ZOOL 409
Lab Week 3
THURSDAY
Objectives:
Distinguish several varieties of connective tissue (CT).
Recognize the common components of connective tissue (cells and fibers).
Secondary objective: Review epithelial tissue as often as it appears.
______________________________________________________________________
Find ordinary connective tissue in several of
the following slides. Notice whether each
occurrence of connective tissue is relatively:
 dense -- mostly collagen fibers.
 loose -- lots of ground substance (clear area).
 cellular -- lots of nuclei (mostly lymphocytes).
 adipose -- lots of adipocytes.
Also note blood vessels passing through the CT.
_______________________________________
In skin, distinguish the relatively dense fibrous
CT of dermis from deeper adipose CT of
hypodermis (subcutaneous tissue).
 Skin -- slides 26, 27, 28.
_______________________________________
In the following "solid" (parenchymal) organs,
CT forms a stroma of thin or thick strands of
loose CT interspersed in between patches of
epithelial parenchyma.
 kidney -- slide 62. (Very thin stroma within
kidney proper, nice adipose CT in the hilus;
capsule is dense fibrous CT.)
 salivary gland -- slide 34. (Very thin stroma
between acini; thicker strands between
lobules.)
 pancreas -- slides 51, 52, 53. (Very thin
stroma between acini; thicker strands of loose
CT between lobules.)
 mammary gland -- slides 78, 79. (Relatively
thick stroma between epithelial tubules.)
_______________________________________
In the following hollow organs, lined by
stratified epithelium (stratified squamous or
transitional), CT lies immediately beneath the
epithelium. Try to distinguish CT from muscle.
 esophagus -- slides 57, 35, 36, 37. (Muscle
forms discrete layers.)
 tongue -- slide 32. (Skeletal muscle
comprises the bulk of the organ.)
In the digestive tract, lined by simple columnar
epithelium, the loose CT immediately beneath
the epithelium is called lamina propria. It is
relatively cellular, containing numerous
lymphocytes. Deeper is submucosa, also loose
CT but more fibrous, with fewer cells. Smooth
muscle forms a discrete layer.
 colon -- slides 47, 48, 49.
 vagina -- slide 76. (Smooth muscle is
interspersed with the CT.)
 intestine -- slides 42, 43, 44, 45, 46, 51.
 bladder -- slide 65. (Smooth muscle forms
indistinct layers beneath connective tissue.)
_______________________________________
 ureter -- slide 66. (This slide includes a good
example of adipose CT.)
 stomach -- slides 37, 38, 39, 40, 41.
Blood vessels -- slide 09.
Look for CT between the vessels.
Last updated: 23 January 2013 / dgk
Connective Tissue Practice Quizzes
To test/confirm your recognition of connective
tissue, you are encouraged to take one or more
ungraded quizzes.
□
Please indicate when you are ready for a quiz.
Your instructor will then provide a slide (see
boxes at right) on which you should locate each
of the listed structures.
Each of the listed structures should be readily
apparent in an appropriate region. Use the
eyepiece pointer at low power to indicate layers
or regions, higher power for cells and other
small details.
Do not call for a quiz until you feel confident of
your ability to recognize the listed structures.
You may also ask your instructor about any
other structure that catches your interest.
Note: As always, you are also encouraged to
seek confirmation at any time for your
recognition of structures on slides from your
reference slide set -- particularly of any features
not included in these quizzes.
□
□
□
Connective tissues of tongue:
____lamina propria / CT papillae
____fibrous CT associated with muscle
____adipocytes
____blood vessel
Connective tissues of salivary
gland:
____lamina propria
____stromal CT
____adipocytes
____blood vessel
Connective tissues of esophagus:
____lamina propria
____submucosa
____lymphoid CT
____blood vessel
Connective tissues of stomach
and duodenum:
____lamina propria
____submucosa
____lymphoid CT
____blood vessel
Last updated: 18 January 2013 / dgk
ZOOL 409
Lab Week 3
January 29, TUESDAY
__________________________________________________________________________________
Visit a working laboratory and see how
histological specimens are prepared.
Meet in Room 2055 of Life Science 3
(immediately south of LSII).
Objectives:
Appreciate the technologies and skills
involved in preparing histological
specimens:
Fixation
Embedding
Sectioning
Mounting sections on slides
Staining
*** NOTE ***
You may be given a "souvenir" slide during this
histotechniques field trip. Keep this slide use
later in the course.
Navigation from LSII: Leave LSII at southwest corner,
cross driveway southward to entrance of LS3. Elevator is
to the left of the entry. On 2nd floor, from elevator turn
left, left at cross-hallway, left again. Room 2055 is the
fourth door on the left. [For stairway instead of elevator,
take cross-hallway to left of entry, turn left (east) at end of
cross-hallway. Stairway is second door on left. Turn left
(east) at top of stairway. Room 2055 is the second door on
the left.]
This slide includes specimens from several
organs, and displays some details more clearly
than slides in our reference collections. As you
learn to recognize various organs, look for and
study them on this slide as well as on the slides
available in laboratory.
Navigation from parking lot entrance, south-west corner of
LS3: Stairway is a few steps to the right of the entrance.
Turn left (east) at top of stairway. Room 2055 is the second
door on the left.
Last updated: 20March 2013 / dgk
Today is "field trip" day for
ZOOL 409 Histology.
Meet at Rm 2055
in LS III
(the adjacent building to the south)