SLR-KP – 67
*SLRKP67*
Seat
No.
M.Sc. Biotechnology (Part – I) (Semester – I) Examination, 2015
Paper No. – IV : BIOSTATISTICS AND BIOINFORMATICS
Day and Date : Wednesday, 22-4-2015
Total Marks : 70
Time : 11.00 a.m. to 2.00 p.m.
Instructions : 1)
2)
3)
4)
Part – I, question 1 is compulsory.
Attempt any four questions from Part – II.
Figures to the right indicate full marks.
Answers to the Part – I and Part – II are to be written in
same answer booklet only.
PART – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives :
7
1) _______ is one of the protein information resource.
a) NCBI
c) DDBJ
b) PIR
d) EBI
2) The primary database of protein is
a) Swiss prot
b) Trembl
c) Both a) and b)
d) None
3) BLAST is ________ sequence alignment tool.
a) multiple
b) pairwise
c) both a) and b)
d) none
4) _________ is one of the homology modeling method.
a) parsimony
c) neighbor joining
b) likelihood
d) all
P.T.O.
SLR-KP – 67
*SLRKP67*
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5) A set of all possible data values for a subject under consideration is called
a) descriptive statistics
b) a sample
c) a population
d) statistics
6) The number of occurrences of a data value is called
a) the class limits
b) the frequency
c) the cumulative frequency
d) Ogive
7) An organization of observed data into tabular form in which classes and
frequencies are used is called
a) the bar chart
b) the pie chart
c) the histogram
d) frequency polygon
B) Definitions :
7
1) Proteome
2) MSA
3) Global alignment
4) Constant
5) Mean
6) Histogram
7) Population.
PART – II
Answer any four of the following :
2. Write a note on nucleotide sequence databases.
14
3. Add a note on methods of sequence alignment.
14
4. Define table. Explain the components of table.
14
5. Write a detail account on measures of central tendency.
14
*SLRKP67*
SLR-KP – 67
-3-
6. Answer any two :
14
1) Write a note on composite protein databases.
2) Add a note on methods of protein secondary structure prediction.
3) Obtain median for the following data.
M
N
a
o
.
r
k
o
s
f
s
t
u
d
e
n
t
s
$
'
"
#
$
#
&
%
7. Answer any two :
"
&
14
1) Applications of phylogenetics in bioinformatics.
2) Advantages and disadvantages of standard deviation.
3) Types of distribution in probability.
_______________
SLR-KP – 68
*SLRKP68*
Seat
No.
M.Sc. Biotechnology (Part – I) (Semester – II) Examination, 2015
Paper No. – I : CELL BIOLOGY
Day and Date : Thursday, 16-4-2015
Max.Marks : 70
Time : 11.00 a.m. to 2.00 p.m.
Instructions : 1) Part – I, question 1 is compulsory.
2) Attempt any four questions from Part – II.
3) Figures to the right indicate full marks.
4) Answers to the Part – I and Part – II are to be written in same
answer booklet only.
PART – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives :
1) _____________ has the cell wall.
a) Plant cell
b) Animal cell
c) Algal cell
7
d) Virus
2) Animal cells are
a) Totipotent
b) Pluripotent c) Both a and b d) Nullipotent
3) The type of membrane transport which uses energy is
a) Passive
b) Active
c) Diffusion
4) The cell organelle responsible for apoptosis is
a) Endoplasmic reticulum
d) Osmosis
b) Golgi bodies
c) Mitochondria
d) Ribosome
5) _____________ is a motor protein.
a) G-protein
b) Cyclin
c) Dynien
6) Segregation of chromatids takes place in
d) Kinase
a) Prophase
b) Metaphase c) Telophase
d) Anaphase
7) Hormones are involved in _____________ type of cell signaling.
a) Paracrine
b) Autocrine
c) Both a and b
d) Syncrine
P.T.O.
