JUNE 1934 STATION BULLETIN 328 Studies with Alfalfa Hay for Milk Production 'C Agricultural Experiment Station Oregon State Agricultural College CORVALLIS FOREWORD first became an important crop in the ALFALFA western states in the seventies and eighties. Since that time its culture has spread to the central and eastern states. In 1930 more than 68 per cent of all the tame hay produced in eleven western states was alfalfa. The development of the dairy industry of the western irrigated sections of the United States has closely followed the increased growing of alfalfa. Owing to the relatively low costs of producing alfalfa hay in the irrigated sections, it has become a general practice to restrict dairy cattle more or less largely to alfalfa hay. This practice has given rise to a number of nutritional and economic questions which are not encountered where alfalfa merely constitutes the principal roughage in well-balanced rations. In these nutritional and economic problems the Oregon Agricultural Experiment Station has been interested for a long time, and in 1924, began an investigation, the results of which are reported in this bulletin. TABLE OF CONTENTS Page Introduction 6 Review of Literature 6 Experimental 7 Objects of the Investigation Preliminary Investigation Main Investigation Plan of Investigation 7 7 8 8 Animals Used 8 Feeds Used 8 Rations Fed to Individual Cows Results Obtained Feed Consumption Milk Production Data Weight Data Comparison of Nutrients Required and Consumed Reproduction Records Feed Cost of Production Bibliography 11 12 12 15 19 19 24 27 29 SUMMARY This bulletin presents the results of long-time feeding trials with second-cutting alfalfa hay for milk pr-oduction. Results are given when alfalfa hay was fed long, when fed chopped, and when fed long with graitt Purebred cows of good productive capacity were used. The average cow in the long-alfalfa-hay group, largely mature animals, weighed 985 pounds; consumed 9,031 pounds of long alfalfa hay, 126 pounds of long oats and vetch hay, and 189 pounds of concentrates; produced 4,421 pounds of milk and 166.2 pounds of butterfat in a 305-day period; and calved again in 13 months. The average cow in the chopped-alfalfa-hay group weighed 1,045 pounds; consumed 8,960 pounds of chopped alfalfa hay, 863 pounds of chopped red clover hay and 76 pounds of concentrates; produced 5,696 pounds of milk and 214.0 pounds of butterfat in a 305-day period; and calved again in 15 months. The average cow in the hay-and-grain group, all immature animals, weighed 888 pounds; consumed 5,664 pounds of long alfalfa hay, 2,060 pounds of ground barley and 1,030 pounds of ground oats; produced 7,208 pounds of milk and 277.9 pounds of butterfat in a 305-day period; and calved again in 14-i months. On the basis of mature equivalent 4 per cent fat corrected milk, the average production was 4,464 pounds with long hay, 5,778 pounds with chopped hay, and 8,416 pounds with long hay and grain. The average production on long hay was therefore only 53 per cent of the average production on hay and grain. The low production on alfalfa hay alone apparently was due to the lack of a sufficient consumption of total digestible nutrients, and probably of phosphorus, to maintain production at a high level. Dairy cows receiving a grain supplement with alfalfa hay were 7 per cent more efficient than cows receiving hay alone in converting feed total digestible nutrients into milk total digestible nutrients. Dairy cows on the average have a capacity for consumption of alfalfa hay alone of about 30 pounds daily per 1,000 pounds of live weight. Dairy cows fed alfalfa hay almost exclusively for as long as five years have reproduced fairly normally. It was economical to chop alfalfa hay at a cost of $2.25 per ton regardless of the value of the hay, if butterfat sold for 30 cents per pound or above. Chopping was not economical with butterfat at 20 cents per pound. It was economical to feed grain with alfalfa hay regardless of the price obtained for the product with concentrates at $20 per ton and alfalfa hay at $5, $10, or $15 per ton. With alfalfa hay at $10 per ton and grain at $30 per ton, it was eco- nomical to feed grain when butterfat was worth 30 cents or more per pound. Experiments now in progress at the Oregon Agricultural Experiment Station are designed to give information on the most economical kind and amount of supplements to feed with alfalfa hay to dairy cows. Studies with Alfalfa Hay for Milk Production By I. R. JONES, P. M. BRANOT, and J. R. HAAG INTRODUCTION ALFALFA became an important crop in the western section of the United States about 1875. Since that time its culture has spread to all parts of this country. In 1930 (1) approximately 38 per cent of all tame hay produced in the United States was alfalfa. In eleven western states 68 per cent of all tame hay produced was alfalfa. In Oregon approximately 30 per cent of the total tonnage of tame hay was alfalfa, the production being