Barz CS, Bessaih T, Abel T, Feldmeyer D, Contreras D
Altered resonance properties of somatosensory responses in mice deficient for the
schizophrenia risk gene Neuregulin 1
Supplementary Materials
a
3 Hz
8 Hz
10 Hz
15 Hz
b
MFR [Hz]
c
20
STIM
BL
15
10
5
0
1
3
5
8 10 12 15
stimulation frequency [Hz]
Fig. S1 Frequency-dependent modulation of spiking activity in C57BL/6 mice. a
Stimuli were presented at 7 frequencies ranging from 1-15 Hz. Shown are four
sample stimuli at 3 Hz, 8 Hz, 10 Hz and 15 Hz. b PSTH of three example MUAs (top
to bottom; bin size: 1 ms). Mean firing rates were calculated across 40 trials for each
stimulation frequency. c Average mean firing rates (n = 14 MUAs) during stimulation
(black circles) and baseline conditions (grey circles)
15
power SNR
12
9
6
3
0
1
3
5
8
10
12
stimulation frequency [Hz]
15
Fig. S2 Signal-to-noise ratio of absolute power during stimulation conditions in
C57BL/6 mice. Fourteen C57BL/6 mice LFPs were analyzed for changes in absolute
power at the stimulation frequency relative to absolute power in the same frequency
range measured during baseline conditions
Fig. S3 Spike-field coherence in C57BL/6 mice. a Examples of time-frequency plots
of coherence during 3 Hz (left) and 8 Hz (right) stimulation conditions (stimulus onset
at time 0). Coherence is color-coded from 0 to 0.9 (see color bar on the right). b
Average coherence spectra of C57BL/6 responses (n = 14 spike-LFP pairs) during
stimulation (0.05-3 s after stimulus onset) and baseline conditions (-3 to -0.05 s
before stimulus onset). c Coherence during stimulation conditions at the
corresponding stimulation frequency. d Coherence during baseline conditions.
Baseline coherence values did not differ across conditions (One-way repeated
measures ANOVA, p > 0.05) and were therefore collapsed. e Difference in
coherence during stimulation conditions relative to average baseline values
a
2.5
2
8-15 Hz adaptation
2.5
1 Hz
3 Hz
5 Hz
adaptation ratio
adaptation ratio
b
1-5 Hz adaptation
1.5
1
0.5
0
8 Hz
10 Hz
12 Hz
15 Hz
2
1.5
1
0.5
0
0
1
2
3
0
time [s]
1
2
3
time [s]
Fig. S4 MUA response adaptation in C57BL/6 mice. Adaptation ratios were
calculated by dividing each response to successive stimuli in a stimulus train by the
response to the first stimulus. a Population average of responses to 1-5 Hz
stimulation (n = 14 MUAs). Inset: Example PSTH (3 Hz). Indicated below are the
time windows (50 ms each) for measuring responses to the first stimulus (red bar)
and responses to successive stimuli in the stimulus train (blue bars). b Population
average of responses to 8-12 Hz stimulation (n = 14 MUAs)
mean firing rate
MFR [Hz]
40
0-1 s
2-3 s
30
20
10
0
b
adaptation
120
adaptation ratio
a
90
60
30
0
1
3
5
8 10 12 15
stimulation frequency [Hz]
1
3
5
8 10 12 15
stimulation frequency [Hz]
Fig. S5 Temporal dynamics of MUA response adaptation in C57BL/6 mice. a Mean
firing rates of MUAs (n = 14) during an early (0-1 s; black diamonds) and late time
window (2-3 s; gray diamonds; see inset for enlarged scale). b Adaptation ratio
calculated by dividing the sustained response during the early time window (0-1 s) by
the response during the late time window (2-3 s). The largest ratio and thus the
largest response adaptation is seen around 8 Hz stimulation
a
8 Hz
10 Hz
15 Hz
spike count
3 Hz
phase [degrees]
phase locking
vector strength r
1
0.8
0.6
*
0.4
0.2
0.05-1 s
2-3 s
0
1
3
5
8 10 12 15
stimulation frequency [Hz]
c
temporal dispersion d [s]
b
temporal dispersion
0.16
0.05-1 s
2-3 s
0.12
0.08
*
0.04
0
1
3
5
8 10 12 15
stimulation frequency [Hz]
Fig. S6 Temporal fidelity of neuronal responses in C57BL/6 mice. a Phase
histograms of three MUAs at four sample stimulation frequencies (3 Hz, 8 Hz, 10 Hz,
15 Hz). The three example MUAs (top to bottom) are the same as depicted in Fig.
S1b. Phase histograms were calculated for sustained responses 0.05-1 s following
stimulus onset. Values of vector strength r and temporal dispersion d [ms] are
indicated for each unit and condition. b Average vector strength 0.05-1 s (black
triangles; n = 14) and 2-3 s post stimulus onset (gray triangles; n = 12). * Significant
differences in vector strength between the first and second time window (see Table
S1a for details). c Temporal dispersion 0.05-1 s (black triangles; n = 14) and 2-3 s
(gray triangles; n = 12) following stimulus onset. * Significant differences in temporal
dispersion between the first and second time window (see Table S1b for details)
Table S1 Extended statistical information on comparisons in C57BL/6 mice. Same
conventions as in Table 1 (see main text). Sample sizes (MUAs) are indicated for
quantifications during the first time window (0.05-1 s; n1) and the second window (23 s, n2). P-values below the Bonferroni-corrected significance level (0.007) are
highlighted in bold
row
Fig.
a
S6c
b
S6d
1 Hz
3 Hz
5 Hz
8 Hz
10 Hz
12 Hz
15 Hz
measure
stats
vector
U
49
60
99
98
96
96
71
strength
p
0.076
0.373
0.096
< 0.0001
< 0.0001
0.005
0.330
n1
14
14
14
14
14
14
14
n2
12
11
10
7
7
8
8
temporal
t (df)
1.9 (24)
1.4 (23)
-1.1 (22)
-9.2 (19)
-6.1 (19)
-3.2 (20)
-1.0 (20)
dispersion
p
0.063
0.165
0.275
< 0.0001
< 0.0001
0.005
0.341
n1
14
14
14
14
14
14
14
n2
12
11
10
7
7
8
8