*SLRKP68*
SLR-KP – 68
B) Definitions :
1)
2)
3)
4)
7
Vacuole
Porins
Cis Golgi
Myosin
5) Gap junction
6) GPCR
7) Karyokinesis
PART – II
Answer any four of the following :
2. Explain in detail the organelles of eukaryotic cell.
14
3. Write a detailed note on structure and function of cell membrane.
14
4. Add a note on microtubules and microfilaments with functions.
14
5. Write a note on different types of cell division.
14
6. Answer any two from the following :
14
a) Write a note on organization of prokaryotic cell.
b) Explain the role of Golgi bodies in intracellular trafficking.
c) Add a note on molecular events in cell cycle.
7. Write short notes on (any two) :
a) Cell adhesion and extracellular matrix.
b) Signal transduction pathway for glucose regulation.
c) Hedgehog pathway.
___________
14
SLR-KP – 69
*SLRKP69*
Seat
No.
M.Sc. (Part – I) (Sem. – II) Examination, 2015
BIOTECHNOLOGY
Paper – II : Enzyme Technology
Day and Date : Saturday, 18-4-2015
Time : 11.00 a.m. to 2.00 p.m.
Max. Marks : 70
Instructions : 1) All question of Part – I are compulsory.
2) Answer any four questions from Part – II.
3) All questions carry equal marks.
4) Draw neat and labelled diagrams wherever necessary.
PART – I
1. A) Rewrite the following sentences by choosing the most correct alternative
given below :
7
i) The catalytic activity of enzyme is restricted to its small portion called as
______ site.
a) passive
b) active
c) allosteric
d) regulatory
ii) Reactions in which one or more products are released before all
substrates have been added are known as _____ reactions.
a) ordered sequential
b) random sequential
c) theorell-chance
d) ping pong
iii) The graphical representation of effect of temperature on activity of
enzyme gives _____ shape.
a) bell
b) parabolic
c) hyperbolic
d) sigmoidal
P.T.O.
SLR-KP – 69
-2-
*SLRKP96*
iv) ___________ is the activity of an enzyme per milligram of total protein.
a) specific activity
b) turnover number
c) enzyme activity
d) percent purity
v) The substrate for the lysozyme enzyme is
a) lysosomes
b) peptide chain
c) nucleic acids
d) bacterial cell wall
vi) For allosteric enzymes the substrate concentration against velocity gives
__________ curve.
a) parabolic
b) hyperbolic
c) sigmoidal
d) ellipsoidal
vii) __________ is the enclosing of a droplet of solution of enzyme in a
semipermeable membrane capsule.
a) Adsorption
b) Covalent bonding
c) Cross linking
d) Encapsulation
B) Define the following terms :
7
i) Abzymes
ii) Km
iii) Specific activity
iv) Hill’s plot
v) Metabolic engineering
vi) General acid catalysis
vii) Cooperativity.
PART – II
Answer any four questions from the following :
2. Write an essay on enzyme engineering.
14
3. Describe the different types of inhibitors of enzyme with their action and
experimental determination.
14
*SLRKP96*
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4. Give the IUB nomenclature system and classification of enzyme with at least two
examples from each class.
14
5. Describe in brief the various ways of enzyme regulation.
14
6. Answer any two of the following :
14
a) Write a note on ribozymes.
b) Explain the structural and functional relationship of ribonuclease.
c) Write in brief about biosensors.
7. Answer any two of the following :
a) Discuss the different graphical procedures used in enzymology with their
advantages and disadvantages.
b) Describe the structural and functional relationship of aspartate
transcarbamoylase.
c) Write a note on allosteric enzymes and their modulators.
–––––––––––––––
14
SLR-KP – 70
*SLRKP70*
Seat
No.
M.Sc. Biotechnology (Part – I) (Semester – II) Examination, 2015
Paper No. – III : MOLECULAR CELL PROCESSING
Day and Date : Tuesday, 21-4-2015
Total Marks : 70
Time : 11.00 a.m. to 2.00 p.m.
Instructions : 1)
2)
3)
4)
5)
Section – I is compulsory.
From Section – II attempt any four.
All questions carry equal marks.
Figures to the right indicate full marks.
Draw neat and labeled diagrams.
