AUSTRALIAN HOP MARKETERS PTY. 1979 Research Report LTD AUSTRALIAN BOP MARKETERS PTY. LTD. RESEARCH REPORT 1979. PREFACE: This Research Report documents part of the work in hops research conducted by Australian Hop Marketers Pty. Ltd. over the past year and outlines that work proposed for the coming year. Most of the papers included were initially written as internal papers and tend to emphasise costs and commercial problems, rather than academic and technical matters. The report indicates something of the efforts being made by Australian Hop Marketers Pty. Ltd. to improve yields and returns to hop growers. I hope it will also be of benefit to Brewers interested in reducing freight costs and storage alpha acid loss and the improvement of total alpha acid and hop quality. M.G. CLEARY. GENERAL MANAGER. AUSTRALIAN HOP MARKETERS PTY. LTD. CONTENTS Research Programs 1979/'80. Foliar Fertilizers, Report on the 1978/'79 PAGE 1 8 Trial. Substitution of Soil-Applied Nitrogen with 10 Foliar Sprays. Hormone Applications, Report on the 1978/'79 21 Trials. Report on Miscellaneous Experiments, 29 Bushy Park Hop Nursery - 1979. Antioxidants,Materials and Trials. 41 Vacuum Packed Pressed Hops, 1979 Report. 45 Vacunm Packing Pressed'Hops at High Density. Whole Hop Pellets - 1979 Report. Hop Growing in Tasmania. Some Notes on 49 57 64 Cultural Practices and Timing of Operations. AUSTRALIAN HOP MARKETERS PTY. LTD. HOPS RESEARCH 1979/80 I.J. Edwards P.J. Versluys SUMMARY: A proposed hops research progranme is presented for 1979/80. It consists of many routine and continuing experiments together with the exploration of some new ideas which have a high risk, but if successful could significantly improve the economics of hop growing in Australia. These include a high intensity, low budget, random breeding progranme, extensions of 1979 low trellis experiments to include novel picking and cultural techniques, trials to extend the harvest season with chemical sprays and seme new propagation techniques. A.H.M. Research 1979. The following progranme of research is proposed for 1979/80. 1. Hop Breeding (IE) With the appointment of Grahane Hughes to Carlton Jones, and the sale of the Ringwood hop nursery by C.U.B., Australia no longer conducts any breeding research into hops. On the other hand other countries are rapidly advancing in this field with many conmercial varieties now about the same alpha as Pride fo Ringwood (e.g. Wye target 11%, Bullion 9-10%,) and experimental varieties around 15-16% alpha. Since hops are generally brewed directly in proportion to the alpha acid content, each 1% of alpha acid is worth approximately 25c/kg hops. Traditional hop breeding prograimes usually test 300 - 400 new varieties each year. Hops are selected from carefully controlled crosses and are tested for storage stability, disease resistance, alpha and beta acids, oils, vigour and time of maturity. The breed ing progranme is generally expensive because of the labour involved in monitoring all crosses, germinating seeds, transplanting seedlings two to three times, and analysing many parameters. From discussions with hop breeders (P. Versluys, G. Hughes) and observation of the new varieties arising fran breeding programmes, it is apparent that hop breeding is more a matter of chance, than of technique i.e. that there is no systematic method known of crossing (apart from seme basic rules) that increases the liklehood of a new successful variety arising. What is proposed is that AHM, as part of its general research progranme, plant a large number of seeds of Pride of Ringwood hops, selected from a variety of sources to maximise the genetic variability (from the male parent) and screen them primarily for alpha acid i.e a breed ing progranme concentrating on cheapness, high numbers, and a single parameter (alpha). Tne logic is based on the premise that a desirable variety has a low probability of emerging, but that that probability will be increased in proportion to the number of seedlings screened, and not at all in preparation to the care taken in selecting crosses. It is envisaged that from a breeding progranme costing $5,000/year in analysis , materials and labour (mainly analysis) about 10,000 seed ling/year will be screened for alpha. This will represent the number of progeny tests arising fron about twenty years of conventional breed ing and, with a high alpha parent to begin with, should show a real chance of producing a 167<> alpha acid variety with acceptable vigour, yield, flavour and storage stability characteristics. 2. Low Trellis (IE) Tne small experimental area of low trellis yeilded up to 860kg/acre for second year plants in 1979, with large cones formed virtually to ground level. The yield would be expected to increase further for third year plants, and for closer row spacings. In the low trellis vines reach the wirework in December and then grow under (80% of vines) or over the topwire and continue growing. The growing tip is then subject to wind damage by being dashed against the wire and adjacent vines. Wind also moves the vines in the direct ion of the prevailing wind direction, and they readily become tangled in winds not strong enough to destroy the growing tip. If the growing tip is killed, strong laterals emerge from the two to four topmost pairs of buds, and these are also inclined to tangle. Tangling of vines could be reduced by planting rows at right angles to the prevailing westerly winds; also by increasing the separation between strings, (currently 2') and by using a double top wire, rather than a single. With this arrangement the vines could be harvested more or less in the conventional manner using a car trailer and with a man in front cutting the top and base of the vines. This system would have substantial advantages over high wirework in cost of establishment (probably $1,000.00/acre) but not in management and harvesting. To utilize the low trellis to its full premise, it would be desirable to let the new vines grow up old vines for self training and then picking without removing vines, i.e. a system with advantages: (a) (b) (c) (d) Self training with no need for string (saving $200/acre off present system). Dense canopy with intertwining for full interception of sun light and theoretically greater yield (10% yield is worth $200/acre) Harvesting of leaves and cones only, with full potential for food reserves in vines to return to rootstock, thereby enhancing yield in next year. Harvesting by blowing or sucking off cones and leaves, with later separation in existing stationary picking machines. Potential saving $100 - $200/acre. The most difficult aspect of this system would be the development of a suitable picking machine. Development of mobile harvestors for normal trellis has been going on for many years by top engineers with only limited success. This proposed system simply blows off the cones and leaves, and these can be routinely separated in exisiting machines. Although simple in principle, a harvestor relying onour movement to dislodge cones and leaves has not been built, and may not prove feasible. It is considered worth building a small experimental machine though, since this could be constructed for $1,000 to $2,000, and could be applicable to other crops. Chemicals for Increasing Alpha Acid (IE) Alpha is influenced in an unknown way by soil nutrients. A pre liminary trial in 1979 indicated that chemicals applied as foliar sprays can have dramatic effects (mainly detrimental) on alpha. Although the mechanics of any effects on alpha are unknown, applic ation of foliar sprays is easily done and could readily become a routine operation if consistent positive effects on alpha could be demonstrated. For this reason it is proposed to further investigate the possibilities of increasing alpha by applied chemicals. Trials will involve applications of a range of chemicals, at differing times and concentrations. Pellet Plant Improvements (IE) A continuing programme of experiments at the pellet plant has the following aims:1. Increasing throughput, without concurrently increasing die temp erature, increasing alpha loss and lowering pellet density. 2. 3. Reducing alpha losses. Improving packaging at lower cost. This year the main aims are:- (a) To produce most pellets at V (6.3 mm) diameter rather than 3/16" (4.8 nm). The 6 nm pellets are much easier to manufacture than 5 mm but generally have poor density characteristics if produced from our current bronze die; or poor appearance if produced fron our steel die. A new bronze die has been ordered which should increase throughput without reducing density adversely. (b) Most alpha loss occurs between the hammer mill and the pellet die. The loss is caused by the hanmer milling, both by physical loss of resin built up in the body of the hanmer mill, and by oxidation in the air streamby the hanmer mill. Hanmer mill screens must also be frequently changed, and hence lower through put rates somewhat when alpha is high, as well as significantly raising the proportion of physical resin loss. With the new cyclone now installed for the whole hop pellets, there is a possibility of bypassing the hanmer mill altogether i.e. feeding intact hops into the pellet die. The commercial feasibility of this is to be examined bearing in mind pellet particle size, throughput, alpha loss reduction & pellet density. Alpha Losses (IE) Despite some documented drying losses in excess of IX (absolute) alpha acid, (and seme tentative gains by the University of Tasmania) we have little idea of the losses in picking, drying and pelleting for all situations and all growers. Since variation in alpha is so great, a large number of samples are necessary just to properly cbcument drying losses in any single kiln. The feasibility of properly monitoring drying losses in all kilns, and conducting miscellaneous experiments on possible alpha losses and gains hinges on economical analysis. Approval of the recent application for $10,000 for automatic alpha analysing equipment will greatly facilitate analysis of greater numbers of samples. Storage Trials (IE) Alpha losses are well documented for whole hops in standard bales and for hop pellets, but not for any of the other products manufact ured by AHM. It is proposed to set up a routine trial to test alpha losses in: (a) Vacuum packs of pressed hops (with and without vacuum loss) (b) Whole hop pellets (with and without vacuum loss) (c) High density bales. (d) Powder pellets and standard bales as control. Preliminary trials and theory indicate that the vacuum bales will lose less alpha than vacuum packed powder pellets. Losses in whole hop pellets are not known at all. Hormone Sprays (PV) After two years of trials, the potentials for increasing yields by selected hormone applications in unclear. In 1977-78 a large increase was recorded, whereas in 1978-79 the increase was very small. An associated effect though shows promise of making hormone applications worthwhile. This is the effect of some hormones (and some other chemicals) in delaying ripening. It is proposed to continue trials with hormones to further document possible yield increases and dates of maturity following hormone applications. Antioxidants (PV) Antioxidants are widely used in the food industry to prevent product deteoration by oxidation. Preliminary trials with hop pellets last year indicated that alpha losses could be reduced by antioxidants. It is proposed to extend this work to include other chemicals with antioxidant properties, and investigations of likely practical implications (technical problems and customer reactions). Soil Sampling (PV) All AHM growers receive fertilizer reconmendations based on soil testing. 