Supplemental Tables
Supplemental Table S1. The SNP rs2854744 is in Hardy-Weinberg equilibrium
(HWE) in all study groups.
Study groups
Caucasians combined
Hungary
Germany combined
East Germany
West Germany combined
Number of subjects with complete
basis data (age, gender, smoking)
HWE ² P value
Status
N
Cases
1240 1192
0.9755
Controls 1318 1310
0.8999
Cases
222
0.6460
Controls 77
74
0.5589
Cases
970
0.9800
243
997
Controls 1241 1236
0.9777
Cases
212
212
0.9314
Controls 200
200
0.8384
Cases
758
0.9997
785
Controls 1041 1036
1.0000
Cases
387
381
0.9997
Controls 167
167
0.5656
Cases
398
377
0.9977
Controls 874
869
0.9033
DO - hospital
Cases
87
86
0.6522
DO - occupational
Cases
311
291
0.8330
DO - controls
Controls 874
869
0.9033
Cases
98
0.1711
Controls 219
56
0.4451
Cases
112
112
0.0967
Controls 188
188
0.9177
W. Germany - ongoing
W. Germany - industrial
Pakistan
Venezuela
All combined
Cases
98
1450 1402
0.6775
Controls 1725 1554
0.9978
1
Supplemental Table S2. Unadjusted ORs and 95% CIs for all relevant genetic
models indicate minor effects in Hungarians, Pakistanis and Venezuelans but no
reliable effect of rs2854744 A/A on bladder cancer.
A. Results of the additive model with [A] as risk factor.
Genotype frequency
Additive model
Study groups
Status
N
C/C
A/C
A/A
P value
Caucasians combined
Cases
Controls
1234
1318
0.31
0.31
0.50
0.50
0.20
0.19
0.7987
Hungary
Cases
237
0.28
0.53
0.20
0.3062
Controls
77
0.31
0.55
0.14
1.22
0.83 - 1.79
Cases
997
0.32
0.49
0.19
0.8372
Controls
1241
0.31
0.50
0.19
0.99
0.88 - 1.11
Cases
212
0.37
0.47
0.17
0.6832
Controls
200
0.34
0.51
0.16
0.94
0.71 - 1.25
Cases
785
0.30
0.50
0.20
0.8951
Controls
1041
0.30
0.49
0.20
1.01
0.88 - 1.15
Cases
387
0.28
0.50
0.22
0.9524
Controls
167
0.26
0.54
0.20
1.01
0.78 - 1.31
Cases
398
0.32
0.49
0.19
0.5627
0.95
0.80 - 1.13
Germany combined
East Germany
West Germany combined:
W. Germany - ongoing
W. Germany - industrial
OR,
95% CI
1.02
0.91 - 1.14
Controls
874
0.31
0.49
0.20
hospitala
Cases
87
0.31
0.45
0.24
0.5992
1.09
0.80 - 1.48
DO - occupationala
Cases
311
0.32
0.50
0.17
0.3501
0.92
0.76 - 1.10
DO - controls
Controls
874
0.31
0.49
0.20
Cases
98
0.37
0.40
0.23
0.0560
Controls
219
0.44
0.42
0.14
1.37
0.99 - 1.90
Cases
Controls
Cases
112
188
1450
0.40
0.37
0.32
0.38
0.49
0.48
0.21
0.14
0.20
0.6724
1.07
0.77 - 1.49
0.3734
1.05
0.95 - 1.16
Controls
1725
0.33
0.49
0.18
DO -
Non Caucasian study groups
Pakistan
Venezuela
All combined
aControls
are the Dortmund control group
OR = odds ratio; 95% CI = 95% confidence intervals
P values of the exact Cochran-Armitage trend test (2-sided), ORs and 95% CIs for the additive model
are given. The combined Caucasian study groups have been adjusted for different regions (Hungary,
East Germany, West Germany). All study groups combined have been adjusted for different
ethnicities.
2
B. Results of the recessive model for [A] and the multiplicative model with [A] as risk
factor.
Genotype
Recessive model
Multiplicative model
OR
95% CI
1.05
0.86 1.28
1.48
0.70 3.34
1.00
0.81 1.24
1.04
0.59 1.82
1.01
0.80 1.28
1.14
0.72 1.86
0.91
0.66 1.24
1.26
0.71 2.16
0.81
0.57 1.15
P value
1.86
0.97 3.53
1.63
0.84 3.12
1.12
0.94 1.34
0.0508
Study groups
Status
N
A/A
[A]
P value
Caucasians
combined
Cases
Controls
1234
1318
0.20
0.19
0.44
0.44
0.6429
Hungary
Cases
237
0.20
0.46
0.3158
Controls
77
0.14
0.42
Cases
997
0.19
0.44
Controls
1241
0.19
0.44
Cases
212
0.17
0.40
Controls
200
0.16
0.41
West Germany
combined
Cases
785
0.20
0.45
Controls
1041
0.20
0.45
W. Germany ongoing
Cases
387
0.22
0.47
Controls
167
0.20
0.47
W. Germany industrial
Cases
398
0.19
0.43
Controls
874
0.20
0.44
DO - hospitala
Cases
87
0.24
0.47
0.4032
DO - occupationala
Cases
311
0.17
0.42
0.2431
DO - controls
Controls
874
0.20
0.44
Cases
98
0.23
0.43
Controls
219
0.14
0.35
Venezuela
Cases
Controls
112
188
0.21
0.14
0.41
0.39
0.1521
All combined
Cases
1450
0.20
0.44
0.2062
Controls
1725
0.18
0.42
Germany combined
East Germany
1.0000
0.8947
0.9530
0.5747
0.5434
0.7996
0.3531
0.8558
0.7227
0.9197
1.0000
0.5757
0.6316
0.3715
OR
95% CI
1.01
0.91 1.14
1.20
0.82 1.77
0.99
0.88 1.11
0.94
0.71 1.26
1.01
0.88 1.15
1.01
0.77 1.31
0.95
0.80 1.13
1.09
0.79 1.51
0.92
0.76 1.11
Non Caucasian study groups
Pakistan
aControls
0.0520
0.6670
0.3717
1.43
0.9951 2.0403
1.08
0.76 1.53
1.05
0.95 1.16
are the Dortmund control group
OR = odds ratio; 95% CI = 95% confidence intervals
P values of the exact chi-square test, ORs and 95% CIs for the recessive (A/A vs. A/C, C/C) and of
the multiplicative ([A] vs. [C]) model are given. P values for combined Caucasian groups adjusted for
region and P values for all study groups combined adjusted for ethnicity were calculated using the
method of Mantel-Haenszel. Breslow-Day tests indicate the homogeneity of the ORs (recessive
model: Caucasians combined P=0.6090, all combined P=0.0829; multiplicative model: Caucasians
combined P=0.5800, all combined P=0.1717).
