David Snow Photosystem 2 1. noncyclic 2. occurs/resides in thylakoid membrane 3. reaction center chlorophyll is P680 Photosystem 1 1. can be cyclic, if required 2. occurs/resides in thylakoid membrane 3. reaction center chlorophyll is p700 4. passes photoexcited electrons to photosystem 1 via an electron transport chain 5. Light enters chlorophyll and excites an electron. The energy from this electron is passed from electron to electron until an electron in the reaction center is excited and physically removed to the primary acceptor 4. uses ‘spent’ electrons from photosystem 2 to make up the number of electrons in its own system 5. ‘Passes’ spent electrons back to prior electron transport chain to create extra ATP if needed (this is the cyclic process). 6. This excited electron is passed down the electron transport chain to photosystem 1 6. Passes its own photo excited electrons to an electron transport chain, which transmits them to Fd, an iron-containing protein 7. NADP+ reductase transfers the electrons from Fd to NADP+ creating NADPH 7. Splits water into oxygen and electrons. Water is the source of electrons for this process 8. ATP produced during noncyclic electron flow is called noncyclic photophosphorylate 9. 8. This NADPH provides reducing power for the synthesis of sugar in the calvin cycle 9. Calvin Cycle 1. occurs in stroma 2. CO2 enters and leaves as sugar 3. cycle spends ATP as energy and consumes NADPH as reducing power for adding high-energy electrons to make sugar 4. returns NADP+ to the light reactions 5. 3 phases: carbon fixation: each CO2 is fixed into a 5-carbon sugar, using RuBP reduction: each 3-phosphoglycerate receives a phosphate from ATP. A pair of electrons from NADPH then reduces it to G3P regeneration: RuBP is regenerated, so that CO2 can again be accepted, and cycle can turn again 6. G3P is the product of this cycle, not glucose. G3P is a sugar 7. Cycle must turn 3 times, fixing 3 carbons, to create one net G3P 8. For net synthesis of one G3P, the cycle consumes 9 ATP and 6 NADPH, these molecules are regenerated by the light reactions 9. G3P is starting material for metabolic pathways that synthesize other organic compounds