LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2011
BT 2819 - ENVIRONMENTAL BIOTECHNOLOGY
Date : 12-04-2011
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
Section A
Answer all questions
I Choose the correct answer
(20marks)
(5 x 1 = 5)
1. Match the following microbes with the terms associatated with them:
A) Methanobacterium B) Cellulomonas C) Thiobacillus D) Azotobacter
i) sulphur oxidizer ii) gobar gas
iii) nitrogen fixation iv) cell wall lysis
a)
b)
c)
d)
A – ii,
A – ii,
A – ii,
A – ii,
B – iii,
B – i,
B – iv,
B – iii,
C – i,
C – iii,
C – i,
C – iv,
D – iv
D – iv
D – iii
D–i
2. OCT plasmid degrades
a) octane, hexane, toluene
c) xylene, hexane, naphthalene
b) toluene, naphthalene, octane
d) octane, hexane, decane
3. In sugar industry, partially crystallized syrup from the vacuum pan is known as
a) massecuite
b) black strap molasses
c) mother liquor
d) bagasses
4. _____________ has the natural ability to dechlorinate tri,-tetra-& penta-chlorodioxins.
a) Dehalococcoides
b) Desulfitobacterium
c) Deinococcus
d) Dehalobacter
5. The recirculation ratio R is
a) R = Q/QR
b) R = 0
c) R = QR/R
d) R = RQ/R
II Say whether the following statements are True or False; if false give reason
(5 x 1 = 5)
6. In thermally stratified lakes, the hypolimnion is often aerobic.
7. Emulsification increases the surface area between two liquids.
8. Pseudomonas aeruginosa is employed in mining low grade ore. .
9. RBC works on the principle of anaerobic attached growth system.
10. DGGE is Decreasing Gradient Gel Electrophoresis.
III Complete the following
(5 x 1 = 5)
11. The causal organism of airborne disease diphtheria is __________ __________.
12. Final clarifier is otherwise called as _________________.
13. Oils with high proportion of low molecular weight material are known as _____________.
14. The decomposition of heavy petroleum distillates into lighter product is called __________.
15. Any detectable changes in the host cell due to viral infection are termed as ____________.
IV Answer in one or two sentences, each in about 50 words
(5 x 1 = 5)
16. What is ANOVA?
17. Write the overview of waste water treatment.
18. What is aerated lagoon?
19. How are pesticides classified?
20. Mention any three applications of PCR.
Section B
V Answer the following, each answer within 500 words, draw diagrams wherever necessary
(5 x 8 = 40)
21. (a) Write about the microbes present in the extreme environments.
OR
(b) Discuss the effects of nuclear waste pollution. Add a note on the recent happenings in Japan related
to this aspect.
22. (a) Explain the different stages involved in biofilm formation.
OR
(b) Briefly explain the different steps involved in pre-treatment of source water.
23. (a) Illustrate the use of GEMs in bioremediation
OR
(b) Write notes on:
(i) Bioremediation of Xenobiotics
(ii) Bioremediation of heavy metals
24. (a) Explain the steps involved in degradation pattern of pesticides.
OR
(b) Describe the production process in sugar industry.
25. (a) Give a brief account on methane production from wastes.
OR
(b) Explain the detection of bacteriophages & plant viruses from the soil sample.
Section C
VI Answer any two of the following, each within 1500 words; draw diagrams wherever necessary
(2 x 20 = 40)
26. Briefly explain the process involved in trickling filters for attached microbial growth &
biofilm kinetics with advantages & disadvantages.
27. Distinguish between autecology and synecology. Write about the salient aspects of food
chain and food web.
28. Explain the commonly used molecular markers for environmental monitoring of
microbes.
29. Write notes on:
i) Leather processing
ii) Effluent treatment in Tannery industry
iii) Microbial applications to Tannery industry
iv) Treatment of refinery waste water
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