3
Supplemental Table S3. No effect of the rare [A] allele of the IGFBP3 SNP
rs2854744 adjusted for age, gender and smoking habits.
A. Adjusted P values, ORs and 95% CIs for the additive model.
Study groups
Status
Caucasians combined
Cases
1192 0.31
Controls 1310 0.31
0.50
0.50
0.20
0.19
0.8637
1.01
0.90 - 1.14
Hungary
Cases
0.28
0.53
0.20
0.1200
Controls 74
0.31
0.55
0.14
1.41
0.92 - 2.16
Cases
0.32
0.49
0.19
0.8060
Controls 1236 0.31
0.50
0.19
0.98
0.87 - 1.12
Cases
0.7503
0.95
0.71 - 1.28
0.8527
0.99
0.86 - 1.14
0.7212
0.95
0.72 - 1.25
0.2890
0.90
0.75 - 1.09
Germany combined
East Germany
222
970
Genotype frequency P value OR,
95% CI
C/C
A/C
A/A
212
0.37
0.47
0.17
Controls 200
0.34
0.51
0.16
0.30
0.50
0.20
Controls 1036 0.30
0.49
0.20
Cases
West Germany combined Cases
W. Germany - ongoing
N
758
381
0.28
0.50
0.22
Controls 167
0.26
0.54
0.20
Cases
377
0.32
0.49
0.19
Controls 869
0.31
0.49
0.20
DO - hospitala
Cases
86
0.31
0.45
0.24
0.4024
1.15
0.83 - 1.61
DO - occupationala
Cases
291
0.32
0.50
0.17
0.1074
0.84
0.68 - 1.04
DO - controls
Controls 869
0.31
0.49
0.20
98
0.37
0.40
0.23
0.7960
Controls 56
0.44
0.42
0.14
0.94
0.58 - 1.52
Cases
Controls
Cases
Controls
0.40
0.37
0.32
0.33
0.38
0.49
0.48
0.49
0.21
0.14
0.20
0.18
0.7262
1.09
0.67 - 1.77
0.6475
1.03
0.92 - 1.15
W. Germany - industrial
Non Caucasian study groups
Pakistan
Venezuela
All combined
Cases
112
188
1402
1554
aControls
are the Dortmund control group (DO controls)
OR = odds ratio; 95% CI = 95% confidence intervals
P values of the Wald test, ORs and 95% CIs for the additive model adjusted for age,
gender and smoking habits are given. N denotes the number of observations used for
the multivariable logistic regression model. The combined Caucasian study groups
have been adjusted for different regions (Hungary, East Germany, West Germany).
All study groups combined have been adjusted for different ethnicities.
4
B. Adjusted P values, ORs and 95% CIs for the recessive mode of inheritance for the
risk allele [A] and the multiplicative model for the risk allele [A].
Multiplicative model
A/A
OR
95% CI
1.04
0.84 - 1.29
P value
1.86
0.84 - 4.12
0.1377
1.36
0.91 - 2.04
0.99
0.79 - 1.24
0.8060
0.98
0.87 - 1.12
1.15
0.66 - 2.01
0.7522
0.95
0.71 - 1.28
0.96
0.75 - 1.24
0.8528
0.99
0.86 - 1.14
1.04
0.65 - 1.66
0.7264
0.95
0.73 - 1.25
0.83
0.59 - 1.17
0.2870
0.90
0.74 - 1.09
1.38
0.79 - 2.41
0.71
0.48 - 1.05
0.3980
0.87
0.62 - 1.21
1.19
0.96 - 1.47
1.04
0.42 - 2.56
0.7871
0.93
0.56 - 1.55
1.69
0.70 - 4.06
0.7230
1.09
0.67 - 1.78
1.09
0.90 - 1.32
0.6474
1.03
0.92 - 1.15
Study groups
Status
Caucasians combined
Cases
1192 0.20 0.44 0.7267
Controls 1310 0.19 0.44
Hungary
Cases
Germany combined
N
Genotype Recessive model
222
[A]
P value
0.20 0.46 0.1286
Controls 74
0.14 0.42
Cases
0.19 0.44 0.9307
970
Controls 1236 0.19 0.44
East Germany
Cases
212
0.17 0.40 0.6119
Controls 200
0.16 0.41
West Germany
combined
Cases
0.20 0.45 0.7644
W. Germany - ongoing
Cases
W. Germany - industrial
758
Controls 1036 0.20 0.45
381
0.22 0.47 0.8819
Controls 167
0.20 0.47
Cases
0.19 0.43 0.2880
377
Controls 869
0.20 0.44
hospitala
Cases
86
0.24 0.47 0.2518
DO - occupationala
Cases
291
0.17 0.42 0.0830
DO - controls
Controls 869
DO -
0.8643
0.1073
OR
95% CI
0.99
0.88 - 1.12
0.20 0.44
Non Caucasian study groups
Pakistan
Venezuela
All combined
Cases
98
0.23 0.43 0.9288
Controls 56
0.14 0.35
Cases
Controls
Cases
Controls
0.21
0.14
0.20
0.18
112
188
1402
1554
0.41 0.2428
0.39
0.44 0.3896
0.42
aControls
are the Dortmund control group
OR = odds ratio; 95% CI = 95% confidence intervals
P values of the Wald test, ORs and 95% CIs for the recessive (A/A vs. A/C, C/C) and for the
multiplicative ([A] vs. [C]) model, respectively, adjusted for age, gender and smoking habits are given.
N denotes the number of observations used for the multivariable logistic regression model. The
combined Caucasian study groups have been adjusted for different regions (Hungary, East Germany,
West Germany). All study groups combined have been adjusted for different ethnicities.
5
Supplemental Table S4. No effect of the rare [A] allele of the IGFBP3 SNP
rs2854744 on bladder cancer risk in subgroups of smokers and non-smokers nor
differences between smokers and non-smokers in rs2854744 distribution.
