Curriculum Vitae Matthew R McIlvin Research Associate III Dept. of Marine Chemistry and Geochemistry Woods Hole Oceanographic Institution Woods Hole, MA 02543 Email: mmcilvin@whoi.edu Phone: (508) 289-2884 Education: Ph.D. in Analytical Chemistry, 2008. University of Massachusetts, Lowell, MA. B.S. in Chemistry, B.S. in Mathematics, 1998. University of Massachusetts, Dartmouth, MA. Work Experience: Research Associate, Saito lab: 2010-present, Woods Hole Oceanographic Inst. Performed sample analysis using Q-exactive, LTQ, TSQ, and ICP mass spectrometers. Analysis and data processing for proteomics and metals on large sample sets. Adapted multi-dimensional liquid chromatography methods for protein analysis on cultured bacteria and field samples. Maintained and repaired mass spectrometry, liquid chromatography, electrochemical and other laboratory equipment. Research Associate, Casciotti lab: 2004-2010, Woods Hole Oceanographic Inst. Managed biogeochemical laboratory including all equipment maintenance, data and sample management, and supervision of post docs and graduate students. Operated stable isotope facility that earned approximately $35,000 per year running nitrate isotope samples for outside clients and ran over 10,000 samples for internal laboratory experiments. Methods developed: a more cost-effective, faster method of oxygen isotopic analysis of water; a simpler method for hydroxylamine concentration in seawater; and, the first method for nutrient and isotopic analysis of individual benthic foraminifera. Equipment designed and built: first automated system for concentration and isotopic analysis of dissolved N2O in water samples; automated N2O headspace purge and trap system interface for IRMS; and, incubation chambers for labeled isotope experiments with marine sponges. Organized and prepared for seven major oceanographic research expeditions to South Pacific, North Pacific, North Atlantic, South Atlantic, and Indian Oceans. Certified for scientific diving at WHOI, 2007. Over 3 months shipboard sampling and groundwater sampling. Research Assistant, 1998-2004, Center for Marine Science and Tech., New Bedford, MA 6 years experience in a marine chemistry laboratory while working towards Ph.D. Developed novel methods for nitrogen and oxygen isotopic analysis of nitrate and nitrite in seawater and freshwater. Performed regular and major maintenance on two isotope ratio mass spectrometers, GC, and HPLC. Installed new EA-IRMS, purge and trap IRMS, and quadrupole MS. Enhanced GC-IRMS interface for nitrogen and carbon isotopic analysis of amino acids. Designed and built autosampler and nitrous oxide trapping system for IRMS. Over 4 months shipboard sampling and analysis in Atlantic and Southern oceans. Marine Mechanic, 1990-2005, Ron’s Marine Service, Chatham, MA Worked part to full time in a family business. Repaired and rebuilt boat engines and electrical systems. Teaching Experience: Umass Dartmouth Chemistry Dept., North Dartmouth, MA Instructor, Chemistry Lab and Organic Chemistry recitation (2001) Laboratory Teaching Assistant (1997-2002) o physical chemistry (4 semesters), o analytical chemistry (2 semesters), o organic chemistry o general chemistry (3 semesters) Publications: Mackey KRM, Paytan A, Caldeira K, Grossman A, Moran D, McIlvin MR, Saito MA, Effect of temperature on photosynthesis and growth in marine Synechococcus, PLANT PHYSIOLOGY, August 2013 pp.113.221937. Aguirre JD, Clark HM, McIlvin MR, Vazquez C, Palmere SL, Grab DJ, Seshu J, Hart PJ, Saito MA, Culotta VC, A Manganese-rich Environment Supports Superoxide Dismutase Activity in a Lyme Disease Pathogen, Borrelia burgdorferi, JOURNAL OF BIOLOGICAL CHEMISTRY, Volume 288, Issue 12, Pages 8468-8478, March 2013. Casciotti KL, Buchwald C, McIlvin MR, Implications of nitrate and nitrite isotopic measurements for the mechanisms of nitrogen cycling in the Peru oxygen deficient zone, DEEP SEA RESEARCH Part I: Oceanographic Research Papers, Volume 80, 2013, Pages 78–93. Bertrand EM, Moran DM, McIlvin MR, Hoffman JM, Allen AE, Saito MA, Methionine synthase interreplacement in diatom cultures and communities: Implications for the persistence of B12 use by eukaryotic phytoplankton, LIMNOLOGY AND OCEANOGRAPHY, 58(4), 2013, 1431-1450. Buchwald C, Santoro AE, McIlvin MR, Casciotto CL, Oxygen isotopic composition of nitrate and nitrite produced by nitrifying cocultures and natural marine assemblages, LIMNOLOGY AND OCEANOGRAPY, 57(5), 2012, 1361-1375. Bernhard JM, Casciotti KL, McIlvin MR, Beaudoin DJ, Visscher PT, Edgcomb VP, Potential importance of physiologically diverse benthic foraminifera in sedimentary nitrate storage and respiration, JOURNAL OF GEOPHYSICAL RESEARCHBIOGEOSCIENCES, 117, 2012, Article Number: G03002 DOI: 10.1029/2012JG001949. Bernhard JM, Edgcomb VP, Casciotti KL, McIlvin MR, Beaudoin DJ, Denitrification likely catalyzed by endobionts in an allogromiid foraminifer, ISME JOURNAL, 6(5), 2012, 951-960. Santoro AE, Buchwald C, McIlvin MR, Casciotti KL, Isotopic Signature of N2O Produced by Marine Ammonia-oxidizing Archaea. SCIENCE, 333(6047), 2011, 1282-1285. McIlvin MR, Casciotti KL, Technical Updates to the Bacterial Method for Nitrate Isotopic Analyses, ANALYTICAL CHEMISTRY, 83(5), 2011, 1850-1856. Casciotti KL, McIlvin MR, Buchwald C, Oxygen isotopic exchange and fractionation during bacterial ammonia oxidation, LIMNOLOGY AND OCEANOGRAPHY, 55(2), 2010, 753-762. McIlvin MR, Casciotti KL, Fully automated system for stable isotopic analyses of dissolved nitrous oxide at natural abundance levels, LIMNOLOGY AND OCEANOGRAPHY-METHODS, 8, 2010, 54-66. Casciotti KL, McIlvin MR, and Buchwald C., Oxygen isotopic exchange and fractionation during bacterial ammonia oxidation, LIMNOLOGY AND OCEANOGRAPHY, 55(2), 2010, 753–762. Higgins MB, Robinson RS, Casciotti KL, McIlvin MR, et al., A Method for Determining the Nitrogen Isotopic Composition of Porphyrins, ANALYTICAL CHEMISTRY 81(1), 2009, 184-192. Casciotti KL, McIlvin MR, Isotopic analyses of nitrate and nitrite from reference mixtures and application to Eastern Tropical North Pacific waters, MARINE CHEMISTRY, 107(2), 2007, 184-201. Casciotti KL, Bohlke JK, McIlvin MR, et al., Oxygen isotopes in nitrite: Analysis, calibration, and equilibration, ANALYTICAL CHEMISTRY, 79(6), 2007, 24272436. McIlvin MR, Casciotti KL, Method for the analysis of delta O-18 in water, ALYTICAL CHEMISTRY, 78(7), 2006, 2377-2381. McIlvin MR, Altabet MA, Chemical conversion of nitrate and nitrite to nitrous oxide for nitrogen and oxygen isotopic analysis in freshwater and seawater, ANALYTICAL CHEMISTRY, 77(17), 2005, 5589-5595