iuioi, VcuuetaIIe Dhiesf Document coUectiOn Oregon State University, April 1979 Volume 28 Number 2 Oregofl coUect0fl Green bean lines tested in 1978 Results of the OSU green bean breeding program were encouraging in 1978. Of 31 breeding lines tested extensively in plot yield trials at Corvallis, several look very promising for yield, quality and processing characteristics. Our immediate objectives the last few years have been to develop one or more varieties to replace Oregon 1604. This would require a variety with yielding ability close to or better than that of Oregon 1604, a better growth habit, and straighter and smoother pods with equal quality. Many lines tested and discarded since Oregon 1604 was released have been superior in some respects but not equal in yield. A number of our newer selections appear IN this issue.. Green Bean Lines Tested in 1978 Nitrogen- Fixing Bacteria, Nitrogen Fertilizer Tested on Bush Beans to meet or even exceed the yield objective. The breeding program has always stressed the Blue Lake type, which is useful for both canning and freezing. New lines therefore, are evaluated for both canning and freezing quality at OSU and by several processing companies. A number of bean growers have been involved in trials of various sizes for evaluation of field performance and to determine if the lines are well adapted to mechanical picking. Root rot resistance is evaluated in plots at Corvallis, and specific crosses are made to transmit resistance into the better processing types. However, all or most of the resistance usually is lost when beans are selected for earliness, high pod yield and quality. Lines resistant to white mold are likely to become available from another experiment station in about a year, and will be used in our breeding program as soon as possible. Tables 1 and 2 show yield data for all 31 OSU lines tested in 1978, and a number of lines or varieties from seed companies. Tons/acre shown in the tables Table 1. Green Bean Yields - Firat Planting (May 30, 1918), Corvallis, Oregon. flarveat 1 No. 1 Parentage or source Line Ore 16048 ::::::: 8 439.- 9 4755-1 4755-2 4773-2 4794 4843-1 4843-2 4844 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 38 39 4845-2- 4846 4849-1 4849-2 4849-3 4850 4852-28 4862 4880 4882 4883 4884 4886 4895 49078 49118 E. Gallatin BB1 73149 Vitagreen Exp. 186 Exp. 519E-6 15202 E6211 40 WIIP-1 WhIP-) 41 ISIh-6 42 Advance 85 1386519-3-2 U 86571-5-2-1 86625-1-1-5-1 86647-6-3-1 86647-6-3-1-1 86647-6-4-1 86647-6-5-1 86647-6-5-2 86647-8-2 86843-2 B6849-1-3 86849-3-1 86849-3 86849-8-18 B6849-9B 86862-1-1-1 86897-1-1-3-1 86897-1-1-4-1 Gallatin Valley Rogers North. King North. King Ferry Horse Id. Seed Bean Aegrow 43 881. 53 44 NCX 8010 NCX 8013 Slenderette Code 129 Code 160 Charter Clamor Callatin Valley K9398 Keyatoe 45 46 47 48 49 50 1 FMC Siee adj 2 1-4 Days ay. tuna 62 89 7.9 63 d . harvest 2 tone 8: 8.6 11.8 9.9 92 7.0 8.4 8.2 64 89 6.9 63 96 64 97 64 64 64 64 98 97 7.6 5.9 7.3 64 64 64 64 65 65 64 62 62 91 93 92 84 85 9.0 91 7.8 3.3 7.0 5.2 3.2 6.1 5.2 6.7 6.8 7.0 5.0 6.5 7.6 6.6 5.5 4.5 6.4 5.2 7.8 8.8 91 65 65 62 63 65 65 63 65 69 65 98 64 72 63 99 95 65 65 65 7.4 7.5 6.6 6.6 64 64 7.3 7.0 86 90 88 94 85 86 85 93 62 96 45 88 85 96 91 97 75 87 77 89 60 tonb 2 1-4 Days tons 7.8 8.9 8.6 7.1 cv. *1 :j' 10.4 64 64 93 60 87 cv. .: 7.3 8.3 64 64 64 64 64 Days Sieve adj. 68 : 65 Sieve 2 1-4 9.1 12.0 11.6 9.6 11.1 8.7 10.8 10.7 7.9 9.9 12.2 10.2 9.2 9.1 11.2 12.0 11.7 9.6 11.0 4.7 7.8 7.3 . 68 65 68 49 58 43 66 66 66 66 65 66 66 66 66 66 65 65 65 65 65 82 87 81 77 86 65 68 68 68 64 64 69 84 82 90 75 73 73 86 79 80 72 76 49 47 37 84 8.9 8.4 9.8 8.0 8.7 8.1 9.6 9.4 7.5 7.8 8.5 7.4 9.0 8.9 7.2 8.7 8.9 8.9 8.6 10.2 10.5 8.9 59 5.8 8.0 68 68 47 64 77 8.6 6.6 6.4 9.2 65 77 9.2 10.2 68 68 7.0 9.6 4.7 8.9 Yield adjusted by stand + sieve Harvest 3 111K 8,8 9.1 9.1 10.6 68 69 69 11.9 10.6 12.2 12.8 10.0 10.3 11.9 9.2 11.1 10.9 9.8 11.2 11.6 10.9 10.8 10.1 10.2 7.8 7.8 8.7 8.3 7.5 69 68 * 33 52 50 39 38 68 34 68 48 68 68 68 68 47 49 42 38 Coal adj. tons 7.5 .