approximately 694,000 tons of alfalfa hay. In Eastern Oregon, alfalfa hay constitutes a very large percentage of the tame hay produced. Owing to the relatively low cost of producing alfalfa hay in many of the western irrigated sections, it has become a common practice to restrict dairy cattle more or less entirely to alfalfa. This practice has raised important economic questions and nutritional problems which have been under investigation at the Experiment Station since 1924 (2) (3) (4). This bulletin gives a summary of the more practical results obtained. REVIEW OF LITERATURE It is not the purpose of this bulletin to review the extensive literature comparing the value of alfalfa hay with other hays when fed to dairy cows together with concentrates and succulent feedstuffs. It will suffice to say that alfalfa hay is considered the best hay for dairy cows, followed closely by clovers and other legumes. Feeding alfalfa alone to dairy cattle has been largely a western prac- tice. This is especially true in the irrigated regions of the \Vest where alfalfa culture has reached its highest development. In this section of the country, alfalfa is fed primarily to dairy cattle, but in some sections beef cattle, sheep, and hogs also receive alfalfa as hay, green feed, or pasture as their sole feed throughout the year. In 1918, Woll (5) reported a controlled investigation dealing with the feeding of alfalfa as the sole feed for dairy cattle, He selected two groups of heifers and calves, and to one group fed alfalfa hay and green alfalfa alone and to the other group a mixed ration of alfalfa hay, silage, green corn, and a concentrate mixture. He found about a 20 per cent greater gain in weight with heifers receiving the mixed ration. Insofar as reproduction was concerned, no great difference was noted in the two rations. Six first-lactation records and three second-lactation records were reported for animals in each group. The animals receiving the mixed 5 6 AGRICCLTt-RAL EXPERTMENT STATrON BrLuTrN 328 ration produced on the average 45 per cent more butterfat during the first lactation and 23 per cent more butterfat during the second lactation. The animals on the mixed ration consumed considerably more dry matter and total digestible nutrients. Woll concluded that there was no great difference in the economy of the two systems of feeding. Reed, Fitch, and Cave (6) found that the exclusive feeding of alfalfa hay failed to produce normal growth in Holstein heifers. Alfalfa hay, corn silage, and a grain mixture gave better results. No detrimental effects on reproduction were found with animals fed alfalfa hay as their sole rough- age from about six months of age through two lactation periods. The animals on alfalfa hay alone produced about 4,000 pounds of milk and 150 pounds of butterfat for the first and second lactation periods. A group receiving alfalfa hay and corn silage produced only slightly more. Two groups of animals that received grain in addition to alfalfa hayand corn silage produced on the average about 80 pounds more butterfat for the first lactation and about 100 pounds more butterfat for the second lactation period, as compared to the group on alfalfa hay alone. The authors concluded that with alfalfa hay at $10 per ton and the grain mixture at $30 per ton and with milk and butterfat prices prevailing at the time, it was profitable to feed grain to dairy cattle, the feed cost per pound of butterfat being about 30 when alfalfa hay alone was fed and about 23 per pound when alfalfa hay, corn silage, and grain were fed. Headley, Knight, and Cline (7) report that cows receiving alfalfa hay as the sole ration averaged 7,060 pounds of milk and 263 pounds of butterfat. The results were obtained from a cow-testing association in Nevada for the year 1922-23 and included 702 cows in 53 herds. They found considerable variation in the production of individual cows. Moseley, Stuart, and Graves (8) have reported the milk and butterfat production of 10 cows on three plans of feeding. The ration in one case consisted of alfalfa hay, corn silage, roots, and pasture, and one part of grain mixture for each three pounds of milk produced. During another lactation the same cows were fed similar roughage with one pound of grain mixture to each six pounds of milk produced. In another lactation period the same cows were fed the foregoing roughages but received no grain. All records were made in 365 days under semi-official test conditions including three-times-daily milking. The results are striking in that they show a very high and economical production for cows receiving roughage alone. The results reported on ten cows receiving roughage alone show an average production of 478 pounds of butterfat for the 365 days lactation. The individual animals in this group produced as high as 549 pounds of butterfat on roughage alone. On the limited grain ration the cows produced on the average 584 pounds of butterfat; on the full grain ration they