LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
FOURTH SEMESTER – APRIL 2007
IB 02
BT 4951 - METABOLIC ENGINEERING
Date & Time: 04/05/2007 / 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART - A
ANSWER ALL THE QUESTIONS.
I. Choose the correct answer:
(5 x 1 = 5)
1. Protein engineering of AAT (alpha 1 antitrypsin) was done by
a) site directed mutagenesis (b) gene shuffling (c) SCOPE method
(d) RACHITT method
2. Term used for describing the complement of mRNA transcribed in a cell is
(a) Transcriptome (b) proteome (c) genome (d) genomics
3. Soybean with enhanced Lysine content was obtained by cloning genes from
(a) E. coli and Corynebacterium (b) Corynebacterium alone (c) E. coli alone
(d) E. coli and Yeast
4. The metabolic precursor of polyhydroxy butyrate is
(a) propionyl CoA (b) acetyl CoA (c) butryl CoA (d) acetoacetyl CoA
5. cAMP controls the action of glycogen phosphorylase through
(a) activation process (b) inactivation process (c) both (a) & (b)
(d) none of the above
II. State True or False, if false give reason
(5 x 1 = 5)
6. ITCHY is a method of protein engineering
7. Secondary metabolites cannot be obtained by protein engineering
8. Structural genomics can be used for finding out the three dimensional structure of a protein
9. Pyruvate kinase is an irreversible enzyme in glycolysis.
10. The opposition to flux alterations at nodes is called nodal rigidity.
III. Complete the following:
(5 x 1=5)
11. PDB is a database for ______________________________________
12. Carotenoids are _________________________metabolites
13. NLS stands for
___________________
14. _________ is an important regulatory enzyme in gluconeogenesis.
15. A glycoprotein present in Chinese hamster cells ______________
IV. Define the following in one or two sentences within 50 words only (5 x 1=5)
16. What is the function of Phosphatase
17. What are the various types of genomics
18. What is the use of site directed mutagenesis in protein engineering?
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19. What is metabolic engineering?
20. Name the important regulatory enzymes of glycolysis.
PART B
V. Answer any five questions within 350 words only
(5 x 8= 40)
21 What are all the databases used for Comparative genomics
22. Give an account on Gene shuffling
23. Write a note on OMIM (Online Mendelian Inheritance in Man)
24. What are the various types of DNA binding proteins. Give one example for each type
25. Discuss covalent modification with one example.
26. What are nodes? Mention different types of nodes.
27. Describe gluconeogenesis pathway with regulation.
28. Explain competitive and noncompetitive inhibition.
PART C
VI. Answer the following in detail, not more than 1500 words each (2x 20= 40)
29. (a) Describe the original method of gene shuffling
OR
(b) Describe functional genomics
30 (a) Explain any two biosynthetic pathway with regulation
OR
(b) Describe the applications of cloning and expression of foreign gene in
metabolic engineering
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