A. Frequencies of the genotypes and alleles of rs2854744 in non-smokers.
Genotype
Study groups
Status
N
C/C
Caucasians combined
Cases
259
85
0.33 124
0.48 50
0.19
0.57
0.43
Controls
587
192
0.33 282
0.48 113
0.19
0.57
0.43
Cases
61
20
0.33 32
0.52 9
0.15
0.59
0.41
Controls
34
14
0.41 14
0.41 6
0.18
0.62
0.38
Cases
198
65
0.33 92
0.46 41
0.21
0.56
0.44
Controls
553
178
0.32 268
0.48 107
0.19
0.56
0.44
Cases
50
17
0.34 25
0.50 8
0.16
0.59
0.41
Controls
93
34
0.37 40
0.43 19
0.20
0.58
0.42
Cases
148
48
0.32 67
0.45 33
0.22
0.55
0.45
Controls
460
144
0.31 228
0.50 88
0.19
0.56
0.44
Cases
70
22
0.31 35
0.50 13
0.19
0.56
0.44
Controls
62
18
0.29 33
0.53 11
0.18
0.56
0.44
Cases
78
26
0.33 32
0.41 20
0.26
0.54
0.46
Controls
398
126
0.32 195
0.49 77
0.19
0.56
0.44
DO - hospital
Cases
16
4
0.25 6
0.38 6
0.38
0.44
0.56
DO - occupational
Cases
62
22
0.35 26
0.42 14
0.23
0.56
0.44
DO - controls
Controls
398
126
0.32 195
0.49 77
0.19
0.56
0.44
Cases
33
11
0.33 11
0.33 11
0.33
0.50
0.50
Controls
8
1
0.13 4
0.50 3
0.38
0.38
0.63
Venezuela
Cases
13
3
0.23 5
0.38 5
0.38
0.42
0.58
All combined
Controls
Cases
118
305
50
99
0.42 53
0.32 140
0.45 15
0.46 66
0.13
0.22
0.65
0.55
0.35
0.45
Controls
713
243
0.34 339
0.48 131
0.18
0.58
0.42
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
A/C
Allele
A/A
[C]
[A]
Non Caucasian study group
Pakistan
6
B. Unadjusted P values, ORs, 95% CIs of rs2854744 in non-smokers.
Global Additive
Recessive
Study groups
Status
N
P value P value
P value
Caucasians combined
Cases
259
0.9494
0.7528
0.7718
Controls
587
Cases
61
0.5720
0.7096
0.7727
Controls
34
Cases
198
0.8721
0.9029
0.7549
Controls
553
Cases
50
0.6803
0.8821
0.6553
Controls
93
Cases
148
0.6000
0.7606
0.4088
Controls
460
Cases
70
0.9720
0.8958
1.0000
Controls
62
Cases
78
0.3403
0.6013
0.2197
Controls
398
DO - hospitala
Cases
16
0.2188
0.1695
0.1043
DO - occupationala
Cases
62
0.5831
0.9513
0.6069
DO - controls
Controls
398
Cases
33
0.5439
0.4248
1.0000
Controls
8
Cases
13
0.0427
0.0283
0.0288
Controls
118
Cases
305
0.8753
0.7215
0.6093
Controls
713
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
OR
95% CI
1.06
0.721.55
0.81
0.233.06
1.09
0.711.65
0.74
0.261.97
1.21
0.751.94
1.06
0.402.86
1.44
0.772.60
2.50
0.727.85
1.22
0.592.38
Multiplicative
P value
0.7504
0.7587
0.9061
0.9005
0.7879
0.9019
0.5981
0.2040
1.0000
OR
95% CI
1.04
0.831.29
1.12
0.592.16
1.01
0.801.29
0.96
0.571.62
1.04
0.791.37
0.97
0.581.62
1.10
0.771.57
1.65
0.763.63
0.99
0.661.47
Non Caucasian study groups
Pakistan
Venezuela
All combined
aControls
0.83
0.4148
0.136.39
4.29
0.0321
0.96 –
17.08
1.09
0.7174
0.771.54
0.60
0.162.09
2.51
1.026.33
1.04
0.851.26
are the Dortmund control group
OR = odds ratio; 95% CI = 95% confidence intervals
P values of the exact chi-square test for the genotype, recessive and multiplicative model, P values of
the Cochran-Armitage trend test for the additive model, ORs and 95% CIs for the recessive and of the
multiplicative model are given. The combined Caucasian study groups have been adjusted for
different regions (Hungary, East Germany, West Germany). All study groups combined have been
adjusted for different ethnicities.
Breslow-Day tests indicate the homogeneity of the ORs (recessive model: Caucasians P=0.5591, all
combined P=0.0633; multiplicative model: Caucasians P=0.9259, all combined P=0.0574).
7
C. Frequencies of the genotypes and alleles of rs2854744 in former smokers.
Genotype
Allele
Study groups
Status
N
C/C
A/C
A/A
Caucasians combined
Cases
581
165
0.28 299
0.51 117
0.20
0.54
0.46
Controls
503
154
0.31 252
0.50 97
0.19
0.56
0.44
Cases
80
16
0.20 42
0.53 22
0.28
0.46
0.54
Controls
26
8
0.31 16
0.62 2
0.08
0.62
0.38
Cases
501
149
0.30 257
0.51 95
0.19
0.55
0.45
Controls
477
146
0.31 236
0.49 95
0.20
0.55
0.45
Cases
103
38
0.37 47
0.46 18
0.17
0.60
0.40
Controls
88
27
0.31 50
0.57 11
0.13
0.59
0.41
Cases
398
111
0.28 210
0.53 77
0.19
0.54
0.46
Controls
389
119
0.31 186
0.48 84
0.22
0.54
0.46
Cases
220
57
0.26 113
0.51 50
0.23
0.52
0.48
Controls
68
19
0.28 37
0.54 12
0.18
0.55
0.45
Cases
178
54
0.30 97
0.54 27
0.15
0.58
0.42
Controls
321
100
0.31 149
0.46 72
0.22
0.54
0.46
DO - hospital
Cases
24
6
0.25 12
0.50 6
0.25
0.50
0.50
DO - occupational
Cases
154
48
0.31 85
0.55 21
0.14
0.59
0.41
DO - controls
Controls
321
100
0.31 149
0.46 72
0.22
0.54
0.46
Cases
0
-
-
-
-
-
-
-
-
Controls
0
-
-
-
-
-
-
-
-
Venezuela
Cases
42
14
0.33 18
0.43 10
0.24
0.55
0.45
All combined
Controls
Cases
35
623
7
179
0.20 20
0.29 317
0.57 8
0.51 127
0.23
0.20
0.49
0.54
0.51
0.46
Controls
538
161
0.30 272
0.51 105
0.20
0.55
0.45
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
[C]
[A]
