LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO-MEDICAL INSTRUMENTATION SCIENCE
FIRST SEMESTER – NOVEMBER 2012
BI 1814 - TOOLS AND TECHNIQUES IN CELL BIOLOGY
Date : 01/11/2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
Section - A
Answer ALL of the following:
1. Define fluorescence.
2. Enumerate the components of a phase contrast microscope.
3. What is chromatin?
4. What are carcinogens? Give examples.
5. Define Svedberg constant.
6. Differentiate C banding and T banding.
7. Enlist the applications of stem cell technology.
8. Comment on flow cytometry.
9. Describe end labeling of probes.
10. Explain biothesiometry.
Section- B
Answer any FOUR of the following:
2 x 10 = 20
4 x10 = 40
11. What is micrometry?
12. Write a note on the checkpoints of a cell cycle.
13. Discuss the instrumentation and applications of an analytical centrifuge.
14. Explain FACS.
15. Describe Southern Blotting.
16. Give the structure and functions of plasma membrane.
Section-C
Answer any TWO of the following:
2 x 20 = 40
17. Comment on (a) Instrumentation and applications of a fluorescent microscope
(b) Any two methods adopted for histochemical staining.
18. Describe the
(a) Organization of chromatin.
(b) Properties of cancer cells.
19. Explain on the formation of density gradients and the types of density gradient centrifugation.
20. Explain PCR, its variants and its applications.
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