SECTION – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives :
7
1) How many stop codons are there in the genetic code ?
a) 2
b) 3
c) 4
d) None of these
2) A “TATA Box” is
a) the translation termination sequence
b) an important base sequence in the promoters of bacteria
c) the site where the RNA polymerase II binding complex is assembled
d) an example of one of the translation stop codons
3) The amino acid sequence of a polypeptide chain comprises the _________
structure of the protein.
a) primary
b) secondary
c) tertiary
d) quaternary
P.T.O.
SLR-KP – 70
*SLRKP70*
-2-
4) _________ RNA required for protein synthesis.
a) tRNA
b) mRNA
c) rRNA
d) all of these
5) The type of mutation most commonly associated with exposure to UV light is
a) thymine dimerization
b) base deamination
c) depurinization
d) a base deletion
6) _________ sub unit act as a clamp during DNA replication.
a)
b)
c)
d)
7) Sigma factor is a component of ________
a) DNA ligase
b) DNA polymerase
c) RNA polymerase
d) Endonuclease
B) Definitions :
7
1) Polyadenylation
2) Transcription factors
3) Foot printing
4) Topoisomer
5) –10 and –35 regions in gene
6) Klenow fragment
7) Holiday intermediate.
SECTION – II
Answer any four of the following :
2. Explain the process of prokaryotic DNA replication with neat labeled diagram
and add a note on proof reading mechanism.
14
3. Explain the mechanism of post translation modifications and add a note on protein
folding.
14
4. What are ribosomes ? Explain the process of translation in prokaryote with neat
labeled diagram.
14
*SLRKP70*
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5. Write a note on nucleotide excision and mismatch repair with neat labeled diagram.
14
6. Answer any two from the following :
14
a) Explain the eukaryotic RNA polymerases.
b) Explain the recombination process.
c) Write a note on post transcription modification of eukaryotic mRNA.
7. Write short notes on (any two) :
a) E.Coli DNA Pol I.
b) Genetic code
c) Promoter elements, Activators, Enhancers and repressors.
_______________
14
SLR-KP – 71
*SLRKP71*
Seat
No.
M.Sc. Biotechnology (Part – I) (Semester – II) Examination, 2015
Paper – IV : IMMUNOLOGY AND IMMUNO TECHNIQUES
Day and Date : Thursday, 23-4-2015
Time : 11.00 a.m. to 2.00 p.m.
Instructions : 1)
2)
3)
4)
Max. Marks : 70
Part – I, Question 1 is compulsory.
Attempt any four questions from Part – II.
Figures to the right indicate full marks.
Answers to the Part – I and Part – II are to be written in
same answer booklet only.
PART – I
1. A) Select appropriate answer from the following and rewrite :
7
1) ________ discovered gene rearrangement in antibody production for which
he received noble prize in 1987.
a) Susuma Tonegawa
b) Emil Von Behring
c) Max Theiler
d) Elie Metchnikoff
2) __________ is a group of protein that circulate in an inactive state, further
converted to active state by immunological mechanisms that enables
them to damage the membrane of pathogenic organisms.
a) Interferon
b) Lysozyme
c) Complement
d) Phagosomes
3) Main genetic region of the major histocompatibility complex in house is
located on _________ chromosomes.
a) 17
b) 10
c) 6
d) 8
4) __________ will not induce antibodies by its own but will react in vitro with
antibodies formed to a conjugate with an immunogenic carrier.
a) Mitogens
b) Epitopes
c) Haptens
d) Globular proteins
P.T.O.
*SLRKP71*
SLR-KP – 71
5) __________ nanoparticles have been used in a variety of vaccines.
(diphtheria, pertussis, tetanus, polio etc.). It has been found to induce a
predominantly TH1 response.
a) Zinc oxide
b) Alum
c) Silver
d) Calcium phosphate
6) MHC Class I molecules are synthesized and loaded with peptides in the
a) golgicomplex
b) E. R.
c) plasma membrane
d) cytosol
7) ELISA is serodiagnostic assay whose sensitivity is
a) milligrams b) pictograms c) nanograms
d) micrograms
B) Definition :
1) SARS
2) Agglutination
3) Immunosuppressant
4) Tumor antigen
5) Cytokines
6) Epitopes
7) Clonal selection.
