vii TABLE OF CONTENTS CHAPTER 1 2 TITLE PAGE DECLARATION ii DEDICATIONS iii ACKNOWLEDEGMENTS iv ABSTRACT v ABSTRAK vi TABLE OF CONTENTS vii LIST OF TABLES x LIST OF FIGURES xii LIST OF ABBREVIATIONS xvii LIST OF APPENDICES xix INTRODUCTION 1 1.1 Background 1 1.2 Problem Statement 5 1.3 Goal 7 1.4 Objectives 7 1.5 Scope of the Study 8 1.6 Significance of the Study 11 LITERATURE REVIEW 13 2.1 Introduction 13 2.2 Water Quality 17 2.3 River Morphology/Hydrology 19 2.4 Bio-Assessment and Bio-Indicators 21 2.5 Calculation of Indices 25 viii 2.5.1 Diversity Index 25 2.5.2 Evenness Index 25 2.5.3 Richness Index 26 2.5.4 Index of Dominance 26 2.5.5 Ephermeroptera, Plecoptera, and Trichoptera Index (EPT Index) 3 2.6 Habitat Characteristics 27 2.7 Urbanization 30 2.8 Logging 32 2.9 Agriculture 34 METHODOLOGY 36 3.1 Research Design and Procedure 36 3.1.1 Sampling Site 36 3.1.2 Sampling Station 37 3.1.3 Sampling Procedure 43 3.2 Operational Framework 46 3.3 Data Sources 47 3.4 Instrumentation and Data Analyses 47 3.4.1 Water Quality 47 3.4.2 Hydrology 50 3.4.3 Macro-invertebrate Sampling and Analysis 52 3.4.4 Habitat Assessment 57 3.4.5 Correlation and Relationship Analysis 3.5 4 27 Integration of the River Ecosystem Attributes RESULTS 4.1 4.2 63 65 66 Physico-Chemical Properties For Water Quality 66 River Morphology/Hydrology 78 4.2.1 River Discharge 79 4.2.2 Riverbed Deformation 81 ix 4.3 4.4 Habitat Assessment 85 4.3.1 Sediment Sizes Distribution 86 4.3.2 River Riparian Cover and Compositions 96 4.3.3 Canopy Cover 97 4.3.4 River Meanders 101 4.3.5 Large Woody Debris 101 Benthic Macro-Invertebrates 4.4.1 Benthic Macroinvertebrate Compositions 103 4.4.2 Biological Diversity Index 108 4.4.3 Ephemeroptera, Plecoptera, Trichoptera (EPT) Index 4.5 102 115 Correlation Between Habitat Characteristics, Physicochemical Water Quality and Biological Index 117 5 DISCUSSION 123 6 CONCLUSION AND RECOMMENDATION 136 6.1 Conclusion 136 6.2 Recommendations 137 REFERENCES Appendices A - K 142 164- 340 x LIST OF TABLES TABLE NO. TITLE PAGE 1.1 Flow for the Proposed Project 10 3.1 Sampling Site Descriptions 37 3.2 Vegetative Coding System Used in Categorizing Riparian Vegetation 63 3.3 Coding for Categorical Data 64 4.1 Rainfall Data for Sungai Mengkibol, Madek, Dengar and Hulu Dengar from 2008 to 2010 4.2 80 River Discharges between November 2008 to June 2010 for all the rivers 81 4.3 River Substrate Median Sizes (D50) 86 4.4 Benthic Macro-invertebrate Composition for Different Land Uses 105 4.5 EPT Indices from November 2008 to June 2010 117 4.6 Correlation between Physicochemical Water Quality and Biological Index 4.7 Correlation between Physicochemical Water Quality and Physical Characteristics 4.8 120 Correlation between Large Woody Debris (LWD) and Biological Index 4.11 119 Correlation between Substrate Composition (D50) and Biological Index 4.10 119 Correlation between Canopy Cover and Biological Index 4.9 118 121 Correlation between Riparian Composition and Biological Index 121 xi 4.12 Correlation between River Discharge and Biological Index 6.1 Recommended River Health Standard for Malaysian Rivers 6.2 139 Recommended Biodiversity Index for River Health Assessment 6.3 122 140 Recommended Habitat Characteristics for River Health Assessment 140 xii LIST OF FIGURES FIGURE NO. TITLE PAGE 1.1 Study Area and Sampling Station 9 3.1 Sampling Stations at Sungai Mengkibol 39 3.2 Sampling Stations at Sungai Madek 40 3.3 ampling Stations at Sungai Dengar 41 3.4 Sampling Stations at Sungai Hulu Dengar 42 3.5 Sampling Stations at Sungai Gunung Berlumut 43 3.6 Research Flowchart 46 3.7a Channel Cross Sectional Diagram 51 3.7b Conducting River Flow Measurement 52 3.8 Surber Net 53 3.9 Sampling of Benthic Macro-invertebrate Using Surber Net 54 3.10 Pool and Riffle Diagram 59 3.11 Measuring Pebble Count 59 3.12 Schematic Diagram of Assessing Particle Size During Pebble Count 60 3.13 Schematic Diagram of Assessing Canopy Cover 62 4.1 issolved Oxygen (DO) Concentrations at All the Sampling Stations and Events 67 4.2 pH Readings at All the Sampling Stations and Events 68 4.3 Turbidity Concentrations at All the Sampling Stations and Events 69 4.4 Salinity at All the Sampling Stations and Events 70 4.5 Temperature Readings at All the Sampling Stations and Events 70 xiii 4.6 Conductivity at All the Sampling Stations and Events 4.7a Biochemical Oxygen Demand (BOD) at All the Sampling Stations and Events 4.7b 73 Ammoniacal Nitrogen (NH3-N) at All the Sampling Stations and Events 4.7d 72 Chemical Oxygen Demand (COD) at All the Sampling Stations and Events 4.7c 71 74 Suspended Solids (SS) at All the Sampling Stations and Events 75 4.8a Nitrate at All the Sampling Stations and Events 76 4.8b Phosphate at All the Sampling Stations and Events 77 4.9 Water