averaged 620 pounds of butterfat. A larger number of animals in the full-grain group were immature animals when the records were made. The animals on the full-grain and limited-grain rations showed a consider- ably greater gain in weight than the animals receiving roughage alone. On the basis of per-ton prices of $10 for alfalfa hay, $32 for grain, $5 for silage, $24 for dried beet pulp, and $6 for beets, the limited grain ration was found to give the highest returns over feed costs, followed by the ration consisting of roughage only, with the full-grain ration being the ALFALFA HAY FOR MILK PRODUCTION / least profitable when butterfat was valued at 44 cents per pound and skim milk at 40 cents per hundred pounds. It was brought out in this study that the animals receiving the roughage ration made considerably better use of the total digestible nutrients supplied than did the animals receiving the full-grain ration. Seven of the ten animals receiving roughage alone as a ration consumed less total digestible nutrients than would be required according to the Savage Feeding Standard. Graves and Shepherd (9) have recently discussed the economics of roughage versus grain feeding for dairy cattle. They report preliminary data on a group of fifteen cows which completed lactations on a ration of alfalfa hay alone as compared to the production of the same animals on a full-grain ration. They report the very high average production of 402 pounds of butterfat and 11,375 pounds of milk by cows on alfalfa hay alone. The average weight of these cows was 1,279 pounds. The same cows, weighing an average of 1,429 pounds, on the full-grain ration produced on the average 18,009 pounds of milk and 620.7 pounds of butterfat. The actual feed supplied to these animals is not given. It is calculated that the average cow receiving alfalfa hay would have to consume almost seven tons of hay, in contrast to five tons of hay and three tons of grain for the cows on a full-grain ration. The authors concluded that a ration of alfalfa hay alone or alfalfa hay and limited grain is more economical than a full-grain ration regardless of the price level at which the product is sold. EXPERIMENTAL OBJECTS OF THE INVESTIGATION This investigation was undertaken with the following objects: To determine the economy of feeding rations of alfalfa hay alone for milk and butterfat production. To compare the feeding value of irrigated and non-irrigated alfalfa hay. To determine the most economical supplements to feed with alfalfa hay. To determine the value of chopping alfalfa hay for dairy cattle. To determine whether cows placed on a ration of alfalfa hay alone would reproduce normally and maintain their health over a period of years. PRELIMINARY INVESTIGATION During the spring of 1924, ten cows were divided into two groups as comparable as possible with respect to breed, age, stage of lactation and gestation, and production. The cows were selected from the regular herd, where they had received a ration of oats-and-vetch hay, corn silage, and a grain mixture consisting of 300 pounds oats, 300 pounds barley, 300 pounds mill run and 100 pounds of protein concentrate. The cows were gradually changed to a ration consisting solely of alfalfa hay, ten days being taken for the transition. Alfalfa hay was fed to the cows in as large 8 AGRICULTURAL EXPERIMENT STATION BULLETIN 328 amounts as they would consume. Accurate records of hay offered and consumed were kept. Two kinds of alfalfa hay were fed, one grown in Central Oregon under irrigation, and the other in the \Villamette Valley, Oregon, without irriga- tion. One group of cows received the Central Oregon hay for 40 days, then after a transition period of 10 days was changed to Willamette Valley hay for 33 days. The second group was started on the Willamette Valley hay and was then changed to the Eastern Oregon hay. The results of this brief trial indicated that the Central Oregon hay was somewhat superior to that grown in the Willamette Valley for maintaining milk production. This was probably due to the greater consumption of the Central Oregon hay, the average daily consumption being 30 pounds of the former and only 24 pounds of the latter. The striking fact brought out in this trial was the rapid decline in milk flow when the cows were changed to the ration of hay alone, the decline for individual cows being from two to seven times faster than normal. MAIN INVESTIGATION Plan of investigation. The results in the preliminary experiment indicated that if definite information was to be obtained the cows must be placed on experiments for one or more lactation periods rather than the customary short-time period of feeding trials. Hence cows were selected from the college dairy herd during dry periods and were fed a ration consisting entirely of alfalfa hay. Animals used. Purebred cows of the four major dairy breeds free from Bang's disease and tuberculosis were used in this investigation. The data on the animals used are