LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2011
BT 2820 - STRUCTURAL BIOLOGY & BIOINFORMATICS
Date : 11-04-2011
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
Section A
Answer all questions
I Choose the correct answer
(20marks)
(5 x 1 = 5)
1. The term nucleic acids is the generic name for all _____________.
a) proteins
b) biopolymers
c) monomers
d) dimers
2. A coiled coil has ____________ alpha helices coiled together.
a) 1-6
b) 2-7
c) 3-8
d) 4-9
3. mutation in NLRP3 gene leads to ____________ syndrome.
a) PATS
b) MIPS
c) CAPS
d) SCD
4. _____________ carries out work on mouse models at EMBL.
a) Heidelberg
b) Hinxton
c) Grenoble
d) Monterotondo
5. Oligosaccharide structures that occur in proteins are predicted by _____________.
a) GlycoMod
b) GlycoPept
c) PeptMAP
d) FindMod
II Say whether the following statements are True or False; if false give reason
(5 x 1 = 5)
6. During replication the leading strand is formed by DNA polymerase (III).
7. Screw rotation is a symmetrical form of tertiary structures.
8. Ia is weak helix breakers.
9. Nucleotide sequences for DDBJ can be submitted through software called SAKURA.
10. Axeldb database contains genomic expression of Xenopus sp.
.
III Complete the following
(5 x 1 = 5)
11. Polynucleotides with very high content of guanine form ______________.
12. The total number of ways of arranging an object to form a 3D lattice is _____________.
13. _____________ developed the levinthal paradox in 1969.
14. Computational tool for iterative methods is_____________.
15. _____________ is a soft ionization technique.
IV Answer the following, each in about 50 words
16. Define stereomers and rotamers.
17. What are super secondary structures?
18. Explain the rational design strategy used in protein engineering.
19. Define CSD.
20. Differentiate between orthologs and paralogs.
(5 x 1 = 5)
Section B
V Answer the following, each answer within 500 words, draw diagrams wherever necessary
(5 x 8 = 40)
21. (a) Briefly explain about the alpha helices and beta sheets in protein secondary structures.
OR
(b)Write notes on topoisomerases and supercoiling.
22. (a) Explain the fibrillar structure, properties and uses of collagen.
OR
(b) Briefly summarize the molecular pathology of hemoglobin abnormalities.
23. (a) Explain levinthal paradox and protein folding pathways.
OR
(b) Summarize protein structure prediction by NMR technique. Add a note on protein
engineering.
24. (a) Explain the protein sequence databases.
OR
(b) Explain a palindromic sequence with a suitable diagram.
25. (a) What are the types, goals of the HGP. List out the other completed genome projects.
OR
(b)Give an account on the SNP types and genotyping.
Section C
VI Answer any two of the following, each within 1500 words; draw diagrams wherever
necessary
(2 x 20 = 40)
26. Account on Ramachandran plot and on the structure of RNA.
27. What is hemoglobin? Explain the oxygen binding, Bohr effect and molecular basis of
sickle cell anemia.
28. Define phylogenetics. Draw an evolutionary tree to illustrate it. Draw a dot matrix for
Sequence1: GATCTATT
Sequence2: GATCATC
29. Give a detailed account on the Mass spectroscopic technique to identify unknown proteins.
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