1979 analyses have revealed some high phosphorus readings which are possibly due to low rainfall (with consequent low leaching) and high Ph (from high lime applications). There are some indications that these high Ph readings correspond to low alpha results. It is proposed to conduct trials to determine the effect of high phosphorus levels on alpha and yield. The work will involve applicat ions of mono potassium phosphate at various concentrations and, possibly, the monitoring of phosphorus movement within the plant using radioactive tracers. Foliar Fertilizers (PV) From trials conducted in the last two years, foliar fertilizer use can be generally reconmended for Tasmanian conditions. It is proposed to document the use of foliar fertilizers under Victorian conditions during 1979/80. Leaf Sampling (PV) Leaf analysis accurately measures the nutrients taken up by the plant and shows potential for determining fertilizer reconmendations and nutrient deficiencies,The leaf analysis though, varies throughout the growing season. Continuing routine sampling and analyses are proposed on six selected paddocks to monitor basic leaf data through the growing season. It is anticipated that one more years results will enable practical evaluation of the use of leaf nutrient status. Hop Propagation (PV) Hop cuttings represent a significant proportion of the cost of hop expansion. Some advances in techniques for providing large quantities of planting material from limited parent stock have emerged in recent years. The feasibility of these for possible rapid increases in acreage of Shinshuwase, J 78, B 23, or other varieties is planned. The most promising technique involves rooting leaves in large numbers in polythene growth chambers (without using mist). Leaves would be rooted in October and planted out to a nursery or permanent position in November. Carbohydrate Root Reserves (PV) Under natural conditions much of the stored carbohydrate in hop leaves and stems moves to the rootstock before the above ground part of the hop plant dies in autum. If the vines are harvested early in the season this cannot take place and it is generally acknowledged that early growth in the next year suffers becuase of inadequate reserves in the rootstock. It is intended to experiment with various defoliation procedures to ensure maximum leaf material on the basal portion of the vine not harvested. Tnis will involve modifying severity and time of defoliat ion of the lower leaves (which facilitates harvesting) and chemical tests to quantify carbohydrate reserves. Harvest Date Determination (PV,IE) Proper determination of the optimum time of harvest can contribute significantly to both yield and alpha content. For 1979/80, it is planned to intensify work in this area, with more intensive sampling and analysis, both using conventional tech niques, and by observing lupulin glands under a stereomicroscope. Spider Mite Control (PV,IE) The need for new outlooks on spider mite control is becoming more and more apparent because of increasing mite resistance to many of the mLticides (especially Tedion and Kelthane) and cost. The spider monitoring service will be extended, and evaluation of a synthetic pyrhethrum miticide will be- continued. Triploids (PV.TE) Trials will continue on the use of triploid male plants for increasing cone weight while maintaining near seedless condition. Shinshuwase Variety (PV, IE) Layered cuttings from the few plants grown in 1978/79 will be planted at both Bushy Park and Scottsdale for further bulking of material and yield-quality evaluation. Alternative Packaging (TE) A vacuum pack of pressed hops was introduced in 1978/79 with sales of 50 tonnes. It is proposed to further develop this pack by stream lining the production,, increasing the density, and properly document ing the storage alpha losses. Other packaging initiatives involve further selling of pellets in pouches without cartons, improvements in pack appearance with reductions in transit damage, possible press modifications to produce cylindrical bales for East German sales, and improvements in whole hop pellet production and quality. FOLIAR FERTILIZERS,REPORT ON THE 1978/79 TRIAL. ( J.P. Versluys ) July,1979. Summary. A 16# increase in dry yield was obtained after four applications of a complete foliar fertilizer on an area of hops which had been affected by hail in November. A similar trial which was undertaken the previous year gave an increase in dry hop yield of nearly °ff>. In both years,the absolute yield increase was approx. 100 Kg. of dried hops per acre. Based on Grower-returns for the 1979-Crop,the extra yield had a net value of $150 per acre,(after accounting for the cost of materials and application,as well as for expenses related to picking and drying). The efficiency of complete foliar fertilizers applied in addition to normal dressings of ground-applied fertilizers has now been proven 'beyond reasonable doubt1 and routine-use of these products on a broad-acre basis is recommended. Trial Layout. The material used was Wuxal (a Ciba Geigy-product),containing 9# N,4# P,6# K,and the trace elements B,Co,Cu,Fe,Mn,Mo,Ni,Zn. Treated area: bays No.3,4,5, J.Butler,No.1-paddock. Control: bays No.1,2, " " " All bays have 300 plants,area per bay approx. 1/3 acre. A total of H gallons/acre of Wuxal was applied,divided into four applications,each at the rate of 3 pts./acre in 130 gallons of water per acre. (4.2 litres in 1500 litres per hectare.) The application dates were 8/1,27/1,19/2,28/2. The first two applications were combined with Tedion/Kelthane miticides. Results. The t r i a l area was machi tie-picked on 16/3. W/D ra^io Kg.dry # LCV (dry Kg.green # D.M. Treatment,3 1018 4 5 1292 1113 21.3 20.3 22.0 4.32 4.53 4.18 235.6 285.2 266.3 15.3 15.4 13.3 1 800 958 22.7 21.7 4.05 4.24 197.5 2 225.9 14.5 14.7 Control, (*) calculated on 8# moisture after drying basis) The outer three rows of non-treated bay No.1,on the boundary of the paddock.were visibly affected by the prevailing westerly winds: in a comparison of treated and untreated areas,the results from bay No.1 have therefore been discarded. Average yield/plant,treated area,(900 plants) 0.874 kg.dry hops " <> « .control (300 plants) 0.753 kg.dry hops Discussion. The dry yield increase per plant was 0.121 Kg.,or 109 Kg./acre. At an average Grower-return of $2.10/Kg. in 1979,and at a marginally costed harvesting expense of 45 c/Kg. (20 c picking, 25 c drying),the extra return per acre was about $ 180. The cost of Wuxal in 1979 was $ 2.80 per litre.The quantity used in four applications was 6.8 litres at a cost of $19/acre. Two of the applications were put on at no extra cost in combination with miticide-runs.If the two additio«al Wuxal applications are costed at $5/acre each,the total cost of four applications would be approx. $30/acre. The extra net return per acre was $150. In a report describing the results of the 1977/78 foliar fertilizer trial (5/5/78),it was suggested that the percentage of extra yield after applications would increase under conditions of low yield in the non-treated areas. This situation happened to apply during the most recent trial: all of J.Butler's hops were severely affected by a hail storm in November: Yield per acre on Mr. Butler's property was 1075 Kg. in 1978,and 840 Kg. in 1979. It is interesting to note that the absolute increases in dry yield during the two years of foliar fertilizer applications were of the same magnitude: + 99 Kg./acre in 1978,and + 109 Kg./acre in 1979. Alpha-acids content was not affected by the treatment.' 10. SUBSTITUTION OF SOIL-APPLIED NITROGEN WITH FOLIAR SPRAYS. (J.P. VersluyB ) Summary. August,1979. The effect of complete foliar fertilizer solutions on hops which had received normal dressings of Nitrogen,Phosphorus,and Potassium has been described in previous reports (May 1978,July,1979). In an attempt to reduce the cost of soil-applied Nitrogen,a trial was undertaken during 1978/79 in which Poliverdol and Urea-solutions v/ere used to compensate for a decrease in Nitram applications fron 8 cwt./acre to 2 cwt./acre. Although yield in the low-Nitram plots was not depressed in any of the treatments,plants in the lowNitrogen areas showed all the signs of Nitrogen- deficiency despite application of 'compensating' foliar sprays. Reduced N-uptake in the low-Nitram areas was also demonstrated by means of foliar analysis.This further showed,that uptake of Potassium and Magnesium had been depressed by conditions of low soil-N. The trial again showed the beneficial effects of complete foliar fertilizers given in addition to normal levels of soil-applied fertilizers: a yield increase of nearly 180 kg. of dried hops per acre. Under similar conditions,applications of Urea-sprays only had a minimal effect.Trial Layout. ( H.D. Davey,'Kinegar-Top') (*) one of the sampling areas. X1,X2,X3 A = Control , „, ^ .. a , . ., = Nitram,8 cwt./acre + 3 applies. Poliverdol,total B = Nitram,2 cwt./acre + 3 I) » Nitram,2 cwt./acre + 1 8 pts./acre C " " " ", = Nitram,8 cwt./acre + 1 applic.Urea 10£ +2 appliCB.' Urea \1> " " + 2 " Each of the plots,X1,A,B,etc. is two bays (10 rows) wide and approx. 2/3 acre in area. All leaf samples were collected from the middle of each plot, as in *. 11. Nitram applications: all plots: 2 cwt./acre in mid-November X1,A,X2,C,X3:2 cwt. in mid-December,2 cwt. in mid-January,2 cwt. in mid-Febr. A,B : 18/12 (1i pts./acre),12/1 (3 pts.), ( Poliverdol: in 75 gals. 7/2 (3 pts.) ( ( C,D : 18/12 (10?S solution), 12/1 (1*), water/ ( Urea: 7/2 (1*). acre (N.B. An error was made in preparation of the first Urea-solution: this went scorching was done. rinsed in on at 10$ instead of 1$.Apart from some minimal on the leaf-edges of old leaves.no visible damage Leaf samples collected on 22/12 and 3/1 were water before oven-drying in order to wash off a possible Urea-residue.) Yield. On 30/3/79,60 plants from the sampling areas within each plot were machine-picked;samples were taken for analysis of Dry Matter $ and 1CT. The results are presented in the Table below,Plot Yield/plant, D.M. <f> W/D ratio (at 8^ moist) (green) Kg. Yield/pl. (dry) Kg. LCV (d.b. 1.221 13.6 3.64 1.299 13.1 3.50 1.114 14.2 11 4.53 24.8 3.71 A 4.73 25.3 B 3.90 26.3 X2 3.51 25.7 3.58 0.980 12.4 C 4.H 25.7 3.58 1.156 13.8 D 4.40 24.7 3.72 1.183 12.9 X3 4.65 25.0 3.68 1.264 14.6 At 7' x 7' spacing (890 plants/acre): Poliverdol effect* Id/acre Kg. Control (X1+X2) Normal Nitr.