Journal of chnolog ote y an al of Na urn n Jo edicine & N om Nanomedicine & Nanotechnology ISSN: 2157-7439 Montasser et al., J Nanomed Nanotechnol 2016, 7:3 http://dx.doi.org/10.4172/2157-7439.1000383 Open Access Research Article A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms Montasser MS2*, Younes AM1, Hegazi MM1, Dashti NH2, El-Sharkawey AE3 and Beall GW4,5 Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia, Egypt Department of Biological Sciences, Faculty of Science, University of Kuwait, Kuwait 3 Nanoscopy Science Center, Faculty of Science, University of Kuwait, Kuwait 4 Department of Chemistry and Biochemistry, Texas State University, San Marcos, Texas, USA 5 Physics Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia 1 2 Abstract This is the first report on a rapid green synthesis of gold nanoparticles (GNPs) using red algae (Laurencia papillosa). The green synthesis of eco-friendly nanoparticles is of a great interest in nanoscience for biomedical applications and specifically for clinical diagnostic applications. GNPs especially nanoprism represents a new advanced tool to study cell function and useful in optoelectronics, in developing a drug delivery system to control plant virus diseases and in nanomedicine. Conventional physical and chemical methods have been developed for the synthesis of metal nanoparticles, but these methods are expensive and require the use of toxic and aggressive chemicals. In this paper, it is demonstrated that a rapid, low coast and eco-friendly method for synthesis of gold nanospheres and its conversion into gold nanoprisms has been developed. The method involves using water solvent extract of L. papillosa as a reducing agent. Nanoscopy and computational analysis revealed that the nanoprism and other different morphologies were obtained just by varying the concentration molarity of tetrachloauric acid (HAuCl4), keeping the concentration of pure algal extract (PAE) constant. In the same time, we perform ImageJ analyses for the size distribution of TEM images. The best concentration of AuCl4 was 5 mM and best concentration of the red algae extract was at 0.05 g/ml. The functional groups responsible for conversion of nanosphere into nanoprism were NH and OH groups found in the contents of the red algae extract. The as-synthesized gold nanoprisms were characterized by several physicochemical techniques. The nanoprisms are single crystalline, whose basal plates surface are atomically flat "111" planes. We anticipate our results to be a starting point for more applications in medicine, plant virus diseases, and industrial technology. Keywords: Gold nanoparticles; Nanospheres; Nanoprisms; GNPs; Red algae; Laurencia papillosa, Nanomedicine; TEM; FESEM; AFM; XRD; FTIR Introduction Currently, there is a growing need to develop environmentally benign nanoparticles synthesis process without using toxic chemicals in the synthesis protocols. The secrets gleaned from nature have led to the development of biomimetic approaches to the growth of advanced nano- materials. Gold nanoprism represents a new advanced tool to study cell function and useful in optoelectronics [1-3], in developing a drug delivery system using gene gun technology to control plant virus diseases and in nanomedicine [4]. The green synthesis of ecofriendly nanoparticle is of great interest in Nanoscience for biomedical applications [5]. This synthesis would be simpler, more economical and free of adsorbed chemicals on the surface of as- synthesized GNPs. Recently, several authors have been engaged in green synthesis of GNPs with different sizes and shapes [6-9]. The phytochemicals from the red algae served as an easy reducing and stabilizing agents for GNPs synthesis [10]. There are several important factors that affect the synthesis of nanoparticles, including pH of the solution, temperature, concentration of the extracts, size and shapes of GNPs [11]. Trace quantities of gold (Aureat, Au) and silver (Ag) nanoprism have been observed as by-products of the methods that predominately produce spheres nanoparticles [12]. Gold nanoprism have been used to study cell function and catalysis [13]. No published data are available about using the red algae Laurencia papillosa and the phytochemicals derived from them as a strong reducing and stabilizing agent in conversion of gold nanospheres into gold nanoprisms. The main objective of this work was to study the feasibility of using Laurencia papillosa assisted synthesis in production of gold nanosphere and the conversion into gold nanoprisms. J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Materials and Methods Tetrachloauric acid (HAuCl4), (Sigma, Aldrich chemicals, USA) was used without further