Phat L. Tran, Ph.D. Instructor Office Address: Texas Tech University Health Sciences Center Department of Ophthalmology and Visual Sciences School of Medicine 3601 4th Street STOP 7217 Lubbock, TX 79430-7217 Work: 806.743.2480 Email: phat.tran@ttuhsc.edu Education August 2002 to May 2008 Ph.D. Medical Microbiology and Immunology Texas Tech University Health Sciences Center, Lubbock, TX August 1996 to August 1999 B.A. Biochemistry; Biology Minor. Honors College with Highest Honors Texas Tech University, Lubbock, TX August 1996 to May 1999 B.S. Cell and Molecular Biology, Chemistry Minor Texas Tech University, Lubbock, TX June 1994 to August 1996 Non-degree Amarillo College, Amarillo, TX Academic Appointments June 2012 to Present Instructor Ophthalmology & Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX Positions and Employment June 2008 to May 2012 Post Doctoral Research Associate Texas Tech University Health Sciences Center, Lubbock, TX September 2002 to May 2008 Graduate Student Texas Tech University Health Sciences Center, Lubbock, TX September 1999 to August 2002 Medical Research Technician Texas Tech University Health Sciences Center, Lubbock, TX June 1997 to August 1999 Fellow The Howards Hughes Medical Institute (HHMI), Texas Tech University, Lubbock, TX Other Experience and Professional Memberships 1 2012 to Present Wound Healing Society 2011 to Present The Association for Research in Vision and Ophthalmology 2009 to 2010 American Society for Biomaterials 2002 to 2008 American Society of Microbiology 1997 to 2002 American Society of Cell Biology Awards and Honors Texas Tech University, Lubbock, TX 1999 B.A. Awarded with Highest Honors 1996 to 1999 Texas Tech University Honors Texas Tech University, Lubbock, TX Program 1999 The Honors Dean Award Texas Tech University, Lubbock, TX 1999 The Golden Key Honors Society Award Texas Tech University, Lubbock, TX 1998 to 1999 The Chancellor Endowed/TTU Honors Scholarship Texas Tech University, Lubbock, TX 1997 to 1999 The Howard Hughes Medical Institute Fellow Texas Tech University, Lubbock, TX 1997 to 1999 The H. Y. Price, Jr. Memorial Scholarship Texas Tech University, Lubbock, TX 1996 to 1999 Phi Theta Kappa International Honors Society Texas Tech University, Lubbock, TX 1996 to 1999 The National Dean’s List Awards Texas Tech University, Lubbock, TX 1996 to 1998 The Phi Theta Kappa Scholarship Texas Tech University, Lubbock, TX 1996 to 1997 The Texas Tech University Scholarship Texas Tech University, Lubbock, TX 1996 The CRC Press Chemistry Achievement Award Amarillo College, Amarillo, TX 2 1996 The Outstanding Major for Pre-med Award Amarillo College, Amarillo, TX Funding Grants The South Plains Foundation Grant Organo-selenium containing dental sealant inhibits biofilm formation by oral bacteria Period: 11/10/2015 to 08/31/2016 Amount: $10,500 Role: Principle Investigator SBIR Phase II Selenium Biopolymer Spacers to Prevent Biofouling of Reverse Osmosis Modules Period: 3/15/2013 to 2/28/2015 Amount: $499,999 (Sub-award $43,002) Role: Co-Principle Investigator NASA NNX14CJ10P, 14-1 H3.04-9932 Organoselenium Surface Modification of Stainless Steel Surfaces to Prevent Biofouling in Treatment of Space Wastestreams Period: 09/01/2014 to 12/19/2014 Amount: $45,000 Role: Principle Investigator Correctional Healthcare Research Fund Organo-selenium containing