REPORT #1
Spectrophotometry
Name_______________________
Relationship between wavelength and color: Write down the wavelength of each color.
red___________
orange___________
yellow____________
green_________
blue_____________
violet_____________
% Transmittance and absorbance at (540 nm) for spectrophotometer calibration:
% transmittance_________________
absorbance_________________
Development of a Standard Curve
Using the absorbance of the different concentrations obtained in class; draw the
graph using the x-axis for the concentrations and the y-axis for the absorbance
readings.
Table of absorbance readings according to concentrations
0.625 mg/ml 1.25 mg/ml 2.5 mg/ml
5 mg/ml
10 mg/ml
unknown
Graph protein mg/ml (x-axis) vs. absorbance(y-axis)
Determine protein concentration (mg/ml) of the unknown. ____________ mg/ml.
REPORT #2
Acids, Bases and pH
Name___________________________
Making a set of Standards: Determine the colors of each pH.
pH
2
4
6
7
8
10
12
Colors
Determine the pH of unknown solutions comparing the colors to those of the standards.
A___________
B____________
C___________
Measuring pH using pH meter: Determine the pH of each solution.
Which of these are acids, basis or neutral solutions?
Solution
pH
Which of these solutions was the most acidic? ____________________________
Which of these solutions was the most basic? _____________________________
Determining the Buffering Capacity of a solution:
State your hypothesis about which of these solutions (A or B) will be a better buffer.
______________________________________________________________________________
Draw the graphs for both solution A and B using different colors and labeling them.
0
ml HCL added
ml of NaOH added
Which of the two buffers tested, A or B, has the highest buffering capacity?________________
State your conclusion regarding this last experiment ___________________________________
_____________________________________________________________________________
REPORT #3
Macromolecules
Name_____________________________
Chose 3 of the 6 solutions seen in class and fill in the information asked for below.
Solution 1:______________
Solution 2:______________
Solution 3:__________________
Write down the hypothesis about solution 1: __________________________________________
Write down the hypothesis about solution 2: __________________________________________
Write down the hypothesis about solution 3: __________________________________________
Color (+)
Color (-)
Solution 1
(+/-)
Solution 2
(+/-)
Solution 3
(+/-)
Reducing sugars
Starch
Protein
What is your conclusion about the contents of solution 1? ______________________________
____________________________________________________________________________
What is your conclusion about the contents of solution 2? ______________________________
_____________________________________________________________________________
What is your conclusion about the contents of solution 3? ______________________________
____________________________________________________________________________
Do you think there were any errors in your results?_______________________________
________________________________________________________________________
REPORT #4
Osmosis & Diffusion
Name___________________________
Effect of Molecular Size and Temperature
State your hypothesis regarding molecular size:
________________________________________________________________________
________________________________________________________________________
State your hypothesis regarding temperature:
________________________________________________________________________
Janus Green
(RT)
Janus Green
(37°C)
Potassium Permanganate
(RT)
Potassium
Permanganate
(37°C)
0 min.
30 min
What are your conclusions? _________________________________________________
Effect of solute concentration
State your hypothesis ______________________________________________________
Group
Contents of
Bag
Contents of
Beaker
0 minutes
40 minutes
Total Weight
Change (g)
1
2
3
What are your conclusions regarding this experiment? ___________________________
Selective Permeability of Membranes
State your hypothesis ______________________________________________________
Test tube #
Solution present in beaker
1
2
3
4
5
mixture
mixture
mixture
mixture
mixture
Ion/Molecule
tested for
Result (+/-)
State your conclusions _____________________________________________________
Osmosis in Red Blood Cells: Exposure to different environments
Hypothesis about tube A
Hypothesis about tube B
Hypothesis about tube C
________________________________________________________________________
Tube
Solution
A
0.9 NaCl
B
10% NaCl
C
0.45% NaCl
Tonicity
Appearance of cells
(draw)
What can you conclude about the effect of tonicity on animal cells? _________________
________________________________________________________________________
REPORT #5
Enzymes
Name_________________________
Write down your hypothesis in regards to the temperature effect on enzyme activity
________________________________________________________________________
Data table for temperature effect on enzyme activity
Temperature °C
Glucose mg/dL
0
RT
40
60
80
100
Conclusions: If they don’t coincide with the hypothesis, explain why.
________________________________________________________________________
Graph glucose on the y-axis vs temperature on the x-axis
Temperature
Write down your conclusions of this experiment ________________________________
_______________________________________________________________________
Is the graph any different from the theoretical result you should have obtained? If so,
explain. _________________________________________________________________
Write down your hypothesis in regards to the effect of pH on enzymatic activity_______
Data table for the pH effect on enzyme activity:
pH
[Glucose]
2
4
6
7
8
10
12
Graph glucose concentration on the y-axis and pH on the x-axis
pH
What are your conclusions in regards to this experiment? Are these any different from the
theory?._________________________________________________________________
Specificity
Write down your hypothesis regarding enzyme specificity:_________________________
________________________________________________________________________
________________________________________________________________________
Results
Substrate
Lactose
Maltose
Glucose production (mg/dL)
Conclusions:_____________________________________________________________
________________________________________________________________________
________________________________________________________________________
Cofactor
Write down your hypothesis regarding this experiment ___________________________
Results
Control
EDTA
Write down your conclusions _______________________________________________
_______________________________________________________________________
REPORT #6
Cell Respiration
Name ___________________________
Oxygen consumption as a measurement of metabolic rate and its relation to the size
of the animal in study:
Hypothesis_________________________________________________________
__________________________________________________________________
__________________________________________________________________
Mouse's weight (g) ______
Oxygen consumption data sheet
____________________________________________________________________
Trial # Vol O2
Time
Time interval
ml O2/h
ml O2/g/h
used
(minutes)
(hours)
1___________________________________________________________________
2___________________________________________________________________
3___________________________________________________________________
4___________________________________________________________________
5___________________________________________________________________
Average ____________
Conclusion ___________________________________________________________
____________________________________________________________________
____________________________________________________________________
Aerobic Respiration: CO2 production in plant embryos
Hypothesis ______________________________________________________________
Indicator color
Results
Germinated
Germinated-boiled
CO2 presence (+/-)
Conclusion _____________________________________________________________
Fermentation in yeast
Hypothesis ______________________________________________________________
Results
Time (min)
0
10
20
30
Glucose 0%
Glucose 1%
Starch 1%
Conclusion ______________________________________________________________
REPORT #7
Photosynthesis
Name_________________________
Absorption Spectrum
Hypothesis ______________________________________________________________
A
S
C
400
425
450
WAVELENGTH
475 500 525 550
575
600
625
650
675
700
Absorption Spectrum Graph: Draw the graphs of both spinach and carrot extracts. Use
green ink or pencil for spinach and red ink or pencil for carotene. Use absorption in the yaxis vs wavelength in the x-axis.
Conclusion _____________________________________________________________
Pigment Chromatography
Hypothesis ______________________________________________________________
Draw the representation of the chromatography
pigment separation.
Conclusions _____________________________________________________________
Starch localization in leaves
Draw the 2 leaves treated with ethanol and exposed to iodine.
Explain the difference in color between the two leaves.