given in Table 1. All cows except No. 68 had records in the herd before or after they were used for this investigation. A comparison will be made later in this bulletin of the records made by the cows in the regular herd, contrasted with their production on the experimental ration. For purposes of comparison, the records on the individual cows are given when converted to the basis of 4 per cent fat corrected milk using the Gaines (10) formula and also to a mature equiva- lent 4 per cent fat corrected milk basis using the Graves factor for Ayrshires, the Norton factor for Holsteins, the Copeland factor for Jerseys, the Turner factor for Guernseys, as reported by Graves, Fohrman, and Smith (11). Feeds used. Second-cutting alfalfa hay was used throughout the investigation. The locality where the hay was grown is indicated in Table 2. Since about 130 tons of alfalfa hay were fed in this investigation, the quality of the hay used from year to year varied somewhat, but an attempt was made to purchase hay that contained a good quantity of leaves, had a bright green color, and did not contain a large amount of grass andweeds. The chemical analyses given in Table 2 are of average samples of feed fed throughout the various years and from the particular lots of hay purchased and used during the year. A study of Table 2 indicates that the aver age alfalfa hay fed was somewhat lower in crude protein than that reported by most investigators (12), (13), and (14). This is especially true of the Table 1. RECORDS ON ANIMALS PREVIOUS TO AND AFTER ALFALFA-HAY FEEDING TRIALS 305-day lactation period herd Number and Breed 456 456 623 623 459 457 424 462 468 468 222 228 228 629 511 518 261 68 Ayrshiie Ayrshire Guernsey Guernsey Ayrshire Ayrshire Ayrshire Ayrshire Ayrshire Ayrshire 1-lolstein Holstein Holstein Guernsey Ayrshire Avrshiie Holstein Jersey Year 1924 1-929 1922 1923 1924 1924 1924 1924 1925 1929 1925 1922 1923 1924 1932 1931 1932 Ration Test cow. Regular herd Regular herd Test cow Regular herd Test cow Regular herd Test cow Regular herd Regular herd Regular herd Regular herd Test cow Regular herd Regular herd Regular herd Regular herd Milkings daily 3 Age at calving Calved again Yr.-Mo.-Da 2-3.5 Days Days 383 548 372 432 369 387 481 362 325 624 514 668 481 374 413 352 434 292 417 309 373 324 365 338 310 287 512 419 2 2 79.5 3 3-5-2 2 2.3.17 2-4-2 5 2-3-9 2 6.10-6 2-2-11 2 2-8-5 6-9-2 6-10. 12 2.4-1 4-2-4 3-0-8 2 2 2 2 2 2 2 r Sold as non-breeder. No production record in regular herd. Total days in 6.8-18 5-3-10 5-6-15 milk 601 426 308 370 286 284 Milk Lb. 7780 7793 5341 8622 5535 8768 6421 7253 6369 8960 12434 7748 11357 4498 9141 8530 6644 Butterfat Lb. 306.6 333.0 312.1 523.4 204.3 337.2 223.5 291.7 251.1 387.0 374.4 272.5 426.7 224.6 362.1 346.5 256.0 Mature 4% fat 4% fat corrected corrected milk milk Lb. Lb. 7751 9875 8192 8604 13006 6662 10912 5921 9351 7678 9389 10590 9797 12331 6171 9088 8610 6498 8192 6818 11300 5279 8565 5921 7277 6314 9389 10590 7187 10943 5168 9088 8610 6498 Table 2. AVERAGE CHEMICAL ANALYSts AND OTHER DATA ON FEEDS USED Feedstuff Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Alfalfa, second cutting Clover hay Oats-and-vetch hay Barley Oats 0 Year 1924 1924 1925 1925 1926 1926 t 927 1928 1929 1926 1927 1928 1929 t 928 1929 Where grown -Corvallis, Oregon Redmond, Oregon Corvallis, Oregon Redmond, Oregon Corvallis, Oregon Redmond, Oregon Corvallis, Oregon Hermiston Oregon Corvallis, Oregon Corvallis, Oregon Corvallis, Oregon Corvallis, Oregon Corvallis, Oregon Under irrigation or not No Yes No Yes No Yes No Yes No No No No No Crude protein Crude fiber 12.63 29.70 25.90 30.50 32.90 32.10 30.50 27.60 26.60 28.00 27.83 t2.88 11.56 11.31 9.76 10.28 12.56 12.93 12.3t 8.08 5.94 7.46 8.80 Calcium Phosphorus 27.41 5.20 0.90 0.77 0.03 % 0.22 0.22 0.17 0.12 0.20 0.17 0.17 0.15 0.19 0.17 0.18 0.37 12.60 0.08 0.36 % 1.32 1.15 1.30 1.15 0.98 0.98 1.37 l.t6 1.24 ALFALFA HAY FOR MILK PRODUCTION 11 hay used in 1926. It should also be noted that six out of nine lots of alfalfa hay contained less than 0.20 per cent phosphorus as compared with 0.23 per cent as given by Henry and Morrison (15). Rations led to individual cows. In 1925 a group of eight cows were selected and divided into two groups of four animals each. To one group was fed alfalfa hay grown under irrigation in Central Oregon and to the other group hay grown in the Willamette Valley without irrigation. The first group consisted of animals Nos. 456, 623, 459, and 216. The second group consisted of Nos. 457, 424, 462, and 228. Long alfalfa hay was fed to both groups. Data on two of these cows are not used. Cow No. 216 became lame about the middle of the year and was removed from the experiment. Cow No. 228 received corn silage for a part of the year. Inasmuch as she is one of only two cows that received corn silage as a part of the ration during the investigation, the results will not be reported until additional records are available. In 1926 two animals were added to each group, Nos. 222 and 468 to the group receiving Central Oregon alfalfa and Nos. 228 and 629 to the group receiving Willamette Valley alfalfa. The records on cow No. 424 were not used in 1926 as she became sick after seven months on experiment and was disposed of. Post-mortem examination showed foreign-body infection, a wire being found in the spleen. In 1926 the cows in both groups were fed chopped alfalfa hay. The baled hay was chopped through an ensilage cutter at a cost of $2.25 per ton. Toward the end of the lactation periods a small amount of chopped clover hay was fed. Four of the animals were fed supplements of linseedoil meal or cornstarch for short periods when mineral and nitrogen balance studies were being made as reported in 1929 (12). In 1927 six cows, including four that were in the experiment when it began in 1925 and two that were used in 1926, were continued on the alfalfa- hay experiment. The hay used consisted of lots grown in both Central Oregon and the Willamette Valley and was fed as long hay. Two cows, Nos. 456 and 462, were used in metabolism studies in 1927 (12). In 1928 two of the cows, Nos. 456 and 623, completed their fourth lacta- tion period on a ration consisting entirely of alfalfa hay except for a short period during which No. 456 was used in metabolism studies (13). In 1925 (3), (4), an investigation was begun at the Experiment Station dealing with the mineral requirements for growth, reproduction, and lactation. One of the rations used in this experiment consisted of alfalfa hay and a grain mixture of two parts barley and one part oats. Lactation records are available on four animals for the first lactation and three animals for the second lactation. The production records of this group of animals are used to compare with the production records of the cows receiving rations consisting almost entirely of alfalfa hay. Records obtained. Careful records were kept of all feeds weighed to the individual animals and any refused feeds weighed back. Additional hay was offered in order to obtain maximum consumption. When approximately maximum consumption was determined, however, no large excess of hay was given, otherwise the cows would have eaten the leafy part of the hay and refused the coarser part. 12 AGRICULTURAL EXPERIMENT STATION BULLETIN 328 Milk weights were obtained at each milking and butterfat tests made one day each month. The cows were weighed for three consecutive days at thirty-day intervals. Accurate breeding and reproduction records were kept on all animals used in the investigation. Blood samples for calcium and phosphorus determinations were taken at intervals. Blood tests were made monthly for Bang's disease infection. RESULTS OBTAINED Feed consumption. Table 3 shows the feed consumption data of the cows used in this study. It gives the individual feed consumption data for 305-day periods for 14 cow lactations on long alfalfa hay; 9 cow lactations on chopped alfalfa hay, and 7 cow lactations on long alfalfa hay and a grain mixture consisting of two parts barley and one part oats. A study of Table 3 shows that for the 1925 lactation periods the cows receiving long alfalfa hay as their sole feed, consumed an average of about 9,000 pounds of alfalfa hay for the 305-day lactation period. In 1929, 9 cows received chopped alfalfa hay until practically the end of the lactation period when 5 of the animals were fed clover hay owing to the fact that the supply of alfalfa hay was exhausted. This clover hay was also chopped, hence the total hay consumption for the two years 1925 and 1926 can be more or less directly compared as to the amount of hay that was consumed when fed long and as chopped hay. It will be noted that in 1926 two of the cows received a small amount of additional nutrients in the form of linseed-oil meal and two other cows in the form of cornstarch. This was fed while metabolism studies were being conducted (12). In 1927 and 1928 the experimental cows were again fed long alfalfa hay. In the case of four cows as indicated in Table 3, a small amount of the regular herd mix was fed shortly after calving. A change in plan was then made in order to have available some of these cows for further metabolism studies and the grain mixture was removed from their ration. Nitrogen, calcium, and phosphorus balances were run on animals Nos. 456 and 462 as previously reported (12) (13). The complete lactation record is not available on animal No. 462 for the 1928 season, as she was sold after eight months on the alfalfa hay ration. Oats-and-vetch hay was fed to some of the cows for a few days at the end of the 1927 season, as the supply of alfalfa hay was exhausted. The feed-consumption data for all cows, as indicated in Table 3, are for a 305-day period, the first day's feed record beginning with the first day that milk production data were taken; namely, on the seventh day after calving. Some of the cows did not milk for the total 305-day period and other