+ Poliverdol (A) 1156 low Nitram + Poliverdol (B) 991 (Treatment Control) 979 + 177 Kg. + 12 1029 + 30 1053 + 54 Urea effect: Control (X2+X3) Normal Nitr.+ Urea (C) low Nitram + Urea (D) 999 12. Leaf Analysis. Leaf samples consisting of the first two pairs of fully-spread leaves below the growing tip of main vine or lateral were collected from each plot on 7/12,22/12,3/1,1/2,16/2,15/3; 15-20 plants per sample.Only the leaf tissue was dried and analysed,petioles were discarded. The results are given in Figures 1-8. Discussion. None of the treatments had a negative effect on hop yield.The yield-'increase' of 12 Kg. after Poliverdol applications on the ^J low-N plot is probably not significant. Yield increases after Urea applications were small too,and rather odd in that the low soil-N plot had a higher yield than the plot which had received normal Nitram. If real,this difference cannot be readily explained. (The results of a trial at the Tobacco Research Station in Myrtleford in 1977/78 gave a similar,negative, ' response to increasing rates of soil-applied Nitrogen.) The decrease in leaf-Nitrogen content is immediately obvious (Figures 1,2) in the low-Nitram plots despite application of Poliverdol and,to a lesser extent,of Urea solutions.The quantities of Nitrogen applied as Poliverdol and Urea were very small (0.4 Kg . N in 1 gallon Poliverdol,and approx. 19 Kg. N in the three Ureasprays) in comparison with a reduction in soil-applied Nitrogen from 138 Kg.N/acre to 34 Kg.N/acre and in relation to a total removal of 50-60 Kg. Nitrogen per acre by cones,vines,leaves. Phosphorus contents of the leaves were not affected by any of the treatments (Figures 3,4),but both leaf-Potassium and leaf-Hagnesiui were reduced in the low-Nitram plots (Figures 5-8). ! Despite the fact that yield reductions did not occur in the lowNitram plots,the overall effect of Nitrogen fertilization on the uptake of other elements is clearly demonstrated. The possibility of a carry-over effect of low soil-Nitrogen on the next crop will be investigated by recording the 1980-yield of the same areas next year.' Conclusion. 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M \ m_.. •Mi — \ — — Mi ' l i : on i tijmij. ii mi ii iii - | i. MM I 1 I i.i I , t i * * Ml: . I . > Mi . M ! o 21. HORMONE APPLICATIONS,REPORT ON THE 1978/79 TRIALS. ( J.P. Versluys ) July,1979. Summary. Foliar sprays containing the hormones Gibberellic Acid and 2-Naphthoxyacetic Acid were applied on three trial sites immediately after burr. On each of these sites comparisons were made between applications with tractor-drawn equipment at 200 gals./ acre and 100 gals./acre and application by aeroplane at the rate of 10 gals./acre.The quantity of active ingredients per acre was the same in each case. The effects of these applications were: 1.0n two of the sites,ripening in comparison with non- treated hops was delayed by 8-10 days after applications of 200 gals./acre and 100 gals./acre.It is demonstrated that there is a chance of less than 1: 100 of these delays having arisen by chance. 2.Aerial application at the rate of 10 gals./acre did not delay ripening on any of the sites. 3.Non-treated controls and hops treated with high volumes of hormone solution were machine-harvested on two of the sites.Yield increases in the hormone-treated areas, calculated as dry product,varied between 30 - 200 Kg./ nfjr'g 4.Alpha-acids content was not affected by any of the treatments* Recommendations. I.The significant delay of ripening that occurs when hops are treated with extremely dilute solutions of Gibberellic Acid and 2-Naphthoxyacetic Acid has now been demonstrated for the second year in succession (ref. 'Hormone Applications to Increase Yield', 4/5/78).Delay-periods were of the order of 5 days in 1978 and 9 days in 1979.The practical application of this is in extending the harvest period .particularly in Victoria. In Southern Tasmania and in the Scottsdale area the advantage of extending the picking season will have to be weighed against the risk of nightfrosts during the tail end of the harvest period.lt is proposed to carry out a series of commercial-scale applications at various properties in Victoria during the 1979/80 season. 2.Yield increases as a result of hormone treatments varied considerably between the various trial sites in 1979.Further verification of this effect is needed and additional trial work should be undertaken during the next season.The effect of these hormones on the rate of resin gland filling would also be studied. 22. Trial Layout. 5 grammes 'Berelix' Treatments: ^',200 gals./acre ) ( 90 %GA5) /acre 'B',100 gals./acre < 0.36 grammes 2-Naphthoxy »C, 10 gals./acre ) acetic Acid /acre Sites: Bowen.Scottsdale,approx. 240 olants/treatment Bartlett,Legerwood, n Edwards,Branxholm, " 160 340 " " " " Treatments 'A» and •B« on 14/2/79 Treatment •_• on 17/2/79. Application dates: Jcb Plan: schematically presented below.In case of treatmen. A and B the two middle rows of each bay were sprayed.The swath width of the aerial application in aays 'C* was approx. 30 ft. 11,2,3,4 are Control areas. | z A 1 X2 B 13 C 14 1 bay Sampling. Details of Dry Hatter Contents of hand-picked samples are presented in Tables I - III.Each sample consisted of all cones picked from 6 laterals which were collected at random from the two middle rows of each treatment and each control at a height of approx. 14 ft. The relation between date of sampling (= Day No.,x) and Dry Matter % (y) was calculated from Tables I - III as follows: 23. cocff.of correlation Bowen (N = 7) Control: A B C 8.49 < 0.01 7.88 <0.01 y = 0.29x + 14.8 y = 0.49x + 12.5 0.874 4.02 <0.01 9.25 <0.01 0.972 0.709 7.25 <0.01 5.02 <0.01 5.72 <0.01 Not - lign. 0.895 0.905 0.767 0.790 4.49 5.18 2.67 2.88 Bartlett Control y = 0.21x + 16.0 0.964 (N = 6) A y = 0.22x + 19.6 B y = 0.20x + 14.2 0.929 0.944 C Edwards (N = 7) Control A P t 0.967 0.962 y = 0.34x + 14.7 y = 0.42x + 13.7 y = 0.18x + 15.8 y = 0.20x + 13.9 B y = 0.18x + 15.4 C y = 0.16x + 16.9 <0.01 <0.01 <0.05 <.0.05 Delay-effect. Statistically-significant functions between Time and Dry Matter % have been drawn in Graphs I-III. From Graphs II and III it may be seen that maturity has been delayed by approx. 10 days for treatments A and B in the Bartletttrial,and by approx. 8 days for treatment A in the Edwards-trial. It is probable that hormone application on 14/2 in the Bowentrial was too late to have an effect: The Control area at Bowen's had a DM content of 21.5# on 13/3,that is 27 days after the application took place.In the Bartlett-trial it took until 19/3 for a DM content of 21.5# to be realized in the ncn-sprayed area, and in the Edwards trial that did not occur until 24/3: respective ly 33 days and 38 days after hormone application, -.achine-.ickine: and Yield.. The Bartlett-trial was machine-harvested on 26/3 with the following results: Control' 161 plants from Controls X1 and X3, green: 6.478 Kg,/plant LCV (dry m content on day 33(via y=0.21x + 16.0) = 22.93#, basis): 12»° A: i.e. wet/dry ratio at 8£ moisture 4.012 dry: 1.615 Kg./pi. 72 plants from treatment A LCV 13.5 B: LCV 12.3 77 plants from treatment B green: 6.597 Kg./pi. w/d dry: 4.012 1.644 Kg./pi. green: 6.753 Kg./pi. w/d dry: 4.012 1.683 Kg./pi. N.B. the influence of the delay-effect on the calculation of dry product from treatments A and B has been negated by using the wet/dry ratio of hops from the control area for conversion of green product from A and B, 24. The Edwards-trial was machine-harvested on 29/3 (Day No.36). Control: 680 plants from Controls X2 and X3, green:7.356 Kg./pi. LCV 11.3 DM content on day 36 (via y=0.18x + 15.8) = 22.28#, i.e. wet/dry ratio at 8% moisture dry: A: 340 plants from treatment A green:8.326 Kg./pl. w/d LCV 10 dry: B: 340 plants from treatment B 4.129 2.016 Kg./pl. green:7.503 Kg./pl, w/d LCV 11.5 dry: Bartlett: Edwards: 4.129 1.187 Kg./pl. Control 1.615 Kg./plant,dry A B 1.644 1.683 Control 1.782 A 2.016 B 1.817 4.129 1,782 Kg./pi. + 1.8#,or + + 4.2#,or + 26 Kg./aci 60 Kg./ac. +13.1#»or + 208 Kg./ac. + 2.0/o, or + 31 Kg./ac. 25 D.Vt. Contents of hand-picked samples. Trtblo I. Date Day No. Control,Average A S BOWEN. 0 (bays X1-X4) 23/2 27/2 1/3 5/3 7/3 9/3 13/3 2 15.7* 6 8 16.9 12 18.4 14 16 20.3 20 16.9 19.4 22.0 13.6 17.6 16.8 14.4 18.0 19.3 20.0 20.0 22.0 19.3 19.3 19.0 20.0 16.0 BARTLETT Table II. 22/2 28/2 6/3 8/3 12/3 26/3 7 13 16.1 16.4 16.9 15 19 33 17.8 18.8 22.4 1 15.3 14.0 16.0 17.0 . 17.3 21.7 15.3 15.6 15.7 16.7 18.7 21.3 1 5 7 13 15 23 30 16.3 16.4 17.7 16.3 19.7 N.A. ED'JARDS. Table III. 22/2 26/2 28/2 6/3 8/3 16/3 23/3 14.0 16.0 15.2 18.7 19.7 19.7 23.0 16.1 16.8 18.2 17.6 16.8 20.4 21.6 15.3 14.0 16,0 15.7 15.7 18.3 20,7 15.3 16.3 18.8 18.8 14.8 18.2 19.2 18.7 17.0 21.3 22.0 16.3 20.7 20.7 . !_!__ j 1 U i 1 I 1 i i. M i — --L. ; _ _ . . i. „ . ... ,i . . i. . .:...i. .._._ .:..__ -HOR l.OMT! APPLICATION-TRTAT.. 1Q7C- * d.i: 24 • 1 ! I ^Relationship J- - J_HJY.i__. •iii' ( x)__;DRY iMfi - 23 1 TIME ^ 1^ 1 •" ..:. MTTER j _(y I • i• i 1 :.:: :.:: : ! ::• .:., • • ! 1 . _ j _' _ _ — 22 - •: , : ::: ' .—•.>. ^^ 21 20 ::.: . 1 ':;: __B: ^ ...... - - CONTRO —> •- ; ]': i il A :!:: ::;- . '• ' 19 • :: r: ^ ...: 18 • \v-y ^M_ ^-— S^ «*Mf '•. : ./ 17 - i" ! —— ;S 16 " 1 14 • t • i i '.::: —~- />::: iiii > 15 • • - •'•'*' -1 y :: . .:: ii :::. A1 'iii :::.! . : i: ' !: .... "~Z ::: :- .-;-:: . *. * ' :!: • '. r .; L-\\ 1r :::: 13 - :.; i . _ _ -—'• — '; ••:'•/: i Date Day No, 22/2 '. '.,'' ..... i ::: i . 1 i > 1/3 :::: - ii ::.: • • < 1 •—T- i -—:: i i ! i '• • * t • 1! :>.o • i:: ::; • 25 '. I i i 10/3 Mii|oi i: _—i. 1— :; \"\- • 4 :::: ~i I ' ! •I BARTLETT (II) ;HORMONE APPLICATION TRIAL,1979. * D.M '•-•:•: j• • j '• i : i Relationship. TIME. (x) _ DRY MATTER, jt (y) Date Day No Date Day No. OO _y. 29 REPORT ON ICSCELLANEOUS EXPERIMENTS BUSHY PARK HOP NURSERY - 1979. Shinshuwase Variety Low Trellis Alpha Acid Variation Triploids Daylength Extension Acute Angle Trellis Spider Mite Monitoring I.J. EDWARDS. MAY 1979. _u. CONTENTS PAGE SUMMARY SHINSHUWASE VARIETY 1st year harvest indicates that this variety yields quite well with about 5% alpha. Material is being prop agated for possible Japanese market. LOW TRELLIS Very promising with yield up to 860 kg./acre (2nd year plants) but iiodified picking needed to realize its full potential. FACTORS AFFECTING Most chemicals applied as a foliar ALPHA spray decreased alpha but one slightly increased it. High alpha varieties located in 1978 did not differ markedly from Pride of Ringwxxl in 1979 after transplanting to the nursery site. ACUTE ANGLE GROWTH Vines trained up string (normal 16' height) on angle of 60° did not 'differ in yield or alpha frcm a control at normal string angle. TRIPLOIDS Trial en use of triploid male hops for increasing cone weight and lower ing seed content failed due to wither ing of hop flowers in special pollinat ing bags. ARTIFICIAL DAY LENGTH EXTENSION Artificial light delayed flowering by 2-3 weeks, but paddock trellis (10 acre farm area) fell down before completion of tests. SPIDER METE M0OTTORING The mite counting service offers a sensible guide to mite control while minimising unnecessary spray use and resistance problems. 