General Biology Lab (Cell 211) Spring 2010 Instructor: Cherif Boudaba, Ph.D., cherif@tulane.edu; Phone: 862 8725 Office: 4014 Percival Stern Hall; Office hours: Monday from 10:00 to 12:00 or by appointment. Lab classes are held in room 3011, Percival Stern Hall. Class schedule: Depending on the section you enrolled in, your lab is Monday at 4:30 (cell 211-1), Wednesday at 4:30 (Cell 211-2) or Thursday at 3:30 (Cell 211-3). Prerequisite: CELL 101 and CHEM 108 Course description: This one-semester class is designed for majors in cell and molecular biology. This lab class focuses on general biology at macroscopic, microscopic and molecular levels. Textbook: Customized Biology Lab Manual, Sylvia S. Mader, tenth edition. Course learning outcomes: Upon completion of this lab class, successful students will be able to: - Use metric system and different lab techniques such as microscopy, DNA extraction, DNA fingerprinting and gene transfer. - Determine chemical composition of cells and understand their structure and function. - Understand the physical and chemical properties of an enzyme and relate it to digestion in mammals. - Understand mammal organization at tissue level and relate that to the function. - Describe the anatomy of nervous system and its physiology. - Explain DNA structure, describe the procedure of its isolation and its use in forensic. - Understand the relation between genes and traits (proteins). - Compare proteins encoded by a gene in its normal and mutated form. - Understand the transmission of genes from parents to offspring. - Understand the process of transferring a gene from an organism to another. Exams: The grades for CELL 211 lab classes are determined as follows: - Eight quizzes at 10 points each - Two midterms at 50 points each. Each midterm test includes the previously covered lab classes. Missed class: No makeup is given for any missed lab class or exam, except for sickness or death of an immediate family member. If one of two cases happens to a student, he or she should notify the TA and me as soon as possible. A makeup is given only for the quiz or the exam. If the missed lab class is still running that week, student may make it up with another group. Schedule of Labs Month Week of Topic January 11 Introduction to class, lab techniques, metric system 18 Martin Luther King Holiday No class 25 Chemical composition of cells Chapter 3 1 Cell structure and function Chapter 4 8 Enzymes Chemical aspects of digestion Chapter 5 Chapter 28 15 Mardi Gras No class 22 Midterm I 1 Human genetics Chapter 10 8 Animal organization Chapter 25 And chapter11 (page 143-146) 15 Nervous system and senses Chapter 30 22 DNA biology and technology Chapter 11 and handout 29 Spring break 5 Spring break 12 Transformation 19 Midterm II February March April Chapter in manual First pages of the manual Chapter 2 Handout