B.Sc. DEGREE EXAMINATION – ADVANCED ZOOLOGY & BIOTECH.
FOURTH SEMESTER – APRIL 2008
Date : 24/04/2008
Time : 9:00 - 12:00
PB 4206 / 4202 - MICROBIAL BIOTECHNOLOGY
Dept. No.
KL 12
Max. : 100 Marks
PART A (20 marks)
Answer All questions
I Choose the Correct Answer
01. Preservation of microorganism is by ………
( 5 x 1 = 5 marks) a. fermentation b. flocculation c. lyophilization d. refrigeration
02. Which of the following one is the substrate used for cheese production? a. Butter milk b. Milk c. Whey
03. ………. is the starting material for production of steroids? a. Diosgenin b. Streptomycin
d. Milk powder c. Cephalosporin d. Penicillin
04. Pick the odd one out a. Ascorbic acid b. Citric acid c. Acetic acid d. Glutamic acid
05. ……….. is used as a food additive for the preparation of soft foods. a. Renin b. Xanthan c. Lipids d. Biopol
II State whether the following statements are True or False (5 x 1 = 5 marks)
06. Intracellular metabolites are located outside the cells.
07. Immobilized cells can be used for biotransformation reactions.
08. Commercial production of enzymes is mostly from microbes.
09. Yoghurt is the fermented product of whole milk.
10. Neem oil is used as biofertilizer.
III. Complete the following ( 5 x 1 = 5 marks)
11. ………….. is used for the production of secondary metabolites.
12. Addition of ………. in wort enhances flavor and bitter taste in beer.
13. The most commonly used food preservative is ……..
14. The process in which microbes convert organic compounds into structurally relative
product is called …………
15. …………. is associated with root nodules of pulses.
IV. Answer all, each in about 50 words
16. Define Bioreactor
17. What is single cell protein?
18. Mention any one microorganism used in acetic acid production.
19. Define antibiotic.
20. Mention the importance of Azolla in Agriculture
( 5 x 1 = 5 marks)
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PART B ( 5 x8 = 40 marks)
Answer any five of the following, each within 350 words only. Draw diagrams and flowcharts wherever necessary.
21. Give a brief account of Downstream processing.
22. Define microbial technology. Briefly explain the operation of a fermentor.
23. Explain the steps involved in cheese production.
24. Describe the biosynthetic pathway of citric acid.
25. Explain the production methodology of Glutamic acid.
26. Give an account of biosynthesis of Penicillin.
27. Explain the production methodology of Riboflavin.
28. Write notes on Biopesticides.
PART C (2x 20 = 40 marks)
Answer the following, each within 1500 words only. Draw diagrams and flowcharts wherever necessary.
29 a. Explain in detail about the fermentation process. Add a note on carbon and nitrogen substrate used in fermentation industry. or
b. Describe the methodology of mass cultivation of Spirulina and mushroom. Add a
note on its nutritive value.
30 a. i). What is biotransformation? Explain the process of biotransformation of steroids.
ii). Define Biosensor. Explain the working principle and applications of biosensor.
or
b. i) Define Enzyme technology. Explain the production methodology of protease.
ii) Write notes on Bioplastics.
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