animals milked considerably longer, but only the records for 305 days are used for comparison. It will be noted in Table 3 that the animals receiving alfalfa hay and grain consumed on the average less than three tons of alfalfa hay and about a ton and a half of grain mixture in contrast to about 4 tons of long hay for the cows receiving this as their main feed and nearly 5 tons for the cows receiving chopped hay. The cows receiving long hay also consumed an average of 189 pounds of concentrates, and the cows receiving chopped hay received an average of 72 pounds of concentrates. Table 3. ToTAL Fzvr, CoNsuMPTION DATA-305-DAY PERIOD Animal number Year Alfalfa Hay, Long CO C0 a CO f1,VV tWV t WV 456 623 459 457 424 462 456 623 459 462 468 222 456 623 Average Alfalfa Hay, Chopped CO a CO CO a CO a CO Wv WV tWV tWV 456 623 459 468 222 457 462 228 629 Average I Alfalfa Alfalfa hay hay consumed fed Lb. I.b. 9t24 .... 9562 9640 9466 9564 9659 9652 9549 8100 8547 9197 9416 11100 9760 8380 9399 1926 1926 1926 1926 1926 1926 1926 1926 1926 .... 9702 8566 9542 8874 9873 9792 9532 10386 7826 9344 9061 10136 7435 8960 1928 1928 1928 1928 1929 1929 1929 5345 5163 5164 6002 5968 6052 6100 5685 5277 5141 5134 5995 5962 6038 6100 5664 1925 1925 1925 1925 1925 1925 1927 1927 1927 1927 1927 1927 1928 1928 8977 8753 8987 9150 9114 9334 7749 8191 8872 9355 10937 9653 8231 9031 9420 8077 8983 8555 9528 9442 Clover hay Oat s-and vetch hay Lb. Lb. Ground barley . .... ... ... ... ... ......- .... . .. . .... .... ... 933 ... 1536 2087 . .. . .... .... .... .... .... .... .. .... ... 1513 1702 863 .... .... ... ... ... ... . . .... .... .... .. Ground corn Lb. Lb. ... ... Cocoanut meal .--. --.. .-.. U... -..- .-.. .. 14 14 7 14 43 28 .... 93 76 38 93 76 38 25 24 19 93 76 38 -... ..-- .... .. .... ...- --.. ---- . ... ... . . ... 5 55 55 19 .. ... .--. ... .... ... .... .... .... .... .... ... .... ... ... ... .... ... ... ... .... ..... ... 1985 1935 2034 1679 2509 2409 1871 2060 993 968 1017 840 1254 1205 935 1030 ... 55 ... .... .. Lb. Lb. .... -... .. 170 .. .... 303 ... .... .... 36 38 .... .... .... .... ... .... -..-.-. ..-. ..-- ---. -.-- -.-. Alfalfa Hay, Long, and Grain 511 68 518 261 511 518 261 Average ... .. .... .... .... ... .... Co indicates alfalfa hay grown under irrigation in Central Oregon. t WV indicates alfalfa hay grown without irrigation in the Willamette Valley, Oregon. . ... .... .... . ... .... .... 170 .... ... .... ... .... .... .... . Corn- starch . .... .. Linseedoil meal .... ... ... . 256 364 390 312 .... 304 ... 135 126 Mill run Lb. Lb. Lb. . ... Ground oats .... ..-. .... ...- .-.... .... --- 38 Table 4. MILK AND BUTTERFAT PRODUcTIoN DATA Animal number Alfalfa Hay, Long "co "CO "CO 456 623 459 tWV 457 ?WV 424 TWV 462 456 623 459 462 468 222 456 623 Average Alfalfa Hat, Chopped "CO "CO "CO "CO "CO 456 623 459 468 222 ?WV 457 9WV 462 SWV 228 tWV 629 Average Alfalfa Hay, Long, and Grain 511 68 518 261 511 518 261 Average Year Age at calving Calved again Da-vs Yr.-Mo.-Da. 1925 1925 1925 1925 1925 1925 1927 1927 1927 1927 1927 1927 1928 1928 1926 1926 1926 1926 1926 1926 1926 1926 1926 1928 1928 1928 1929 1929 1929 1930 Total days in milk 3-3-23 5-7-17 358 250 351 3-3-2 1 326 324 397 314 191 281 242 302 3-4-0 8-1-2 3-2-8 5.7-7 7-7-26 5-5-15 5-5-12 5-0-22 9-4-28 6-5-28 8-8-12 5.9-9 321 381 486 394 615 407 462 339 302 195 328 292 327 400 328 231 391 238 279 5-2.2 1 476 389 457 537 413 338 475 325 635 449 364 310 385 390 364 257 392 289 358 345 3-0-9 413 4-3-16 6-7-3 4-2-13 3-7-0 8-3-11 4-2-22 4-1-23 6-6-6 5.2-6 2-5-2 3 2-5-20 2-6-29 4-1-28 3-9-14 3-5-14 3-1-19 479 320 476 547 352 431 355 (1095)9 368 294 415 354 299 348 Total Milk Total butterfat 305 -day production production lactation milk Lb. 4016 2558 4810 3616 5085 3569 4093 4481 5065 6516 8909 5548 4833 2840 4710 6918 4869 7161 6781 8322 4902 6804 5199 4447 6156 8455 (13059)9 8601 5325 11531 7613 6432 7993 " CO indicates alfalfa hay grown under irrigation in Central Oregon. t WV indicates alfalfa hay grown without irrigation in the Willamette Valley, Oregon. 9 Sold as non-breeder after 3 years in milk; not used in averages. Butterfat 4% fat corrected 305-day milk 305 days lactation Mature equivalent fat corrected milk 305 days Lb. Lb. Lb. Lb. Lb. 148.7 118.0 181.2 135.7 166.0 124.0 144.5 239.5 181.9 224.7 147.3 112.9 179.5 131.1 161.6 124.0 139.7 231.2 178.4 218.3 259.7 159.1 159.5 124.1 166.2 3806 2673 4602 3379 4418 3288 3681 5208 4661 5846 6336 4503 4293 2963 4261 4514 2673 5458 4007 4418 3936 163.6 162.0 130.2 172.2 3990 2448 4772 3530 4984 3569 3962 4350 4963 6427 6100 5291 4750 2753 4421 257.4 242.2 278.1 282.8 280.4 171.0 243.1 161.0 205.2 235.7 6443 4805 6308 6137 7770 4844 5846 5095 4019 5696 234.8 239.3 235.0 255.1 258.8 168.2 196.7 157.2 181.2 214.0 6099 5512 6048 6282 6990 4461 5289 4396 4326 5489 6495 5512 6502 7300 318.2 7831 5580 7714 5325 10182 7393 6432 7208 290.2 279.5 285.2 199.8 369.7 275.8 245.0 277.9 7485 6425 7364 5127 9619 7094 9042 