MCB 5505/BSC 4933
VIROLOGY
SAMPLE TEST 1 - BACTERIOPHAGE
R. H. REEVES
THE FIRST THIRTY QUESTIONS ARE WORTH TWO POINTS EACH.
THERE IS
ONLY ONE CORRECT ANSWER FOR EACH QUESTION.
PLEASE CIRCLE THE
CORRECT LETTER OR PLACE THE CORRECT LETTER JUST TO THE LEFT OF THE
QUESTION NUMBER.
1.
HE CRYSTALLIZED A PURIFIED PLANT VIRUS AND HELPED FUEL THE
HEATED DEBATE OVER WHETHER LIVING SYSTEMS COULD BE COMPLETELY
DESCRIBED IN PHYSICAL AND CHEMICAL TERMS.
A.
PEYTON ROUS
B.
WENDELL STANLEY
C.
A. D. HERSHEY
D.
D. CARLETON GAJDUSEK
E.
JONAS SALK
2.
MOST
A.
B.
C.
D.
E.
3.
CIRCULAR DNA IS FOUND IN THE VIRIONS OF
A.
BACTERIOPHAGE LAMBDA
B.
T7
C
X-174
D.
THE LEVIVIRIDAE
E.
T2
4.
AN ICOSAHEDRAL VIRUS WITH A TRIANGULATION NUMBER OF SEVEN HAS
___ HEXON CAPSOMERS
A.
30
B.
40
C.
50
D.
60
E.
70
5.
THE GENOMIC DNA OF BACTERIOPHAGE LAMBDA
A.
IS DOUBLE STRANDED
B.
HAS STICKY ENDS
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
6.
BACTERIOPHAGE T7
A.
MAKES ITS OWN RNA POLYMERASE
B.
MAKES ITS OWN DNA POLYMERASE
ENVELOPED VIRUSES
CONTAIN BOTH RNA AND DNA
CONTAIN HOST MEMBRANE PROTEINS
ARE INSENSITIVE TO ORGANIC SOLVENTS
CONTAIN VIRAL GLYCOPROTEINS
ALL OF THE ABOVE
C.
D.
BOTH OF THE ABOVE
NEITHER OF THE ABOVE
KEY FOR QUESTIONS 7-16 THERE IS ONLY ONE CORRECT ANSWER BUT EACH
KEY WORD MAY BE USED MORE THAN ONCE.
A.
B.
C.
D.
E.
F.
G.
H.
T2
MS2
M13
Q
T7
LAMBDA
TOBACCO MOSAIC VIRUS
X-174
_____07
ITS DNA HAS cos AND att SITES
_____08.
A HELICAL VIRUS WITH A SINGLE-STRANDED DNA GENOME
_____09.
ITS GENES ARE EXPRESSED IN THE ORDER THEY APPEAR ON THE
GENOME
_____10.
ITS LYSIS PROTEIN GENE OVERLAPS BOTH ITS COAT AND
REPLICASE GENES
_____11.
MAKES AN "IMMUNITY SUBSTANCE"
_____12.
A PODOVIRUS
_____13.
ITS FOURTH GENE IS MADE BY A “READ THROUGH” OF A UGA
STOP CODON
_____14.
VIRION HAS A CONTRACTILE TAIL
_____15.
MEMBER OF THE SIPHOVIRIDAE
_____16.
MAKES ITS OWN DNA-DEPENDENT RNA POLYMERASE
22.
EXPLAIN HOW THE TRANSLATION OF THE COAT PROTEIN AND THE COAT
PROTEIN ITSELF REGULATE THE PRODUCTION OF THE RNA REPLICASE
IN PHAGE MS2.
23.
HOW DOES THE CII GENE PRODUCT REGULATE THE PRODUCTION OF THE
LAMBDA REPRESSOR AND THE INTEGRASE?
24.
BOTH LAMBDA AND X-174 REPLICATE THEIR DNA BY THE ROLLING
CIRCLE MODEL OF DNA REPLICATION; HOWEVER, THE END RESULT OF
THIS REPLICATION IS VERY DIFFERENT. ONE PHAGE HAS UNIQUE, DS
DNA IN THE VIRION AND THE OTHER HAS A VIRION GENOME WHICH IS
A SS CIRCLE. EXPLAIN. (PLEASE USE DIAGRAMS)
25.
WHAT IS THE FUNCTION OF THE TERMINAL REPEATS ON THE ENDS OF
T7 DNA?