Figure 6:
(a)
(b)
Figure 6. Demonstration of the capability of two-photon 3-D image cytometry in rare event
detection. Mouse embryonic stem cells expressing cyan and yellow fluorescent
proteins were mixed at a ratio of 1:10 down to 1: 105. Mouse embryonic stem cells
spontaneously formed 3-D clusters in cell culture. (a) The top panels were collected
in the yellow channel and the bottom panels were collected in the cyan channel. The
left and right sets of images are at different depths of an embryonic stem cell cluster.
Cyan cells are seen in both channels, whereas yellow cells are only seen in the
yellow channel. (b) A plot of the experimentally measured mixing ratio versus the
expected mixing ratio demonstrates that dilution down to 1 in 105 can be accurately
measured.
1