sLB AGAR
CAT Nº:1432
Medium designed to increase bacterial growth and leads to high yields of low
copy plasmids and extra high yields of high copy plasmids.
Special Peptone Mixture
Salts
Yeast Extract
Bacteriological Agar
Final pH 7.0 ± 0.2 at 25ºC
PREPARATION
Suspend 55 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil
for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC, mix well and
dispense into plates. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
sLB AGAR is a new medium to complete the sLB product line and is the ideal medium to be used along with sLB Broths
(Cat. 1163, Cat. 1199) , which have been especially designed to give high yields of low copy plasmids and extremely
high yields of high copy plasmids, or with the rest of molecular biology brothers. A higher number of colonies are
obtained, so a better recuperation of plasmid can be achieved.
The special peptone mixture, yeast extract, agar and salts supply essential growth factors such as nitrogen, carbon,
sulfurs, minerals and vitamins, particularly the B group. Sodium chloride supplies essential electrolytes: Sodium ions for
transport and osmotic balance. Bacteriological agar is the solidifying agent.
CELL GROWING TEST
To make this test it must be prepared a cell culture by inoculating a 15 ml tube of sLB Broth with E.coli sample. This
tube is incubated at 37ºC for 24 hours without aeration. A 10 ul of an aliquot of a 104 cells/ml was distributed in the sLB
agar plates using a glass “hockey stick” spreader. The inoculated plates were incubated at 37ºC overnight.
After the incubation the colonies were counted and two colonies were measured its diameter.
Microorganisms
Average Colony Diameter in mm
Escherichia coli ATCC C600
Plate 1
Plate 2
Range among
1.70
410
3.80
480
1.0 - 4.0 mm
400 - 500 mm
BIBLIOGRAPHY
Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl (ed.). 1994. Current protocols in molecular biology, vol. 1. Greene
Publishing Associates, Inc., Brooklyn, N.Y.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
1
LABORATORIOS CONDA, S.A.
www.condalab.com