Chapter 8 Photosynthesis 8-1 Energy and Life 8-1 Energy and Life 8-1 Energy and Life Autotrophs • Make their own food 8-1 Energy and Life Heterotrophs • Cannot make their own food 8-1 Energy and Life Chemical Energy and ATP • The principal chemical compounds that cells use to store and release energy is called ATP adenosine triphosphate 8-1 Energy and Life Storing Energy • ATP stores energy in the third phosphate • ATP is like a fully charged battery 8-1 Energy and Life Releasing Energy Q: How is the energy in ATP released? A: Break bond between the second and 3rd phosphates 2 ADP 8-1 Energy and Life 8-1 Energy and Life Using Biomechanical Energy • Active Transport • Movement of organelles through cell • Sythesis of proteins and nucleic acids • Produce light – Blink of firefly caused by an enzyme powered by ATP 8-1 Energy and Life ATP Availability • Most cells only have enough ATP for a few seconds of activity • Why? – Not good at storing energy over the long term – Glucose stores 90X the chemical energy of ATP – Cells generate ATP from ADP as needed by using the energy in foods like glucose 8-2 Photosynthesis: an Overview 8-2 Photosynthesis an Overview During Photosynthesis… • Organisms (plants) absorb light energy from the sun and store it in organic compounds. • Energy is crucial to all life, without it work could not be done. 8-2 Photosynthesis an Overview AN OVERVIEW OF PHOTOSYNTHESIS Photosynthesis is the process by which autotrophic organisms use light energy to make sugar and oxygen gas from carbon dioxide and water 8-2 Photosynthesis an Overview An example of basic photosynthesis… 8-2 Photosynthesis an Overview The Photosynthesis Equation CO2 + H2O (Carbon Dioxide) (Water) light (Sun) C6H12O6 + O2 (Glucose) (Oxygen) 8-2 Photosynthesis an Overview Because light is a form of energy… • Anything that absorbs light also absorbs the energy from that light • When chlorophyll absorbs light, much of the energy is transferred to electrons in the chlorophyll molecule, raising the energy level of these electrons • These high-energy electrons make photosynthesis work 8-2 Photosynthesis an Overview Photosynthesis 8-2 Photosynthesis an Overview Chloroplasts • The site of Light Dependent Reactions • These organelles are surrounded by a double membrane and contain an inner membrane separate into disk like sacs called thylakoids • Thylakoid are arranged into granum, or neat stacks • Each thylakoid contains the green pigment chlorophyll • The light absorbing pigments are organized into photosystems, which transfer energy during the light reactions 8-2 Photosynthesis an Overview 8-2 Photosynthesis an Overview Pigments in chloroplasts • Chloroplasts absorb all other color pigments, leaving green to be reflected resulting in a plant’s color. • Chlorophyll a and b are two are the 2 most common types of chlorophyll Light • Reflected light Chlorophyll b absorbs colors or light energy NOT absorbed by chlorophyll a Absorbed light Transmitted light Chloroplast 8-2 Photosynthesis an Overview Absorption of Light by Chlorophyll a and Chlorophyll b Chlorophyll b Chlorophyll a V B G YO R 8-3 The Reactions of Photosynthesis 8-3 Reactions of Photosynthesis 8-3 Reactions of Photosynthesis Thylakoids • Sac-like photosynthetic membranes arranged in stacks 8-3 Reactions of Photosynthesis Grana • Stacks of thylakoids 8-3 Reactions of Photosynthesis Stroma • The region outside the thylakoid 8-3 Reactions of Photosynthesis Scientists describe the reactions of photosynthesis in two parts 1. Light – dependent reactions (in the thylakoid membranes) 2. Light – independent reactions (takes place in stroma) 8-3 Reactions of Photosynthesis 8-3 Reactions of Photosynthesis Photosynthesis Consists of 2 Stages • Light dependent reactions • Non-light dependent (dark) reactions • Take place in the Thylakoid Membrane • Takes place in the Stroma • Depends on sunlight for activation energy • Includes the Calvin cycle • Responsible for the absorbing of light in Photosynthesis • Does not literally occur in the dark, but requires no light to occur • Water is split, • giving off oxygen • Carbon dioxide is split, • Carbon to make sugars • H2O + O2 + ATP + NADPH2 • ATP + NADPH2 + CO2 + C6H12O6 8-3 Reactions of Photosynthesis 8-3 Reactions of Photosynthesis Carrier Molecule • Compound that can accept a pair of high energy electrons and transfer them along with most of their energy to another molecule • Ex.) NADP+ 8-3 Reactions of Photosynthesis 8-3 Reactions of Photosynthesis Q: What does this do? • NADP + H NADPH A: this traps sunlight in chemical form 8-3 Reactions of Photosynthesis Light Dependent Reactions • Uses energy from light to produce 1. Oxygen gas 2. ATP 3. NADPH 8-3 Reactions of Photosynthesis Watch The Light Reaction Video 8-3 Reactions of Photosynthesis 8-3 Reactions of Photosynthesis Watch The Light Reaction Video 8-3 Reactions of Photosynthesis The Calvin Cycle • The ATP and NADPH formed by the lightdependent reactions contain an abundance of chemical energy, but they are not stable enough to store that energy for more than a few minutes. • The Calvin cycle uses ATP and NADPH from light – dependent reactions to produce high energy sugars 8-3 Reactions of Photosynthesis The Calvin Cycle • These reactions don’t require light, therefore these reactions are called • Light – independent reactions 8-3 Reactions of Photosynthesis Watch The Calvin Cycle Video 8-3 Reactions of Photosynthesis Watch The Calvin Cycle Video 8-3 Reactions of Photosynthesis Factors Affecting Photosynthesis • Not enough water • Temperature • Light intensity 8-3 Reactions of Photosynthesis Review: Photosynthesis uses light energy to make food molecules Chloroplast Light Stroma NADP Stack of thylakoids ADP +P Light reactions Calvin cycle Sugar used for Cellular respiration Cellulose Starch Other organic compounds 8-3 Reactions of Photosynthesis