1406 Guide. Ch 6-11.doc

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Study Guide for Biology 1406

Second exam

Dr. Chukwu

Chapter Six

A Tour of the Cell

Define a cell

Differentiate between unicellular and multi-cellular, animal and plant cells

How do we study cell function

What do you mean by fracturnation?

Name the types and parts of the microscope

The three main parts of the cell

Organelles and their functions

The cell cycle, why do cells divide?

The characteristic the plasma membrane

The lipid by-layer and fluid mosaic

What are the parts of the control center of the cell

What are the functions of the plant cell wall

Name the 3 main types of intercellular links Animal cell, what are their functions

What are “The cell theory”

What are the differences between the Eukaryotes & Prokaryotes

Chapter Seven

Membrane Structure and Function

Membrane Transport

What is the plasma membrane made up of

What do you mean by amphipathic

What are the functions of the cholesterol molecules and protein

What is selective permeability

 Explain “Diffusion depends on concentration gradients”

What is dynamic equilibrium?

Hydrophobic vs Hydrophobic

What do you mean by passive

What are Osmosis, facilitated transport, dynamic equilibrium, concentration gradient, active transport

Membrane junctions, three types

What are Hypertonic, Hypotonic and Isotonic solutions

Plasma membrane, Phospholipids, amphipathic

What strengthens the fluid mosaic and makes it more stable

What are Diploid and Haploid Number of chromosomes

Where does the gene reside, Sex and body chromosomes

Define mitosis and meiosis

What do yo mean by crossing over

Mother cells vs daughter cells

The Human somatic or Body cells and sex cells, the phases of cell division

What is binary fission?

Tell what is happening at each phase of cell division PMAT, Interphase

What is crossing over, when does this occur

What is Cytokinesis

Differentiate between mitosis and Meiosis

Chapter 8 Metabolism

Metabolism refers to all to the chemical reactions within a cell

These include catabolic and anabolic reactions

Catabolism : this is the chemical reaction that breaks down large substances into smaller ones with the release of energy

Anabolism : this is the systemic chemical reactions in which the products are larger than the reactants, ex aa to protein

Energy coupling: this is the use or transfer of energy generated during catabolism (exergonic reaction) to drive anabolism (endergonic reaction)

Energy

Energy is the ability to do work

Energy exists in many forms

Kinetic energy: this is the energy of motion

Potential energy: this is the energy of position

Chemical energy: this the energy stored in molecules

There are also mechanical, heat and light energy, radient

Energy can be transformed from one form to another

Bioenergetics : this is the study of how organisms manage their energy resources

Thermodynamics : this is the study of the energy transformation that occur in a collection of matter

First law of energy : energy cannot be created or destroyed, but can be transformed or transferred

Second law of energy : every energy transfer or transformation increases the entropy

(disorderliness) of the universe

Third law of energy

: the quantity of energy in the universe is constant, but it’s quality is not

Spontaneous VS non-spontaneous process

A spontaneous process can take place with out any outside help, ex. The flow of water down the hill

A non-spontaneous process cannot occur on it’s own.

Energy is expended for this to take place, ex. Machine pumping water into a water tank tower

What is Free energy :

The proportion of the system energy that can perform work when temperature is uniform throughout

What is Exergonic reaction : this is a spontaneous chemical reaction in which there is a net release of free energy

What is Endergonic reaction : this is the type of reaction in which free energy is absorbed from the surrounding ( a non-spontaneous chemical reaction)

ATP (Adenosine Triphosphate ): this is the energy source for the cells

It is composed of a nitrogen base adenine, the sugar ribose and three phosphate group

ATP is used in the body cells for anabolic reactions, active transport, nervous conducting and muscle contraction.

ADP (Adenosine Diphosphate): it is similar in structure to ATP except for the phosphate group

ATP has 3p, whereas ADP has 2 phosphate group

ADP is produced when ATP is hydrolyzed

Enzymes

An enzyme is a protein catalyst that speeds up chemical reaction

They regulate the speed at which a chemical reaction occur without affecting the end product of the reaction and without being used up in the reaction

Energy of activation : this is the energy that must be provided to cause molecules to react with one another

Enzymes are substrate specific

Wht is a substrate? is a reactant in a reaction controlled by an enzyme

Ex. Reactant enzyme

Substrate Product

Sucrase

Sucrose + Water Glucose +Fructose

Factors affecting enzyme activity

Temperature : - rate of reaction decreases with rise in temperature

Enzyme is denatured beyond optimal temperature

Ph : the optimal values ranges from 6 – 8 with a few exception

For pepsin the optimal ph is 2

Cofactor : enzyme may be bound tightly to active site as permanent residents

Inhibitors: they are mimics

Competitive inhibitors reduce the productivity of enzymes

Enzymes may block substrates from entering the active site

Chapter 9

What are cellular respiration, mitochondria

The overall process is:

Organic compounds + O2 CO2 + H2O + Energy

Glucose.

