M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
THIRD SEMESTER – NOVEMBER 2012
BT 3816 - ANIMAL CELL BIOTECHNOLOGY
Date : 01/11/2012 Dept. No.
Time : 9:00 - 12:00
Max. : 100 Marks
PART – A (20 marks)
Answer all the questions
1. Choose the correct answer: (5 x 1 = 5 marks)
(1) Which one of the following types of laminar flowhood is most suitable for animal
cell culture?
(a) horizontal (b) vertical (c) diagonal (d) vented
(2) Serum cytotoxicity is due to
(a) properdin (b) polyamine oxidase (c) proteolysin (d) cytolysin
(3) A polymer that is widely used for tissue engineering is
(a) polylactic acid (b) polyacrylic amide (c) polyester (d) polylysine
(4) A double stranded RNA virus is known as a
(a) herpes virus (b) adenovirus (c) retrovirus (d) reovirus
(5) Cloning of animals can be done by fusing a nucleated somatic cell with an enucleated
(a) oocyte (b) sperm (c) embryo (d) blastocyst
II. State whether the following are true or false; if false give reason: (5 x 1= 5 marks)
(6) Transformed cells express high levels of fibronectin.
(7) Polyethylene glycerol is a cryoprotectant.
(8) Cord blood stem cells can transdifferentiate.
(9) Rubella viruses are propagated on chinese hamster ovary cells for vaccine production.
(10) Prostaglandins can be used for oestrous synchronization in cattle.
III. Complete the following: (5 x 1 = 5 marks)
(11) The split ratio or dilution at subculture should ensure that the cells are not in the ________ phase for long.
(12) When ________ buffer is used in culture media, carbondioxide is not required to maintain the pH.
(13) Recombinant tissue plasminogen activator was produced in ________ cells by Genentech.
(14) Hepatitis A vaccine is propagated on ________ cells.
(15) ________ is done by administering follicle stimulating hormone and leutinizing hormone from day 2 or 3 through day 8 to 12 of the menstrual cycle.
IV. Answer the following, each within 50 words only: (5 x 1 = 5marks)

(16) Why does a drop in pH of the medium indicate the need for feeding?
(17) How does isozyme analysis help in characterizing a cell line?
(18) What is meant by commitment of a cell during differentiation?
(19) Which virus is propagated by yolk sac inoculation of chick embryos?
(20) Give two examples of embryo coculture cells.
PART – B (5×8 = 40 marks)
Answer the following questions, each in about 500 words only. Draw diagrams wherever necessary:
21 (a) Explain the tests for mycoplasma contamination.
OR
(b) Describe the aseptic techniques to be followed while performing animal cell culture.
22 (a) What are the steps in the preparation of the cell sample for cryopreservation?
OR
(b) Discuss the following:
(i) Balanced salt solutions
(ii) Any four advantages of using serum during cell culture
23 (a) Write a note on the different types of tumorigenesis assays.
OR
(b) Explain the method and applications of spheroid culture.
24 (a) Discuss how the safe transportation of viruses can be achieved.
OR
(b) Write a note on viral taxonomic classification.
25 (a) Describe how the polymerase chain reaction is used for the diagnosis of sickle cell anemia.
OR
(b) Explain how embryo grading is done.
PART – C
(2×20 = 40 marks)
Answer any TWO of the following questions, each in about 1500 words; Draw diagrams wherever necessary:
(26) Discuss the following:
(i) Characteristics of cells in culture (12)
(ii) General characteristics of viruses (8)
(27) Write notes on:
(i) Embryonic stem cells (12)
(ii) Promoters and markers used in animal cell expression system (8)
(28) Give an account of the different methods of production of transgenic mice and their applications.
(29) Explain the different methods of organ culture.
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