Non Caucasian study groups
Pakistan
8
D. Unadjusted P values, ORs, 95% CIs of rs2854744 in former smokers.
Global Additive
Recessive
Study groups
Status
N
P value P value
P value
Caucasians combined
Cases
581
0.8292
0.5754
0.7771
Controls
503
Cases
80
0.1156
0.0441
0.0563
Controls
26
Cases
501
0.8533
0.9845
0.7466
Controls
477
Cases
103
0.2861
0.8998
0.9273
Controls
88
Cases
398
0.3815
0.9273
0.4797
Controls
389
Cases
220
0.6908
0.4579
0.4045
Controls
68
Cases
178
0.0999
0.3268
0.0606
Controls
321
DO - hospitala
Cases
24
0.8569
0.5701
0.8009
DO - occupationala
Cases
154
0.0565
0.2010
0.0261
DO - controls
Controls
321
Cases
0
-
-
Controls
0
Cases
42
0.3805
0.4465
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
Multiplicative
OR
95% CI
1.05
0.771.42
4.55
0.9842.51
0.94
0.681.31
1.48
0.623.70
0.87
0.611.25
1.37
0.663.04
0.62
0.371.03
1.15
0.363.17
0.55
0.310.95
P value
-
-
-
-
1.0000
1.05
0.32
3.55
1.06
0.791.41
0.5176
0.78
0.391.55
1.04
0.881.23
0.5808
0.0785
1.0000
0.9171
0.9596
0.4926
0.3520
0.6528
0.2096
OR
95% CI
1.05
0.881.25
1.86
0.943.73
1.00
0.831.20
0.97
0.631.50
1.01
0.821.24
1.15
0.771.73
0.88
0.671.15
1.19
0.632.24
0.84
0.631.11
Non Caucasian study groups
Pakistan
Venezuela
All combined
Controls
35
Cases
623
Controls
538
0.8816
aControls
0.6169
0.7150
0.6211
are the Dortmund control group
Breslow-Day tests indicate the homogeneity of the ORs (recessive model: All combined P= 0.9990,
Caucasians P=0.0525; multiplicative model: all combined P=0.3551, Caucasians P=0.1838).
9
E. Frequencies of the genotypes and alleles of rs2854744 in current smokers.
Genotype
Allele
Study groups
Status
N
C/C
A/C
Caucasians combined
Cases
353
117
0.33 172
0.49 64
0.18
0.58
0.42
Controls
222
62
0.28 118
0.53 42
0.19
0.55
0.45
Cases
82
24
0.29 44
0.54 14
0.17
0.56
0.44
Controls
15
2
0.13 10
0.67 3
0.20
0.47
0.53
Cases
271
93
0.34 128
0.47 50
0.18
0.58
0.42
Controls
207
60
0.29 108
0.52 39
0.19
0.55
0.45
Cases
59
23
0.39 27
0.46 9
0.15
0.62
0.38
Controls
19
6
0.32 11
0.58 2
0.11
0.61
0.39
Cases
212
70
0.33 101
0.48 41
0.19
0.57
0.43
Controls
188
54
0.29 97
0.52 37
0.20
0.55
0.45
Cases
91
28
0.31 43
0.47 20
0.22
0.54
0.46
Controls
37
7
0.19 20
0.54 10
0.27
0.46
0.54
Cases
121
42
0.35 58
0.48 21
0.17
0.59
0.41
Controls
151
47
0.31 77
0.51 27
0.18
0.57
0.43
DO - hospital
Cases
46
16
0.35 21
0.46 9
0.20
0.58
0.42
DO - occupational
Cases
75
26
0.35 37
0.49 12
0.16
0.59
0.41
DO - controls
Controls
151
47
0.31 77
0.51 27
0.18
0.57
0.43
Cases
65
25
0.38 28
0.43 12
0.18
0.60
0.40
Controls
48
19
0.40 22
0.46 7
0.15
0.63
0.38
Venezuela
Cases
57
28
0.49 20
0.35 9
0.16
0.67
0.33
All combined
Controls
Cases
35
475
12
170
0.34 19
0.36 220
0.54 4
0.46 85
0.11
0.18
0.61
0.59
0.39
0.41
Controls
305
93
0.30 159
0.52 53
0.17
0.57
0.43
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
A/A
[C]
[A]
Non Caucasian study groups
Pakistan
10
F. Unadjusted P values, ORs, 95% CIs of rs2854744 in current smokers.
Global Additive
Recessive
Study groups
Status
N
P value P value
P value
Caucasians combined
Cases
353
0.3309
0.3444
0.9943
Controls
222
Cases
82
0.4391
0.3083
1.0000
Controls
15
Cases
271
0.4549
0.3750
1.0000
Controls
207
Cases
59
0.7129
0.8810
0.7238
Controls
19
Cases
212
0.6435
0.5093
1.0000
Controls
188
Cases
91
0.3911
0.2232
0.6458
Controls
37
Cases
121
0.8233
0.6271
1.0000
Controls
151
DO - hospitala
Cases
46
0.8337
0.8663
0.8285
DO - occupationala
Cases
75
0.8611
0.5768
0.8522
DO - controls
Controls
151
Cases
65
0.8772
0.7126
0.6218
Controls
48
Cases
57
0.2280
0.4858
0.7598
Controls
35
Cases
475
0.2264
0.3390
0.8564
Controls
305
Hungary
Germany combined
East Germany
West Germany combined:
W. Germany - ongoing
W. Germany - industrial
Multiplicative
OR
95% CI
1.00
0.641.56
0.82
0.195.14
0.97
0.601.60
1.53
0.2715.86
0.98
0.581.66
0.76
0.292.07
0.96
0.491.89
1.12
0.422.72
0.87
0.381.93
P value
1.33
0.434.34
1.45
0.367.00
1.04
0.711.51
0.7828
0.3491
0.4256
0.3922
1.0000
0.5216
0.2697
0.6629
0.9047
0.6142
OR
95% CI
0.89
0.691.14
0.68
0.291.61
0.89
0.681.16
0.95
0.422.17
0.91
0.681.22
0.71
0.401.27
0.92
0.641.31
0.96
0.581.58
0.89
0.591.36
Non Caucasian study groups
Pakistan
Venezuela
All combined
aControls
0.5262
0.3394
1.11
0.621.98
0.80
0.411.56
0.90
0.741.11
are the Dortmund control group
Breslow-Day tests indicate the homogeneity of the ORs (recessive model: All combined P=0.7176,
Caucasians P=0.8405; multiplicative model: all combined P=0.6880, Caucasians P=0.7851).
11
G. Frequencies of the genotypes and alleles of rs2854744 in ever smokers (current
and former smokers).