polyurethane foam dressing inhibits Staphylococcus aureus growth on wounds Period: 07/01/2013 to 09/30/2014 Amount: $10,000 Role: Principle Investigator South Plains Foundation Grant Organo-selenium containing polyester bandage inhibits Staphylococcus aureus growth on wounds Period: 09/01/2013 to 08/31/2014 Amount: $13,500 Role: Principle Investigator Bibliography and Publications Publications 1. Tran, Phat L.; Huynh, Eric.; Pham, Patrick.; Jarvis, Courtney.; Mosley, Thomas.; Hamood, Abdul N.; Hanes, Rob.; Reid, Ted W. 2016. An Organo-Selenium Polymer Inhibits Biofilm Formation in a Contact Lens Case. Manuscript accepted. Eye & Contact Lens Journal. 2016 Mar 11. [Epub ahead of print]. 2. Phat L. Tran, PhD; Eric Huynh, BS; Abdul N. Hamood, PhD; Anselm de Souza, PhD; Gregory Schultz, PhD; Bernd Liesenfeld, PhD; Dilip Mehta, PhD; Ted W. Reid, PhD. 2015. The ability of quaternary ammonium groups attached to a urethane bandage, to inhibit bacterial attachment and biofilm formation in a mouse wound. International Wound Journal. International Wound Journal. Article first published online: 28 DEC 2015. doi: 10.1111/iwj.12554. 3. Debdeep Banerjee, AB; Phat L. Tran, PhD; Jane A. Colmer-Hamood, PhD; James C. Wang, PhD; Matthew Myntti, PhD; Joehassin Cordero, MD; Abdul N. Hamood, PhD. 2015. Effects of a Novel 3 Antimicrobial Agent on the Viability of Bacterial Biofilms on Tympanostomy Tubes. The International Journal of Pediatric Otorhinolaryngology. 79(11): 1909-1914. 4. Phat L. Tran, Abdul N. Hamood, Anselm de Souza, Gregory Schultz, Bernd Liesenfeld, Dilip Mehta and Ted W. Reid. 2015. A study on the ability of quaternary ammonium groups attached to a polyurethane foam wound dressing, to inhibit bacterial attachment and biofilm formation. Wound Repair and Regeneration. 23: 74–81. 5. Elmasri, Wael; Yang, Tianjiao; Tran, Phat; Hegazy, Mohamed-Elamir; Hamood, Abdul; Mechref, Yehia; Pare, Paul. Teucrium polium phenylethanol and iridoid glucoside identification and inhibition of biofilm forming Staphylococcus aureus. 2014. Journal of Natural Products. 78(1): 2–9. 6. Phat Tran, Saurabh Patel, Abdul Hamood, Tyler Enos, Thomas Mosley, Courtney Jarvis, Akash Desai, Pamela Lin, Ted Reid. 2014. A Novel Organo-Selenium Bandage That Inhibits Biofilm Development in a Wound by Gram-positive and Gram-negative Wound Pathogens. Antibiotics, 3(3): 435-449. 7. Miller, K. G., P. L. Tran, C. L. Haley, C. Kruzek, J. A. ColmerHamood, M. Myntti, and A. N. Hamood. 2014. "Next Science Wound Gel Technology, a Novel Agent That Inhibits Biofilm Development by Gram-Positive and Gram-Negative Wound Pathogens."Antimicrob Agents Chemother 58(6): 3060-72. 8. Phat L Tran, Abdul N Hamood, Ted W Reid. 2014. Antibiofilm Agents. From Diagnosis to Treatment and Prevention Series: Springer Series on Biofilms, Vol. 8. New York, NY. 9. Jiangping Bai, Ramasubramanian Bhagavathi, Phat Tran, Kendall Muzzarelli, Di Wang, Joe. A. Fralick. 2013. Evidence that the C-terminal region is involved in the stability and functionality of OprM in E. coli. Microbiological Research. 169(5-6): 425-31. 10. Tony Vercellino*, Phat Tran, Ted Reid, Abdul Hamood, Audra Morse. 2013. Evaluation of polymerized organo-selenium feed spacers to inhibit S. aureus and E. coli biofilm development in reverse osmosis systems. Membranes and Water Treatment. Desalination. 331:1-5. 11. James C. Wang, Phat Tran, Rob Hanes, Joehassin Cordero, John Marchbanks, Ted Reid, Abdul Hamood. 2013. Organoselenium-Coated Tympanostomy Tubes Inhibit Otopathogenic Biofilms. JAMA Otolaryngol Head Neck Surg. 139(10):1009-1016. 12. Vercellino T, Morse A, Tran P, Song L, Hamood A, Reid T, et al. 2013. Attachment of organoselenium to polyamide composite reverse osmosis membranes to inhibit biofilm formation of S. aureus and E. coli. Desalination. 309(0):291-295. Listed as Key Research Article in the study of Aquatic Sciences on Environment Progress. (http://environmentprogress.com/). June, 2013. http://environmentprogress.com/key-research-articles/attachment-of-organo-selenium-to-polyamidecomposite-reverse-osmosis-membranes-to-inhibit-biofilm-formation-of-s-aureus-and-e-coli/. 13. Vercellino T, Morse A, Tran P, Song L, Hamood A, Reid T, et al. 2013. The use of covalently attached organo-selenium to inhibit S. aureus and E. coli bio!lms on RO membranes and feed spacers. Desalination. 317: 142-151. 14. Tran P, Hamood A, Mosley T, Gray T, Jarvis C, Webster D, Amaechi B, Enos T, Reid T. 2013. Organo-Selenium Containing Dental Sealant Inhibits Bacterial Biofilm. Journal of Dental Research. 92(5): 461-466. 15. Vercellino T, Morse A, Tran P, Song L, Hamood A, Reid T, et al. 2013. Attachment of organoselenium to polyamide composite reverse osmosis membranes to inhibit biofilm formation of S. aureus and E. coli. Desalination. 309(0): 291-295. 16. Tran PL, Lowry N, Campbell T, Reid TW, Webster DR, Tobin E, Aslani A, Mosley T, Dertien J, Hamood JAN, Hamood AN. 2012. An organoselenium compound inhibits Staphylococcus aureus biofilms on hemodialysis catheters in vivo. Antimicrob. Agents Chemother. 56(2): 972-8. Listed as a featured article on Global Medical Discovery [ISSN 1929-8536] (http://globalmedicaldiscovery.com). October 15, 2012. 17. Tran PL, Lowry N, Campbell T, Reid TW, Webster DR, Tobin E, et al. 2012. An organoselenium compound inhibits Staphylococcus aureus biofilms on hemodialysis catheters in vivo. Antimicrob Agents Chemother 56(2): 972-8. 4 18. Nidadavolu P, Amor W, Tran P, Dertien J, Hamood JCA, Hamood AN. 2012. Garlic cream inhibits biofilm formation by bacterial pathogens from burn wounds. Journal of Medical Microbiology. 61(5): 662-671. 19. Low D, Hamood A, Reid T, Mosley T, Tran P, Song L, et al. 2011. Attachment of selenium to a reverse osmosis membrane to inhibit biofilm formation of S. aureus. Journal of Membrane Science 378(1-2): 171-178. 20. Reid T, Tran P, Mosley T, Tran K, Jarvis C, Patel S, Shashtri M, Hanes R, Hamood A. 2010. Biopolymers Containing Selenium: Their Synthesis And Use. International Journal of Medical Devices. 5(1): 23-36. 21. Tran PL, Hammond AA, Mosley T, Cortez J, Gray T, Colmer-Hamood JA, et al. 2009. Organoselenium coating on cellulose inhibits the formation of biofilms by Pseudomonas aeruginosa and Staphylococcus aureus. Appl Environ Microbiol 75(11): 3586-92. 22. Reid TW, Tran P, Spallholz J, Fralick J. 2003. Counter Terrorism Measures to Combat Yersinia Pestis with Selenium Pharmaceuticals. Proceedings of the 2002 Joint Service Scientific Conference on Chemical and Biological Defense Research. 1-7. Submitted 1. Paula Monaco, Phat Tran, Ted Reid, James Surles, Audra Morse. Performance of an organo-selenium coating in a long-term biofouling test using stainless steel pipes. Water Research Journal. 