purification. Initially different concentrations (Table 1) of HAuCl4 solution (0.25, 0.5, 1, 2, 3, 4 and 5 mM) were prepared in sterile water [14]. Samples of Laurencia papillosa were collected and then washed with fresh water repeatedly. Samples were air dried in the shade for 5 days, then ground into small pieces. Five grams of the dried samples were boiled in 100 ml sterile water for 5 min [15]. The boiled extract solution was centrifuged at 5000 rpm for 5 min at 5◦C. The centrifuged pellet was discarded and the supernatant (PAE) was used for the synthesis of GNPs. The GNP were produced in a one-step reaction, where each reaction had a total final volume of 4 ml with different concentrations of HAuCl4 each as shown in (Table 1). The PAE volume and concentration were kept constant, at room temperature. Bioreduction and optical properties The bioreduction and optical properties of the freshly prepared *Corresponding author: Montasser MS, Department of Biological Sciences, Faculty of Science, University of Kuwait, Kuwait, Tel: 96524985657; E-mail: magdy.montasser@ku.edu.kw Received November 09, 2015; Accepted June 17, 2016; Published June 22, 2016 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/21577439.1000383 Copyright: © 2016 Montasser MS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 2 of 10 Exp No. AuCl4 Conc.* (mM) Reaction vol./ml AuCl4 (10 mM Stock soln.) vol./ml Water vol./ml Total vol./ml of AuCl4 Stock conc. 1 0.25 4 1.25 48.75 50 2 0.5 4 2.5 47.5 50 3 1 4 5 45 50 4 2 4 10 40 50 5 3 4 15 35 50 6 4 4 20 30 50 7 5 4 25 25 50 * Concentrations of AuCl4 and PAE at 0.05 g/ml each. Total volume of 4 ml of AuCl 4 and PAE mixture formed by adding 2 ml of PAE to 2 ml of AuCl4. Table 1: Reaction conditions of GNPs synthesis using 10 mM of AuCl 4 at different concentrations. GNPs were investigated by measuring the UV-Vis spectrum between 200-800 nm in a 10-mm-path-length quartz cuvette with a 1 nm resolution (Agilent Cary UV-Vis NIR Spectrometer) [16]. Images of different as-synthesized GNPs were analyzed with Image J freeware to measure the particle size, size distribution and surface area [22-24]. Transmission electron microscopy (TEM) Results and Discussion TEM samples were prepared via drop casting on the carbon-coated grid [17]. TEM measurements were carried out on a JEOL model 1200EX instrument operated at an accelerating voltage 120 KV. Bioreduction and optical properties Atomic force microscopy (AFM) GNPs were prepared by solution-casting onto highly oriented graphite substrate and analyzed by AFM [18] in the contact mode on a VEECO digital instruments multimode. Field emission scanning electron microscopy (FESEM) Morphology of the as-synthesized GNPs was studied [19] using JSM-6763LA instrument Crystalline structure of resulted GNPs X-Ray Powder Diffraction (XRD) measurements of films of the assynthesized GNPs solvent cast onto glass slides were done on a Bruker AXS D8 Advance X-Ray Powder Diffractometer operating from 20 to 80° two theta at a voltage of 40 KV to determine the crystalline structure of GNPs [20]. The color of solutions listed in Table 1 changed from yellow to pale violet and finally ruby red, indicating the formation of GNPs (Figure 1a). There is an abrupt change in color between 2 mM and 3 mM. UVVis spectra recorded as a function of HAuCl4 concentration at room temperature showed an increase of a Surface Plasmon Resonance (SPR) band from 526 to 586 nm, clearly indicating the formation of GNPs [25-28] with different peak intensity as shown in Figure 1b. The absorption value at 526-586 nm was due to longitudinal excitation of the SPR vibration as resulted from the light scattering and absorption which is determined by the size of the GNPs, as the Plasmon band of GNPs can range from 510 to 560 nm [29]. It can be seen that there is a steady shift in the UV-Vis absorbance peak at 525 nm for 0.25 mM to 545 nm for 2 mM. The peak then shifts abruptly to around 570 nm and becomes broader at concentrations above 3 mM. The UV-Vis spectral trends confirmed the color changes observed visually. Before Reaction Functional group in PAE responsible for GNPs synthesis In order to determine the possible functional groups of the phytochemical present in PAE that help in the reduction of HAuCl4 to GNPs and its stabilization. Fourier transform infrared spectroscopy (FTIR) (RX spectrophotometer, Perkin Elmer, Waltham, MA) analysis was carried out After the removal of the free biomolecules that were not adsorbed by the nanoparticles after repeated centrifugation and redispersion in water, the nanoparticles were subjected to FTIR analysis [21]. After Reaction Oxidation state of the as-synthesized GNPs X-ray photoelectron spectroscopy (XPS) measurements were