Genotype
Allele
Study groups
Status
N
C/C
A/C
A/A
Caucasians combined
Cases
934
282
0.30 471
0.50 181
0.19
0.55
0.45
Controls
725
216
0.30 370
0.51 139
0.19
0.55
0.45
Cases
162
40
0.25 86
0.53 36
0.22
0.51
0.49
Controls
41
10
0.24 26
0.63 5
0.12
0.56
0.44
Cases
772
242
0.31 385
0.50 145
0.19
0.56
0.44
Controls
684
206
0.30 344
0.50 134
0.20
0.55
0.45
Cases
162
61
0.38 74
0.46 27
0.17
0.60
0.40
Controls
107
33
0.31 61
0.57 13
0.12
0.59
0.41
Cases
610
181
0.30 311
0.51 118
0.19
0.55
0.45
Controls
577
173
0.30 283
0.49 121
0.21
0.55
0.45
Cases
311
85
0.27 156
0.50 70
0.23
0.52
0.48
Controls
105
26
0.25 57
0.54 22
0.21
0.52
0.48
Cases
299
96
0.32 155
0.52 48
0.16
0.58
0.42
Controls
472
147
0.31 226
0.48 99
0.21
0.55
0.45
DO - hospital
Cases
70
22
0.31 33
0.47 15
0.21
0.55
0.45
DO - occupational
Cases
229
74
0.32 122
0.53 33
0.14
0.59
0.41
DO - controls
Controls
472
147
0.31 226
0.48 99
0.21
0.55
0.45
Cases
65
25
0.38 28
0.43 12
0.18
0.60
0.40
Controls
48
19
0.40 22
0.46 7
0.15
0.63
0.38
Venezuela
Cases
99
42
0.42 38
0.38 19
0.19
0.62
0.38
All combined
Controls
Cases
70
19
1098 349
0.27 39
0.32 537
0.56 12
0.49 212
0.17
0.19
0.55
0.56
0.45
0.44
Controls
843
0.30 431
0.51 158
0.19
0.56
0.44
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
[C]
[A]
Non Caucasian study groups
Pakistan
254
12
H. Unadjusted P values, ORs, 95% CIs of rs2854744 in ever smokers (current and
former smokers).
Global Additive
Recessive
Multiplicative
Study groups
Status
N
P value P value
P value
P value
OR
95% CI
OR
95% CI
Caucasians combined
Cases
934
0.8807
0.8731
0.8611
0.8747
Controls
725
Cases
162
0.3409
0.4050
0.1934
Controls
41
Cases
772
0.8538
0.5776
0.7388
Controls
684
Cases
162
0.1847
0.7853
0.3821
Controls
107
Cases
610
0.7296
0.7462
0.5148
Controls
577
Cases
311
0.7839
0.8975
0.7870
Controls
105
Cases
299
0.2276
0.2556
0.0915
Controls
472
DO - hospitala
Cases
70
1.0000
0.9852
1.0000
DO - occupationala
Cases
229
0.1045
0.1689
0.0395
1.02
0.791.32
2.06
0.737.19
0.95
0.721.24
1.45
0.683.22
0.90
0.671.21
1.10
0.621.98
0.72
0.481.07
1.03
0.521.94
0.63
0.400.99
0.99
0.861.14
1.22
0.732.05
0.96
0.831.11
0.95
0.661.38
0.97
0.831.15
0.98
0.711.36
0.89
0.721.10
1.00
0.691.46
0.85
0.681.08
DO - controls
Controls
472
Cases
65
0.8772
0.7126
0.6218
0.7828
Controls
48
Cases
Controls
99
0.0658
0.2367
0.8410
Cases
1098 0.6305
0.7508
0.7483
Controls
843
1.33
0.434.34
1.15
0.482.81
1.04
0.831.31
Hungary
Germany combined
East Germany
West Germany combined
W. Germany - ongoing
W. Germany - industrial
0.4594
0.6007
0.8573
0.7727
0.9364
0.2688
1.0000
0.1867
Non Caucasian study groups
Pakistan
Venezuela
All combined
70
aControls
0.2619
0.7525
1.11
0.621.98
0.76
0.481.21
0.98
0.861.11
are the Dortmund control group
Breslow-Day tests indicate the homogeneity of the ORs (recessive model: All combined P=0.8516,
Caucasians P=0.1690; multiplicative model: all combined P= 0.4652, Caucasians P= 0.6806).
13
I. No difference in the distribution of rs2854744 A/A genotypes between smokers and
non-smokers.
Study groups
Caucasians combined
Hungary
Germany combined
Status
Smoking habits N
Cases
Smokers
Non-smokers
Controls Smokers
Non-smokers
Cases
Smokers
Non-smokers
Controls Smokers
Non-smokers
Cases
Smokers
Non-smokers
934
259
725
587
162
61
41
34
772
198
Genotype
C/C A/C
0.30 0.50
0.33 0.48
0.30 0.51
0.33 0.48
0.25 0.53
0.33 0.52
0.24 0.63
0.41 0.41
0.31 0.50
0.33 0.46
A/A
0.19
0.19
0.19
0.19
0.22
0.15
0.12
0.18
0.19
0.21
Global P value
(recessive model)
0.6776
(0.9933)1
0.4823
(0.9524)2
0.3281
(0.2633)
0.1905
(0.5317)
0.6769
(0.5445)
Controls Smokers
684 0.30 0.50 0.20 0.7273
Non-smokers
553 0.32 0.48 0.19 (0.9425)
East Germany
Cases
Smokers
162 0.38 0.46 0.17 0.8441
Non-smokers
50
0.34 0.50 0.16 (1.0000)
Controls Smokers
107 0.31 0.57 0.12 0.1015
Non-smokers
93
0.37 0.43 0.20 (0.1249)
West Germany combined Cases
Smokers
610 0.30 0.51 0.19 0.4603
Non-smokers
148 0.32 0.45 0.22 (0.4232)
Controls Smokers
577 0.30 0.49 0.21 0.7441
Non-smokers
460 0.31 0.50 0.19 (0.4838)
W. Germany - ongoing
Cases
Smokers
311 0.27 0.50 0.23 0.6744
Non-smokers
70
0.31 0.50 0.19 (0.5249)
Controls Smokers
105 0.25 0.54 0.21 0.8050
(0.6904)
Non-smokers
62
0.29 0.53 18
W. Germany - industrial
Cases
Smokers
299 0.32 0.52 0.16 0.0943
Non-smokers
78
0.33 0.41 0.26 (0.0677)
Controls Smokers
472 0.31 0.48 0.21 0.8456
Non-smokers
398 0.32 0.49 0.19 (0.5548)
DO - hospital
Cases
Smokers
70
0.31 0.47 0.21 0.4061
Non-smokers
16
0.25 0.38 0.38 (0.2038)
DO - occupational
Cases
Smokers
229 0.32 0.53 0.14 0.1860
Non-smokers
62
0.35 0.42 0.23 (0.1717)
DO - controls
Controls Smokers
472 0.31 0.48 0.21 0.8456
Non-smokers
398 0.32 0.49 0.19 (0.5548)
Non Caucasian study groups
Pakistan
Cases
Smokers
65
0.38 0.43 0.18 0.2780
Non-smokers
33
0.33 0.33 0.33 (0.1311)
Controls Smokers
48
0.40 0.46 0.15 0.1832
Non-smokers
8
0.13 0.50 0.38 (0.1428)
Venezuela
Cases
Smokers
99
0.42 0.38 0.19 0.2389
Non-smokers
13
0.23 0.38 0.38 (0.1474)
Controls Smokers
70
0.27 0.56 0.17 0.1209
Non-smokers
118 0.42 0.45 0.13 (0.5192)
All combined
Cases
Smokers
1098 0.32 0.49 0.19 0.5700
Non-smokers
305 0.32 0.46 0.22 (0.3857)3
Controls Smokers
843 0.30 0.51 0.19 0.2481
Non-smokers
713 34
0.48 0.18 (0.9871)4
P values of the exact chi-square test for global differences between ever smokers and non
smokers are given. P values of the exact chi-square test for a different A/A distribution between
ever smokers and non smokers are given in parenthesis. P values for combined Caucasian
groups adjusted for region and P values for all study groups combined adjusted for ethnicity were
calculated using the method of Mantel-Haenszel.
14
1Breslow-Day
test for homogeneity of the ORs in the Caucasian case group P=0.3331
test for homogeneity of the ORs in the Caucasian case group P=0.1726
3Breslow-Day test for homogeneity of the ORs in the case group P=0.1163
4Breslow-Day test for homogeneity of the ORs in the case group P=0.2060
2Breslow-Day
15
Supplemental Table S5. No association between rs2854744 and urinary bladder
cancer carcinogens.
A. No difference in the distribution of rs2854744 between individuals exposed and
non exposed to urinary bladder carcinogens.
Exposure
Genotype
Study groups
Status
All combined
Cases
PAH
East
Germany
W. Germany
occupational
All combined
AA
East
Germany
W. Germany
occupational
W. Germany
- hospital
All combined
exposed
not
exposed
Cases
exposed
not
exposed
Controls exposed
74
299
30
99
0.41
0.33
29
154
0.39
0.52
15
46
0.20
0.15
0.40
0.41
18
169
9
63
0.50
0.37
6
79
0.33
0.47
3
27
0.17
0.16
0.33
0.39
2
1
0.50
0
0.00
1
0.50
0.50
not
exposed
exposed
196
65
0.33
100
0.51
31
0.16
0.41
56
21
0.38
23
0.41
12
0.21
0.42
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Controls exposed
not
exposed
Cases
exposed
130
36
0.28
75
0.58
19
0.15
0.43
131
328
40
115
0.31
0.35
67
155
0.51
0.47
24
58
0.18
0.18
0.44
0.41
13
175
3
69
0.23
0.39
6
79
0.46
0.45
4
27
0.31
0.15
0.54
0.38
2
196
1
65
0.50
0.33
0
100
0.00
0.51
1
31
0.50
0.16
0.50
0.41
118
37
0.31
61
0.52
20
0.17
0.43
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Controls exposed
68
20
0.29
37
0.54
11
0.16
0.43
0
85
26
0.31
39
0.46
20
0.24
0.46
165
302
56
98
0.34
0.32
82
147
0.50
0.49
27
57
0.16
0.19
0.41
0.43
34
162
16
55
0.47
0.34
13
79
0.38
0.49
5
28
0.15
0.17
0.34
0.42
PAH or
AA
W. Germany
occupational
Cases
W. Germany
- hospital
Cases
All combined
Cases
East
Cases
A/A
0.0657
(0.3156;
0.5469)
0.5076
(1.0000;
0.5902)
-
C/C
AD
East
Germany
A/C
Global P
value
(recessive
model;
multiplicati
ve model)
N
Cases
Exposure
Freq.
[A]
0.1137
(0.2856;
0.8201)
0.7296
(0.4300;
0.5215)
0.3146
(0.2344;
0.1440)
-
0.9362
(1.0000;
0.9139)
-
0.6868
(0.7531;
0.4520)
0.3713
(0.8066;
0.2769)
22
9
0.41
10
0.45
3
0.14
0.36
not
exposed
exposed
175
58
0.33
89
0.51
28
0.16
0.41
124
37
0.30
66
0.53
21
0.17
0.44
not
exposed
exposed
not
exposed
exposed
not
exposed
exposed
62
20
0.32
32
0.52
10
0.16
0.42
7
78
3
23
0.43
0.29
3
36
0.43
0.46
1
19
0.14
0.24
0.36
0.47
-
177
196
60
69
0.34
0.35
85
98
0.48
0.50
32
29
0.18
0.15
0.42
0.40
31
12
0.39
12
0.39
7
0.23
0.42
0.2709
(0.3375;
0.9050)
0.5424
16
0.7871
(1.0000;
0.6260)
0.9551
(1.0000;
0.8244)
Germany
PAH, AA or AD
W. Germany
occupational
All combined
East
Germany
PAH or ever smoking
W. Germany
occupational
All combined
East
Germany
W. Germany
occupational
not
exposed
Controls exposed
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Controls exposed
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Cases
exposed
not
exposed
Controls exposed
not
exposed
Cases
exposed
156
60
0.38
73
0.47
23
0.15
0.38
2
196
1
65
0.50
0.33
0
100
0.00
0.51
1
31
0.50
0.16
0.50
0.41
146
40
48
9
0.33
0.23
73
25
0.50
0.63
25
6
0.17
0.15
0.42
0.46
230
141
77
48
0.33
0.34
113
71
0.49
0.50
40
22
0.17
0.16
0.42
0.41
60
125
25
43
0.42
0.34
24
62
0.40
0.50
11
20
0.18
0.16
0.38
0.41
23
173
9
57
0.39
0.33
10
89
0.43
0.51
4
27
0.17
0.16
0.39
0.41
170
16
52
5
0.31
0.31
89
9
0.52
0.56
29
2
0.17
0.13
0.43
0.41
408
66
144
20
0.35
0.30
199
33
0.49
0.50
65
13
0.16
0.20
0.40
0.45
168
39
65
12
0.39
0.31
75
20
0.45
0.51
28
7
0.17
0.18
0.39
0.44
107
93
33
34
0.31
0.37
61
40
0.57
0.43
13
19
0.12
0.20
0.41
0.42
240
79
0.33
124
0.52
37
0.15
0.41
(0.2882;
0.6688)
-
0.3647
(0.8164;
0.5260)
0.4851
(0.5618;
0.8109)
0.5124
(0.8338;
0.7339)
0.7897
(1.0000;
0.8736)
0.9413
(0.7460;
0.8530)
0.5868
(0.4602;
0.3024)
0.6448
(1.0000;
0.5212)
0.1015
(0.1249;
0.8388)
0.6766
(0.4054;
0.5608)
PAH. AA, AD or
ever smoking
PAH, AA or
ever smoking
not
27
8
0.30 13
0.48 6
0.22 0.46
exposed
All combined Cases
exposed
426 150 0.35 206 0.48 70
0.16 0.41
0.5102
(0.9908;
not
48
14
0.29 26
0.54 8
0.17 0.44
0.4549)
exposed
East
Cases
exposed
170 65
0.38 76
0.45 29
0.17 0.39
0.7623
Germany
(1.0000;
not
37
12
0.32 19
0.51 6
0.16 0.42
0.6963)
exposed
Controls exposed
107 33
0.31 61
0.57 13
0.12 0.41
0.1015
(0.1249;
not
93
34
0.37 40
0.43 19
0.20 0.42
0.8388)
exposed
W. Germany Cases
exposed
256 85
0.33 130 0.51 41
0.16 0.41
0.5891
(1.0000;
not
11
2
0.18 7
0.64 2
0.18 0.50
occupational
0.5092)
exposed
All combined Cases
exposed
437 152 0.35 213 0.49 72
0.16 0.41
0.6109
(0.9155;
not
37
11
0.30 20
0.54 6
0.16 0.43
0.5743)
exposed
East
Cases
exposed
174 66
0.38 79
0.45 29
0.17 0.39
0.7194
Germany
(1.0000;
not
33
10
0.30 17
0.52 6
0.18 0.44
0.4963)
exposed
Controls exposed
118 37
0.31 65
0.55 16
0.14 0.41
0.2763
(0.3271;
not
82
30
0.37 36
0.44 16
0.20 0.41
1.0000)
exposed
W. Germany Cases
exposed
263 86
0.33 134 0.51 43
0.16 0.42
not
4
1
0.25 3
0.48 0
0.00 0.38
occupational
exposed
PAH: polycyclic aromatic hydrocarbons, AA: aromatic amines, AD: azo dyes
Freq.: Frequency
P values of the exact chi-square test for global differences between exposed and non exposed persons are
given. P values of the exact chi-square test for a different A/A distribution between exposed and not exposed
persons (recessive model) as well as P values of the exact chi-square test of a different allele distribution
(multiplicative model) are given in parenthesis. P values for combined case groups were calculated using the
method of Mantel-Haenszel.
17
B. No differences between cases and controls in the subgroups of exposed and not
PAH
Exposure
exposed persons.
Study groups
All combined
(East
Germany,
W.Germany –
occupational)
exposed
not
exposed
exposed
East Germany
AA
Exposure
All combined
(East
Germany, W.
Germany –
occupational,
W. Germany –
hospital)
not
exposed
exposed
not
exposed
exposed
East Germany
AD
not
exposed
All combined
(East
Germany, W.
Germany –
occupational,
W. Germany –
hospital)
Status
N
Global
A/A
Recessive Multiplicative
[A] allele P value
genotype
model
model
frequency (additive
frequency
P value
P value
model)
Cases
74
Controls 2
Cases 299
Controls 196
0.20
0.50
0.15
0.16
0.40
0.50
0.41
0.41
Cases
18
Controls 2
Cases 169
0.17
0.50
0.16
0.33
0.50
0.39
Controls 196
0.16
0.41
Cases
131
0.18
0.44
Controls
2
0.50
0.50
Cases
328
0.18
0.41
Controls
31
0.16
0.41
Cases
13
0.31
0.54
Controls
2
0.50
0.50
Cases
175
0.15
0.38
Controls 196
0.16
0.41
Cases
165
0.16
0.41
Controls
22
0.14
0.36
Cases
302
0.19
0.43
Controls 175
0.16
0.41
exposed
not
exposed
Cases
34
0.15
0.34
Controls
22
0.14
0.36
Cases
162
0.17
0.42
Controls 175
0.16
0.41
exposed
-
-
-
0.9918
(1.0000)
0.8997
1.0000
-
-
-
0.6846
(0.5945)
1.0000
0.5973
-
-
-
0.6976
(1.0000)
0.6316
1.0000
-
-
-
0.4406
(0.3669)
1.0000
0.3680
0.8236
(0.6226)
0.7780
0.6250
0.7387
(0.6328)
0.4585
0.6344
0.9302
(0.8490)
1.0000
0.8404
0.9242
(1.0000)
0.7714
1.0000
-
-
-
0.8966
(0.7099)
0.8886
0.7161
-
-
-
0.5865
(0.3903)
0.8818
0.3955
0.9163
(0.7564)
1.0000
0.7558
0.9875
(0.9334)
1.0000
0.9350
East Germany
PAH or AA
not
exposed
All combined
(East
Germany,
W.Germany –
occupational)
exposed
not
exposed
exposed
East Germany
PAH, AA or AD
not
exposed
All combined
(East
Germany,
W.Germany –
occupational)
exposed
not
exposed
exposed
East Germany
not
Cases
177
0.18
0.42
Controls
2
0.50
0.50
Cases 196
Controls 196
Cases
31
0.15
0.16
0.23
0.40
0.41
0.42
Controls
2
0.50
0.50
Cases
156
0.15
0.38
Controls 196
0.16
0.41
Cases 230
Controls 23
Cases 141
0.17
0.17
0.16
0.42
0.39
0.41
Controls 173
Cases
60
0.16
0.18
0.41
0.38
Controls
23
0.17
0.39
1.0000
(1.0000)
1.0000
1.0000
Cases
125
0.16
0.41
0.9482
1.0000
0.9328
18
PAH, AA, AD or
ever smoking
PAH, AA or
ever smoking
PAH or ever smoking
exposed
All combined
(East
Germany,
W.Germany –
occupational)
exposed
not
exposed
exposed
East Germany
All combined
(East
Germany,
W.Germany –
occupational)
not
exposed
exposed
not
exposed
exposed
East Germany
All combined
(East
Germany,
W.Germany –
occupational)
not
exposed
exposed
not
exposed
exposed
East Germany
not
exposed
(0.9314)
Controls 173
0.16
0.41
Cases 408
Controls 107
0.16
0.12
0.40
0.41
0.2946
(0.9363)
0.3671
0.9377
0.6322
(0.6581)
1.0000
0.6470
0.3857
0.7211
0.8148
0.8915
0.2538
(1.0000)
0.3006
1.0000
0.4789
(0.8050)
0.6572
0.8001
0.1351
(0.7869)
0.3049
0.7897
0.6922
(1.0000)
0.6328
1.0000
0.4690
(1.0000)
0.4810
1.0000
0.6106
(0.8897)
0.8010
0.8873
0.2821
(0.7278)
0.5123
0.7308
0.7684
(0.7760)
1.0000
0.7689
Cases
66
0.20
0.45
Controls
Cases
Controls
Cases
Controls
Cases
Controls
Cases
93
168
107
39
93
426
107
48
0.20
0.17
0.12
0.18
0.20
0.16
0.12
0.17
0.42
0.39
0.41
0.44
0.42
0.41
0.41
0.44
Controls
93
0.20
0.42
Cases 170
Controls 107
Cases
37
0.17
0.12
0.16
0.39
0.41
0.42
Controls
93
0.20
0.42
Cases 437
Controls 118
Cases
82
0.16
0.14
0.20
0.41
0.41
0.43
Controls
37
0.16
0.41
Cases 174
Controls 118
Cases
33
0.17
0.14
0.18
0.39
0.41
0.44
Controls
0.20
0.41
82
0.1384
(0.7171)
0.6795
(0.8956)
PAH: polycyclic aromatic hydrocarbons, AA: aromatic amines, AD: azo dyes
P values of the exact chi-square test for global differences between cases and controls. Exact P values of the
Cochran-Armitage trend test (additive model) are given in parenthesis. P values of the exact chi-square test for a
different A/A distribution between cases and controls (recessive model) as well as P values of the exact chisquare test of a different allele distribution (multiplicative model).
19
Supplemental Table S6. Study group characteristics
A. Distribution of gender in the study groups.
Study groups
P value
Frequency males Frequency males
cases
N
controls
%
N
%
Caucasians combined
5.540E-28 991
0.80
791
0.60
Hungary
0.0573
146
0.60
56
0.73
Germany combined
6.368E-40 845
0.85
735
0.59
East Germany
1.0000
182
0.86
171
0.86
West Germany combined
9.053E-43 663
0.84
564
0.54
West Germany – ongoing
0.1584
302
0.78
121
0.72
West Germany – industrial 1.438E-42 361
0.91
443
0.51
DO - hospitala
1.518E-09 73
0.84
DO - occupationala
2.264E-38 288
0.93
443
0.51
DO - controls
Non Caucasian study groups
Pakistan
0.2066
85
0.87
95
0.80
Venezuela
8.065E-07 79
0.71
77
0.41
All combined
2.668E-34 1155
0.80
963
0.60
For 101 persons gender was unknown.
P value of the exact chi-square test
20
B. Distribution of age at diagnosis (cases) and at examination (controls), respectively,
in the study groups.
Study groups
Status
Caucasians combined
Cases
Hungary
Germany combined
East Germany
West Germany combined
West Germany – ongoing
N
Min
Max Median Mean Standard P value
deviation
1233 20.1 95.2 67.1
66.3 11.5
0.7039
Controls 1315 20.2 99.1 67.6
65.1
13.3
Cases
27.2 95.1 70.5
69.0
11.2
Controls 76
30.0 84.2 63.5
63.4
12.7
Cases
20.1 95.2 66.2
65.6
11.5
Controls 1239 20.2 99.1 67.7
6.52
13.4
Cases
240
993
212
20.1 91.3 65.6
65.0
11.2
Controls 200
29.4 91.2 67.7
67.5
10.2
Cases
26.1 95.2 66.3
65.8
11.5
Controls 1039 20.2 99.1 67.7
64.8
13.8
Cases
781
0.0022
0.1989
0.0201
0.7845
386
26.1 95.2 71.4
69.7
11.1
Controls 167
21.7 99.1 66.9
64.4
15.4
Cases
395
32.1 84.4 61.9
61.9
10.6
Controls 872
20.2 93.9 67.8
64.9
13.5
Dortmund - hospital
Cases
86
44.8 83.9 67.8
67.0
9.4
0.5600
Dortmund - occupational
Cases
309
32.1 84.4 60.8
60.5
10.5
<0.0001
Dortmund controls
Controls 872
20.2 93.9 67.8
64.9
13.5
24.0 82.0 60.5
59.1
13.3
26.0 76.0 56.0
54.1
13.9
Cases
112 29.3 87.1 59.1
Controls 188 20.0 90.5 30.8
Cases
1443 20.1 95.2 66.2
60.2
36.3
65.3
11.8
15.6
11.9
Controls 1622 20.0 99.1 65.3
61.0
16.6
West Germany - industrial
0.0002
<0.0001
Non Caucasian study groups
Pakistan
Cases
98
Controls 119
Venezuela
All combined
For 7 cases and 103 controls age was unknown.
P value of the Wilcoxon rank sum test.
21
0.0145
<0.0001
<0.0001
C. Frequency of non smokers, former smokers and current smokers in the study
groups.
Smoking habits
Study groups
Status
Caucasians combined
Cases
1193
Controls 1312
0.22
0.45
0.49
0.38
0.30
0.17
Hungary
Cases
0.27
0.36
0.37
Controls 75
0.45
0.35
0.20
Cases
0.20
0.52
0.28
Controls 1237
0.45
0.39
0.17
Cases
212
0.24
0.49
0.28
Controls 200
0.47
0.44
0.10
Cases
0.20
0.53
0.28
Controls 1037
0.44
0.38
0.18
Cases
381
0.18
0.58
0.24
Controls 167
0.37
0.41
0.22
Cases
377
0.21
0.47
0.32
Controls 870
0.46
0.37
0.17
Dortmund – hospital
Cases
86
0.19
0.28
0.53
Dortmund – occupational
Cases
291
0.21
0.53
0.26
Dortmund controls
Controls 870
0.46
0.37
0.17
98
0.34
0.00
0.66
Controls 56
0.14
0.00
0.86
Cases
Controls
Cases
Controls
0.12
0.63
0.22
0.46
0.38
0.19
0.44
0.35
0.51
0.19
0.34
0.20
Germany combined
East Germany
West Germany combined
West Germany – ongoing
West Germany – industrial
N
Non smokers Former smokers Current smokers
223
970
758
Non Caucasian study groups:
Pakistan
Venezuela
All combined
Cases
112
188
1403
1556
For 216 subjects smoking habits were unknown.
22