What is the major issue of this paper?
A) How C. elegans embryos divide?
B) How germline specification is achieved?
C) What is PIE-1 protein?
D) Where is pie-1 mRNA is localized?
E) How the EMS blastomere arise?
How did the authors clone the pie-1 gene?
A) Through Antibody staining
B) Tc1 transposon insertion or tagging
C) C. elegans lineage analysis
D) They cloned the gene by pure luck
E) They cloned the gene by a candidate screen
What is the most important concept that you
learn from reading this paper?
A.
The localization patterns of PIE-1
B.
How pluripotency of a stem cell is maintained by a general repressor
C.
What kind of protein is PIE-1
D.
C. elegans is a good animal model for studying embryo development
E.
PIE-1 associates with centrosomes during cell division
How did the authors reach the conclusion that PIE-1
might be a transcriptional repressor?
A. from antibody staining
B. from cell lineage analysis
C. from protein sequence
D. it is just a wild guess
E. from its interaction with other somatic-determining genes
What are the important questions that the paper did not
address and need to be analyzed in subsequent study?
A. Whether PIE-1 is a transcription repressor
B.
How is PIE-1 selectively degraded in somatic daughters
C.
How does PIE-1 associate with centrosomes
D.
What are the repression targets of PIE-1
E.
All of the above