How clean is your drinking water? Microbiology of urban water systems: an interdisciplinary approach Peter Deines1,2,3, Mark Osborn2, Joby Boxall3 & Catherine Biggs1 1 Department of Chemical and Process Engineering, The University of Sheffield, UK 2 Department of Animal and Plant Sciences, The University of Sheffield, UK 3 Department of Civil and Structural Engineering, The University of Sheffield, UK P-161 LIF Why study drinking water distribution systems? The formation of microbial biofilms on pipe walls causes following problems: • public health problems - pathogens • aesthetic problems - undesirable tastes, odours, visual turbidity • major costs for water companies through microbial growth p.deines@sheffield.ac.uk Science objective: Design innovative and effective controls strategies that will ensure safe and highquality drinking water Experimental lab work Field studies 4 3 5 • isolation of bacterial strains from drinking water 7 1 • planktonic microbial community composition in natural systems QuickTime™ and a TIFF (Uncompressed) decompressor are needed to see this picture. P5-2 P2-1 P4-1 P1-1 P4-2 P5-1 • studying interspecies interactions in biofilm formation • water age and diversity Drinking water - an Ecosystem Water channel Matrix for the survival of pathogens Fast growing cells Slow growing cells Pathogens multicellular structures low-nutrient environment nutrient stress triggers aggregation planktonic cells biofilm Biofilm dispersal and detachment Conditions to be tested: • hydraulics • temperature • water quality Laboratory pipe test facility • community profiling of planktonic and biofilm microbial communities in response to changing conditions • studying biofilm characteristics References Figures taken from: Stoodley et al. 2002, Vreeburg et al. 2007, www.ehu.sbs.soton.ac.uk/art/biofilm (modified)