4. 31. 1 - SHINSHUWASE HOPS Eight (8) cuttings of Shinshuwase hops, recently released from Plant Quarantine, were planted in a nursery bed at Bushy Park in August 1978. The hops were grcran under noncultivation conditions, with regular fertilizer applications. They were treated the same as adjacent "Pride of Ringwood" and other hop varieties in the nursery. Most of the plants were layered when they reach trellis height (6 feet) as the main aim was to bulk up material for propogating. Layering prevented proper yield determinations but in general the plants were vigorious and compared favourably with adjacent first year "Pride of Ringwood" hops. It may be noted that the period of maturity is almost identical with Pride of Ringwood that the cones are quite heavy and that the alpha acid averages about 5%. No analyses were made on essential oils, beta and hard resins or storage stability. SHINSHUWASE DATE ALPHA ,£>RY MATTER FIRST YEAR PRIDE OF RPram) CONE WT. ALPHA 7cDRY MATTER CONE WT, (nig) (tig) 7.0 8/3/'79 3.0 14/3/'79 5.0 21.3 132 9.0 21.3 203 20/3/'79 4.1 23.5 149 7.5 24.6 205 27/3/'79 5.0 24.8 136 9.5 24.9 179 2/4/'79 5.0 25.8 125 9.0 26.4 211 72. 32. LOW TRET ITS 1979 represented the second year of a low trellis trial. Pride of Ringwood and Golden Cluster hops were trained rp trellis varying from 5'6" to 7' in height. String angle was 60° frcm horizontal, string spacing 24" and rcw spacing 7'. Vines per string averaged 4-5. Second year plants were not cultivated at all, fertilizer was applied at 7 cwt/acre. Basic P/K mix in August, and 6 cwt anrnonium nitrate through the growing season. Cones were harvested by hand from representative 10' sections, and converted to dry weight per acre. Results are presented below:Yield Alpha* (Kg./Acre) 1st yr. P. of R. From standard cuttings, at close spacing (6"). 1st yr. Fran 1 year old rooted plants P. of R. transplanted intact. 2nd yr. 80" trellis height, 60° P. of R. angle. 2nd yr. 66" trellis height, 60° P. of R. angle. 2nd yr. 80" trellis height, 60° Cluster angle. Average yield mature Pride of Ringwood at Coniston (85 acres) 260 9.0 274 9.5 864 12.1 720 13.0 760 8.0 1,243 10.0 * Alpha based on single determination - "as is" polarimetric alpha after drying. Cuinitemts Seccnd year plants yielded exceptionally well, with large cones, distributed virtually to ground level. Because of the dense, interlocking nature of the vines, the interior suffered from lack of light, and the few cones formed here were somewhat chlorotic, of 2-37, less dry matter, and 1-27, less alpha. The vines would pose picking problems as grown this way because of interlocking of vines, but this could be overcome by extra wires. A modified picking technique with this type of trellis would offer significant advantages: eg. a self training system where new vines grow up the raiiaining dead vines from the previous crop; where no costs are incurred for string, stringing, or training (normally $200/acre); and where .../3. -._. - 3 - harvesting takes place in situ, with leaves and cones blown (or possibly sucked) off the vines with a simple mobile harvester. Leaves would subsequently be separated from cones at existing stationary pidcing machines. The low trellis shows considerable promise and trials will continue in 1979 with emphasis on systems for practical harvesting. 74. 34. - 4 ALPHA ACID TRIALS 1. Effect of Foliar Sprays on Alpha Acid A trial was conducted at Bushy Park in 1979 to indicate the effect of foliar applied chemicals on alpha. Chemicals were applied with a knapsack spray just as the plants were going from burr into hop. Three different concentrations were applied, varying from maximum concentration of the chemical capable of passing into solution to 107, of this maximum level. Alpha readings were taken from all vines not killed by these concentrations. Average data is presented below. Alpha Chemical No. of Samples 11.8 12.3 10.5 11.8 11.6 10.5 Control KH2P04 Potash H3B03 Urea CaO *feS04 MgO 7.3 FeS03 MnS04 9.9 8.6 4 5 1 2 2 1 1 1 1 1 8.9 Although the concentrations of chemicals used in this trial were rather high, the effect of many of the chemicals on alpha was dramatic. The increase with KH2P04 was non significant but provides an indication that it may be possible to increase alpha (as well as yield) with certain chemicals supplied as foliar sprays at critical periods. 2. Chance Location of High Alpha Wild Hops Another experiment indicating a significant effect of soil conditions on alpha resulted from trials on high alpha wild hops located in 1978. As a result of over 200 alpha deter minations on wild hops that had grown from seed among normal Pride of Ringwood, up fences and blackberries etc., 6 cultivars were selected for propagation in the winter of 1978 and were analysed for alpha acid in 1979. The results are presented below :- Cultivar Control I.G. 14/4/7 12/4/29 14/4/3 14/4/11 31/3/26 P of R 1978 Alpha 10.0 Location: 1979 Alpha Location: 9.8 15.5 15.2 14.5 14.9 14.8 All growing up fences and stay wires NA 6.9' 9.7 9.8 All planted in nursery bed ...15. 7.9 14.5 10.3 35. 5 - In this trial all the cuttings propogated in the nursery garden gave much lcwer alpha when transplanted to the nursery site, probably indicating that the reason for the high alpha in 1978 but not 1979 was attributable to soil nutrients or lack of cultivation. It is also possible that not picking the hops in one year increases the alpha in the subsequent year. .../6. 36. - 6 - TRIPLOIDS Pollen was collected from male, triploid hop cultivars transplanted frcm the C.U.B. nursery in Ringwood to Bushy Park. Pollen was introduced to female Pride of Ringwood hops during flowering with wild pollination prevented by special pollinating bags designed to let air and light, but not pollen, penetrate. The triploid hops were to be tested for ability to increase cone weight while naintaining hops in seedless condition (less than 27, seed). Unfortunately this experiment did not work because the Pride of Ringwood hop flowers in the pollinating bags withered and eventually died. It may be noted that Graham Hughes experienced the same problem at Ringwood in a similar trial. The cause of the adverse environmental conditions within the pollinating bags is unclear. The bags apparently work satisfactorily in England, frcm where they originate. More trials are proposed for next year. A recent publication (Crop Science Vol. 19, January February 1979 p.29-31) presents evidence that yield increases of 15-2570 can occur using triploids, with4-57. seed content. This paper is based on American experience and does not automatically relate to likely Australian conditions with Pride of Ringwood hops. ...II. 37 ACUTE ANGLE TRELLIS Research at Wye College in England showed that yields of many English varieties were maximised when the string angle was 60° frcm horizontal. A trial was run at Bushy Park in corporating one small bay of the paddock. Bushes Comer, in the Bungalow area. In practice, strings were left attached to the top wires in the normal positions, and moved across one hill at the base. The string angles were 67° (2/3 strings) and 58° (1/3 strings). The direction of the inclination was alternated each rcw. Tractors could still move along rows for cultivating, but not across rows. The area was treated as normal for irrigation, fertilizer, cultivation etc., and yield and alpha determined at harvest by pidcing 10 strings at random in both the treated and adjacent control areas. Results: Yield and alpha in both the sites were almost identical. Ccntrol - 686 Kg/acre, alpha 10.2 Oblique - 713 Kg/acre, Angle alpha 10.3 Results are not significantly different. Canments: The site was late trained, and generally low in yield. It is more likely that the oblique angle trial would have shown an advantage if the yield had been higher,since light interception by leaves is better in the oblique arrangement. 38 - 8 - DAY LENGTH EXTENSION TRIALS Hops are so acutely sensitive to day length that flcwering date is almost totally independent of other climatic and cultural factors. The light intensity required for the photo periodic response is quite low, and artificial day length extension is practised cocnnercially on many plants, especially ornamental flowers. A trial was set up at Bushy Park to test the feasibility of extending the hop harvest season by delaying the time of flowering. A mercury vapour lamp (400 w) was installed just inside a window in the Hansens Kiln beside the paddock "10 acre" in the "Farm" area. The light illuminated about l/20th of an acre sufficiently to inhibit flowering. The light was left running 24 hours per day frcm the 20th December, 1978 to the 10th February, 1979. Results: Flowering was delayed to a degree depending on the proximity of hop plants to the light. Those adjacent to the light flowered approximately 3-4 weeks after a control. Those further away flowered unevenly with some flowers at the normal time, and some up to 2-3 weeks later. Unfortunately this was the one paddock in the whole Bushy Park hop complex which collapsed in a thunderstorm, and the experiment was abruptly terminated before relevant maturity dates and alpha acid contents could be established. 79. 