-: ' 8 12.0 9.8 10.3 10.6 11.3 10.2 11.8 9.6 10.7 10.9 11.3 35 9.5 flsrv. no. adj3 tong 2 11.0 I1T 136 129 117 126 128 112 136 130 136 137 142 133 121 142 126 134 134 140 130 10.0 10.0 10.3 9.4 9.9 8.6 11.6 L4 10.4 10.8 10.4 - 69 Av. stand 8.1 3 .7 2 0.3 8.7 2 9.4 2 9.2 10.0 10.2 10.6 9.3 9.9 10.0 10.1 11.8 3 3 2 3 3 2 2 2 2 2 3 1 3 3 3 3 2 126 94 122 79 121 116 2 83 60 3 3 7.4 2 9.3 10.0 6.7 5.7 9.3 10.3 2 2 3 2 2 2 70 80 6.7 8.8 7.7 9.8 68 47 9.0 8.7 50 80 9.0 8.3 9.0 10.8 70 73 6.1 9.8 7.5 69 59 39 10.7 65 68 11.7 6.9 9.3 124 111 9.0 68 40 65 74 5.6 7.2 110 128 1 6.7 6.7 9.3 6.6 10.8 9.7 65 68 68 68 69 93 66 6.2 5.8 6.7 8.4 6.6 7.6 8.8 111 117 137 112 3 2 140 2 45 7.8 9.5 55 52 45 9.6 9.7 6.9 7.8 8.4 69 68 70 69 70 36 84 57 33 48 6.7 6.8 9.6 7.8 8.0 5.8 9.1 10.3 6.5 7.8 83 11.6 8.4 10.4 10.6 10.4 138 120 136 99 113 8.1 4.9 9.2 11.1 Means of 4 replications; subplots of 51 were harvested from 20' plots on each harvest date; rows 36" apart. Tons/acre adjusted up or down by the percent deviation from 50% 1-4 sieve Yield adjusted to 50% 1-4 sieve aize, then adjusted for stand by Analysis sizes. of CovarlanCe. LSD @ 5% significance - 2.16 tons/acre. 1 3 2 1 3 3 10.0 10.3 7.6 8.4 8.9 8.4 7.8 8.1 5.4 8.8 9.2 12.1 3 8.0 8.3 Table 2. Green Bean YieJ.ds - Second Planting (June 14, 1978), Corvallis, Oregon.1 Unadjuaced Yisld Toa adjuated for No. Line Parenc&g. or source 12 13 14 Ore 1604B Ore 1604 4091-3 W 4091-3 C 4091-3 4094-1 4094-3 4439-5 4755-L 4755-2 4773-2 4794 4843-1 4843-2 15 16 4844 36647-6-3-1-]. 4845-2 86647-6-4-1 1 2 3 4 5 6 7 8 9 10 II 85713-I 85713-4 85713-2 86519-3-2 86571-5-2 36625-1-1.-S 86647-6-3-1 2 1-4 0aya 62 62 62 62 62 63 sieve2 sv. tons 60 9.1 6.9 8.1 5.9 4.6 3.9 6.7 6.1 35 32 64 7.1 7.3 3.4 4.9 52 38 63 62 65 65 63 63 53 5.5 5.6 6.1 6.2 5.3 43 6.2 64 64 64 64 55 57 48 47 6.3 7.6 5.5 3.9 49 62 60 4.8 4.8 5.6 6.8 6.8 5.5 5.8 6.6 8.1 5.4 3.8 4 Pod set score eieve3+ stand Av. 7.8 142 150 130 136 146 123 145 145 147 148 136 5.0 5.0 4.5 4.0 4.5 4.5 4.5 4.0 133 5.0 149 138 148 138 2.5 3.5 3.5 2.5 5.6 2.7 6.6 6.1 5.0 4.6 5.3 6.4 6.9 4.7 5.2 6.2 8.4 5.7 3.1 tud 1.5 4.0 3.5 6 18 19 2* 21 22 24 25 26 27 28 29 30 31 32 33 34 4849-1 4:49-2 4: 9-3 4850 4852-2 s 36647-6-5-1 4.62 4880 4882 4883 86843-2 36849-1-3 36849-3-1 86849-3 36849-8-1 B 86849-9 3 86862-1-1-1 86897-1-1-3 86897-1-1-4 Callarin Val. 488.4 4886 4896 4907 5 4911 B E. Gallatia BBL 73149 Vitagreen 64 86647-6-5-2 86647-:- 2 P.ogers '.. ____________________________________ 6 6 ____________________________________________________ . . . . . . . . . . . . . . . . . . . . . . . . . : : . : . 1orthKing :. 4.0 5.0 5.0 4.0 4.5 139 3.7 143 143 126 4.5 2.5 2.5 4.5 140 3.0 95 . . 145 147 150 146 150 . 519E-6 3 E5 202 Ferry More e 61 ' .0 _____________________________________ iHP-1 .4 Ld. Seed Bean _p*__._ 4 SB-. 42 43 44 45 46 47 48 49 50 Advance BEL 53 NCX 8010 NCX 8013 Slenderette Coda 129 Code 160 Calamor K9398 ..: A.egrow FMC Charter Galiarin Val. KeyStone 66 68 41 53 64 45 63 63 64 63 61 65 63 61 8.1 6.9 6.1 4.4 5.9 5.1 4.4 5.9 3.0 70 48 34 38 7.4 7.1 5.8 4.9 7.1 5.2 3.7 6.5 2.6 7.4 7.3 5.9 4.7 6.6 5.0 1.6 7.6 2.6 142 139 115 143 126 124 143 99 142 Means of 4 replication; single harvest of 5' froR a 20 ft plot, rows 36" apart. Tons/acre adjusted by the % deviation from 50Z 1-4 sieve sizes; LSD 8 5Z significance - 1.98 tons/acre. Yield adjusted to 50% 1-4 sieve, then adjusted for stand by analysis of Covariance. ' Average of scores assigned by Baggetr, Frazier, and Stang, for apparent split-set tendency; 1 poor set 5 - good set. 3 5.0 4.0 4.0 2.0 4.0 4.0 1.0 4.0 2.0 are from hand-picked plots and are high in comparison to conunercial yields; thus, it is important to compare the experimental lines with varieties such as Oregon 1604. Following are descriptions and increase status of the more advanced individual OSU