2015. 2. Paula Monaco, Phat Tran, Ted Reid, David Klein, Tony Vercellino, Audra Morse. Performance of covalently attached organo-selenium to RO feed spacers during fouling testing using real wastewater sources. Water Research Journal. 2015. 3. Phat L. Tran, PhD; Eric Huynh, BS; Abdul N. Hamood, PhD; Anselm de Souza, PhD; Dilip Mehta, PhD; Keith W. Moeller, ND; Cameron D. Moeller, ND;Ted W. Reid, PhD. The Ability of a Silver Colloidal Gel Dressing to Kill Bacteria in vitro and in vivo. Manuscript submitted. Journal of Medial Microbiology, 2015. 4. Daniel J Good, Michael Jansen, Jon R Kaufman, Sohrab Sidhu, Phat Tran, Thomas Mosley, Ted W Reid. Minimizing Risk and Improving Outcomes Using Selenium as a Trabeculectomy Adjunct. Journal of Glaucoma. 2015. 5. Amaechi BT, Tran PL, Hamood AN, Mosley T, Gray T, Jarvis C, Webster D, Reid TW. OrganoSelenium Containing Pit and Fissure Sealant Inhibit Bacterial Attachment to Teeth. Journal of American Dental Association. 2015. In Preparation 1. Huy Dong, Phat Tran, Keaton Luth, Pamela Lin, Akash Desai, Eric Huynh, David McCartney, Abdul Hamood, and Ted Reid. Endophthalmitis: Effective and cost saving antibiotic therapy. 2. Tran, Phat L.; Pham, Patrick.; Huynh, Eric.; Jarvis, Courtney.; Mosley, Thomas.; Hamood, Abdul N1.; Hanes, Rob.; Blake Lackey.; Reid, Ted W. Selenium Contact Lens Hydrogel Polymer: Inhibition of Both Gram-Negative and Gram-Positive Bacterial Biofilm Formation. Manuscript submitted. Eye & Contact Lens Journal. 2015. 3. Phat L. Tran, Abdul N. Hamood, Rob Hanes, Courtney Jarvis, Thomas Mosley, Ted W. Reid. An organo-selenium polyurethane dental water line which inhibits bacterial attachment. 2013. Manuscript submitted. 4. Phat Tran, Akash Desai, Keaton Luth, Eric Huynh, Patrick Pham, Huy Dong , Kelly Mitchell, Abdul Hamood, and Ted Reid. Betadine solution in not effective in inhibiting the growth of different Gram Negative and Gram Positive Pathogens in vitro. 5. Phat Tran, Tyler Enos, Abdul Hamood, Courtney Jarvis, Thomas Mosley, and Ted Reid. Selenium Coated Polyester Bandage inhibits the growth of Staphylococcus aureus. 5 6. Phat Tran, Tyler Enos, Abdul Hamood, Courtney Jarvis, Thomas Mosley, and Ted Reid. A study on the ability of an organo selenium compound, attached to a polyurethane foam wound dressing, to inhibit bacterial attachment and biofilm formation. 7. Phat L. Tran & Ted W. Reid. Synergistic Effect of Substance P with Insulin and Insulin-Like Growth Factor-I on Epithelial Migration of the Transformed Human Corneal Epithelial Cells (SV-40). 8. K. Mitchell, A Arnett, J.-C. Auten, P. L. Tran, T. Mosley, K. Tran, C. Jarvis, J. Thomas, R. Hanes, D. Webster, Leo Dominguez, A. Hamood, T. W. Reid. The use of a covalent selenium coating to inhibit biofilm formation on a silicone scleral buckle. 9. Tran, P. L., Zhao, X., Li, X., Reid, T, W., and Fralick, J. A. New selenium antimicrobials against Escherichia coli pYPR1 expressing the F1 antigen of Yersinia pestis. 