39 SPIDER MITE M3NIT0RING The 2-spotted mite or Red Spider Mite is the only serious pest attacking the hop plant. Seme growers spray for mites 2-3 times through the growing season, while other growers do not spray at all. Damage varies considerably from year to year but is generally worse in hot weather, especially in Victoria when the mite populations can rapidly" (2-3 weeks) increase to plague proportions. Damage is expressed through unsightly hop cones, reduced yield, and often the need to start harvesting when hops are inmature. A routine monitoring service was established by A.H.M. in the 1978-'79 season. Growers were invited to forward 50 leaves per paddock every 2 weeks. The leaves were soaked in a solution of hot (65°C) water, sodium hypochlorite (27,) and sodium lauryl sulphate (5 drops) for at least % an hour, then shaken and the solution filtered to 200 microns. Mites were counted in water in a petri dish (with a black grid for background) using a stereomicroscope. Growers were advised of average count per leaf and spraying reexxirnendations witMn 3 days of collecting leaves. Cctrments: Not all growers contributed leaves, and not all those who did use the service followed the reconmended spraying procedure. Generally speaking those growers who traditionally sprayed 3 times per year used the service primarily to determine sequence of paddock spraying and were inclined to spray when the count indicated spraying was unnecessary. (Bushy Park Estates, Panlook and H. Davey). Other growers, concerned at possible spider infestations but with no firm opinions, used the service as reaxrinended with great success (Keir, some Scottsdale growers). Many growers not traditionally worried by spider mites did not submit samples. It is noteworthy that 1979 was a particularly bad year for the spider mite. Some growers who did not use the service, (Graham, Frankcomb) had serious spider problems which necessitated premature harvesting and some yield losses (Data on effect on yield from L. Miller, Department of Agriculture, trials). On the other hand, regular users of the service had no serious problems, often despite bad outbreaks among neighbours (especially in Victoria). .../10. 40 10 - In conclusion, the mite monitoring service worked well with grower acceptance gradually increasing. The savings are hard to quantify but are possibly as follows:- Bushy Park Estates - saving in miticide $10,000 c.f. normal spraying programme Other Growers - saving in miticide $10,000 Value of increased yield in Victoria c.f. mite damage which would almost certainly have occurred (27, @ $2-10 kg. on 240 Tonnes) $10,000 The service cost approximately $1,000 for analysis Mite (especially Kelthane -Agricultural Department data) and resistance to seme of the comnon miticides is increasing objective mite counting would seem to offer a sensible guide to mite control while minimising unnecessary spray use and resistance problems. 41. ANTIOXIDANTS. MATERIALS AND TRIALS. ( J.P. Versluys ) Summary. September,1979. Addition of 0.4 Kg. ascorbic acid per 1 Tonne of hop powder prior to pelleting completely halted alpha-acids deterioration in pellets kept at room temperature for 4 weeks. During that time,untreated pelTets lost alpha-acids at the rate of aoprox. 1% (relative) per week. At 30° C.,the rate of alpha-deterioration was slowed down progressively with increasing quantities of added ascorbic acid,to a point where the deterioration in pellets which had received 4 Kg. ascorbic acid per tonne was 5 x slower than in untreated material. The potential savings obtained by AA-additions may be calculated at 8200/Tonne pellets at 1979-prices. Attempts to increase the rate of synthesis of ascorbic acid within the hop plant by foliar applications of sugar solutions were not successful. Introduction. The application of antioxidants in the food processing industry is as materials which are so readily oxidized,that a preferential oxidation of these materials takes place.Given a product storage-environment in which only a limited quantity of oxygen is available to sustain an oxidative process (as in vacuumpacked hop pellets),an antloxidant/product ratio may be determined that will alow complete oxidation of the additive whilst leaving the product intact. The range of 'natural* antioxidant materials is limited and the two main,plant-produced,compounds are tocopherols (e.g. in vegetable oils) and ascorbic acid (vitamin C,in fruits,etc.). American work in 1949 (1) showed that the antibiotic properties of hop resins could be prolonged by additions of ascorbic acid (AA). In 1977,Russian workers demonstrated that tocopherols (2) as well as AA (3) were being synthesized by hops. The tocopherols are found in the lupulin glands in concentrations of 200-700 p.p.m. Alpha-tocopherols (vitamin E) account for 100-550 p.p.m.;these are inactive as far as the alpha-acids are concerned.The 'rest' of the tocopherols (20-300 p.p.m..depending on hop variety) increase the stability of the alpha-acids. AA-concentrations in fresh cones after drying were within the range of 25C-350 p.p.m. (compare vitamin C content of apples, 300-500 p.p.m.;le_ons,500 p.p.m.;rosehips,2300 p.p.m.). 42. The obvious attractions of using AA in pelleted hops were that it is a low-cost material (approx.$10/Kg.) when used in extremely low dosages,and the fact that AA is being used by some breweries as an antioxidant material,(4,5). 1.Analysis. During October,1978,some preliminary analyses were carried out on Pride of Ringwood bales and pellets held in A.H.M.-coolstores. The presence of AA in this material was confirmed: 1975-bales 60-80 p.p.m. AA 1977-pellets 380 p.p.m. 1978-bales 330 p.p.B. 1978-pellets 560 p.p.m. 2.Pelleting Trial. 2.1.Experimental. During the first week of November,1978,a pelleting trial was undertaken at Bushy Park,using bales of the 1978-Crop. The following treatments were carried out: AA-addition to ground hops AA,found by analysis of pellets (a) (b) (c) d) fe) (f) Nil (control) 400 800 4000 400 800 p.p.m. p.p.m. p.p.m. p.p.m. p.p.m. (powder) (powder) (powder) (in meth.spir.sol) (in meth.spir.sol) 330 p.p.m. 790 610 3200 710 660 A comparison between AA levels found in the finished pellets and calculated AA levels (after addition of known quantities) showed that an average of 25$ of added AA had been oxidized during pelleting, and regardless of whether the addition had been in powder-form or as a solution in methylated spirits. Pellets were vacuum-packed in oxygen-barrier sample pouches. Evacuation was (i) to better than 99$ of absolute vacuum,and (ii) to 80# of absolute vacuum (0.8 Vac).Sample pouches were stored at 30° C. (± 1°) and at room temperature. 2.2.Results. Analysis of LCV after 4 weeks and 40 weeks showed that the . oxidation of alpha-acids in samples kept at 30° C. was 3-4 x greater than in samples kept at room temperature.(A similar 43. difference in deterioration rates of AA was found in samples kept at room temperature and at 30° C.) At both temperatures,the oxidation of alpha-acids in samples packaged at 0,8 Vac. was about 20$ greater after 4 weeks than the alpha-oxidation in samples packaged at full Vac. After 40 weeks,the total alpha-oxidation was much the same at both levels of vacuum. (By contrast,the rate of AA-oxidation in samples packaged at 0.8 Vac. was twice that (20096 greater) than in samples packaged at full Vac.) Addition of AA-solutions had no effect at all on the rate of deterioration of alpha-acids in any of the vacuum/temperature combinations. (room-T.) During the first 4 weeks of pellet storage at room temperature, all additions of AA in powder form completely halted oxidation of alpha-acids.During that time there was a relative loss of alpha-acids of 4-5% After 40 weeks,none a measurable effect loss after 40 weeks in of on at the control-samples. the AA powder-additions any longer had alpha-acids deterioration, (Alpha-acids room temperature was approx. 12% in all treatments and controls.) (30° C.) After 4 weeks at 30° C.,the average alpha-acids content in samples packaged at full Vac. and treated with AA was only marginally higher than alpha % of the control. After 4 weeks at 30° C. and o.8 Vac,the relative alpha-deterior ation was as follows: AA,+ 400 p.p.m. -6.6% -4.8% + 800 p.p.m. +4000 p.p.m. -2.4% -1.2% control After 40 weeks at 30° C.,none of the AA/vacuum combinations any longer had an effect on alpha-deterioration;the average relative loss after 40 weeks was 34%. 2.3.Discussion. In the present trial,indications were that alpha-acids deterioration in vacuum-packed pellets stored at room temperature could be arrested completely during the first four weeks after pelleting by addition of 400 p.p.m. AA to the hop powder prior to pelleting.During these first four weeks,the relative deterioration of alpha-acids in untreated pellets was approx. 1% per week. (i.e. very much the same rate of loss as calculated from reports of pellet-alpha on receipt by overseas and domestic clients). 44. It is not known for how long after pelleting the 'AA-effect' continues to function;the effect was no longer noticeable after 40 weeks. Neither is it known yet,if there is a difference between the AA-effect on pellets made at time of harvest from fresh hops, and the effect on pellets produced from bales which have been subject to an initial deterioration: hops used in the present trial were 7 months old when pelleted. On a production scale,it may prove extremely difficult to uniformly mix less than 0.5 Kg. powder in 1000 Kg, ground hops/ If pellet deterioration can be halted for 8 weeks after harvest by addition of 0,4 Kg. AA per 1 Tonne ground hops (at a materialscost of $4/Tonne),the benefits on export alpha-transactions would be approx. $200 per Tonne at 1979-price levels. 3.Synthesis . Since ascorbic acid is an endogenous hop constituent,it seemed a logical step to try to increase the rate of AA-synthesis within the plant. Several sugars act as precursors of AA,and some sugars can be assimilated by plants through the leaf tissue. During February,1979,two hop plants were knapsack-sprayed to run-off with 10$ sucrose-solutions: (A) applications on 7/2 and 16/2, (B) application on 16/2. AA-determinations on hand-picked cones (15/3) gave the following results: (dry matter basis) Control: 362 p.p.m.}(A),382 p.p.m.; (B),381 p.p.m. 4.Further Work. Trial work on the effects of addition of AA and the antioxidant material Santoquin is continuing.Further work on the effect of foliar applications of various sugars on AA-synthesis will be undertaken during 1980. References. 1,¥ichener,H.D. * Andersen,A.A.,Science,1949,110:68-69. 2.Ezhov,I.S. ft Guretskaya,V.F.,Ferment.Spirt.Promysh.,1977:17. 3.Lyashenko,N.I.,Priklad.Biokh.Mikrobiol.,1977,13:118. 4.Postel,W.,Brauwissenschaft,1972,25:196. 5.Baetsle,G.,Fermentatio,1974:83. MO. 45 VACUUM PACKED PRESSED HOPS 1979 REPORT BY: I.J. Edwards, May, 1979. 46. SUMMARY: Production No. Cartons 47873 Kg. 1105 Av. Weight 43.3 Kg. Pressed hops were vacuum packed satisfactorily with no serious problems. Initial labour costs were high (lOc/Kg Labour) but were reduced to approx. 4o/Kg toward the end due to streamlining of the individual packing oper ations. Average pack weight was low at 43.2 Kg. due to unsatisfactory pressing of the high density bales by most growers. (Exception Bradshaw at 48.8Kg.) This low weight added 1.9c/Kg. to total costs (cf 50Kg pack) and 4.0c/Kg. to overseas freight cost. Assuming customer reaction to this pack to be satisfactory, it is proposed that one high density press be upgraded for 1980 to produce bales exclu sively for vacuum packing at about 500 Kg/M3 density at 65 Kg. weight (cf 330 Kg/M3 or 43 Kg in 1979). This will reduce unit processing costs by 4.5c/Kg, and freight costs by 9.8c/Kg. The vacuum pack, even at only 43Kg. per cartons, provides the cheapest means of exporting alpha acid from Australia to N. America or Europe. INTRODUCTION: Vacuum packing pressed hops involves the following operations:- 1. High Density bales are stripped of their polypropylene pack and cut into two 17." long mini bales - excess material is pelleted. 