Cryptomonadales®: a unique species of Chlorella
sorokiniana developed by MR. Shun-Te Wang
Nutritional Value of the ChlorellaCryptomonadale ® Product:
Perfect health food for 21st century
The first certificate of algae health food in Taiwan
Lack cell wall, easily and almost fully ( 90% )
absorbed
Perfect nutritional composition:
Protein : CHO : lipid =60:14:8
Optimal combination of fatty acid:
saturated FA: mono-unsaturated: polyunsaturated =
0.8:1.5:1.0
Chlorophyll: 4%
Vitamin A, B, C, E
Minerals
Trace elements: Zinc ( 70 mg% ), Selenium: 80mg%
Reports on the effects of Cryptomonadales
on medicines:引藻的醫療研究報告

Crypto- can reduce the levels of cholesterol and lowdensity lipoprotein（LDL）for up to 15-20% in one month

Regulation of blood glucose and glycosylated Hgb to
nearly normal in 3-6 months

Crypto- can modulate immune function ( contact dermatitis,
psoriasis, rheumatoid arthritis, and wound healing )。

Crypto- contains phycocyanin and has anti-viral effects

Potential prophylactic effects for cancers。
引藻可降低自願受測者血中膽固醇
A. Cholesterol
cholesterol (mg/ml)
215
210
205
200
195
190
185
180
175
170
165
before
2 weeks
4 weeks
after
**資料來自國衛院 蘇益仁主任
PPARs ( peroxisome Proliferator Activated
Receptor ): the most promising molecules for
drug development in 21th century
Peroxisome proliferatoractivated receptor pathway
diagram
From Wikipedia, the free encyclopedia
Biomedical effects of PPAR agonists:
Fatty acid is the natural ligand for PPARs
Effects of PPAR agonist on metabolic syndrome
( diabetes, obesity, hyperlipidemia, and hypertension )
**資料改編自Science 2003;302:406
PPAR Agonist Activities in ChlorellaCryptomonadales® and Its
Biomedical Application
Ih-Jen Su, MD, PhD
Division of Clinical Research,
National Health Research Institutes,
Taiwan
Extraction of PPARs-containing Fraction
from Cryptomonades
**資料來自國衛院
生技製藥組
Effects of Crypto- onPPARs expression
Control
Fenofibrate
GLA
EPA
cryp.
tablet
＊Fenofibrate： 50 μg/ml
Tubulin
(as PPARα control)
＊GLA：γ-Linolenic Acid
PPARα
＊EPA：Icosapentaenoic acid
＊cryp. tablet：引藻片
＊引藻處理濃度：2 mg/ml
3.5
3
2.5
2
1.5
1
0.5
0
數列1
C ontr ol
F enofibr ate
G LA
EPA
c r y p. tablet
1
1.25
1.21
1.19
2.87
Pilot trial on psoriatic arthritis
曾接受數種治療方式但未獲改善之患者,服用引藻10周以上評估其關節炎狀況
°®Å~©ÊÃö¸`ª¢¯kµh«ü¼Æ
40
Before
1 month
2 month
3 month
30
Pain score
PASI score
10.0
20
10
7.5
5.0
2.5
0
0
2
4
6
8 10 12 14 16 18 20 22 24
Time (week)

0.0
Before
1 month
2 month
3 month
Time after treatment
Improvement of arthritis and skin lesions
**資料來自成大醫學院皮膚科 李玉雲醫師,林旻憲醫師
Crypto-PPAR induce suppression of
cytokine tumor necrosis factor-alpha
Immune mdulation and anti-inflammatory effects
300
Control
TNF (pg/ml)
TPA 2.5ng/m l
TPA+Troglitazone
200
TPA+Cryp 0.25
TPA+Cryp 1
100
0
0
10
20
30
40
50
Time (hour)
TP A ³B²z24¤p® É«á ¦A¥[¤JTroglita zone ,Cryp.
60
Crypto-PPARs can effectively reduce
viral load in animal model
60000
Viral load (copies/ml)
PBS 64403
PBS 54409
50000
HVP 59603
40000
HVP 66007
Cryp 8d + HVP 59901
30000
Cryp 8d + HVP 60503
Cryp 8d + HVP 55301
20000
HVP + Cryp 7d 59501
HVP + Cryp 7d 66503
10000
HVP + Cryp 7d 60501
0
d0
d6
d12 d17 d20 d24 d27 d31 d34 d38
Time (day)
Crypto-PPAR can reduce the oxidative
stress and genomic instability:
anti-aging and cosmetics
53KD
PPAR α
54.5KD
57.6KD
PPAR γ
GRP94
94KD
125KD
40KD
30KD
110KD
54KD
46KD
PERK
eIF2
GADD153
IRE1
P-JNK
JNK
PPARs agonists: the most promising drugs;
Chlorella-Cryptomonadales the best natural
product in the 21st century

Metabolic syndrome: hyperlipidemia、
diabetes、hypertension、obesity

Immune diseases: arhtritis, eczema, allergy

viral infection: influenza, enterovirus,
? HIV/AIDS , ? hepatitis

Aging: oxidative stress, cosmetics

Cancer prevention and therapy
Adipocyte Differentiation Assay
For PPAR-gamma ligand
**資料來自國衛院生技製藥組
100
200
80
150
100
%
%
60
50
40
20
0
0
CE 3-1 CE 3-2 CE 3-3 CE 3-4 CE 3-5 CE 3-6
CE 1 CE 2 CE 3 CE 4 CE 5 CE 6 CE 7 CE 8
80
CE 1
CE 2
CE 3
CE 4
CE 5
CE 6
CE 7
CE 8
60
40
% of maximum
% of maximum
80
20
0
100
101
g/ml
102
60
CE 3-1
CE 3-2
CE 3-3
CE 3-4
CE 3-5
CE 3-6
40
20
0
100
101
102
g/ml
**資料來自國衛院生技製藥組
Extracts of Cryptomonadales contain high
concentration of allophycocyanin: effective
for anti-viral
Molecular mechanism of immune modulation
and anti-inflammation by PPARs
Nature Rev Immunol. 2002; 2:748-759
Index molecule in Cryptoand quality assurance
引藻片的品管檢驗



次亞麻油酸(Linolenic Acid): 18mg/gm
Free of bacteria and safety levels of heavy metals
Stability in 3 months at room temperature
**資料來自工研院