Suppl. Fig. 3
Impact of ECS on H3K27 trimethylation. (a) The amount of the histone mark H3K27me3 was
analyzed at different time points after ECS or sham treatment (sh) by Westernblot and
normalized to histone 3 (H3) protein levels. β-actin was used as independent loading control.
Densitometric quantification (bar graph) did not show significant changes in global H3K27me3
methylation relative to the corresponding sham controls (white bars) using one-way ANOVA
and Tukey test for multiple comparison. n=3. Error bars represent mean +/- SEM. (b)
Immunofluorescence staining of H3K27me3 (visualized in red, left panel) did not reveal obvious
local changes in H3K27 trimethylation in the hippocampus at 0 h (upper row) and 24 h (middle
row) after ECS. DAPI was used to counterstain nuclei (blue, middle panel). Merged pictures are
shown in the right panel. Primary antibody was omitted to control for background staining
(control, bottom row). Scale bar: 500 µm.
Suppl. Fig. 3: