Supplementary Information
Supplementary Figure S1
Supplementary Fig. S1. PCR identification of Os04g54340 and Os08g08030 in rice.
Lane1, DNA marker; Lane2-4, PCR amplification of Os08g08030-F/R in Zhongxian
3037, Yandao 8 cDNA and water; Lane5-7, PCR amplification of Os04g54340-F/R in
Zhongxian 3037, Yandao 8 cDNA and water; 8-10: PCR amplification of Ubiquitin in
Zhongxian 3037, Yandao 8 cDNA and water.
Supplementary Figure S2
Supplementary Fig. S2. Characterization of the OsMRE11-deficient plant phenotype.
(a-b) Comparison of a wild-type plant (a) and an OsMRE11-deficient plant (b);
Fertile pollen grains stained with 1% I2-KI solution in a wild-type plant (c) and
completely sterile pollen grains in OsMRE11-deficient plants (d). Scale bars: 50 μm.
Supplementary Figure S3
Supplementary Fig. S3. Real-time PCR analysis of OsMRE11 in different tissues.
Ubiquitin is used as endogenous control. Error bars represent SD (n=3).
Supplementary Figure S4
Supplementary Fig. S4. Immunofluorescent localization of γH2AX on meiotic
spreads of the wild type and OsSPO11RNAi-deficient chromosomes.
Immunofluorescent localization of γH2AX on meiotic spreads of the wild type (a) and
OsSPO11RNAi-deficient chromosomes (b), The anti-OsREC8 antibody is used to
indicate the PMCs in green (the left panel) and the anti-γH2AX staining was shown in
red (the middle panel), the overlapped images are showed in the right panel. Scale
bars: 5 μm.
Supplementary Table S1. List of primers used in this study
Purpose
Name
Forward
Reverse
Gene
OS08G08030-F/R
ATGCATGCTAGCTTTGCATCA
TCAGATAACAGGAGCTTTGCC
Identificati
Os04g54340-F/R
ATGGGAGACGAAAGCAACACA
TCATCTCCTCCTAACAGCTCC
on
Mre11-F
GTGCAATCTTCTTCAGATGAG
pCAMB2300-R
CGACGTTGTAAAACGACGGCC
OsMRE11-RT-F/R
ATGGGAGACGAAAGCAACACA
GCACAAAATCAACCTTATTCT
Ubi-RT-F/R
CAAGATGATCTGCCGCAAATGC
TTTAACCAGTCCATGAACCCG
Antibody
OsMRE11-Ab-F/R
CAGGATCCATGGGAGACGAAAGCAACACA
GAGTCGACTCTCCTCCTAACAGCTCCGTA
RNAi
OsMRE11-RANi-F/R
GTGGATCCGTTACAGCTCAAAGTAACCTG
CAGTCGACTCTCCTCCTAACAGCTCCGTA
q-RT-PCR