obtained on a KRATOS-AXIS 165 instrument equipped with dual aluminum-magnesium anodes using MgK radiation, operating at 5 KV and 15 mA with pass energy of 80 eV and an increment of 0.1 eV. The samples were degassed for several hours in the XPS chamber to minimize air contamination on the surface [21]. Computational analysis GNPs Size, Shape, and Distribution Analysis Using Image J and Statistical Software. Image J is a public domain, image processing program developed at the National Institutes of Health NIH. TEM J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Figure 1a: Change of absorbance of as-synthesized gold nanoparticles obtained from the reduction of AuCl4 Figure 1a: Change of absorbance of as-synthesized gold nanoparticles using Laurencia papillosa (increasing gold concentration moving from left to right). obtained from the reduction of AuCl4 using Laurencia papillosa (increasing gold concentration moving from left to right). Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 3 of 10 Figure 1b: UV-Vis Spectra of the Synthesized GNPs at Different concentration of AuCl4. Transmission electron microscopy (TEM) Field emission scanning electron microscopy (FESEM) Different concentrations of AuCl4-PAE resulted in various GNPs sizes of 3.5 nm up to 53 nm and various shapes of spherical, hexagonal, octagonal, triangular and truncated nanoprisms (Figure 2). The results suggested that the biosynthesis of triangular and truncated nanoprisms were formed in four steps: initiation, induction, growth and termination as shown in Scheme 1. In the initiation step, the spherical nanoparticles were seeded in different sizes. In the induction step, the spherical nanoparticles were aggregated into small triangular nanoprisms mixed with other crystal shapes. As the concentration of AuCl4 increased, the triangular and truncated nanoprisms grows into super crystals of nanoprisms (Figure 2). These results correlated with the fact that weak reducing agents can generally form bigger nanoparticles (nanoprisms) with different shapes. We observed a flower-like structure filled in with triangular crystals, as well as triangular structures filled in with artistically branched tree or map-like images (Figure 3). Also, it was noticed that biequilateral triangular nanoprisms and truncated nanoprisms were resulted from the conversion of nanosphere using various concentrations of AuCl4 as shown in Scheme 1: The resulted GNPs showed two types of triangular structures one was flat and the other was of a hopper growth type as shown in Figure 4. The morphology of hopper crystals arises when crystal growth at the edges is more rapid than the center growth. The hopper structure is anticipated to be used as a starting point for more applications in industrial technology and in medicine, especially in drug delivery systems since it represents a higher surface area per mass. 1. The computational analysis of TEM images showed two types of nanoprisms, one flat plate and the other type was hopper growth triangular nanoprism (Figure 4). In general, nanoprisms shapes represented ~90% of the total number of particles observed. This considered a significantly higher number than what was reported earlier [27]. The resulted GNPs ranged from 3.5 nm to 12 nm for concentrations less than 2 mM. However, triangular nanoprism ranged from 12 to 53 nm that often showed truncated nanoprism rather than what was reported previously [27]. At concentrations greater than 3 mM a bimodal distribution of particles appeared where there were two classes of particles. There was one class in the 30 to 50 nm range but there were also very large crystals that were hundreds of nm in size. The micrographs of the 5 mM best illustrate these phenomena. This change in particle size distribution again correlates with color changes and the UV-Vis spectra. J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Atomic force microscopy (AFM) AFM analysis showed 3D, Heights, Amplitude, and mixed images of GNPs including gold nanoprisms. The resulted surface indicated by a dotted line through triangular axis was relatively smooth and flat for the purely triangular particles but in the hopper crystals, the scan shows a distinct valley between the edges as shown in Figure 5. Crystalline structure of resulted GNPs The crystal structures of the as-synthesized gold nanoprism obtained after the reduction of HAuCl4 using Laurencia papillosa extracts were identified using X-Ray Diffraction (XRD) spectroscopy analysis (Figure 6). The Bragg peaks equivalent to "111", "200", "220" and "422" demonstrate the formation of crystalline gold nanoprism [30]. The intensity of the "111" line would indicate that the face of the triangular particles is the (111) type which is consistent with the trigonal symmetry. All reflections are distinctly indexed to a facecentered cubic (fcc) phase of GNPs. All other diffraction lines arise from the extracted algae components, byproducts of the reaction or substrate. These results corroborate with earlier published literature [28]. The presence of these four intense peaks corresponding to the nanoparticles was in agreement with the Bragg's reflections of gold identified with the diffraction pattern [29]. Thus, the XRD pattern suggests that the GNPs were essentially crystalline in nature. Hence, the simulated solution exhibited tremendous performance on the synthesis of GNPs as that of Laurencia papillosa extract. Similar results of XRD for GNPs synthesized using brown algae S. marginatum were found previously [30]. Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 4 of 10 Figure 2: TEM images with different concentration of AuCl4. A)0.25 mM, B) 0.5 mM, C) 1.0 mM, D) 2.0 mM, E) 3.0 mM, F) 4.0 mM, G) 5.0 mM and H) Average size and standard deviation of GNPs sizes at different concentrations of AuCl4 and different Size of GNPs. Upper right corner showing Gaussian Distribution and Error analysis by using Origin Pro 2015. According to Thresholding of TEM micrographs of different concentration using Imagej Software Analysis. Functional group in PAE responsible for GNPs synthesis In order to determine the possible functional groups of phytochemical or proteins in PAE that help in the reduction of HAuCl4 to GNPs and its stabilization, the FTIR spectroscopy analysis was J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal carried out. The major stretching frequencies of Laurencia papillosa extract were observed at 3396.03, 2126.13, 1644.98 and 625.78 cm-1 (blue line in Figure 7) whereas the stretching frequencies of GNPs were observed at 3396.99, 1644.02 and 673.03 cm-1 (green line in Figure 7). Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 5 of 10 A B Figure 3: Crystal and Super crystal of nanogold observed in TEM by using Imagej analysis showing a flower-like structure filled in with triangular super crystals (A), and triangular structures filled in with branched tree-/map-like images, and truncated triangular prisms including intensive 3D surface plot (B). J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 6 of 10 Figure 4: FESEM images of images two types of nanoprisms structures: Flat triangular, arrow (A), and(A), Truncated hopper hopper structures, arrow (B), close Figure 4: FESEM of two types of nanoprisms structures: Flat triangular, arrow and Truncated structures, arrow (B),up of single flat triangular structure (C), single hopperstructure truncated(C), triangular nanoprism (D), Plot Profilenanoprism Surface ofnanoparticles using ImageJ for the triangular close up of single flat triangular single hopper truncated triangular (D), Plot Profile Surface ofnanoparticles using structures (E) and truncated using intensive 3D surface plot ofstructures imagej analysis for flat triangular nanoprisms and aanalysis hopper for nanoprism (H). ImageJhopper for the structures triangular (F), structures (E) and truncated hopper (F), using intensive 3D surface plot (G) of imagej flat triangular nanoprisms (G) and a hopper nanoprism (H). J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 7 of 10 0 100 300 200 Distance (nm) Figure 5: AFM Images nanoprism structures: Triangular 3D B) Amplitude nanoprism, C) Height view nanoprism; andview aggregated Figure 5: AFMforImages for hopper nanoprism hopperA)structures: A) nanoprism, Triangular 3D nanoprism, B) Amplitude nanoprism, C) Height flower-like shape: D)and AFMaggregated Plot Profile,flower-like E) 3D, F)AFM Amplitude, AFM Height H) PlotAmplitude, Profile Surface using Image. nanoprism; shape: D) AFM G) Plot Profile, E) and 3D, F)AFM G) AFM Height and H) Plot Profile Surface using Image. J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 8 of 10 Figure 6: X-ray diffraction pattern of as-synthesized GNPs. Figure Figure 7: 7: FTIR FTIR Spectra Spectra of of Laurencia Laurencia papillosa papillosa extract extract (blue (blue line) line) and and as-synthesized as-synthesized GNPs. GNPs. The major IR spectrum arises at 3396.03 cm-1 in the blue line, due to the O–H stretching modes which are significantly reduced and become sharper upon coordination with GNPs as shown in Figure 7 green line [30], suggesting the role of phenolic groups in the reduction of HAuCl4 to GNPs. On the other hand, the IR spectrum at 1644.98 cm-1 (blue line) was due to the presence of amide in proteins of Laurencia papillosa [26]. The IR spectrum at 2126 cm-1 (blue line) due to the presence of C=O carbonyl group disappeared as indicated in green