lines or groups of related lines considered most promising after the 1978 season: While they probably can be grown without problems under good growing conditions, trouble with fiber could occur if harvest was delayed. The growth habit is very upright when plants are young, but the plant is branchy and cannot stand up under the heavy pod load. 4883, 4886 - the best of a group of six related lines tested in 1978, these have medium length pods which are seldom crooked. Pod quality has been good in both canned and frozen samples. The plant is more upright and less branchy than Oregon 1604 and the pods are held off the ground until the pod load gets heavy. Plot yields in both 1977 and 1978 were equal to those of Oregon 1604, but maturity is about two days later. These two lines are currently receiving more interest than any of our other prospects, and seed increases in 1979 will be quite large. About 40 acres of 4883 trials will be planted in 1979, but only enough seed for about one acre is available for 4886. 4091-3 - similar to Oregon 1604 in having a large pod load for the size of plant, and almost as early, this line has smoother pods and a less branchy growth habit. Yields have varied from equal to 1604 to about 1 ton/acre less and quality usually has been rated higher It matures at a smaller sieve size than 1604; thus, it will be. important to harvest it on time. About 5,000 pounds of seed will be planted in Oregon in 1979 and 20,000 in 1980. OSU will either release or discontinue 4091 after the 1979 season. 4094-3 - related to 4091, but with larger seeds and pods, this line has shown exceptional pod quality, especially color, but is about two days later and does not always yield as well. A tendency for poor seed germination has caused reservations about its future, and only moderate sized trials are planned for 1979 and 1980. If 1979 trials are not very encouraging, it will be discontin- J.R. Baggett Horticulture Department G.W. Varseveld Food Science Department ued. 4755-1 and 4755-2 - these lines have a distinctly different upright growth habit, with long pods borne well off the ground. The pods have shown excellent freezing quality, but average canning quality. Pod fiber develops in the older stages but has not resulted in low quality scores thus far. Seed increases in 1979 will be small pending further quality evaluation. ****************** ..** Oregon Vegetable Digest is published four times a year by the Agricultural *Experiment Station, Oregon State Uni*versity, Corvallis, OR 97331, J.R. Davis *Director. Address correspondence to the *author concerned or to the Department of *Horticulture. Material may be re*printed providing no endorsement of a *coinmercial product is stated or implied. *Please credit Oregon State University. *To simplify technical terminology, .trade names of products or equipment *sometimes will be used. No endorsement *of products named is intended nor is *criticism implied of products not *mentioned. ** * * * * * * * * * * * * * * * * * * 4843-1, 4843-2, 4846 - this group of lines has the potential for high yields of small sieve pods which are straight and refined in the 3 and 4 sieve sizes. Older pods develop fiber, and although quality tests have been favorable, these lines are being evaluated carefully with only moderate seed increases. 4 * * * * * * * * * * * * * * * Nitrogen-fixing bacteria, nitrogen fertilizer, tested on bush beans Six field experiments were conducted at the OSU Vegetable Research Farm during 1975-1978 to determine the effects of inoculation with Rhizobiuin phaseoli bacteria and of nitrogen fertilizer rates on growth, nodulation and yield of Oregon 1604 bush snap beans. Experiments 1 and 5 were in an area which had no snap beans grown during the previous five years, with Experiment 5 on a loamy sand soil type. The other experiments were in areas in which snap beans had not been grown for one or two years and were on a silty clay loam