Agaroses Flasks
handling instructions
Un-tighten the cap without removing it from the flask.
Microwave for one minute at maximum power. Agitate the flask gently and introduce it again in the microwave for another
30 seconds at low power, and gently agitate again.
Repeat this until it dissolves completely: Agarose has to appear totally transparent and without bubbles.
Let the solution to cool during 1 or 2 minutes.
Once the agarose is perfectly dissolved, with no bubbles, pour the necessary volume of the solution in the gel tray.
Let it set at room temperature until the solution forms a hard gel.
Keep flask totally closed in refrigerator until next use.
For a total gelification process, it is recommended, to keep MS-8 and LM SIEVE gels at 4ºC to 8ºC for one hour.
Warning! Always use safety mask, gloves, and glasses. Open the flask gently to prevent burns.
Analytical
separation
≥1000 bp
Analytical
separation
≤1000 bp
Preparative
electrophoresis
PFGE
DNA typing
Blotting
Fine resolution
In-gel
applications
Protein
electrophoresis
D1 LE
LM Sieve
MS8
TIPS FOR GEL PREPARATION

Adjust time and power settings according to your
microwave output strength.

Low melt agarose gels need to sit for an additional 30
min or overnight at 4-8°C to allow a total gelling process.

Overboiling can cause agarose hydrolysis and lower gel
strength.


For total agarose melting: boil the solution only enough to
affect total dissolution.
Low melting or low percentage gels: it is important to run
electrophoresis in a cold buffer. High voltages can cause
overheating of the buffer which can melt the gel.

Buffer composition can be determinative in the gelling
process: if agents that disrupt hydrogen bond formation
are added to

The buffer, melting temperature and gel strength will
decrease, or even inhibit gel formation.

Once the gel is set, flood with the buffer. The gel can be
stored refrigerated for several days.

To avoid bubble formation: cool to 60°C and pour carefully
into the gel cassette.

After pouring, allow the gel to cool gradually; rapid cooling
will cause an irregular gel matrix and band distortion
during electrophoresis.
9008
AGAROSE D1 , 1% TAE, Non toxic Stain
12 x 100 ml Flask
9009
AGAROSE D1 , 1% TAE, Non toxic Stain
10 x 200 ml Flask
9015
AGAROSE D1 , 2% TAE, Non toxic Stain
12 x 100 ml Flask
9017
AGAROSE D1 , 2% TAE, Non toxic Stain
10 x 200 ml Flask
9034
AGAROSE D1 , 3% TAE, Non toxic Stain
12 x 100 ml Flask
9035
AGAROSE D1 , 3% TAE, Non toxic Stain
10 x 200 ml Flask
9013
AGAROSE MS 8, 3% TAE, Non Toxic Stain
12 x 100 ml Flask
9036
AGAROSE MS 8, 3% TAE, Non Toxic Stain
10 x 200 ml Flask
9037
AGAROSE LM Sieve, 4 % TAE Non Toxic Stain
12 x 100 ml Flask
9038
AGAROSE LM Sieve, 4 % TAE Non Toxic Stain
10 x 200 ml Flask
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D-1 AGAROSE
More reliable and easier handling: extraordinary mechanical resistance.
Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
Excellent gel transparency and visibility.
Absence of toxicity (unlike polyacrylamide, which is toxic).
MS-8 AGAROSE
An agarose for molecular screening that improves resolution of small DNA fragments and PCR products. CONDA has
produced MS-8 Agarose for applications that require efficient separation of small DNA fragments and PCR products.
High resolution of short PCR and DNA fragments.
Improved clarity of the gel, enhancing visibility.
Better handling than similar products: stronger gel structure and higher gel strength. Chances of gels breaking or cracking
when handled are greatly minimized, even at low agarose concentrations.
LM SIEVE
LM SIEVE Agarose is a low melting temperature agarose with the highest resolving capacity for DNA fragments smaller than
1000 bp, especially PCR products ranging from 200 to 800 bp. This agarose is GQT (Genetic Quality Tested) certified. This
ensures that In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. LM SIEVE
Agarose can be used at high concentrations, forming gels with excellent clarity and a higher sieving capacity than standard
melting agaroses.
Easy recovery of small DNA fragments suitable for cloning or enzymatic processing as it is ideal for digestion by agarose
enzymes.
High resolving capacity for DNA fragments ≤ 1000 bp.
WHY DO WE USE NON TOXIC STAIN?
Pronasafe Nucleic Acid Staining is a safe nucleic acid stain, alternative to the traditional ethidium bromide (EtBr) stain
for detecting nucleic acid in agarose gels. It allows visualizing DNA and RNA, as they are separated during agarose gel
electrophoresis and isolating DNA fragments for subcloning without introducing mutations. This stain emits fluorescence
when bound to DNA or RNDA. It has two wavelengths for excitation, one centered at 309 nm and another at 419 nm. In
addition, it has one visible excitation at 514 nm. The fluoresce emission is centered at 537 nm.
FEATURES
99 NON toxic, NON mutagenic, NON carcinogenic.
99 More resolution than EtBr.
99 No hazard waste.
99 Used for DNA and RNA detection.
99 Isolation of DNA fragments for subcloning without
introducing mutations, normally caused by EtBr.
NOTE: Repeated melting of gels containing non toxic stain may result in low sensitivity. An overheating of the agarose with non toxic stain
could cause a loose of 5% of sensitivity.