7
PART – II
Answer any four of the following :
2. Give an account on mechanism of antibody production against T1 and TD antigen.
14
3. Write an essay on MHC genetic organization and inheritance.
14
4. Give a brief account on peptide vaccine.
14
5. Give a principle application features of Immunoelectrophoresis and agglutination. 14
6. Answer any two from the following :
A) Autoimmunity
B) Rabies
C) Complement activation pathways
14
7. Write short notes on (any two) :
A) Give an account of components of humoral immunity and CMI.
B) Short note on cancer immunotherapy.
C) State the principle and various application of ELISA.
14
_______________
SLR-KP – 76
*SLRKP76*
Seat
No.
M.Sc. (Part – II) (Semester – III) (New) Examination, 2015
BIOTECHNOLOGY
Paper – I : Advanced Analytical Techniques (C.G.P.A. Pattern)
Day and Date : Wednesday, 15-4-2015
Max. Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
N.B. : 1)
2)
3)
4)
5)
Section – I is compulsory.
From Section – II attempt any four.
All questions carry equal marks.
Figures to the right indicate full marks.
Draw neat and labelled diagram.
SECTION – I
1. A) Complete the sentences by selecting correct answer from given alternatives :
7
i) The tracking dye used in electrophoresis is
a) Bromophenol blue
b) Blue dextran
c) Phenol red
ii) The value of Rf is always
a) Less than 1
c) Equal to 1
d) Neutral red
b) Greater than 1
d) + or – 1
iii) Radioactively labelled and unlabelled antigens are used in
a) Flowcytometry
c) ELISA
b) AAS
d) RIA
iv) The time required for the analyte peak to appear is known as
a) Void volume
b) Flow rate
c) Retention time
d) Chromatography
v) The unit energy of an electromagnetic radiation is called as
a) Frequency
b) Wavelength
c) Photon
d) Ohm
P.T.O.
*SLRKP76*
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vi) CD and ORD are frequently used in studies on
a) Fats
b) Proteins
c) Carbohydrates
d) Hydrocarbons
vii) In _____________ type of microscopy the background is dark with refractile
bodies.
a) Dark field
b) Fluorescence
c) Phase contrast
d) Bright field microscopy
B) Define the following :
i) Numerical aperture
ii) rpm
iii) Theoretical plates (column chromatography)
iv) Native get
v) Excitation
vi) Lasers
vii) Electrode.
7
SECTION – II
Attempt any four :
2. What are the properties of an electromagnetic radiation ? Give the consequences
of interaction of electromagnetic radiation with matter.
14
3. State the principle and working of a Geiger Muller counter.
14
4. Give the principle working and applications of 2-D gel electrophoresis.
14
5. Explain in detail the different types of paper chromatography.
14
6. Write short notes on (any two) :
A) Ultracentrifugation
B) Fluorescence microscope
C) Pulse Field Gel Electrophoresis.
14
7. Answer any two :
A) CD and ORD
B) Immunoblotting
C) Size exclusion chromatography.
___________
14
SLR-KP – 77
*SLRKP77*
Seat
No.
M.Sc. (Part – II) (Semester – III) Examination, 2015
BIOTECHNOLOGY (CGPA Pattern) (New)
Paper – II : Fermentation Technology
Day and Date : Friday, 17-4-2015
Max. Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1) All questions of Section I are compulsory.
2) Answer any four questions from Section II.
3) All questions carry equal marks.
4) Draw neat and labelled diagrams wherever necessary.
SECTION – I
1. A) Rewrite the following sentences by choosing the correct alternative given
below :
1) Aeration is bioreactor is provided by
a) Impellers
c) Sparger
7
b) Baffles
d) All of these
2) Increasing the stirred speed improve the value of
a) Reynold number
b) Power number
c) Mixing time
d) KLa
3) Precipitation of proteins by increasing the ionic strength of the solution is
popularly known as
a) Salting in
b) Salting out
c) Iso-electric precipitation
d) Desalting
4) Lag phase in batch culture is also called
a) time of growth
b) time of cell division
c) time of adaptation
d) all of these
5) ___________ is used as antifoam.
a) Oils
b) Silicon oils
c) Both
d) None of these
P.T.O.
*SLRKP77*
SLR-KP – 77
6) Corn steep liquor is the product of
a) Corn starch
b) Sucrose
c) Dairy industry d) None of these
7) The cell number or biomass of batch culture exhibits curve of
a) J shape
b) C shape
c) S (sigmoidal) d) None of these
B) Define the following terms :
7
1) Primary metabolites
2) Antifoam agents
3) Strain improvement
4) Process validation
5) Biosensor
6) Immobilization
7) Batch cultivation.
SECTION – II
Answer any four :
2. Describe methods used for gene manipulation in microorganism.
14
3. Define bioreactor. Give types of bioreactor in detail.
14
4. Give an account on methods of protein extraction and purification.
14
5. Give an account of fermentation media.
14
6. Answer any two of the following :
14
a) Write an short note on methods of air sterilization.
b) Give an brief account on computer application in fermentation technology.
c) Give the methods of effluent treatment.
7. Answer any two of the following :
a) Short note on microbial production of enzymes.
b) Short note on bacterial culture collection units.
c) Short note on aqueous two phase separation of fermented products.
–––––––––––––––––––
14
SLR-KP – 78
*SLRYKP78*
Seat
No.
M.Sc. (Biotechnology) (Part – II) (Semester – III) (CGPA Pattern)
Examination, 2015
Paper – III : RESEARCH METHODOLOGY AND IPR (New)
Day and Date : Monday, 20-4-2015
Total Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1)
2)
3)
4)
5)
Section I is compulsory.
From Section II attempt any four.
All questions carry equal marks.
Figures to right indicate full marks.
Draw neat and labelled diagrams.
SECTION – I
1. A) Complete the sentences by selecting correct answer from the given alternatives :
7
1) You should use _________ in all contents in the manuscript.
a) Single space b) Double space c) No space
d) None
2) Quantitative research is also called ________ research.
a) Conclusive
b) Descriptive
c) Both a) and b)
d) None
3) Which of the following is a major method of data collection ?
a) Questionnaires
b) Secondary data
c) Interviews
d) All
4) Research that is done to understand an event from the past is known as
a) Experimental Research
b) Historical Research
c) Replication
d) Archival Research
5) _________ of the following is not specifically protected by intellectual
property legislation.
a) Trademarks
b) Patents
c) Copyright
d) Trade secrets
6) _________ are geographical indications in India.
a) Mysore Sandal Soap
b) Solapur Chaddar
c) Kolhapuri Chappal
d) All
7) ________ biological material is granted patent in India.
a) Basmati rice
b) Turmeric
c) Both a) and b)
d) None
P.T.O.
*SLRYKP78*
SLR-KP – 78
B) Define the following :
i) Hypothesis
ii) Proportion
iii) Correlation Co-efficient
iv)
v)
vi)
vii)
7
Regression
Trade secrets
UPOV
Author instructions for journal.
SECTION – II
Attempt any four :
2. Give the different guidelines for introduction and materials and methods in the
preparation of manuscript.
14
3. What is data collection ? Explain in detail the different methods of data collection.
14
4. Explain Chi-square tests and its applications.
14
5. What is sample size ? Explain in detail types of sampling.
14
6. Answer any two :
14
a) Write a note on plant variety protection in India.
b) Explain types of patenting.
c) Preparation of Oral Presentation and Poster Presentation for conferences.
7. Write short notes on (any two) :
a) WIPO
b) Basmati rice
c) Computer and internet application in Research.
_____________
14
SLR-KP – 79
*SLRKP79*
Seat
No.
M.Sc. II (Semester – III) Examination, 2015
BIOTECHNOLOGY
Paper – IV : Plant Biotechnology (New-CGPA Pattern)
Day and Date : Wednesday, 22-4-2015
Max. Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1)
2)
3)
4)
5)
Section I is compulsory.
From Section II attempt any four.
All questions carry equal marks.
Figures to right indicate full marks.
Draw neat and labelled diagrams.
