BIOSAFETY LABORATORY INSPECTION FORM PRINCIPAL INVESTIGATOR: AUDITOR: DEPARTMENT: BUILDING & ROOM(S): LAB REP: Y N N/A DATE: PHONE: EMAIL: ITEM Biosafety Resources and Documentation COMMENTS http://ehs.sc.edu/Biohaz.htm Biological research (rDNA, BSL-2 agent, human materials, etc.) is approved by IBC Biosafety application is current and amendments have been approved by IBC Biosafety Manual is readily available Biological Spill Procedures are available and personnel are familiar with procedures Pest Control Policy is available and no pest control problems observed Biosafety Training is documented for all personnel Laboratory Facilities http://www.cdc.gov/od/ohs/biosfty/biosfty.htm Laboratory has a sink for hand washing Eyewash station is readily available Lab designed so that it can be easily cleaned (i.e. no carpet, cloth furniture, etc.) Bench tops are impervious to water and disinfectant use Housekeeping is appropriate and lab is maintained in a clean/sanitary condition Lab windows that open to the outside are fitted with fly screens Safety Equipment – Primary Barriers & PPE http://ehs.sc.edu/Biohaz.htm Protective clothing (i.e. lab coat) worn to prevent contamination of personal clothing Gloves are worn to protect hands from exposure to hazardous materials Protective eyewear worn when potential to create splashes from samples Biological safety cabinets (BSCs) are used whenever procedures with potential for creating infectious aerosols or splashes are conducted BSCs located away from doors, heavily traveled areas, and other airflow disruptions BSCs have been certified within the past year (annual certification) Standard Microbiological Practices http://www.cdc.gov/od/ohs/biosfty/biosfty.htm Work surfaces are decontaminated after completion of work or after any spill All cultures, stocks, and other biological waste is properly disposed (i.e. autoclaved, chemical disinfection, EHS pickup) Used needles, syringes, and other sharps placed in appropriate sharps container Needles are never bent, broken, recapped or reused before disposal Plasticware is substituted for glass whenever possible Access to the laboratory is limited to authorized lab staff whenever possible Eating and drinking and the storage of food are prohibited in the laboratory BSL-2 Labs (Special Practices) http://www.cdc.gov/od/ohs/biosfty/biosfty.htm Standard Operating Procedures (SOP) available for each BSL-2 agent used/stored A lab-specific biosafety manual has been prepared and is accessible Biohazard signage posted on lab entrance and appliances storing BSL-2 agents Vacuum lines are protected with liquid disinfectant or HEPA filters Samples placed in durable, leak proof container during storage and transport All animals and plants not associated with work being performed are prohibited Bloodborne Pathogens (i.e. human blood, body fluids, cell lines, unfixed tissues) http://ehs.sc.edu/OccHealth.htm Exposure Control Plan is accessible All personnel have completed annual BBP training All personnel have been offered the Hepatitis B vaccination Personnel are familiar with post-exposure evaluation and follow-up Engineering and work practice controls are used to reduce the risk of exposure Shipping Biological Materials http://ehs.sc.edu/shipping.htm Personnel have completed biological shipping training in the past two years Comments: Biological materials used/stored: All items must be corrected within 2 weeks of inspection; otherwise, please provide EHS with a corrective action plan within 2 weeks. ( Lab Rep. initials →) EHS Office: (803) 777-5269 Fax: (803)777-5275 Laboratory Approved for: Closed: EHS-F-100 ______________________ ______________________ ______________________ BSL-1 BSL-2 Page 1 Destroy Previous Revisions Issue Date: 10/13/07 Approved: MAR