soil, as was Experiment 1. A phosphorus-potassium fertilizer was banded at planting at a rate of about 45 pounds phosphorus and 85 pounds potassium per acre. Ammonium nitrate fertilizer was broadcast over the row after planting in appropriate plots and all plots were irrigated before or shortly after emergence of seedlings. Dyfonate was broadcast on the soil surface and incorporated before planting for symphylan control and trifluralin was broadcast and incorporated for weed control. Dinoseb was also applied preemergence for weed control. The seedcoating inoculant was added to slightly dampened seeds just before planting. The granular furrow inoculant was applied with a belt planter so inoculant was under the seeds in the furrow, and then inoculant and seeds were covered with soil. Treatments were replicated four or five times. Plots were irrigated by overhead sprinklers as needed at seven to 10-day intervals. Ten plants from each plot were removed by shovel with as much root system intact as possible at one week before bloom or after full bloom, and fresh weights and number of nodules were determined. There was no determination of nitrogen fixation. Once-over harvest of pods was by hand. Main effects of inoculation on yield were not significant in Experiment 1 (Table 1). However, there was a 15 percent increase in yield from inoculation when no captan seed treatment was used but a slight decrease from inoculation The captan treatwhen captan was used. ment increased total yield about 26 percent which was related to a 118 percent increase in number of plants per plot compared to no fungicide application. Because of the increase in plant population, weights of individual plants were lower from the captan treatment. At the highest nitrogen fertilizer rate; yield and plant weight were higher while numbers of large and small nodules were lower than at the zero nitrogen rate. There were no interactions of nitrogen x inoculation treatments in any of the experiments. In Experiment 2, where no fungicide seed treatment was used, nitrogen fertilizer application increased yield and the highest rate of nitrogen, 75 pounds per acre, decreased number of nodules per plant (Table 2). Yields were not significantly affected by inoculation treatments, but the se.edcoating inoculation treatment produced slight increases in yield and plant weight and in number of nodules per plant compared to the check with noinoculation. Yield and nodulation were lowest from the granular furrow inoculation treatment. Yields in Experiment 3 increased 11 percent from nitrogen fertilizer application, 75 pounds per acre, but number of nodules per plant averaged 20 percent less than when no nitrogen was applied (Table 3). There were slight but non-significant increases in yields and in number of nodules per plant from inoculation treatments. Three inoculation treatments, the same as in the above experiments, were at 0 and 35 pounds nitrogen per acre in Experiment 4 in 1976. There was no increase in yield from nitrogen application or from inoculation treatments (data not shown). Plant weights and nodulation counts were not taken. There was no increase in yield or in nodulation from seedcoating inoculation in Experiment 5 in 1977 in which inoculated and non-inoculated treatments were at nitrogen rates of 0, 15 and 60 pounds per acre. Also included was a treatment in which urea sprays were applied to foliage (three applications, approximately weekly intervals). The 15 and 60-pound nitrogen rates reduced the number of nodules 23 and 68 percent, respectively, compared to the 0 rate (Table 4). There was no difference in yield or number of nodules per plant when the 0 nitrogen rate and foliar spray treatment are compared. There was a slight but non-significant yield increase from 60 pounds nitrogen per acre compared to the treatment in which no nitrogen was applied. Several sources of seedcoating inoculants were used in Experiment 6 in 1978 at two nitrogen fertilizer rates, Table 1. 0 and 60 pounds nitrogen per acre. There was a small but non-significant increase in yield from the 60 pound nitrogen rate but this rate produced a significant decrease in average number of nodules per plant (Table 5). None of the seedcoating inoculants markedly increased yields over no inoculation although there were some trends for slight increases in yields and in nodulation of roots. Even though no nitrogen fertilizer was applied, increases in yield of beans from inoculation with Rhizobium were small and inconsistent but usually there was an increase in nodulation. Snap bean nodules did not appear to contribute much in providing nitrogen for plant growth and yield, however, no measurements were made on nitrogen fixation. Application of nitrogen fertilizer usually increased yield but decreased nodulation. Benefits from inoculation would be more likely in areas where snap beans had not been grown previously or where soil populations of Effects of fungicide (captan) seed treatment, nitrogen rates and inoculation on yield, sieve size distribution, plant weight and nodulation of Oregon 1604 snap beans. Experiment 1, 1975 T/A Treatments Yield 0 £ pods 1-4 sieve % No. nodules/plant wt/plant (g)Y largeZ 50 74 9 14 19 11 NSD 53 60 21 small Fungicide with without LSD .05 4.8 3.8 0.3 0.4 80 74 76 77 78 .01 3.6 4.4 4.8 0.3 0.4 Inoculation check seedcoating furrow 4.2 4.4 4.2 75 77 78 .01 4 28 44 10 13 N rates (lbs/A) 0 15 75 LSD .05 72 10 19 10 33 42 27 6 12 13 7 NSD 60 59 67 15 20 16 33 40 34 (No significant differences in means of inoculation treatments) Y fresh weight just after full bloom, Z nodules larger than 2 mm diameter. 6 Table 2. Effects of nitrogen rates and inoculation on yield, sieve size distribution, plant weight and nodulation of Oregon 1604 snap beans. Experiment 2, 1975 T/A Treatments Yield of pods % 1-4 sieve wtlpiant (g)Y No. nodules/plant largez small N rates (lbs/A) 0 15 75 LSD .05 3.5 3.6 4.0 LSD .01 NSD NSD Inoculation check seedcoating furrow 3.6 3.9 3.5 26 26 27 27 25 27 98 100 104 NSD NSD 6 3 42 50 32 NSD NSD 99 111 92 5 43 3 3 45 35 4 2 2 (No significant differences in means of inoculation treatments) Y fresh weight just after full bloom, Table 3. Z nodules larger than 2 mm diameter. Effects of nitrogen rates and inoculation on yield, sieve size distribution, plant weight and nodulation of Oregon 1604 snap beans. Experiment 3, 1975 Treatments T/A Yield of pods % 1-4 sieve No. nodules/plant wt/plant (g)Y largeZ small 46 38 N rates (lbs/A) 0 75 LSD .05 4.0 4.5 0.4 .01 NSD Inoculation check seedcoating furrow 4.2 4.4 4.1 58 57 35 19 49 14 10 13 NSD 58 38 59 45 43 55 5. 16 17 17 NSD NSD 38 44 43 (No significant differences in means of inoculation treatments) fresh weight just after full bloom, Z nodules larger than 2 mm diameter. 7 Table 4. Effects of nitrogen rates and inoculation on yield, sieve size distribution, plant weight, and nodulation of Oregon 1604 snap beans. Experiment 5, 1977 Yield 'F/A Treatments 0 f pods % 1-4 sieve wt/plant (g)X no. nodules /p lant>' N rates (lbs/A) 0 15 60 Foliar spray LSD .05 .01 Inoculation check seedcoatingZ 4.7 4.5 5.9 4.6 57 62 60 57 NSD NSD 5.1 59 59 4.7 14 16 16 15 22 17 NSD NSD 8 15 16 18 17 7 24 9 (No significant differences in means of inoculation treatments) X fresh weight one week before first bloom, y nodules larger than 1 mm diameter, Z seedcoating and granular furrow inoculants in Expt. 1-5 were supplied by the Nitragin Co., Milwaukee, Wisconsin. Table 5. Effects of nitrogen rates and inoculation on yield, sieve size distribution, plant weight, and nodulation of Oregon 1604 snap beans. Experiment 6, 1978 Yield 0 £ pods Treatments T/A % 1-4 sieve wt/plant (g) X no. nodules /2lanty N rates (lbs/A) 28 27 .01 5.9 6.2 NSD NSD Inoculation check seedcoating-1 seedcoating-2 seedcoating-3 seedcoating-4 seedcoating-5 6.2 5.8 5.7 6.0 6.6 6.2 27 28 27 28 27 27 0 60 LSD .05 14 14 20 6 3 NSD