LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2012
BT 2954 - MICROBIAL & BIOPROCESS TECHNOLOGY
Date : 26-04-2012
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART-A (20marks)
Answer all the questions
I Choose the correct answer:
(5 x 1 = 5 marks)
1. Thermophiles are microbes which can resist temperatures in the range of
a) -3 to 15oC
b) 0 to 25 oC
c) -15 to 0 oC
d) 40 to 70 oC
2. Which of the following is a chelating agent?
a) DDT
b) DMSO
c) EDTA
3. Flat panel configuration is an example of ____________ bioreactor.
a) Photo
b) Stirred tank
c) Packed bed
d) MMS
d) Fluidized bed
4. ____________ uses hydrostatic pressure to force the liquid against semipermeable membrane.
a) Dialysis
b) Microfiltration
c) Ultrafiltration
d) Nanofiltration
5. Presence of _____________ in drinking water causes blue baby syndrome in children.
a) Nitrite
b) Nitrate
c) Ammonia
d) Sulphur
II State whether the following statements are True or False; if false give reason:
(5 x 1 = 5 marks)
6. Down stream processing involves separation and purification of metabolites.
7. Fed Batch model is a combination of batch and continuous models.
8. Process monitoring is an application of computers in the field of bioprocess technology.
9. Tempered chrome steel is a variety of quartz.
10. S.uvarum is also termed as top fermenting yeast.
III Complete the following:
(5 x 1 = 5 marks)
11. During World War II, glycerol was used in large quantities for production of ____________.
12. In batch culture, the log phase is followed by _________________________.
13. ______________ is used as an antifoam chemical.
14. ______________ consists of silicaceous skeletal remains of tiny aquatic plants.
15. Pure form of ______________ is used in plastic industry.
IV Answer the following, each in about 50 words.
(5 x 1 = 5 marks)
16. Beer production is said to be “protected”-State a reason.
17. Why do some microbes show a diauxic growth pattern?
18. Define aspect ratio.
19. What is microfiltration?
20. What is Patenting?
PART-B
Answer the following, each in about 500 words. Draw diagrams wherever necessary.
(5 x 8 = 40 marks)
21. (a) Evaluate the different fermentation process on
i. Microbial enzymes ii. Recombinant products iii. Microbial biomass iv. Transformation
OR
(b) Describe any four physical factors in the environment that influences microbial growth.
22. (a) Discuss any four inoculum development methods.
OR
(b) Formulate a media suitable for the growth of microbes and optimal product formation.
23. (a) Analyse any four valves and its usage in fermenter.
OR
(b) Give an account on the design of a stirred tank bioreactor.
24. (a) Briefly explain physical and chemical methods of cell disruption .
OR
(b) What is liquid-liquid extraction?
25. (a) Explain the production of single cell proteins from bacteria and algae.
OR
(b) Give an account on the production of wine.
PART-C
Answer any TWO of the following, each in about 1500 words. Draw diagrams wherever
necessary.
(2 x 20 = 40 marks)
26. Elaborate on the various sterilization processes involved in fermentation.
27. Describe isolation and preservation of microbes for fermentation.
28. What is a bioreactor? Explain the different types of bioreactor.
29. Differentiate between batch and continuous filtration.
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