C6H12O6 + 6O2 6CO2 + 6H2O + Energy (ATP + heat)

686 kcal per mole of glucose

What is Phosphorylation

What is Oxidation-reduction reactions, or redox reactions

The three metabolic stages of respiration are ?

In which part of the cell do Glycolysis and krebs cycle occur

How many ATP produced by one mole of glucose?

How many % of ATP is used for work and heat?

Chapter 10 Photosynthesis

Photosynthesis is the conversion of light energy to chemical energy which is stored in the form of glucose or other organic compounds

Photosynthesis occur in plants, algae and certain prokaryotes

6CO2 + 12H2O + light energy C6H12O6 + 6O2 + 6H2O

Where does photosynthesis occur

Light vs dark cycle

An autotroph is an organism that obtains organic food molecules without eating other organisms

An autotroph is a primary producer or biosphere producer

Autotrophs use energy from the sun or from oxidation of inorganic substances to make organic molecules

Heterotroph s

They are organisms that obtain food molecules by eating other organisms or their by products

They are the biosphere consumers

Decomposers

These are saprotrophic fungus and bacteria that absorb nutrients from non-living organic materials and convert them into inorganic forms (fungi and bacteria)

Plants usually store their food in the form of starch

Photoautotrophs (light – self – feeding)

Almost all autotrophs depend on photosynthesis and the byproduct of photosynthesis for food and oxygen

Chloroplast : the green coloring matter in plants green leaves, stem and fruits

Chloroplast is an organelle found in plants and photosynthetic protists that absorb sunlight and uses it to drive the synthesis of organic compounds from CO2 and H2O

Chlorophyll: this is the pigment in the chloroplast of plants

Chlorophyll is directly involved in light reaction

It converts solar energy into chemical energy

Light reaction is a Redox reaction – the reverse of ATP production

C6H12O6 + 6O2 = 6CO2 + H2O + Energy

Two stages of photosynthesis

Light reaction converts solar energy to chemical energy

Electron and hydrogen are transferred from water to NADP

Oxygen is released in this process

ATP and NADP are produced in the light reaction

A phosphate group is added to ADP (phosphorylation )

No sugar is produced

Carbon fixation

This is the incorporation of carbon into organic compound

This is the beginning of the Calvin cycle

The fixed carbon is reduced by addition of electrons (energy from NDP)

Carbon dioxide is converted to carbohydrate using the ATP generated from the light cycle

Therefore, calvin cycle produces CHO in plants

No light is required (dark reaction

Light cycle: in the thylakoides of the chloroplast

Calvin cycle: in the stoma

Chapter 11 Cell communication

Cells must communicate in order to coordinate their activities in a way that enables the organisms to develop from a fertilizes egg and then survive and reproduce in turn

What is a target cell?

Cell signaling: a method of regulation in all living things

Signaling originating from another cell or from some changed in the organisms physical environment can be received by cells in various forms

Receptors

Cells respond to electromagnetic signals – light

Cells respond to mechanical signals – touch

Cells most often communicate with each other using chemical signals

A signal from cell is converted into specific cellular response in a step called signal transduction pathway

Cells communicate by releasing chemical messengers targeted for cells that may not be immediately adjacent

Intracellular communication

Neural communication

Neurotransmitter: transmits impulse along the nervous system (chemical signal) ex ACH

The impulse diffuse into the nerve cell and passed from one nerve fiber to another across the narrow synaptic cleft (gap)

What is a neurotransmitter, give example

Endocrine communication

Hormone or endocrine signaling

A specialized cell releases the hormone into the blood system which transport it to the target cells; ex. Insulin and the reproductive hormones (gonadotropic - LH, FSH (ICSH)

Paracrine communication :

The product of the cells diffuse into the extracellular fluid (ECF) to affect neighboring cells that may be some distance away

Autocrine communication :

Cells secret chemical messengers that bind to receptors on the same cell

In paracrine signaling, numerous cells can simultaneously receive a response to the molecules of growth factor produced by a single cell on their vicinity (growth factor)

Animal cells may communicate via direct contact between molecules on their surfaces

This is important in embryo development and operation of the immune system

Three stages of cell signaling

Receptor :

The target cell that detects the signal from outside the cell

A chemical signal is detected when it binds to a cellular protein

Molecule binding initiates the process of Transduction

Transduction : converts received signal to a form that can bring about a specific response

This may occur in a single step

Often requires a sequence of changes in a series of different molecules

Response: transduced signal triggers a specific cellular response

Ligand : a molecule that binds specifically to a receptor site of another molecule

***Ethylene*** this is a plant hormone (C2H4) that promote fruit ripening, a hydrocarbon with only six atoms

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