8224 8984 6768 10340 8059 7498 8416 292.0 (785.4)9 321.3 199.8 422.0 286.0 245.0 298.4 6248 7052 3681 5208 4661 5846 6336 4503 4293 2963 4464 6990 4796 5686 4396 4326 5778 15 ALFALFA HAY FOR MJLK PRODUCTION Milk production data. The individual milk and butterfat production data are shown in Table 4. The age at calving, the number of days before the animal calved again, and the total days in milk are given. The total milk and butterfat production of the individual cows is indicated as well as the milk and butterfat production for a lactation period beginning with the seventh day after calving and continuing for 305 days, provided the animal milked that length of time. The 305-day production has been converted to 4 per cent fat corrected milk, using the Gaines formula (10). Also, the 4 per cent fat corrected milk has been converted to the mature equivalent fat corrected milk, based on the age at calving as indicated, by using the age correction factors as previously noted (11). Table 5 gives the average daily milk and butterfat production for the 305-day period, the highest daily milk and butterfat production, and the period of lactation at which maximum production was attained. Table 5. AVERAGE AND MAXIMUM DAILY MILK AND BUTTERFAT PRoDuCTIoN RECORDS305-DAY LACTATION PERIODS Animal number Average daily production Year Alfalfa Hay, Long 5C0 456 CO 623----"CO 459 ?WV 457 tWV tWV 424 462---------- 456 623 459 462 ,,,,, 468 222 456 623 H ilk Butterlat Lb. Lb. 13.1 0.48 0.37 0.59 0.43 0.53 Highest daily milk and butterfat production Day of lactation Amount of milk Day of lactation Lb. Day Lb. Day 15 6 3 1.63 11 1.22 1.65 1.70 0.97 1.79 1.48 1.37 1.63 1.82 0.93 1.25 1.72 1.53 11 Amount 33.3 31.3 31.0 31.7 39.6 28.6 49.1 32.8 40.3 48.0 35.3 31.9 41.8 24.9 35.7 1925 1925 1925 1925 1925 1925 1927 1927 1927 1927 1927 1927 1928 1928 16.3 11.7 13.0 14.3 16.3 21.1 15.6 9.0 14.5 0.46 0.76 0.58 0.72 0.85 0.52 0.52 0.41 0.55 1926 1926 1926 1926 1926 1926 1926 1926 1926 21.1 16.0 20.7 20.1 25.5 15.9 19.2 16.5 13.2 18.7 0.77 0.77 0.77 0.84 0.85 0.55 0.64 0.52 0.59 0.70 36.8 29.1 31.8 40.4 52.2 30.2 33.4 34.7 34.7 1928 1928 1928 1929 1929 1929 1930 25.7 18.3 0.95 0.92 0.94 0.66 36.0 24.8 35.0 Average 8.0 15.6 11.6 20.0 17.3 041 6 21 14 15 16 19 21 20 17 15.5 butterfat 225 A If all a Hay, Chopped CO 456 "CO 623 "CO 459 "CO 468--------- "CO 222 tWV 457 ?WV 462 ?WV 228 ?WV 629 Average Alfalfa Hay. Long, and Grain 511 68 518..... 261 511 518 261 Average 25.3 17.5 33.4 24.2 21.1 23.6 1.21 0.90 0.80 0.91 24.1 28.1 43.9 43.0 32.9 34.8 6 21 14 15 16 19 10 20 10 11.6 1.61 1.31 38 22 28 1.39 1.79 2.56 1.06 1.44 1.27 24 1.01 24.6 1.49 45 21 18 30 61 1.23 1.24 1.14 1.14 1.73 1.44 1.58 1.36 10 45 37 30 8 31 8 38 36 35.6 15 8 31 S 14 24 18.3 81 102 129 17 102 22 9 66.0 " CO indicates alfalfa hay grown under irrgation in Central Oregon. t WV indicates alfalfa hay grown without irrigation in Willamette Valley, Oregon. 16 AGRICULTURAL EXPERIMENT STATION BULLETIN 328 The most striking fact brought out in Tables 4 and 5 is the very iow production of milk and butterfat by good cows receiving alfalfa hay as the main part of their ration. As shown in Table 5, the average cow receiving alfalfa hay attained a maximum daily production of about 35 pounds of milk and 1 pounds of butterfat at about 20 days after calving. The ration of hay alone did not supply the necessary nutrients to maintain this level of production for more than a short period. Ten of the fourteen lactation periods on long hay are for cows that were five years of age or older at the beginning of the lactation period. Four of the records are for cows in their second lactation. These cows, representing three breeds but consisting largely of Ayrshires, produced on the average only 4,421 pounds of milk and 166 pounds of butterfat in a 305-day lactation period. It will be noted that the cows calved again on the average in 391 days, or in about 13 months; so this was not a factor in their low production. It will be noted further that the cows milked onty for an average of about 9 months, the average cow being dry for approximately 4 months. As shown in Tables 4 and 5, the cows receiving chopped alfalfa hay produced considerably better than the cows receiving the long hay. The production of the chopped-hay group wa still very low, however, considering the fact that the cows were largely mature animals of good breeding. The cows used in this group calved again on an average in 15 months and milked an average of 1l months. The cows receiving long alfalfa hay and grain show a considerably higher production of both milk and butterfat. It will be noted that all of the animals in this group were immature, the average age being a little more than three years in contrast to an average age of about five years in the other two groups. The average period between calvings was approximately 14 months. One animal in this group did not have a second calf, and had to be disposed of as a non-breeder after 1,095 days