2. A pouch is inserted over the half bale and a carton over the pouch. 3. The pack with pouch and carton is inverted onto a roller conveyor and moved to the normal vacuum sealer fitted with a 44" long sealing bar. ing. The pouch is heat sealed to within _" of the end before evacuat Air is then drawn out of the bag using a flexible suction tube fitted with a thin metal tube at the end for insertion in the small gap between the end of the bag and the sealed section. Air is extracted to 20" Mercury vacuum; the tube is then withdrawn and the corner of the bag heat sealed over the first seam and the side seam. 4. Handles are fitted by passing nylon strapping through slots in the carton flaps, and through holes in small pieces of 3mm plywood inside the flaps. The strapping is stapled behind the plywood thereby providing very strong handles each side fo the carton which cannot slide or move from side to side. 5. The flaps are closed, then taped and the pack weighed and identified with source H.D. Bale. The packs are strapped on pallets 4 high X 4 per layer = 16 cartons = 688 Kg/pallet. 47. PACK DENSITY: Density of High Density bales varies considerably, and it is not convenient to have each vacuum pack identical in weight. Improve ments could be made in this direction by more care in pressing the High Density bales. Average pack weight was only 43.2Kg, and sig nificantly increased cost (by 1.9c/Kg + 4.0c freight) over the 50Kg possible in properly pressed bales. Density in properly constructed high density bales is about 380 -400 Kg/M3. This is a limit imposed by:- (a) pressing pressure in readily available hydraulic presses. (b) lupulin gland rupture caused by high pressure. In normal High Density bales, the lupulin gland damage is already considerable, and is the basic reason that some customers (especially Guinness) have rejected the bales despite freight savings, and despite the lack of documented evidence of excessive alpha acid storage losses. Vacuum packing hops from High Density bales dramatically lessens the rate of alpha degradation, and.will presumably mean that customers will accept pressed hops of much greater densities. The success of vacuum packing for pellets at over 600Kg/M3 with complete lupulin gland rupture requiring only ambient temperature storage and freight attests to the lack of cause for concern with some lupulin gland damage under vacuum storage. The problems associated with construction of presses to compress hops to more than 400Kg/M3 have not been properly costed. Fred Peacocks' estimate of the conversion cost ofaHigh Density press to produce 135 Kg bales (for two 65Kg vacuum packs) is approximately $5,000 (= 2.5c/Kg. at 25% Int + Depn for 50 tonnes). The benefits of increasing density would be substantial as tabulated below:* WEIGHT ** COST OVERSEAS C/Kg FT. COST TOTAL COST (PACK.+ FREIGHT) 43 Kg. 13.7 29.0 C/Kg 42.7 c/Kg 50 11.8 25.0 36.8 65 9.1 19.2 28.3 Powder Pellets. 25 27.7 17.0 44.7 Whole Hop Pellets. 20 25.9 21.0 46.9 Current Vac. Packs, * Based on actual processing costs, 1979, (not selling price). ** General cargo costs to Europe or N. America. It may be noted that even at current densities the vaccuum pack of pressed hops represents the most economical means of delivery alpha acid to the Northern Hemisphere. The cost would be reduced a further 14.4c/Kg. by increasing density to provide a 65Kg pack. 48. PRACTICAL ASPECTS OF VACUUM PACKING: The longer sealing bar purchased for these packs to fit the existing vacuum heat sealer worked satisfactorily, but difficulty was experienced in preventing the entry of air between pouch and bar. The long sealing lengths and rigid pouch material posed problems due to crinkling, with resulting physical difficulty in obtaining the required vacuum>and poor sealing. After some trial and error the method outlined in the introduction whereby air was withdrawn with a tube after initial sealing to within half an inch of the end of the pouch was considered most practical. Handles were initially attached by strapping a nylon band around the packs and crimping a loop on each side to act as a handle. These were slow to attach, and awkward to carry because the surrounding strap would move laterally when lifted i.e., it was difficult to keep the handles exactly in the middle of the pack for correct balance. The method was subsequently modified as outlined in the introduction. This technique works very well, offering a practical combination of strength, convenience and simplicity. GENERAL COMMENTS: Vacuum packing pressed hops is technically quite simple. Although the system appears cumbersome because high density bales have to be first pressed and then broken down, trimmed and vacuum packed, in practice it is hard to envisage a more economical system. The basic problems with vacuum packing in a single operation at pressing are that:- (a) The hops are spongy in nature, and would require vacuum packing while under pressure . Even then the bags might subsequently burst, especially the few which develop slow leaks. (b) The pouch material is readily ruptured and could not stand up to the abrasion that a pack is subject to in the chamber of a hop press. (c) Expensive vacuum sealers would be required in each kiln. Some improvements might be possible though, e.g., not using the polypropylene pack in the initial pressing of the high density bale, or using second hand packs which would be reused (saving 3c/Kg); or vacuum packing in the cool stores, rather than at the pellet plant; or pressing bales to an exact weight. Some of these ideas are being explored further, but the most signifi cant cost improvements are likely to come from increasing pack density. Alpha losses in storage and transit have not yet been properly examined for this pack, although initial tests indicated that they would be significantly less than powder pellets under the same conditions. A trial is currently being set up to test this, and also the effect of samll pin holes which result in vacuum loss, but little oxygen entry. 49. Vacuum Packing Pressed Hops at High Density. By I.J. Edwards August, 1979 Suamary: Available information regarding the practical design and cost of presses to vacuum pack pressed hops up to normal pellet densities (approx 600-650Kg/nP) are presented. Large lost savings are apparent for vaciun packing hops up to about 500 Kg/m3 (c.f. 350 Kg/m3 at present) but beyobd this figure the returns drop as freight savings diminish press pressure increase exponentially. A doubling of press pressure increases density by approximately 100 Kg/m3 in this range in which we are interested. --_, __ lz ** perfectly practical to sell pressed hops in the present 25Kg pellet packs, at the same density as pellets. This could be achieved by a new fast action press costing about $30,000 or by a slower more conventional HD press costing about $10,000. Given the uncertainty regarding vacuum packing for the 1980 crop continue as at present. That is, using existing HD presses, but with some modifications to increase density and both customer acceptance and hop expasion though, it is reccnmended that provide a uniform weight. ' High Density Hop Baling - Reconnended Approach. Basic Benefits: Vacuum packing hops and selling to Europe or to Anerica reduces the freight cost from 60c (Dumped Bale) or 46<? (HD Bale) to a figure depending on the density - (current vacuum packs approx 29c/Kg) Density of 600Kg/m3 (ths same as pellets) would cost about 18c/Kg, a saving of 28c/Kg over HD bales or 5c/Kg over current vacuum packs, (properly compressed 50Kg) this represents a potential saving of approximately $110 000 over 311 tonnes 1979 crop exported in bale form. The Effect of Pressure on Density. FiS- 1Shows the effect on density of pressing whole hops. In the range in which we. are interested, it may be noted that an approximate doubling of pressure within the press increases the density by 100Kg/m3. In order to press to 600Kg.m3, we would need a pressure of approximately 600 psi ie 118 tonnes over an area the size of current HD Bales and vacuum packs, or 34 tonnes on the size of current 25Kg pellet packs (9 x 14"). In current vacuum packs the pressure is that of HD bales ie 22 tonnes (approx) over 441 sq" for 110 psi and 380 Kg/m3. Practical Problems Associated with Vacuum Packing Pressed Hops. The main interest problems are: 1. Time of pressing: Current HD bales require about 4-5 strokes of the ram to achieve required density, ie total stroke of about 35M The pressing time is about 20-30 minutes as a consequence of the slow movement of the hydraulic ram. Time of compression can be increased by: 50. Practical Problems Associated with Vacuum Packing Pressed Hops Cont'd: 1. (a) Double acting ram with a fast, low pressure stroke, followed by a slow, high pressure stroke for the final pressing - Cost about, $1,000 above single acting ram (F.Peacock data) and increase in speed estimate about 5-10 minutes per bale, ie a labour saving of approximately %C/Kg from a labour cost of approximately 2c/Kg. (b) Single hydraulic ram with a separate ram operating from compressed air (F.Peacock idea). Cost probably as much as (a) but faster speed. (c) 2 way compression with a low pressure initially compressing hops into a chamber vtere a high pressure ram operating at right angles (normally) to the first ram, completes the compression. Advantages: - Fast operation - only approximately 407. total str»ke of single ram. Disadvantages: Cost - probably 2x cost of 1 way press - probable cone breaking due to 2 way action. (d) Continuous pressing - in principal similar to a hay baler. Advantages - fast operation with compression all in one direction. Disadvantages - Unknown technical problems. 2. Spring back of compressed hops. After compression hops are normally contained in polypropylene packs which prevent expansion. It is desirable to vacuum pack the hops without the polypropylene pack, which in effect means that the pressed hops can only be placed in the pack for vacuum packing by the following means :- (a) Stripping the pack from the hops after some period of settling, as presently done with HD bales for vacuum packing. (b) Over-compressing the hops initially, and than inmediately vacuum packing after removal frcm the press chanber. Additional compression of about 30% would be required, corresponding to approximately 1207. extra pressure for 400Kg/m3, or 180% extra pressure for eOOKg/m3 (from fig.l). (c) Compressing the hops directly into a pouch and containing the sides until after sealing under vacuum - ie only the top able to expand between removal from the press and vacuum sealing. Additional compression of about 15% would be required, corresponding to approximately 60% extra pressue, for 400Kg/m3 or 90% extra pressure for 600Kg/m3. The Economics of the Alterations: Although we have only very tentative prices and inadequate knowledge of the effects of pressure on density and sponginess for newly dried hops, the basic data and costs would be as presented in the following table:- m Approx Density Cost $ Kg/m3 Bale Time to Cost of Cost of*.