Table 1. Molecular markers (including antigens and transcription factors) for
identifying differentiated cell types as well as neural stem/ progenitor cells.
Antigen/Transcription Factor
Neuronal Markers
Beta tubulin III (TuJ1)
MAP2
Description
TOAD64
Presenilin
Doublecortin
Neuron specific-enolase
SCG10 (superior cervical ganglion)
Hu
GAP43 (growth associated protein)
immature and mature neurons
a: later than p14, b: mature, c: immature to
mature
mature neurons
L: immature neuron, M and H: postmitotic
neuron
newly postmitotic neurons
postmitotic neuron and other cells
migrating neuron
relatively immature and postmitotic neuron
mature neuron, related to regeneration
neuron-specific RNA binding protein
growth cone, related to growth and regeneration
Transcription Factors
Neurogenin1/2
NeuroD
Mash1
Math2
Tbr1
Hoxa2,b2
Phox2b
Nkx2.2
Pax6/7
FGF8
Wnt1/Lmx1b
Dlx5/6
cortical neuroblast
pyramidal neuron
neuroblast to Interneuron
neuroblast
postmitotic neurons in cerebral cortex
rostral hindbrain
central and peripheral autonomic nervous system
neuroblast to interneuron
neuroblast to pyramidal neuron
isthmus
isthmus
GABAergic interneuron
NeuN
Neurofilament
Synapse-related
Synaptophysin
Synaptotagmin1
Synapsin1
Neurabin
SNAP(synaptosomal associated
protein) 25
Synaptobrevin
Syntaxin
membrane of synaptic vesicle, endocrine cell
presynaptic vesicle
membrane of synaptic vesicle
synapse in postmitotic neuron
plasmamembrane
synaptic vesicle
plasmamembrane
Table 1. (cont.) Molecular markers (including antigens and transcription factors) for
identifying differentiated cell types as well as neural stem/ progenitor cells.
Antigen/Transcription Factor
Description
Astrocyte Markers
GFAP
S100b
Vimentin
Nestin
astrocyte, radial glia, stem/progenitor cell
astrocyte, ependymal cell, some neuron
glial progenitor, radial glia, immature astrocyte
immature astrocyte, stem cell, muscle
Radial Glial Markers
BLBP (brain-lipid binding protein)
Vimentin
GFAP
GLAST
RC1/2
Nestin
REST (RE1-silencing transcription
factor)
Oligodendrocyte Markers
PLP/DM20
CNPase
Myelin-associated/
oligodendrocytic basic protein
(MOBP)
GalC (galactocerebroside)
Myelin basic protein (MBP)
Myelin associated glycoprotein
(MAG)
Claudin11 / OSP
NOGO (neurite outgrowth inhibitor)
Transcription factors
Olig1/2
radial glia, multipotent progenitor
glial progenitor, radial glia, immature astrocyte
radial glia, astrocyte, stem/progenitor cell
radial glia to postmitotic astrocyte
radial glia
immature astrocyte, stem cell, muscle
nonneuronal progenitor
perinuclear and processes of mature
oligodendrocyte (myelin)
membrane of CNS myelin
oligodendrocyte (myelin)
mature oligodendrocyte, not in O2A
processes of mature oligodendrocyte (myelin),
bone marrow, immune system
oligodendrocyte (myelin)
immature to mature oligodendrocyte
oligodendrocytes, endoplasmic reticulum of CNS
myelin, not in Schwann cell
oligodendrocyte lineage, some subtypes of neuron
Table 1. (cont.) Molecular markers (including antigens and transcription factors) for
identifying differentiated cell types as well as neural stem/ progenitor cells.
Antigen/Transcription Factor
Description
Other cell types
Ependymal/choroid plexus
TTR
Noggin
S100b
epithelial cell of choroid plexus
ependymal and early neuron
astrocyte, ependymal cell, some neurons
Smooth muscle
SMA
Microglia
lectin
CD68
CD11c
Multipotent Stem/Progenitor Markers
Nestin
immature astrocyte, all dividing neural stem cell,
muscle
Msi1
immature progenitor, neural stem cell, some
astrocyte
GFAP
radial glia, astrocyte, stem/progenitor cell
Notch1
immature cell, SVZ in adult brain, many tissues in
embryo
gp130 (granulocyte glycoprotein)
many tissues in embryo
Nucleostemin
dividing stem cell
Sox1/2
neuroepithelium, neural stem cell, less in
progenitor
Telomerase activity/TERT
embryonic, hematopoietic, and neural stem cell,
some in progenitor
ABCG2 (BCRP1)
hematopoietic stem cell, myogenic progenitor,
nestin positive pancreastic cell
EGFR
widely in embryo
FGFR
widely in embryo
Lex1
embryonic and adult neural stem cell, some
differentiated cell type
CD133/CD34
hematopoietic and neural stem cell
Table 1. (cont.) Molecular markers (including antigens and transcription factors) for
identifying differentiated cell types as well as neural stem/ progenitor cells.
Antigen/Transcription Factor
Commited Progenitor Markers
A2B5
O4
PSA-NCAM
Beta tubulin III (TuJ1)
Description
oligodendrocyte progenitor, glial progenitor, early
oligodendrocyte (not mature)
immature oligodendrocyte progenitor,
oligodendrocyte, Schwann cell
cortical progenitor, ependymal cell, glial restricted
progenitor, neuronal progenitors
immature neuroblast, mature neuron