C383 Study Guide for Exam 2
Spring 2016
Basic Information
 Exam 2: Thursday, March 10, 7:15-9:15PM in Chemistry 122
 Arrive early for assigned seats
 Bring your student ID. Failure to do so will result in getting your exam back later.
 You may use a NON-PROGRAMMABLE calculator.
 All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
Exam Content: The exam will cover chapters 7-14. All material covered in class, homework, and
discussion sections could be on the exam. Some major topics include:
Michaelis-Menton kinetics, saturation curves, Km, kcat, turnover number, kcat/Km, catalytic
efficiency, Lineweaver-Burk plots, allosteric enzyme, cooperativity, tense/relaxed, sigmoidal
curves, feedback inhibition, temperature and pH effect on enzyme rates, irreversible inhibition,
group-specific reagents, affinity reagents, transition state inhibitors, competitive inhibitors,
uncompetitve inhibitors, noncompetitive inhibitors, mixed inhibitors, penicillin, chymotrypsin
mechanism, catalytic triad, oxyanion hole, hemoglobin structure, myoglobin, oxygen binding
curves, 2,3-BPG, effect of proton and carbon dioxide on hemoglobin oxygen binding, sickle cell
anemia, monosaccharide structure, stereochemistry, epimers, anomers, cyclic monosaccharide
structures, disaccharide and polysaccharide, glycoprotein functions and pathologies, fatty acid
and triacylglyceride structure, phospholipid, glycolipid, cholesterol, lipid bilayer, membrane
fluidity, integral protein structure, anchored proteins, transverse diffusion, sodium/potassium
pump, passive transport, active transport, secondary active transport, structural basis of ion
channel selectivity, ligand/receptor binding, receptor tyrosine kinase, transducer, G-protein,
second messenger, cAMP, kinase cascade, deactivation of signal transduction, Ras, constitutive
activity, digestion, zymogens, hydrolysis of macromolecules, digestive roles of organs, protease,
lipase, amylase, role of transporters in intestinal cells, chylomicrons
Exam Format: The exam is out of 100 points. The first 60 points will be multiple choice, short
answer, fill in the blank type questions. There will be four 10-point problems involving
calculations, structural drawings, or short essay. There will be conceptual and quantitative
problems. Thermodynamic equations and constants will be given.