Anatomy & Physiology 34A Lab 3 - The Microscope I. Today’s lab objectives (what you should learn from this lab): A. Parts and care of light microscopes B. Relative magnifications of compound microscopes C. How to use a light microscope II. Activity 1 – Identifying microscope parts A. Compound microscope: used for viewing smaller 2-D, transparent specimens. Identify the microscope parts listed below (#1-11). See diagram on next page. B. Describe or define the microscope terms listed below 1. Eyepieces with ocular lenses 2. Objective lenses (3) on rotating nosepiece 3. Mechanical stage 4. Mechanical stage clips 5. Mechanical stage adjustment knobs 6. Substage light 7. Condenser lens 8. Condenser knob 9. Iris Diaphragm 10. Fine adjustment knob 11. Coarse adjustment knob (used only with which objective lens?) 12. Total magnification 13. Depth of field 14. Field of view 15. Resolution 16. Parfocal III. Activity 2: Viewing objects through the microscope - Do numbers 1-8 with letter “e” slide; omit #9 and measurements IV. Activity 3: OMIT V. Activity 4: Perceiving depth - Do this with threads slide VI. Activity 5: Preparing and observing a wet mount - Do this after I demonstrate it (cheek cell wet mount)