LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
THIRD SEMESTER – NOV 2006
AE 21
BT 3875 - ANIMAL BIOTECHNOLOGY
Date & Time : 06-11-2006/9.00-12.00 Dept. No.
Max. : 100 Marks
PART-A
I. Choose the correct answer:
(5x1=5)
1.These cells can grow continuously in invitro conditions
a) neurones
b). Cancer cells
c) RBC
(d) none.
2. ELISA is used to diagnose
a). HIV
b). Hay fever
c). anemia
(d) diabetes
3. Provides the suitable environmental conditions to the growing animal cells in invitro
conditions
(a) CO2 incubator
(b) O2 incubator
(c) N2 incubator
(d) all of the above.
4. This is from thermos aquatics
a) RNA polymerase b)Taq polymerase c) Rag proteins
(d) oligonucleotides.
5. Number of amino acids present in β chain of insulin
a) 20
b) 30 c) 40
(d) 50.
II. State true or false; if false give reasons:
6. High serum cultures are more susceptible to fluid mechanical damage.
7. He La cells isolated and cultured from human cervical carcinoma
8. Finite cell lines grow a limited number of cell generation.
9. The size of the human genome approx. 3,200 million bases.
10. FISH test is called as Fluorescence insitu hybridization
(5x1=5)
III Complete the following
(5x1=5)
11. Malarial parasite ________________identified by DNA probe.
12. Plasmogen converted to plasmin by________________
13. H chain and L chain of immunoglobulin molecules cloned into_______________
vectors.
14. _______________is a growth hormone produced as recombinant proteins.
15. ________________ gene is the largest known human gene with 2.4 million bases.
IV. Answer the following questions, each in about 50 words:
16.
17.
18.
19.
20.
Define DNA finger printing
Explain transgenic fishes
What are expression vectors?
Write any two types of PCR methods.
List the different methods of transfection.
(5x1=5)
PART-B
V. Answer any FIVE of the following question, each in about 350 words
(5 x 8 =40)
21. Write a short notes on primary and continuous cell lines
22. Explain the three types of unit process systems.
23. Discuss the characteristics of transformed cells
24. Write a short note on biolistics
25. Describe the production of recombinant insulin
26. How can you establish transgenic mice with retroviral vectors?
27. Explain nuclear transfer method to obtain transgenic animals
28. Write any two applications of micro array technique.
PART C
VI. Answer any TWO following questions, each in about 1500 words.
(2 x 20 =40)
29. (a) Explain Oligo nucleotide ligation assay-OLA/PCR and detection of single base mutation with
fluorescence labeled PCR primers.
(Or)
(b) Write in detail about production and application of transgenic animals with examples
30. ( c ). Explain how the cells are transformed by DNA and RNA oncogenic viruses
(Or)
(d). Write an essay on stem cell culture and its application
___________________________