line due to the involvement of that protein in the reduction of HAuCl4 during the synthesis of GNPs Oxidation state of the as-synthesized GNPs X-ray photoelectron spectroscopy (XPS) data of gold nanoprisms shown in (Figure 8) are indicating the binding energy (BE) of Au atoms. J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal The BE was observed at both ~84, and 88 eV resulted in the presence of Au 4f7/2 and Au 4f5/2 respectively. The Au 4f7/2 core attributed to Au (0) or metallic gold (Figure 8). The presence of Au0 on GNPs surface helps to stabilize the GNPs from aggregations [30]. Computational analysis TEM images with different concentration and different Size of GNPs, Figure 2 showing thresholding of TEM in different concentration by ImageJ Software Analysis (Binary Contrast Enhancement). This is commonly used when detecting edges, counting particles or measuring areas. A grayscale image is converted to binary (a.k.a. halftone or black and white) by defining a grayscale cutoff point. Grayscale values below the cutoff become black and those above become white. Gaussian Distribution and Error analysis Volume 7 • Issue 3 • 1000383 Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 Page 9 of 10 Green line Figure8:8:XPS XPSspectrum spectrumofofAu Auatom atompresent presentininas-synthesized as-synthesizedGNPs. GNPs. Figure Scheme 1: Induction of gold nanospheres into gold nanoprisms using different AuCl4 Concentrations by using Origin Pro 2015 shown at the upper right corner of each TEM micrograph. Acknowledgements We gratefully acknowledge funding support for this research work from the Research Administration, Projects No. SL04/15 and SL06/14 and SL 08/15, Kuwait University, Kuwait. We extend our appreciations to Mr. Ahmed Mesalam Mohamed for his assistance, the Research Sector Projects Unit (RSPU) and Nanoscopy Science Center (NSC), Faculty of Science, Kuwait University, Kuwait. End Note 3. Boyd RW (1992) Nonlinear Optics, Academic Press, San Diego. 4. Singaravelu G, Arockiyamari JS, Ganesh KV, Govindaraju K (2007) Novel Extracellular Biosynthesis of Monodisperse Gold Nanoparticles Using Marine Algae, Sargassum Wightii Greville. Colloid Surf B: Biointerf 57: 97-101. 5. Vijayaraghavan K, Mahadevan A, Sathishkumar M, Pavagadhi S, Balasubramanian R (2011). Biosorption and subsequent bioreduction of trivalentaurum by a brown marine alga Turbinaria conoides. Chem Eng 167: 223-227. 6. Baker S, Rakshith D, Kavitha KS, Santosh P, Kavitha HU, et al. (2013) Plants: Emerging as Nanofactories Towards Facile Route in Synthesis of Nanoparticles. BioImpacts 3: 111-117. This is the first report on green synthesis of Gold nanoparticles (GNPs) using red algae (Laurencia papillosa). The green synthesis of eco-friendly nanoparticles is of great interest in Nanoscience for biomedical applications and specifically for clinical diagnostics applications. GNPs especially nanoprisms represents a new advanced tool to study cell function and useful in optoelectronics, in developing a drug delivery system to control plant virus diseases and in nanomedicine. 7. Kuppusamy V, Mahadevan A, Sathishkumar M, Pavagadhi S, Balasubramanian R (2011) Biosorption and Subsequent Bioreduction of Trivalentaurum by a Brown Marine Alga Turbinaria Conoides. Chem Eng J 167: 223-227. References 9. Rajeshkumar, Malarkodi C, Gnanajobitha G, Vanaja M, Paulkumar K, et al. 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Small 4: 1425-1436. 30.Shankar SS, Rai A, Ahmad A, Sastry M ( 2005) Controlling the optical properties of Lemongrass extract synthesized gold nanotriangles and potential application in infrared-absorbing optical coatings Chem. Mater. 17: 566-572. OMICS International: Publication Benefits & Features Unique features: • • • Increased global visibility of articles through worldwide distribution and indexing Showcasing recent research output in a timely and updated manner Special issues on the current trends of scientific research Special features: Citation: Montasser MS, Younes AM, Hegazi MM, Dashti NH, El-Sharkawey AE, et al. (2016) A Novel Eco-friendly Method of Using Red Algae (Laurencia papillosa) to Synthesize Gold Nanoprisms. J Nanomed Nanotechnol 7: 383. doi:10.4172/2157-7439.1000383 J Nanomed Nanotechnol ISSN: 2157-7439 JNMNT, an open access journal • • • • • • • • 700+ Open Access Journals 50,000+ editorial team Rapid review process Quality and quick editorial, review and publication processing Indexing at major indexing services Sharing Option: Social Networking Enabled Authors, Reviewers and Editors rewarded with online Scientific Credits Better discount for your subsequent articles Submit your manuscript at: http://www.omicsonline.org/submission Volume 7 • Issue 3 • 1000383