Product datasheet
Anti-ACADM antibody (Biotin) ab212024
1 Image
Overview
Product name
Anti-ACADM antibody (Biotin)
Description
Goat polyclonal to ACADM (Biotin)
Conjugation
Biotin
Specificity
ab212024 is expected to recognise reported isoforms (NP_000007.1; NP_001120800.1).
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
Immunogen
Synthetic peptide corresponding to Human ACADM aa 408-421 (C terminal) (Cysteine residue).
(NP_000007.1).
Sequence:
RLIVAREHIDKYKN
Database link: P11310
Run BLAST with
Positive control
Run BLAST with
Human heart lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Purification notes
ab212024 was purified from goat serum by ammonium sulphate precipitation followed by
antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab212024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 45 kDa
(predicted molecular weight: 47 kDa).
Target
Function
This enzyme is specific for acyl chain lengths of 4 to 16.
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Involvement in disease
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency
(ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting
hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
Cellular localization
Mitochondrion matrix.
Anti-ACADM antibody (Biotin) images
Anti-ACADM antibody (Biotin) (ab212024) at
3 µg/ml + Human heart lysate (in RIPA buffer)
at 35 µg
developed using the ECL technique
Predicted band size : 47 kDa
Primary incubation was 1 hour. Detected by
chemiluminescence, using streptavidin-HRP
and using NAP blocker as a substitute for
skimmed milk.
Western blot - Anti-ACADM antibody (Biotin)
(ab212024)
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