Anatomy & Physiology 34A
Lab 3 - The Microscope
I. Today’s lab objectives (what you should learn from this lab):
A. Parts and care of light microscopes
B. Relative magnifications of compound microscopes
C. How to use a light microscope
II. Activity 1 – Identifying microscope parts
A. Compound microscope: used for viewing smaller 2-D, transparent specimens. Identify
the microscope parts listed below (#1-11). See diagram on next page.
B. Describe or define the microscope terms listed below
1. Eyepieces with ocular lenses
2. Objective lenses (3) on rotating nosepiece
3. Mechanical stage
4. Mechanical stage clips
5. Mechanical stage adjustment knobs
6. Substage light
7. Condenser lens
8. Condenser knob
9. Iris Diaphragm
10. Fine adjustment knob
11. Coarse adjustment knob (used only with which objective lens?)
12. Total magnification
13. Depth of field
14. Field of view
15. Resolution
16. Parfocal
III. Activity 2: Viewing objects through the microscope - Do numbers 1-8 with letter “e”
slide; omit #9 and measurements
IV. Activity 3: OMIT
V. Activity 4: Perceiving depth - Do this with threads slide
VI. Activity 5: Preparing and observing a wet mount - Do this after I demonstrate it (cheek
cell wet mount)