SECTION – I
1. Rewrite the following sentences by using correct alternative.
7
1) ______________ is the unorganized proliferative mass of cell.
a) Callus
b) Cross
c) Totipotency
d) Meristem
2) ______________ is the father of tissue culture.
a) Hanning
b) Skoog
c) Haberlandt
d) Pfizer
3) The part of a plant used for culturing is called
a) Callus
b) Scion
c) Stock
d) Explant
4) Ethylene is employed for
a) Ripening of fruit
c) Increasing height
b) Stimulation of cell division
d) All of the above
5) ______________ is the variations occur in plants during plant tissue culture.
a) Somatic hybrid
b) Cybrid
c) Somaclonal variation
d) Callus
6) ______________ is the preservation of germplasm at – 196°C temperature.
a) Variation
b) Cryopreservation
c) Salting
d) Recombination
P.T.O.
*SLRKP79*
SLR-KP – 79
7) ______________ is indirect gene transfer method.
a) Agrobacterium mediated gene transfer
b) Microinjection
c) Electroporation
d) Particle bombardment
B) Answer the following terms :
7
1) Rhizoenesis
2) Embryoids
3) Totipotency
4) Protoplast
5) Seed Bank
6) Vectors
7) Secondary metabolite.
SECTION – II
Attempt any four :
2. Explain the Agrobacterium mediated gene transfer mechanism in plants.
14
3. Explain the different methods of protoplast fusion.
14
4. Give an detail account on somatic embryogenesis.
14
5. Explain in detail the plant hormone types and mechanism of action in plant growth.
14
6. Answer any two of the following :
14
1) Write a note on somaclonal variation.
2) Write a note on edible vaccine as secondary metabolite products.
3) Describe in detail micropropagation as rapid clonal propagation.
7. Answer any two of the following :
1) Explain types of somatic hybrids formation.
2) Write a note on biodegradable plastic.
3) Explain the particle bombardment method for gene transfer.
–––––––––––––––
14
SLR-KP – 84
*SLRKP84*
Seat
No.
M.Sc. (Part – II) (Semester – IV) Examination, 2015
BIOTECHNOLOGY
(C.G.P.A. Pattern) (New)
Animal Biotechnology (Paper – I)
Day and Date : Thursday, 16-4-2015
Total Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1)
2)
3)
4)
Part – I, Question 1 is compulsory.
Attempt any four questions from Part – II.
Figures to the right indicate full marks.
Answers to the Part – I and Part – II are to be written in
same answer booklet only.
PART – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives :
7
1) The process of bubbling gas through the culture is referred to as
a) aeration
b) sparging
c) diffusion
d) oxygenation
2) ________ of the cells represents the capability of their existence, survival
and development.
a) viability
b) survival
c) senescence
d) aging
3) A foreign DNA that is introduced during transgenesis is called
a) Oncogene
b) Cistronic gene
c) Transgene
d) Transfection gene
P.T.O.
SLR-KP – 84
*SLRKP84*
-2-
4) Culture media are sterilized by autoclaving at _________ psi that gives
121°C for __________ min.
a) 10, 20
b) 15, 30
c) 20, 30
d) 20, 50
5) ________ are obtained either from transformed cell lines in vitro or
cancerous cells.
a) Finite cell lines
b) Suspension cell lines
c) Primary cell lines
d) Continuous cell lines
6) Commonly, animal cells are centrifuged at ______
a) 15°C
b) 20°C
c) 30°C
d) 40°C
7) Two important enzymes used in tissue disaggregation are _______ and
______
a) Collagenase and trypsin
b) Cellulase and trypsin
c) Cellulase and collagenase
d) Amylase and cellulose
B) Definitions :
1) Cell lines
2) Cell cloning
3) Synthetic media
4) Open cultures
5) Contact inhibition
6) Suspension cultures
7) Established cell lines.
7
*SLRKP84*
-3-
SLR-KP – 84
PART – II
Answer any four of the following :
2. Give an account of commercial applications of animal cell culture.
14
3. Explain the method of production of transgenic animals. Add a note on their
applications.
14
4. What is cell hybridization ? Explain it with an example. Add a note on its
applications.