10. Cassie Kruczek, Kokila Kakarala, Phat L. Tran, Tyler Enos, Ted W. Reid, Janet Dertien, Jane-Colmer A. Hamood, and Abdul N. Hamood. An organo-selenium cream inhibits biofilm formation by burn wound pathogens. Abstracts 1. Phat Tran, Abdul Hamood, Robert Hanes, Blake Lackey, and Ted Reid. Selenium Contact Lens Hydrogel Polymer: Inhibition of both Gram-Negative and Gram-Positive Bacterial Biofilm Formation. The Association for Research in Vision and Ophthalmology. May 3–7, 2015. Denver, CO. 2. Phat Tran, Ph.D., Tyler Enos, MD., Abdul Hamood, Ph.D., Courtney Jarvis, Ph.D., and Ted Reid, Ph.D. Selenium-coated polyester bandages inhibit the formation of bacterial biofilms in a wound. Symposium on Advanced Wound Care. April 29-May 03, 2015. San Antonio, TX. 3. Akash Desai, Phat Tran, Abdul Hamood, Kelly Mitchell, and Ted Reid. 2.5, 5, and 10% Betadine solution in not effective in inhibiting the growth of different Gram Negative and Gram Positive Pathogens in vitro. The Association for Research in Vision and Ophthalmology. May 3–7, 2015. Denver, CO. 4. James C. Wang, Debdeep Banerjee, Phat L. Tran, Joehassin Cordero, Jane. A. Colmer-Hamood, Abdul N. Hamood. During Middle Ear Infections, the Planktonic Growth of Pseudomonas aeruginosa provides the Anaerobic Conditions Required for the Development of Fusobacterium nucleatum Biofilm. American Society of Pediatric Otolaryngology. April 22-26, 2015. Boston, MA. 5. Debdeep Banerjee, Phat L. Tran, James C. Wang, Matthew Myntti, Joehassin Cordero, Abdul N. Hamood. Effects of a Novel Antimicrobial Agent on the Viability and Sustainability of Bacterial Biofilms on Tympanostomy Tubes. The Triological Society. Combined Sections Meeting. Jan 22, 2015. San Diego, California. 6. Debdeep Banerjee, Phat L. Tran, James C. Wang, Matthew Myntti, Joehassin Cordero, Abdul N. Hamood. Effects of a Novel Antimicrobial Agent, the Next Science, on the Viability and Sustainability of Bacterial Biofilms on Tympanostomy Tubes. The Triological Society. Combined Sections Meeting. January 22-24, 2015. Coronado Island, California. 7. Phat L. Tran, PhD; Abdul N. Hamood, PhD; Anselm de Souza, PhD; Gregory Schultz, PhD; Bernd Liesenfeld, PhD; Dilip Mehta, PhD; Daniel Webster, Ph.D ; Ted W. Reid, PhD. The ability of quaternary ammonium groups attached to a urethane bandage, to inhibit bacterial attachment and biofilm formation in a mouse wound. Symposium on Advanced Wound Care. October 16-18, 2014. Las Vegas, NV. 8. Eric Huynh, Patrick Pham, Phat Tran , Abdul Hamood, Kelly Mitchell, and Ted Reid. 5% Betadine solution in not effective in inhibiting the growth of different Gram Negative and Gram Positive Pathogens in vitro. The Association for Research in Vision and Ophthalmology. May 4–8, 2014. Orlando, FL. 9. Patrick Pham, Eric Huynh, Phat Tran, Abdul Hamood, Rob Hanes, and Ted Reid. Selenium Contact Lens Hydrogel Polymer: Inhibition of both Gram Negative and Gram Positive Bacterial Biofilm 6 Formation - A Study of Different Organo-Selenium Molecules. The Association for Research in Vision and Ophthalmology. May 4–8, 2014. Orlando, FL. 10. Phat Tran, Abdul Hamood, Thomas Mosley, Courtney Jarvis, Josh Thomas, Blake Lackey, and Ted Reid. Selenium Hydrogel Lenses: Effects Upon Bacterial Biofilm Attachment. The Association for Research in Vision and Ophthalmology. May 5–9, 2013. Seattle, WA. 11. Mitchell, K., Tran, P., Arnett, A.I., Mosley, T., Hanes, R., Jarvis, C., Hamood, A., Dominguez, L., and Reid, T.W. Organo-selenium coatings inhibit multiple species of biofilm formation on ophthalmic device material. Association for Research in Vision and Ophthalmology Meeting, May 6 – 10, 2012. Fort Lauderdale, FL. 12. Reid, T.W., Tran, P., Mosley, T., Jarvis, C., Thomas, J., Tran, K., Hanes, R., Hamood, A. Antimicrobial