INDUCTIVE RESPONSE SUBJECTED TO PLANT GROWTH
REGULATORS IN ANTHER CULTURE OF VITIS SP.
*Tiffany Kerr, Tania Krastanova and Violeta Colova(Tsolova)
Center for Viticulture and Small Fruits Research, College of Engineering Sciences,
Technology and Agriculture, Florida A&M University, Tallahassee, Fl
*Presenting Author
Somatic embrogenesis (SE) can be induced in vitro from various plants’ tissues,
both generative and vegetative origin. Anthers are the preferred generative
explant in grape SE initiation. Embryogenic cells cultures are widely used for
micropropagation on a large scale for in vitro selection and various functional
genomics and gene transformation technologies. The objective of this study is
the SE initiation in anther culture of the grape rootstock variety “3309 Couderc”
for purpose of gene transformation as a model system for gene expresion.
Single node dormant cuttings ware used to force inflorences out of season under
greenhouse conditions in order to isolate the anthers and to induce embrogentic
callus. Somatic embryos from anthers were obtained after exposure of the
explants to 2.4 D/BAP growth regulators. A process of dedifferentiation, cell
division and embryogenesis were observed. Embryogenic lines will be
maintained both as clusters of differentiated somatic embryos through secondary
embryogenesis on solid medium and as proliferative Polyemryogenic Masses
(PEMs) in liquid cell suspension culture. High frequency of conversion of PEMs
to regenerated grape plantlets is expected to be achieved under the effect of the
dehydration of the culture and growth regulator treatments.
Key words: grape, somatic embryogenesis, regeneration, Vitis