14
5. Describe the working of a commercial laboratory with respect to aseptic techniques
and control of contamination.
14
6. Answer any two from the following :
14
1) Describe in brief primary culture and explant culture.
2) Describe in detail the cell senescence.
3) Describe the natural media used for animal cell culture.
7. Write short notes on (any two) :
1) Laminar flow hoods
2) Cytotoxicity
3) Organ culture.
_______________
14
SLR-KP – 85
*SLRKP85*
Seat
No.
M.Sc. (Part – II) (Semester – IV) Examination, 2015
(Paper No. – II) : BIOTECHNOLOGY (New CGPA Pattern)
Industrial and Environmental Biotechnology
Day and Date : Saturday, 18-4-2015
Max. Marks : 70
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1)
2)
3)
4)
All questions of Section I are compulsory.
Answer any four questions from Section II.
All questions carry equal marks.
Draw neat and labelled diagrams wherever necessary.
SECTION – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives :
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1) _____________ is secondary metabolite.
a) Alcohol
b) Amino acid
c) Antibiotics
d) Organic acid
2) Industrial production of Acetone-Butanol by microorganism process was
started in
a) Brazil
b) United State
c) England
d) Europe
3) Sucrose is used as a best source of carbon in ___________ production.
a)
b) Sulphuric acid
c) Citric acid
d) Acetic acid
P.T.O.
SLR-KP – 85
*SLRKP85*
-2-
4) _________ fungal species is important in fungal biomass production.
a) Rhizobium loti
b) Penicillium notatum
c) Trichoderma viride
d) Fusarium oxysporum
5) The correct sentence about rennet is that it
a) is used in production of cheese
b) is complex enzyme produced in stomach of ruminant mammal
c) shows presence of chymosin
d) all of these
6) Vermicomposting is __________ type of solid waste treatment.
a) aerobic treatment
b) anaerobic treatment
c) both a) and b)
d) chemical treatment
7) Empirical equation is derived by ___________ in baker’s yeast production.
a) J. S. Harrison
b) M. S. Harrison
c) Louis Pasture
d) Michelis and co-worker
B) Define the terms :
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1) Fermentation
2) Bio-surfactant
3) Metabolite
4) Bioethanol
5) Rennet
6) Hydroxylation
7) Antibiotic.
SECTION – II
Answer any four of the following :
2. Explain role of enzymes involved in meat and dairy industry.
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3. Define secondary metabolite and write note on antibiotic synthesis of penicillin. 14
4. Illustrate the technical production and uses of nucleotide.
14
*SLRKP85*
-3-
SLR-KP – 85
5. Write an account of applications of enzyme in cereal extraction, fruit processing
and grain processing.
14
6. Answer any two from the following :
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a) Explain the basic aspect of solid waste management.
b) Discuss the microbial production of glutamic acid.
c) Describe the algal biomass production.
7. Write short notes on (any two) :
a) Brewing processing.
b) Celluloses and hemicelluloses.
c) Secondary products of filamentous fungi.
___________
14
SLR-KP – 86
*SLRKP96*
Seat
No.
M.Sc. (Part – II) (Sem. – IV) (CGPA Pattern) Examination, 2015
BIOTECHNOLOGY
Paper – III : Stem Cell Technology and Regenerative Medicine (New)
Day and Date : Tuesday, 21-4-2015
Time : 3.00 p.m. to 6.00 p.m.
Max. Marks : 70
Instructions : 1) Section I is compulsory.
2) From Section II attempt any four.
3) All questions carry equal marks.
4) Figures to right indicate full marks.
5) Draw neat and labelled diagrams.
SECTION – I
1. A) Rewrite the following sentences by using correct alternative.
7
1) In animal tissue culture, use of tripsin for generation of replicate subcultures
is introduced by
a) Dulbecco
b) Harrison
c) Daenall
d) Butler
2) ___________ CO2 concentration is required for culturing animal cell.
a) 2 – 5%
b) 1 – 10%
c) 10 – 15%
d) 15 – 20%
3) ________ factor does not involve in replacement of culture media.
a) Morphological deterioration
b) Cell type
c) Drop in pH
d) Mode of growth
4) Phenol red indicator used in animal tissue culture is shows red colour at pH
a) 7
b) 7.4
c) 7.8
d) 7.6
P.T.O.
