We’ve got you
covered.
The VeraCode® ADME Core Panel.
CYP2D6*8
CYP2C9*8
CYP2C9*5
Potential lifesaving drug?
Or a blind alley?
That’s what you need to find out
during pharmacogenetic studies.
Relying on the VeraCode ADME
Core Panel and the BeadXpress®
Reader will help you assess the
genetics behind drug absorption,
distribution, metabolism, and
excretion (ADME). Investigating
associations to drug response,
dosing, and safety.
Helping you introduce drugs
to market sooner. Bringing
personalized medicine closer
to reality.
Quickly. Efficiently. Precisely.
Quality counts.
Not only does the VeraCode ADME Core Panel provide more
than 95% of key ADME Core content, it also delivers the highest
quality data. More than 99.5% concordance. More than
99.5% reproducibility.
Plus, Illumina assay chemistry precisely targets critical ADME
content, addressing homologous regions. The information you get
will be more precise. The decisions you make more informed.
Precisely addressing homologous regions
100%
97%
100%
2C18 100% 2C19
2C19
2C18
100%
2C9 100% 2C8
2C9
2C8
100%
97%
SNP
SNP
Region-specific primer
Region-specific
primer
Extended
product
ExtendedAssay
product
oligos
Assay oligos
Genotyping Assay
Genotyping Assay
CYP2C9*2 (R144C)
CYP2C9*2 (R144C)
)
)
The CYP450 gene family includes
3
a tetrad of closely related genes:
3
CYP2C18, CYP2C19, CYP2C9 and
2
CYP2C8. Using an upfront targeting
Norm R
Norm R
2
approach, a specific gene of interest
1
can be pulled out from the genome,
1
G/G
0
0
23
0
23
0
0.20
7
7
0.20
0.40
0.40
0.60
0.60Norm Theta
0.80
Norm Theta
1
1
G/T
G/T
T/T
T/T
0.80
1
avoiding other homologous regions,
G/G
1
Selected
and can be analyzed precisely with
Selected
excellent discrimination.
More of what’s
important.
Make your metabolism studies more efficient. Get drugs to
market faster. Improve human health.
The VeraCode ADME Core Panel offers a better path to metabolism
studies. The most complete coverage of critical content. Highest
quality data. Simplest, fastest workflow. More core.
ADME Core Genes (34 genes)
ABCB1
CYP2C19
DPYD
SLC22A1
TPMT
ABCC2
CYP2C8
GSTM1
SLC22A2
UGT1A1
ABCG2
CYP2C9
GSTP1
SLC22A6
UGT2B15
CYP1A1
CYP2D6
GSTT1
SLCO1B1
UGT2B17
CYP1A2
CYP2E1
NAT1
SLCO1B3
UGT2B7
CYP2A6
CYP3A4
NAT2
SLCO2B1
VKORC1
CYP2B6
CYP3A5
SLC15A2
SULT1A1
The essential genes that populate the VeraCode ADME Core Panel
include several with well-known associations to model drugs. For
example, CYP2D6 has been associated with the metabolism of certain
antidepressants, antipsychotics, cancer drugs like Tamoxifen and many
others, CYP2C19 has been associated with the metabolism of the
anti-platelet Plavix, and CYP2C9 and VKORC1 are most well known
for their association to warfarin sensitivity.
For a detailed VeraCode ADME Core SNP list, please contact your
account manager or visit www.illumina.com/veracodeADME.
Speed matters.
One day. Why wait longer when you can analyze key ADME
content in under 8 hours? The simple workflow, with builtin sample tracking and internal controls, accelerates your
studies. The easy-to-use software interface manages a
run from setup through data analysis and translation.
It even provides useful reports.
Workflow
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go go go
Oli Oli Oli
Universal PCR
Single Plate Transfer
Targeting Reaction
Extension and
Ligation Reaction
Hybridization
Pre-PCR (2 hr 15 min)
Scan
Post-PCR (5 hr 15 min)
Simple workflow.
An initial extension with biotinylated primers specifically
targets regions of interest. Products are captured onto
paramagnetic particles for allele-specific primer extension
and ligation followed by universal PCR. A single plate
transfer hybridizes amplified material to VeraCode Beads for
scanning on the BeadXpress Reader. Thirty-four genes are
assayed within 8 hours with less than 2.5 hours of technical
hands-on time. Quickly. Efficiently. Precisely.
Let the coverage begin. Learn more at
www.illumina.com/veracodeADME
Automatic Analysis
and Translation
Learn more at www.illumina.com/veracodeADME
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FOR RESEARCH USE ONLY
© 2010 Illumina, Inc. All rights reserved.
Illumina, illuminaDx, Solexa, Making Sense Out of Life, Oligator, Sentrix, GoldenGate, GoldenGate Indexing, DASL, BeadArray, Array of Arrays, Infinium,
BeadXpress, VeraCode, IntelliHyb, iSelect, CSPro, GenomeStudio, Genetic Energy, HiSeq, and HiScan are registered trademarks or trademarks of Illumina, Inc.
All other brands and names contained herein are the property of their respective owners.
Pub No. 182-2010-001 Current as of 19 March 2010