EXPRESSION OF NM23 ANTIMETASTATIC GENE
PRODUCT IN PROSTATE CARCINOMA IN SAUDI
PATIENTS AND ITS RELATION TO CHROMOSOMAL
INSTABILITY
BACKGROUND: The nm23 gene was initially cloned as a
metastasis suppressor gene, but the clinical relevance of nm23 as a
prognostic indicator for human cancers remains controversial.
Chromosomal instability (CIN) is a high frequency of
chromosomal loss and gain that has been demonstrated in
dysplastic and neoplastic lesions. Recently CIN has been shown in
prostate carcinoma (Pca) and its precursor. The objectives of this
project are: firstly to study the nm23 expression pattern in Pca and
to study the relation between nm23 expression and CIN.
METHODS: Immunohistochemistry with a polyclonal antibody
was employed to study the expression of the nm23 protein in
formalin-fixed, paraffin-embedded tissue specimens obtained from
patients with Pca (n: 28), collected from the department of
Pathology at King Abdulaziz Hospital (Jeddah, Saudi Arabia). CIN
was studied by using dual-color interphase fluorescence in situ
hybridization (IFISH) performed on archived Pca.
RESULTS: Expression of nm23 was noted in 24 out of 28 Pca
(85%). Five of the patients have evidence of metastasis and four of
them showed strong nm23 expression. CIN was detected in 8 out of
18 cases with interpretable IFISH signals. Nm23 expression was
seen in 7/8 of CIN positive and in 8/10 of the CIN negative cases.
Nm23 positivity was seen in 6/8 cases of Gleason score 6, 12/14
cases of score 7, 4/4 cases of score 8 and 2/2 cases of score 9.
Normal and hyperplastic tissue show either negative or weak
cytoplasmic staining.
CONCLUSIONS: The results suggest that expression of nm23 in
Pca is not correlated to tumor metastasis in contrast to carcinoma in
other organs. While our data suggest that nm23 may help in the
distinction of normal from malignant prostatic tissue, these results
do not point to nm23 as a reliable prognostic marker in Pca. Nm23
‫عنوان الوثيقة‬
Document (
)Title
‫المستخلص‬
)Abstract(
is not correlated to the CIN in Pca.
)ISSN( ‫ردمد‬
‫اسم الدورية‬
)Journal Name(
)Volume( ‫المجلد‬
37
3&4
2006
118 : ‫إلى‬
107
: ‫من‬
‫العدد‬
)Issue Number(
‫سنة النشر‬
Publishing (
)Year
‫الصفحات‬
)Pages(
Step 1
Step 2
: )‫(ع‬
Jaudah
: )E(
: )‫(ع‬
Al-Maghrabi,
: )E(
Department of Pathology, College of Medicine, King Abdul Aziz University
Hospital; Jeddah, Saudi Arabia
jalmaghrabi@hotmail.com
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First name of the
researcher
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researcher
‫العنوان‬
)Address)
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