* Toatal Cost Wt (KG) Press Bale time/Kg Capital /Kg /Kg(cents) (cents) (min) 1. Normal H.D. Press (cents) 5000 380 100* 30 2.0 1.0 3.0 2. (1) with 2x Pressure 6000 480 125* 40 2.1 1.2 3.3 3. 7000 480 125* 30 1.6 1.4 3.0 8000 530 140* 40 1.9 1.6 3.5 4. (3) with chamber 9" X 14" 7000 700 25** 20 5.3 1.4 6.7 5. Ginger type press 0000 650 25** 10 2.7 6.0 8.7 (2) with 2 way ram 4. (2) with 4x Pressure & 2 way ram * Pack the same size as standard H.D. Bale. ** Current pellet pack filled with pressed hops. *** Capital cost in column 1 @ 20%, interest and 100 tonnes per year. 52. Note. (1) = Standard High density Press as used at present. Accurate cost quoted by Bremner, Scottsdale. (2) = Basically a standard H.D. press, but with 50%, increased ram pressure, and 507, reduction in speed for 1007, increases in total pressure. (3) = Basically (2) but with a double hydraulic pump so that ram is fast moving for part of the stroke, and slow moving when the pressure is high. (4) = Further increasing of pressure by a combination of stroke speed reduction and greater ram capacity. (5) = Press with 2 way action similar to Ginger press at Buderim. THE PRACTICALITY AND COST OF VACUUM PACKING UNDER THE VARIOUS ALTERNATIVES: Although costs outlined in table 1. are very approximate, it is now possible to examine with some objectivity. The following table is complied from table (1) and the paper "Vacuum Pack Pressed Hops .. 1979 report." by I.J. Edwards. Table 2. Pack Wt Kg Density Cost of initial Cost of Packaging Vacuum Packing Freight Kg/m3 Pressing/Kg. Pouch and Carton Cents/Kg Labour (cents/ (cents/Kg) Kg) Total 41 1. Present Vacuum Pack. 43.3 330 5* 6.3 5.6# 29 2. (1) with greater density 65 530 8** 4.2 3.7## 20 28 25 600 7*** 5.7 4.6### 17 27 25 600 9*** 5.7 4.6### 17 27 3. Pack same as pellets, from H.D. Press 4. Pack same as pellets from Ginger type press. * Assuming press already present, cost of pack (3«?) and additional labour cost above standard bales. ** Cost for item pack, labour and capital on new H.D. press, with 4+ pressure 12 way ra-. *** Cost for items 4 and 5 from table 1 assuming no initial pressing into polypropylene pack is necessary. # Actual cost 1979 ## Based on actual cost 1979 with proportional cost ready for extra Wt/pack. ### Actual pellet cost 1979 - Assume same for pressed hops. v General cargo rate to Europe or N. America. in oo 54. The follwing points now emerge: 1. There is not a big advantage in pressing beyond about 500Kg/nP. Above this level the marginal freight savings with increasing density are small (3c betwenn 500 and 650Kg/m3) and the pressure and costs necessary to increase the dinsity are high. 2. The loss of density due to rapid expansion of the hops following compression is considerable eg HD bales expand from approximately 20" square in the press chamber to 21" square in the packs and frcm 36-38 or 39" in length, (about 20% increase in value). The bale pressed at Buderim to 650Kg/m3 expanded approximately 15% after being held in the press for 5 minutes. 20% increase volume corresponds to about 100Kg/m3 in the density range in which we are interested. This means that if expansion could be prevented the effect would be the same as a doubling of the ram pressure. 3. A single super HD press at one location would seriously limit the flexibility of hop selection for vacuum packing unless content varies between growers in a largely unpredicable way from year to year, it would be undesirable to site such a press at the location of a single grower. 4. The economics of a fast speed, expensive 2 way press and a slower, cheaper, more conventional HD presses do not differ greatly. Conclusions:- Vacuum packing pressed hops is the logical and inevitable way to export hops - using ambient temperature transit and storage with miniinum alpha acid loss. The reaction of potential customers to vacuum packing is yet un-clear despite sales of 50 tonnes in 1979. Nor is the alpha loss in vacuum packs of pressed hops (c.f. pellets) knowi accurately. Given the conservative nature of many brewers, it would seem prudent to explore customer preferences regarding size and density before expending large sums on new presses ie to continue with the pressed pack with some up grading for the next season. After another season the acceptability of pressed hops under vacuum at high dinsitites and the need for new presses to cope with expanding acreage should be known more accurately. It is recannended that the decision to buy a new press be held over until 1980 and that vacuum packing of pressed hops continue more or less as at present for 1980. 55. The present pack using high density bales cut in half has advantages in simplicity and flexibility of source. Given a small increase in density, it would compete in price with more elaborate methods of vacuum packing under pressure similar to pellets. It would be relatively simple to upgrade the system as follows:- (a) Press into rigid boxes, to prevent all expansion or into a bale with a particle board insert each end to contain vertical expansion. (b) Leave overnight or longer, remove from containing bale and vacuum pack as at present. In this way instead of the average 43.3Kg pack it should be possible to about 50-55Kg per carton using conventional existing HD presses. 7T/0 56 57. WHOLE HOP PELLETS 1979 REPORT. I.J. EDWARDS, MAY, 1979. 58. BUSHY PARK PELLET PLANT: Whole Hop Pellets: 20/4/'79 Input 124,652 Output 124,652 Loss: Nil. 414 Kg. (cf. 200 1978) :per production hour ;e output per hour - 22/5/'79. = 386 Kg. (cf. 190 1978) Lost time = 22 hrs. _= 7%, Caused primarily by blocked rotary valve. Average Alpha: 9.14 Average Moisture: 8.75 59. LOSS: No samples were analysed to determine alpha loss in whole hop pellets. Careful analysis in 1978 showed no loss at all during pelleting, (baled hops were fed directly into the force feed of the die). In 1979 hops were fed to the die via the bale breaker and pneumatic system as used for powder pellets, but without using the harmermill, and bypassing the normal series of cyclones, conveyors and mijcing tank. Seme slight loss might be expected here. Dry matter loss was not determined for vAiole hop pellets in isolation, because the cut off point between powder pellets and whole hop pellets was not able to be accurately determined (due to unknown weight of baled hops stored in the pellet plant). The overall loss of weight assuming no loss at all in the whole hop pellets amounts to only 2.6% for powder pellets, indicating that little or no loss occurred in the whole hop pellets. In practice a small evaporative loss of 0.2 - 0.5% probably occurred plus a small loss in dirt and extraneous matter removed at the ducting intake. This would have been negated by moisture gains in bales between weighing at receipt in the cool store, and subsequent processing at the pellet plant up to 6 weeks later. 60. Technical Considerations Regarding Production of Whole Hop Pellets 1. Pellet Particle Size: A number of determinations on particle size were made by Allison Labs. following appendix. These are presented in the The following points emerge:- (a) Pellets produced frcm baled hops have slightly better particle size distribution than from bulk hops. (b) Pellets produced from bulk hops using the harrmermill without any screens produce unsatisfactory pellets. 2. Tteoughput: In 1978 maximum throughput was approximately 200 kg./hr. The maximum rate was due to the impossiblity of getting more than this quantity into the die. Entry was restricted by 2 scraper arms, by the die rollers, and by a rod separating the 2 rollers. This year the rate was increased to 4D kg./hr. primarily by removing one of the scraper arms. A more even feed rate may also have contributed. It is proposed to further increase throughput for 1980 by additional clearance of die obstructions. This will be achieved by buying a new die with %" more internal diameter, by altering the second scraper arm, and by reducing the size of the roller separator. Die amperage averaged only 35-40 for the 1979 season, and pellet temperature 40-45 C. By increasing throughput into the die, density of pellets is expected to suffer but particle size distribution to improve. The proposed new die is designed for better density (hole length 1 3/4" ->3") and it is anticipated that whole hop pellets will eventually be produced at throughput rates approaching those now current for powder pellets. 3. Pellet Density: Average pellet density in 1979 was 440 Kg./m (20 kg. in normal 25 kg. powder pellet pack). On two occasions the density was reduced to 400 kg./m3 (18 kg. in pack for a total of 144 cartons). These periods corresponded to high alpha (max. 10.8) indicating that density is worsened by high alpha acid. Since freight to Europe or to Anerica is based on volume, the reduction in weight from 25 kg. to 20 kg. with the whole hop pellet means that, in general cargo containers, the freight* is increased frcm 18c/kg. to 22.5c/kg. i.e. each additional 1 kg. that can be squeezed into cartons, saves approximately lc/kg. in overseas freight. It is anticipated that a better die will enable some improvements to be made in density of whole hop pellets. 61. 4. Plant Modifications for Whole Hop Pellets: The pellet plant as designed for powder pellets is unsatisfactory for whole hop pellets because (a) Excessive cone breakage occurs, especially in the mixing tank. (b) (c) Rotary valves are inadequate for increased volune associated with whole hop pellets (hanmer milled powder is much more dense than loose hop cones). Bridging frequently occurs in cyclones and mixing tank. To overcome the inherent problems, a new cyclone with a large rotary valve was constructed in 1979. This was installed just above the auger feeding into the pellet die, thereby by passing the two normal cyclones, 3 auger conveyors and the mixing tank. This arrangement works satisfactorily regarding iniformity of feed rate, minimum cone breakage and ease of operation. It has unfortunately, given some trouble in regard to seizing the rotary valve due to resin build up. Edifications to eliminate this problem are in hand. 62. General Ctanments: Whole hop pellets sales increased dramatically in 1979 over 1978 sales of 26 tonnes. This was primarily due to the enthusiasm of Guinness Dublin who found them a very satisfactory alternative to baled hops. The new cyclone installed to facilitate \<bole hop pellet production posed some inital technical problems, but these were gradually sorted out with the exception of the rotary valve, which continued to cause hold ups due to blockage with resin. It is confidently expected that with further modifications to the rotary valve, and installation of a new die with better density characteristics, whole hop pellet production will be streamlined to a routine operation with throughput rates and labour costs approximating those current for powder pellets. Throughput rates for whole hop pellets frcm bulk hops were considerably below those for pressed hops, with the result that the hanmermill was run without screens for some of the producticn from bulk hops. Resultant particle sizes were only slightly below a control without the hanmermill when the hops were very dry (4-6%) but damp hops (8-11%) fractured excessively in the hanmermill, and also resulted in large resin build up in the body of the hanmermill. Although it is anticipated that entry of bulk hops to the die will be improved for 1980, it is reconmended that all whole hop pellets in future be pelleted from bales, since these give better particle size distribution as well as better throughput rates. 