properties of selenium covalently incorporated into the polymer of contact lens case material. Association for Research in Vision and Ophthalmology Meeting, May 6–10, 2012. Fort Lauderdale, FL. 13. Phat Tran, Abdul Hamood, Thomas Mosley, Courtney Jarvis, Josh Thomas, Blake Lackey, and Ted Reid. Selenium Coated Contact Lenses: Effects Upon Bacterial Biofilm Attachment. The Association for Research in Vision and Ophthalmology. May 6–10, 2012. Fort Lauderdale, FL, 14. Tran, P., Mosley, T., Cortez, J., Shashtri, M., Spallholz, J., Hamood. and Reid, T.W. Bandages Coated with Organo-Selenium Inhibit Bacterial Attachment. Society for Biomaterials Meeting, February 21, 2010. College Station, TX. 15. Reid, T.W., Tran, P., Mosley, T., Cortez, J., Shashtri, M., Spallholz, J. and Hamood, Bandages Coated with Organo-Selenium Inhibit Bacterial Attachment. Society for Biomaterials Meeting, April 22-25, 2009. San Antonio, TX. 16. Tran, P., Hammond, A., Mosley, T., Cortez, J., Gray, T., Colmer-Hamood, J., Looney, K., Shashtri, M., Spallholz, J., Hamood, A., and Reid, T. An Organo-Selenium Coating on Celllulose Inhibits Pseudomonas aeruginosa and Staphylococcus aureus Biofilms. The 20th annual TTUHSC Student Research Week, Texas Tech University Health Sciences Center. 2008. Lubbock, TX. 17. Zhou, X., Tran, P., Fralick, J. and Reid, T.W. Design and structure determination of a selenium labeled peptide antimicrobial. Protein Society, June 18-3, 2005. San Diego, CA. 18. Reid, T.W., Tran P., Spallholz J., and Fralick, J. The use of a selenium-peptide to specifically inactivate Yersinia pestis. 2003 Joint Service Scientific Conference on Chemical & Biological Defense Research, November 17-20, 2003. Hunt Valley MD. 19. Reid, T.W., Tran P., Spallholz J., and Fralick, J., Counter Terrorism Measures to Combat Yersinia pestis with Selenium. Annual Meeting of the Consortium of Biological Defense Researchers, April 2830, 2003. Lubbock, TX. 20. Reid, T.W., Tran P., and Fralick, J.A. Counter Terrorism Measures to Combat Yersinia pestis with Selenium. 2002 Joint Service Scientific Conference on Chemical & Biological Defense Research, November 19-21, 2002. Hunt Valley MD. 21. Reid, T.W., Tran, P., Nishida, T., and Murphy C. J., The Stimulation of Rabbit Corneal Epithelial Cell Migration in Culture by Substance P, is Synergistic with Insulin and IGF-1, ARVO, May 9-14, 1999. Ft. Lauderdale, Florida. 22. Tran, P. and Reid, T. 1998. The Effects of Substance P (SP), Insulin, and Insulin Like Growth Factor-1 (IGF-1) on Epithelial Cell Migration. The American Society for Cell Biology 38th Annual Meeting December 12-16, 1998. San Francisco, CA. 23. Tran, P. and Reid, T. 1998. The Effects of Substance P (SP), Insulin, and Insulin Like Growth Factor-1 (IGF-1) on Epithelial Cell Migration. The annual HHMI/TTU Student Research Week, Texas Tech University. 1998. Lubbock, TX. Hobbies and Interests 7 I enjoy reading non-fiction books, traveling, playing sport, caring for animal companions and socializing with friends and family. Referees Dr. Ted W. Reid, Ph.D. Address: Texas Tech University Health Sciences Center Department of Ophthalmology and Visual Sciences School of Medicine 3601 4th Street STOP 7217 Lubbock, TX 79430-7217 Tel: 806.743.2020, Ext. 262 Email: ted.reid@ttuhsc.edu Revised 4/15/2016 8 9