NSD 5 12 15 14 16 13 16 12 11 12 15 16 12 (No significant differences in means of inoculation treatments) X fresh weight one week before bloom, y nodules larger than 1 mm diameter, seedcoating 1 and 2 supplied by the Nitragin Co., Milwaukee, Wisconsin; seedcoating 3=MS 212, 4=Moly-Co-Thi, supplied by North American Plant Breeders, Princeton, Illinois; seedcoating 5 supplied by dAT, Cali, Colombia, S.A. Z 8 ment of more responsive varieties to inoculation and in development of more If these effective strains of Rhizobia. efforts are successful and increased nodulation will increase yields, then inoculation probably will become more (Some of the data precommonly used. sented are part of a MS thesis by Mei Ma, Oregon State University, 1978). beneficial strains of Rhizobia were These aspects were not studied. low. Compatibility of inoculants with various seed protectants of fungicides and insecticides also needs to be considered. Different species and cultivars of beans show variable nitrogen fixation abilities and some of the indeterminate or climbing types, of dry beans especially, have been reported to be more efficient than the shorter season, determinate types. Emphasis is being placed in some breeding and improvement programs on identificatioi or develop- Mei-Hwa Wang Ma and H.J. Mack Horticulture Department Vegetable Notes most striking feature of the ripening mutants is their prolonged shelf life. Appropriate F1 hybrids are currently being developed to further quantify the mutant hybrid effects and establish the feasibility of exploiting these unusual mutants in the development of improved fresh and processing varieties of toma1978). (HortScience 13:508-513. toes. In work on two varieties of dry beans, Subhadrabandhu, Dennis and Adams in Michigan found that persisting and abscising pods could not be distinguished on the basis of length during the first three days after flower opening; however, the growth rate of abscising pods began to decrease thereafter and size differences between persisting and abscising pods increased until the smaller pods dropped. They concluded that levels of extractable abscisic acid, phaseic acid and dehydrophaseic acid do not regulate fruit abscission in dry beans. (J. Amer. Soc. Hort. Sci. 103:565-567. 1978). In a symposium on increasing the biological efficiency of vegetable crops, topics included were: genetic potential for overcoming physiological limitations on adaptability, yield and quality in the tomato; photosynthetic efficiency and crop production; potential for nitrogen fixation in vegetables; isolating plant germplasm with altered efficiencies in mineral nutrition; and breeding vegetables tolerant to environmental stress. (HortScience 13:671- Water stress at silking of the sweet corn inbred 'Luther Hill' significantly reduced both seed yields and subsequent seed vigor index compared to moisture stress three and six weeks after silking. Marketable seed and seed size distribution were unaffected by any of the stress treatments according to El-Forgany and Makus who conducted the work at the University of Idaho. (J. Amer. Soc. Hort. Sci. 104: 102-104. 686. 1978). Tissue culture techniques for propagation of gynoecious cucumber plants using axillary buds offers a greater chance of obtaining a desirable selection from the field than does the traditional method of rooting cuttings. Handley and Chambliss in Alabama further suggest that bud culture has a fivefold advantage since five or six axillary buds can be obtained from one cutting. (HortScience 14:22-23. 1979). 1979). . Tigchelaar, McGlasson and Buescher indicate that the identification of several ripening mutants in tomato has provided a new tool for examining the regulation of tomato ripening. The 9 AT. BULK uss privat. for P.n.Ity PubUciflon Dlrsctor Class Third 101 AGR AGRICULTURE OF DEPARTMENT U.S. PAID POSTAGE 97331 Oregon Corvallis, University State Oregon of STATION EXPERIMENT AGRICULTURAL