63. ALLISON LABORATORIES AUSTRALIAN HOP MARKETERS SIZINGS LAB NO. SAMPLE WHOLE HOP PELLETS 4/6/'79. /lirm >0.3 (0.3 Bulk hops and hanmer mill i (very dry hops) 46 10 Figures • 44 % m/m Bulk and hanmer mill (very dry heps) 41 13 46 Bulk 49 11 40 Bales 50 11 39 ^4.0 >2.8 >_.4 703 /03 10 21 44 3.2 11.5 30.5 49.9 >4.0 72.8 71.4 Bulk 22 Bulk h_irmer milled 4.9 3 C.4 Bulk hanmer milled 55-1 2.5 2.1 8.9 86.5 Bulk hanmer milled 57-3 2.1 1.7 9.2 87 Bulk hanmer milled 54-6 3.7 1.6 8.2 86.5 33.3 1.5 6.9 58.3 31.7 0.04 7.1 61.2 . < Standard bales 56-1 Standard bales 52-8 6A. HOP GROWING IN TASMANIA. Some Notes on Cultural Practices and Timing of Operations. ( I.J. Edwards,J.P. Versluys ) September,1979. Introduction. In the following we have described the method and timing of operations carried out on the majority of Tasmanian hop properties. There are,naturally,quite a number of variations that occur between properties with regard to detail and timing of each of these operations. In the following,an 'acre' is a ground-acre,e.g. 900 plants at a spacing of 7' x 7'. Weed Control. Dock: Reglone/Gramorone in May/June;depending on weed cover a mixture of 2-4 pts./acre in 30 gals./acre. Asulox in early August;2-3 pts./acre in 20 gals./ Spring-applic.: Gramoxone/Reglone/Simazine during first half of Post-harvest: acre. August;e.g. 2 pts.Gramoxone,1 pt. Reglone,3-4 pts. 'Gesaprim' (flowable product containing 50# Simazine) in 30-40 gals, water per acre. Roundup: Some experimentation with Roundup during the last two years,as a replacement for Gramoxone/Reglone/ Simazine mixtures,has given promising results at the rate of 1 pt./acre and with addition of 2# (weight/volume) of Sulphate of Ammonia.Roundup can only be used with any degree of safety while the hops are quite dormant;even then,some cases of damage have been recorded following its use on medium/light soils. Pruning & Cultivation. No pruning takes place in Tasmania. In N.E. Tas. no ground- disturbance takes place at all. In S. Tas. light/medium cultivation is general and non-cultivation,where tried,has given poor results. Non-cultivation could probably be made to work on most sites in S. Tas.,but cultivation costs about the same as herbicide treat ments. Yield differences between S. Tas. and non-cultivated areas in N.E. Tas. are not sufficiently conclusive to promote noncultivation regardlessly. 65. However,it should be stressed that both pruning and soil cultivation are absolutely 'ideal' agents to transmit disease organisms,if present,and their carriers.With the appearance,in parts of Victoria last year,of some disease symptoms which may have been due to virus infections,and with the certainty that root-rot organisms are present in all Victorian hop grounds, cultivations should be kept to an absolute minimum and non- cultivation should be tried seriously,(as is being done on a small scale at the Tobacco Research Station). Soil analysis in N.E. Tas. has shown that non-cultivation favours the build-up of organic matter in the soil.S. Tas. hop soils are generally older,with less organic matter and with a soil structure such that compaction takes place in wet soils,(tractors, livestock). Cultivation through the growing season with a tiller,working in one direction only.The depth of tilling is restricted to the surface 2-4" in order to minimize damage to the hop root system. Fertilizers. A base application of Superphosphate and Muriate of Potash takes place in August/September;mostly as standard mixes prepared by the E.Z. Company.Quantities applied are determined on the basis of soil analysis results and vary from 6-14 cwt./acre.Most Growers apply the total quantity of base fertilizer in one dressing;some (e.g. Ferguson on very porous Springfield soil) split the base dressing in two or even three applications,with the last one going on in late December/early January. Lime: most Growers in S. Tas. (average pH 6.5) use 1 Tonne/acre agricultural lime or Dolomite every other year.Growers in N.E. Tas. apply 1 Tonne/acre of Dolomite every year (average pH 5.7). Nitrogenous Fertilizer:most Tasmanian Growers use Nitram only; quantities very widely per paddock and per location from 4-10 cwt./acre.Ground application of Nitram is generally preferred above application through overhead irrigation systems. 66. Foliar fertilizers: Recommendations for the 1979/80 season include the use of a complete foliar fertilizer.A minimum of three and preferably four applications usjing a total of approx. 1_ gallons per acre of one of the proprietary brands (Wuxal,Poliverdol,etc.). Important that at least one of the applications takes place before burring;the last application goes on about a fortnight before harvest,the remaining one or two applications are spaced evenly in between.Foliar fertilizer applications may be combined with miticide applications. Stringing. At any time during August/September but usually after application of the Gramoxone/Reglone/Simazine weedicide mixture. Stringing crews of 6-7 people.Day labour in N.E. Tas. (cost approx. $55 - $65/acre) and contract rate in Bushy Park ($55/acre for 1979). Training. Day labour in N.E. Tas. (cost approx. $60-$80/acre),and contract rate in Bushy Park ($75/acre). Most Growers start training when approx. 80# of hills can be fully furnished (3x3 vines).The earliest vines are trained, there is no 'pulling'.At Bushy Park,rank vines ('bull-runners'), which have climbed up the strings before the training proper commences,are pushed down the strings with forked sticks.Bullrunners do not occur in the N.E. Tas. hop area,possibly as a result of the non-cultivation practice. Defoliation. In some cultivation areas,excess vine left after training is covered over with a mulboard plow.On most properties however, excess vine is not removed until sheep are introduced in the paddocks during late December/January,or until chemical defoliatior takes place in early-December. Important that sheep are taken out of the hop paddocks some 2-3 weeks before harvest so that some hop re-growth can take place from the base of the hill and from the lower 3-4 ft. of vine. This new growth continues to function after the vines have been cut and,until natural dormancy sets in,this will contribute towards rootstock reserves. 67. Chemical defoliation has been safely carried out when the vines are some 12 ft. up the strings.A mixture of 6 pts. concentrated sulphuric acid and 3_ pts. DNBP in 100 gals, of water will cover approx. 3 acres.Floodjets are used to cover the base of the hills and the lower 3-4 ft. of vine. Irrigation. Mostly overhead sprinklers in S.Tas. and flood in N.E.Tas. Sprinkler irrigation either permanent overhead (most of Bushy Park Estates) or portable overhead (N.Edwards) from fixed mains with flexible $" hose with sprinkler jets hooked on the top wires of the trellis system. There is no measurable yield difference between hop grounds irrigated from overhead sprinklers or from furrows. Irrigation starts as required,depending on area and season.In the South,rainfall is 20-30" per annum,and more irrigation is required than in N.E. Tas. where the annual rainfall varies from 40-45".Water requirements of hops are generally considered to be much the same as those of other horticultural crops,and transpiration figures from the Dept. of Agriculture provide a guide to requirements which average about 1" per week through the growing season. Following rain during the summer months it is important to begin irrigating again within a few days to ensure that the last areas to be watered in any sequence do not suffer water stress. Nearly all areas have adequate water for irrigation,even in dry years,without recourse to dams and certainly not to draglines. Spider Control. There is considerable difference in the attitude of hop Growers toward spider control.Some Growers,mainly the larger ones,are inclined to spray in a set sequence regardless of whether mites are evident or not.Others either do not spray at all,or only after serious damage has occurred. There is some evidence that,in the absence of spraying,natural control (predatory Stethorus beetles) maintains a rough balance which can be upset by spray applications which either kill the predators directly,or kill them indirectly by removing their food.This thinking has led some Growers to omit spraying from 68. hop programme with relative safety in some years,but with serious infestations in other years. The introduction of the mite-monitoring service by A.H.M. in 1979 offered Growers the means to rationalize the spraying procedure,by applying miticides only as required.This service gradually gained acceptance and should enable proper control measures to be adopted with some objectivity in 1980. The mite problem is quite variable,with great variations in the degree of infestations from Grower to Grower,paddock to paddock,and year to year.Killing success from individual sprayapplications varies due to a combination of mite resistance and spray coverage. (Several instances of mite-resistance to Tedion/ -elthane mixtures were recorded in N.E. Tas. during 1979/80. A report -by an interested Chemical Company- of mite resistance in Victorian apple orchards to Plictran still needs verification. With that possibility in mind,a Bayer-product,Peropal 25,will be tested on hop mites during the coming season.) Air blast sprayers are generally considered better than low volume applicators with high pressure jets. Spraying generally follows the following pattern: 1._elthane,1-1_ pts./acre in the early-spring application of herbicides{effectiveness has not been properly assessed,but low cost{intended to kill overwintering females.' 2.Early applications,October-December. Tedion and Kelthane,1 litre of each per acre. 3.Later applications,December-March. Plictran,12 ozs./acre. (For addition of foliar fertilizers to miticides see'Fertilizers'.)