ZOOL 409
and
Lab Week 10
 Slide 33 -- soft palate . Look for respiratory epithelium ( ciliated pseudostratified columnar epithelium) on the nasal surface of the palate.
Glands may also be present.
The quiz slides for lung represent post mortem human material. Quality of cell / tissue preservation is somewhat poor, but representative of real-world clinical autopsy specimens. All of the listed features should be readily identifiable, in spite of less-than-ideal preservation .
 Slides 36, 57, 58 -- trachea . Note ciliated pseudostratified columnar epithelium (goblet cells should be present but may be hard to find), cartilage. You may find glands beneath the epithelium, and there may be smooth muscle between the ends of the cartilage rings. Some specimens include a conspicuous venous plexus
(numerous large blood vessels, between mucosa and cartilage).
 Slides 59, 60, 61 -- lung . Note arrangement of alveoli . See (or imagine) the tissue elements
(simple squamous epithelium, capillaries) comprising the alveolar wall . Look for " dust cells " (alveolar macrophages, with ingested particulate matter), and other evidence of dirt or soot from inhaled air. Look for bronchi and bronchioles with associated smooth muscle.
Note that blood vessels (veins and arteries) of pulmonary circulation have more delicate walls than those of systemic circulation.
Note: As always, you are also encouraged to seek confirmation for your recognition of structures on slides from your reference slide set -- particularly of any features not included in these quizzes.
□
Lung:
____ Bronchus, with:
____ ciliated, columnar epithelium
____ cartilage
____ gland
____ smooth muscle
____ Bronchiole, with:
____ ciliated, cuboidal epithelium
____ Respiratory (gas exchange) tissue
____ alveoli
____ interalveolar septa w/ capillaries
____ Blood vessels
For our next two labs, bring to class the
"souvenir" slide you received during our histotechniques field trip.
Among several other organs ( which you should eventually be able to recognize ), this slide includes a nice specimen of kidney . Some details may show up better on this slide.
Last updated: 13 Dec. 2011 / dgk

ZOOL 409
and
Lab Week 10
Slides 62, 63, 64, also Slide from Ms. Doran --
There's a LOT going on, histologically, in the kidney. Begin by finding the basic regions.
In the renal cortex , try to distinguish proximal from distal tubules. Because each proximal convoluted tubules is much longer than the associated distal convoluted tubule, proximal tubules appear to be much more plentiful in the cortex. Proximal tubules are generally a bit larger in outside diameter, and their epithelial cells are more eosinophilic and a bit larger than distal tubule epithelial cells.
(These distinctions will not be evaluated.)
Identify the principal regions of kidney.
 Capsule -- the outermost layer of connective tissue, enveloping the kidney (often missing from dissected specimens).
 Cortex -- the outer region of kidney, consisting of convoluted tubules with scattered renal corpuscles .
 Medulla -- the deeper region of kidney, consisting of parallel tubules comprising loops of Henle and collecting ducts .
 Pelvis -- the "drain" of the kidney, which funnels urine from many tubules (collecting ducts) into the ureter. Transitional epithelium marks the beginning of the ureter .
Fat and large blood vessels may be found in the pelvis.
As you look for fine details (below), keep in mind how all of the details are organized into nephrons .
In the cortex , find renal corpuscles .
Distinguish the glomerulus from Bowman's capsule .
 Try to visualize Bowman's space and glomerular capillaries . Try to visualize (or at least to imagine) three kinds of cells in the glomerulus: o capillary endothelium o podocytes o mesangial cells
 Look for corpuscles cut in such a way that you can distinguish: o vascular pole (where the glomerulus is attached); o macula densa ; o urinary pole (where urinary space is drains into the proximal convoluted tubule ).
In the medulla, distinguish loops of Henle from collecting ducts . Collecting ducts are lined by pale cuboidal cells with distinct boundaries between cells.
Cuboidal epithelial cells lining thick segments of the loop of
Henle are more eosinophilic, with indistinct boundaries. Thin segments of the loops are lined by squamous cells with bulging nuclei. (These distinctions will not be evaluated.)
In both cortex and medulla, note (or imagine) renal stroma (especially peritubular capillaries and vasa recta ), in the very-thin regions in between the tubules.
Slides 65, 66 -- bladder and ureter. Note transitional epithelium , smooth muscle, connective tissue.
(A ureter is also included on slide 62 in Boxes 1, 2, 3, 6, 8, 9.)
The quiz slides for kidney represent post mortem human material, with less-than-ideal tissue preservation.
□
On slide of kidney
____ Glomerulus in cortex
____ Convoluted tubules in cortex
____ Collecting duct in medulla
____ Transitional epithelium of pelvis
□
On micrograph of glomerulus
____ Bowman's space
____ Podocyte
____ Capillary lumen
____ Filtration membrane
____ Macula densa
Last updated: 3 April 2013 / dgk