOUR data which antibiotic, disinfectant, antiseptic, and food
spice are the MOST effective and which are the LEAST effective to kill E. coli?
Discuss the implications for your findings for each type of antimicrobial.
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