MLAB 2360 Clinical I
Serology/Hematology Activity
Activity 10: Hemoglobin Electrophoresis and Differentials to Diagnose Sickle Cell Anemia
STUDY QUESTIONS
Name __________________________
Date _______________________
Points
/15
Activity Grade:
Procedure
Study Questions
Points
Maximum
27
15
42
Student Points
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MLAB 2360 Clinical I
Serology/Hematology Activity
Activity 10: Electrophoresis and Differentials to Diagnose Sickle Cell Anemia
LABORATORY WORKSHEET
Name __________________________Date _______________________ Points
/27
The Adida family has immigrated to the United States from Nigeria. The child visited the doctor and was found to be
extremely anemic. Additional tests have been requested including a CBC and hemoglobin electrophoresis.
Patient information:
1. Mother: Sarah Adida, ID # 145679, date of birth 11-21-1988
2. Father: Franklin Adida, ID # 267348, date of birth 7-19-1986
3. Child: Joseph Adida, ID # 346128, date of birth 9-07-2007
Instructions
You have been given the automated CBC results for all patients. You are to perform the following tasks:
1. Set up patient samples for hemoglobin electrophoresis. There will be 3 patient samples and 3 controls:
a. Sickle cell DNA control – homozygous (S/S)
b. Sickle cell DNA control – heterozygous (A/S)
c. Normal DNA control (A/A)
2. While the gels are being electrophoresed you will perform differentials and evaluation of red blood cell morphology
on the three patients.
a. Normal differential should take 15 minutes
b. Abnormal differential should take 30 minutes
3. Compare electrophoresis results of patients with the controls and interpret the findings. Record the results below.
4. In the box below draw a picture of the results obtained from the gel LABELING EACH LANE.
5. IMPORTANT: Report results in the table at the bottom as follows: (1 point each, 9 total)
a. Homozygous S report out as “SS,”
b. Heterozygous S report out as “AS”
c. Normal report out as “AA”
Recording Control and Patient Results – Record Bands Observed for Each Sample
Ctrl
S homo
DATE
Ctrl
S hetero
Ctrl
Normal
Name
ID #
PATIENT NAME
PATIENT ID #
RESULTS
Initials
Time
CONTROLS
Sickle cell hemoglobin control – homozygous
Sickle cell hemoglobin control - heterozygous
Normal hemoglobin control
Results Grade: Date, initials, time 1 point each (3 total). Name, number, results 1 point each (12 total).
Patient and control results recorded in correct format 0.5 point each (3 points). (18 Total)
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MLAB 2360 Clinical I
Serology/Hematology Activity
Activity 10: Hemoglobin Electrophoresis and Differentials to Diagnose Sickle Cell Anemia
STUDY QUESTIONS
Name __________________________
Date _______________________
Points
/15
1. Based on the information provided is Sickle Cell disease a dominant or recessive trait? (1 point)
2. State the genotypes of the potential offspring for a couple with the following genotypes (2 points):
Mother: A/A
Father: S/S
Children:
3. State the amino acid substitution which causes the formation of hemoglobin S. (1 point)
4. State the disease for which individuals that are heterozygous for Hemoglobin S are resistant to. (1 point)
5. State the single base mutation for hemoglobin S. (1 point)
6. Describe the nucleotide sequence of normal b globin gene and the point mutation which occurs in codon 6.
(2 points)
7. State the palindrome recognition site for the restriction enzyme Mst II. (1 point)
8. State the genotype of each patient (A/A, A/S or S/S) ( 3 points):
a. Mother
b. Father
c. Child
9. State the phenotype of each patient ( Normal, Carrier or Sickle Cell) (3 points):
a. Mother
b. Father
c. Child
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