Supplementary Information for Biotechnology Letters
Green synthesis of gold nanoparticles by a newly isolated strain Trichosporon
montevideense for catalytic hydrogenation of nitroaromatics
Wenli Shen, Yuanyuan Qu*, Xiaofang Pei, Xuwang Zhang, Qiao Ma, Zhaojing Zhang, Shuzhen Li and Jiti
Zhou
State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental
Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian
University of Technology, Dalian 116024, China
*
Corresponding author. E-mail address: qyy@dlut.edu.cn (Y.Y. Qu)
Materials and methods
Chemicals and medium
Nitroaromatics and aromatic ammonia compounds were obtained from Sinopharm Chemical
Regent Beijing Co., Ltd. (China), including 2 -NP, 3-NP, 4-NP, o-NPA, m-NPA, 2-aminophenol,
3-aminophenol,
4-aminophenol,
o-diphenylamine
and
m-diphenylamine.
Hydrogen
tetrachloroaurate (III) hydrate (HAuCl 4 ·3H 2 O) was purchased from J&K Scientific Ltd. (China).
All other chemicals were of analytical grade or above.
The medium used in selection and cultivation was lysogeny broth medium (LB, pH 7.0), which
consisted of 10 g NaCl/l, 5 g yeast extract/l and 10 g peptone/l. Solid medium was obtained by
supplement with 2% (w/v) agar. All the media were autoclaved at 121 oC for 20 min. Tetracycline (50
mg/l) and terramycin (50 mg/l) were added to the medium before use in the process of isolation
strains.
Identification of strain WIN
Genomic DNA of strain WIN was applied to amplify the 26S rRNA gene by polymerase chain
reaction (PCR), and PCR products were sequenced by TaKaRa Biotechnology Co. Ltd. (Dalian,
China). The BLAST program was taken to compare the sequence with those in GenBank, and the
related sequences were aligned using Clustal X (1.8). The aligned sequences were used to
construct phylogenetic tree using Neighbor-joining method by MEGA (Version 5.1) with 1000
bootstrap replicate.
Results and discussion
Isolation and identification of strain WIN
Strain WIN was spheroidicity as shown in SEM image ( Supplementary Figure 1a), and generated
round colonies with centric points on agar plates (Supplementary Figure 1b). Based on the
analysis of 26S rRNA gene sequence, strain WIN exhibited 99% homology to Trichosporon
montevideense (Genebank accession number JQ965893), thus it was identified as Trichosporon
montevideense. The phylogenic tree demonstrating the relationship between strain WIN and
other strains was shown in Supplementary Fig. 1c. The partial 26S rRNA gene sequence (550 bp)
of strain WIN was deposited in GenBank database under the accession number KP676895. Str ain
WIN has been deposited in the China General Microbiological Culture Collection Center
(CGMCC) under accession number CGMCC 10368.
Supplementary Figure
Supplementary Figure. 1 The SEM image (a) and agar plate photo (b) of strain WIN. (c) Phylogenetic
tree of Trichosporon montevideense WIN and related species