SLR-KP – 86
*SLRKP96*
-2-
5) Embryonic stem cells are
a) Unipotent
b) Oligopotent
c) Multipotent
d) Totipotent
6) The animal extracellular matrix contain
a) Only interstitial matrix
b) Only basement membrane
c) Interstitial matrix and basement membrane
d) Cell wall components
7) __________ is not a source of stem cell.
a) Umbilical cord blood
b) Bone marrow
c) Early embryo
d) Sperms and egg
B) Answer the following terms :
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1) Cell Adhesion
2) Metabolism
3) Sterilization
4) Stem cell
5) Bioreactor
6) Transplantation
7) Tissue engineering.
SECTION – II
(Attempt any four)
2. Give a detail account on types of tissue culture and its advantages.
14
3. Explain in detail different types of stem cell.
14
4. Write an essay on media composition for animal cell culture.
14
5. Explain in detail clinical application of stem cell.
14
*SLRKP96*
-3-
SLR-KP – 86
6. Answer any two of the following :
14
1) Explain types of sterilization methods.
2) What are the different substrate material used for animal cell culture ?
3) Give general account of isolation and characterization of stem cells.
7. Answer any two of the following :
1) Explain in detail immuno isolation techniques.
2) Write a detail account on design of bioreactor for stem cell.
3) Explain regeneration of bone using stem cell.
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14
SLR-KP – 87
*SLRIKP87134*
Seat
No.
M.Sc. (Part – II) (Semester – IV) Examination, 2015
BIOTECHNOLOGY (C.G.P.A. Pattern) (New)
Genetic Engineering (Paper – IV)
Day and Date : Thursday, 23-4-2015
Time : 3.00 p.m. to 6.00 p.m.
Instructions : 1)
2)
3)
4)
Max. Marks : 70
Part – I, Question 1 is compulsory.
Attempt any four questions from Part – II.
Figures to the right indicate full marks.
Answers to the Part I and Part – II are to be written in same
answer booklet only.
PART – I
1. A) Rewrite the sentence after choosing the correct answer from the given
alternatives.
i) ___________ is the source of enzyme exonuclease I.
a) B. substilis
b) E. coli
c) Salmonella typhi
d) Aspergillus niger
ii) pUC plasmid vector developed by
a) Smith
b) Harrison
c) Joachim Messing
d) Rutherford
iii) Lambda phage is a ________ kb vector.
a) 12
b) 25
c) 35
d) 48.5
iv) __________ plasmid is a commonly used plant vector.
a) Ti
b) Bi
c) Si
d) Ai
v) __________ analysis has been successfully applied for epidermiology of
Listera monocytogen.
a) Spectroscopic
b) RAPD
c) DNA Sequencing
d) Protein Blotting
vi) Dideoxy chain termination method is also referred as _________
a) Plus
b) Minus
c) Neutral
d) Plus-Minus
vii) Restriction endonucleases were discovered by
a) Smith
b) Murashigue
c) Carrel
d) Haberlandt
7
P.T.O.
*SLRIKP87134*
SLR-KP – 87
B) Define :
a) Cosmid
b) Southern Blotting
c) Gene Therapy
d) Bio-pharming.
e) Ligases
f) Probes
g) Exonucleases.
7
PART – II
Answer any four of the following :
2. Describe the method of isolation and purification of donar DNA.
14
3. Explain in detail types of microarray.
14
4. Explain in detail manufacturing of genetically engineered biotherapeutics and
vaccines.
14
5. Discuss Maxam-Gilbert DNA sequencing method.
14
6. Answer any two of the following :
14
a) Give brief account on Yeast as a expression vector.
b) Give brief account on Ligases.
c) Discuss RNA blotting.
7. Write short notes on (any two) :
a) Discuss immuno-screening method for selection of recombinants.
b) Explain DNA transfer method with respect to Electroporation.
c) Write a short note on cosmid as a cloning vector.
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