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SUPPORTING INFORMATION FOR
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Cadmium accumulation kinetics in Rhodococcus jostii RHA1 and
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potential effects of brominated flame retardants
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Shen Xu, Zhong-Bo Wei, Lian-Hong Wang, Rong Ji*, Liu-Yan Yang, Ai-Jun Miao*
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State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment,
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Nanjing University, Nanjing, Jiangsu Province 210023, China
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8 pages, 2 Tables, and 5 Figures
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*Corresponding authors:
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R. Ji, School of the Environment, Nanjing University, Mail box 24, Xianlin Road 163,
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Nanjing 210046, Jiangsu Province, China PRC (mailing address), 86-25-89680581 (phone),
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86-25-89680569 (fax), ji@nju.edu.cn (email)
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A. J. Miao, School of the Environment, Nanjing University, Mail box 24, Xianlin Road 163,
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Nanjing 210046, Jiangsu Province, China PRC (mailing address), 86-25-89680255 (phone),
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86-25-89680569 (fax), miaoaj@nju.edu.cn (email)
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Page S1
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Table S1. Chemical components and their concentrations in mineral medium (MM).
Components
Concentration (M)
Components
Concentration (M)
NaNO3
1.2×10-2
C6H12O6
2×10-2
K2SO4
9.8×10-4
ZnSO4·7H2O
2×10-6
CaSO4
5×10-5
MnSO4·7H2O
2×10-6
MgSO4·7H2O
1.5×10-4
CuSO4·5H2O
2×10-6
KH2PO4
3.9×10-3
CoSO4·7H2O
4×10-6
K2HPO4·3H2O
6.1×10-3
NaMoO4·H2O
4×10-6
H3BO3
2×10-6
FeSO4·7H2O
8×10-5
C12H10
1×10-2
Page S2
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Table S2. Total dissolved ([Cd]T, M) and free Cd ion ([Cd2+]F, M) concentration in Treatments A-H of the short-term experiment of Cd uptake at
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different ambient levels as well as in the short-term experiment of Cd uptake without (control) and with the presence of 1 μM EDTA, 1 μM NTA,
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or 100 μM citrate.
Cd uptake at different ambient levels
Cd uptake without and with the presence of EDTA, NTA, or citrate
Treatments
[Cd]T
[Cd2+]F
[Cd]T
[Cd2+]F
[Cd]T
[Cd2+]F
A
8.90×10-10
8.20×10-10
1.09×10-9
1.00×10-9
1.75×10-8
1.00×10-9
B
8.90×10-9
8.20×10-9
1.09×10-8
1.00×10-8
1.54×10-7
1.00×10-8
-8
-8
-7
1.00×10
-7
C
2.70×10
2.50×10
1.09×10
7.34×10-7
1.00×10-7
D
8.90×10-8
8.20×10-8
1.09×10-6
1.00×10-6
2.03×10-6
1.00×10-6
E
2.70×10-7
2.50×10-7
9.87×10-7
1.00×10-9
1.73×10-9
1.00×10-9
F
8.90×10-7
8.20×10-7
1.10×10-6
1.00×10-8
1.73×10-8
1.00×10-8
G
2.70×10-6
2.50×10-6
1.11×10-6
1.00×10-7
1.73×10-7
1.00×10-7
H
8.90×10-6
5.60×10-6
2.09×10-6
1.00×10-6
1.72×10-6
1.00×10-6
Ligands
control
EDTA
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Page S3
Ligands
NTA
citrate
OD600
3.0
1.5
LB
MM
0.0
0
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20
40
60
80
100
Time (h)
Fig. S1. The growth curve of Rhodococcus jostii RHA1 in LB and mineral medium (MM).
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Page S4
EC50 = 14.4 nM
TBBPA
BDE-3
0.10
-1
1.0
b
 (h )
Relative change of 
a
0.05
0.5
0.0
0.00
0.1
1
10
0
100
300 1000
Concentration of
TBBPA or BDE-3 (g/L)
100 1000
2+
[Cd ]F (n)
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Fig. S2. (a) Relative change of the cell-specific growth rate () of Rhodococcus jostii RHA1
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with different concentrations of free Cd ion ([Cd2+]F); (b)  of Rhodococcus jostii RHA1 with
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the addition of 0, 100, 300, and 1000 μg/L TBBPA or BDE-3. Data are mean ± standard
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deviation (n = 2).
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Page S5
0.04
[Cd]intra（ fg/cell）
0.02
0.00
2.0
1.0
A
B
C
D
a
E
F
G
H
b
0.0
0
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40
80
120
160
Exposure time (min)
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Fig. S3. Increase of [Cd]intra with exposure time in treatments A-H of the short-term
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experiment of Cd uptake at different ambient levels. Cd concentrations of treatments A-H are
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listed in SI, Table S2. Data are mean ± standard deviation (n = 2).
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Page S6
0.02
control
EDTA
NTA
citrate
[Cd]intra(fg/cell)
0.01
0.2
0.1
0.00
a 0.0
0.04
1.0
0.02
0.5
0.00
b 0.0
300
0
0
100
200
c
d
50
100
150
200
Exposure time (min)
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Fig. S4. Increase of [Cd]intra with exposure time in the short-term experiment of Cd uptake
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without (control) and with the addition of 1 μM EDTA, 1 μM NTA, or 100 μM citrate with
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[Cd2+]F of (a) 1, (b) 10, (c) 100, and (d) 1000 nM, respectively. Data are mean ± standard
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deviation (n = 2).
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Page S7
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[Cd2+]F (nM)
180
TBBPA
BDE
90
0
0
30 100 300 1000
TBBPA or BDE concentration (g/L)
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Fig. S5. Free cadmium concentration ([Cd2+]F, nM) measured by voltammetry in the presence
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of 0, 30, 100, 300, and 1000 g/L TBBPA or BDE-3. Data are mean ± standard deviation (n =
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2).
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Page S8