Quality Index (WQI) at All the Sampling Stations and Events 78 4.10 Riverbed changes of Sungai Mengkibol 78 4.11 Riverbed changes of Sungai Madek 83 4.12 Riverbed changes of Sungai Dengar 84 4.13 Riverbed changes of Sungai Hulu Dengar 85 4.14 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 07 November 2008 4.15 88 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 20 March 2009 4.16 88 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 16 June 2009 4.17 89 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 08 August 2009 4.18 89 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 27 March 2010 89 xiv 4.19 Sediment Size Distribution for Sungai Mengkibol Upper Reach (Left) and Lower Reach (Right), Sampling on 19 June 2010 4.20 90 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 09 November 2008 4.21 90 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 22 March 2009 4.22 90 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 18 June 2009 4.23 91 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 09 August 2009 4.24 91 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 28 March 2010 4.25 91 Sediment Size Distribution for Sungai Madek Upper Reach (Left) and Lower Reach (Right), Sampling on 20 June 2010 4.26 92 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 08 November 2008 4.27 92 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 21 March 2009 4.28 92 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 17 June 2009 4.29 93 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 10 August 2009 93 xv 4.30 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 29 March 2010 4.31 93 Sediment Size Distribution for Sungai Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 21 June 2010 4.32 94 Sediment Size Distribution for Sungai Hulu Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 17 June 2009 4.33 94 Sediment Size Distribution for Sungai Hulu Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 10 August 2009 4.34 95 Sediment Size Distribution for Sungai Hulu Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 29 March 2010 4.35 95 Sediment Size Distribution for Sungai Hulu Dengar Upper Reach (Left) and Lower Reach (Right), Sampling on 21 June 2010 96 4.36 Biodiversity Index in November 2008 98 4.37 Biodiversity Index in March 2009 98 4.38 Biodiversity Index in June 2009 99 4.39 Biodiversity Index in August 2009 99 4.40 Biodiversity Index in March 2010 100 4.41 Biodiversity Index in June 2010 100 4.42 The Density of LWD in all the Stations at Every Sampling Event 102 4.43 Biodiversity Index in November 2008 112 4.44 Biodiversity Index in March 2009 113 4.45 Biodiversity Index in June 2009 113 4.46 Biodiversity Index in August 2009 114 4.47 Biodiversity Index in March 2010 114 4.48 Biodiversity Index in June 2010 115 xvi 5.1 Interaction between physicochemical water quality with riparian composition, canopy cover, LWD and channel deformation 5.2 129 Interaction between benthic macroinvertebrate with substrate compositions, riparian composition, physicochemical water quality, river discharge, LWD and canopy cover 5.3 131 The Interplay of Various Ecological Factors on Aquatic Life Forms – An Important Determinant for a Healthy River 6.1 133 Recommended River Classification Based on River Health 138 xvii LIST OF ABBREVIATIONS WQI - Water Quality Index INWQS - Interim National Water Quality Standards for Malaysia EPT - Ephemeroptera, Plecoptera and Trichoptera LWD - Large Woody Debris DOE - Department of Environment NTU - Nephelometric Turbidity Unit NPK - Nitrogen, Phosphorus and Potassium USEPA - United States Environmental Protection Agency PNMAP - Pacific Northwest Aquatic Monitoring Partnership BOD - Biochemical Oxygen Demand COD - Chemical Oxygen Demand DO - Dissolved Oxygen NH3-N - Ammoniacal Nitrogen SS - Suspended Solids CBOD - Carbonaceous Biochemical Oxygen Demand K2Cr2O7 - Potassium Dichromate H2SO4 - Sulfuric Acid TS - Total Solids TDS - Total Dissolved Solids + H - Hydrogen ions H3O+ - Hydronium ions OH- - Hydroxide ions Fe - Ferum Al - Aluminium Mn - Manganese NO3-N - Nitrate Nitrogen NH4-N - Ammonium Nitrogen xviii PO4-P - Orthophosphate AN - Ammoniacal Nitrogen YSI - Yellow Springs Instrumentations APHA - Standard Methods for the Examination of Water and Wastewater CREAS - Christchurch River Environment Assessment Survey xix LIST OF APPENDICES APPENDIX TITLE PAGE A Regulation and Guidelines 164 B River Gauging Data Sheet 167 C Wolman’s Pebble Count 193 D Summary of Biodiversity Index 259 E Graft of Biodiversity Index 263 F Comparison between Biological Index, D50, Water Quality Index (WQI) and River Classes 267 G Taxonomy 273 H River Physical Characteristics 279 I Results of One-way ANOVA and Chi-square Test 283 J Rainfall Data 326 K Field Study Form 340