in milk. Hence this figure was not used in computing the average days in milk and the average total production. A summary of the milk and butterfat production of the various groups is given in Table 6. It will be found that there was very little difference in production with alfalfa hay fed long whether grown under irrigation or not. In 1926, when chopped alfalfa hay was fed, the Central Oregon irrigated alfalfa gave about 35 per cent higher production. Reference to Table 5 will show that the group of cows receiving Central Oregon chopped alfalfa hay started the lactation at a higher plane of production than the Willamette Valley hay group. Also, the chopped alfalfa grown in the Willaniette Valley in 1926 was judged the poorest lot of hay used in this investigation. As shown in Table 6, chopped alfalfa hay on the average gave 29 per cent greater production than alfalfa hay fed long. As shown in Table 5 the two groups attained about the same daily maximum production of milk and butterfat, but the chopped hay maintained production at a higher level. It should be noted, however, that the average cow receiving long alfalfa calved again in 13 months in contrast to 14 months between calvings for the cows receiving chopped hay. It is difficult to estimate the effect on production of this failure to breed promptly. Table 6. SUMMARY OP AVERAGE PRODUCTION BY GROUPS YearLong or chopped alfalfa hay Corrected milk Mature equivalent fat corrected milk Lb. Lb. Lb. 3737 6293 146.6 244.6 3694 6186 4215 6560 4028 4951 138.9 175.8 3695 4618 4120 4801 4421 166.2 214.0 277.9 4261 5489 7052 4464 5778 8416 Butterfat production Total butterfat production Milk in milk milk lactation lactation Days Lb. Lb. Lb. 241 363 3795 6810 149.3 268.2 345 443 282 324 4090 5338 141.9 391 279 449 431 4710 345 348 6156 7993 172.2 235.7 289.4 Age at calving Calved Total days Yr.-Mo.-Da. Days 4-1-0 5-4-21 345 454 4-10-31 5-0-8 again Total 305-day 305-day 4% 305 days 305 days Central Oregon (irrigated) Alfalfa i-lay Long Chopped Willantette Ialley (NonIrrigated) Alfalfa Hay Long Chopped Summary All Long All Chopped Long, and Grain 5-9-9 5-2-21 3-1-19 195.1 5696 7208 Table 7. COMPARATIVE PRODUCTION OF 4 PER CENT MILK ON MATURE EQUIVALENT BASIS ON ALSALFA HAY RATIONS AND IN REGULAR HERD IN 305DAY LACTATION PERIODS An Ilnal number 456 623 459 457 424 462 468 222 228 629 SO Total Average Alfalfa hay long Lb. 4514 2673 5458 4007 4418 3936 6336 4503 Alfalfa hay long I Alfalfa hay long Alfalfa hay chopped Total alfalfa hay alone Lb. Lb. Lb. Lb. 3681 5208 4661 4293 2963 6495 18983 16356 16621 5.512 6502 4796 5846 5686 7300 6990 4396 4326 Lactations 4 8803 4418 15468 13636 3 test0 Lb. 9875 13006 8192 8604 6662 10912 t7275 9351 12331 23 Records from Table 1. t Estimated as of semi-officIal test records as no regular herd record was available. Regular herd ration0 Lb. 2 2 11493 4396 4326 114500 4978 SelIliofficial 5921 6234 9389 10590 9797 6171 Estilnated iroduct ion Percentage of regular herd pro. regular herd hay if ill duct ion on alfalfa Lb. 32768 34416 19986 14550 5921 18702 18778 21180 9797 6171 182269 7925 57() 47.5 83.2 60.5 74.6 82.7 72.6 54.3 44.9 69.9 62.8 62.8 ALFALFA HAY FOR MILI PRODUCTION 19 The average production for fourteen cow lactations on long alfalfa hay is shown in Table 6 in comparison to the production on long alfalfa hay and grain. These production results are possibly the most significant in the entire investigation. On the basis of mature equivalent 4 per cent fat corrected milk for a 305-day lactation, the cows receiving hay and grain gave 89 per cent greater production than cows fed largely on long alfalfa hay. Expressed in another way, the cows on long hay gave only 53 per cent of the production of the group receiving hay and grain. Further to point out the capacity for production of the cows used in the alfalfa-hay feeding trials, Table 7 is given. It will be noted that the total production for twenty-three 305-day cow lactations on rations consisting largely of alfalfa hay was 114,500 pounds of 4 per cent milk. Based on their actual records in the regular milking herd, the same cows should have produced 182,269 pounds of 4 per cent milk. On the average lactation basis, this represents a difference between 199 pounds of butterfat on hay alone and 317 pounds of butterfat in the regular herd on a mixed ration, or 118 pounds. Expressed in another way, the cows produced only 63 per cent of the expected production on the rations consisting mainly of alfalfa hay. The group receiving alfalfa hay and grain produced about the same as they later produced in the regular herd. Weight data. The weight data on individual cows used in the investigation are shown in Table 8. The data given include the last weight previous to calving, the first weight after calving, the weight after 305 days lactation, the average weight during the lactation, and the gain in weight. The first weight after calving and the weight previous to calving were not taken at definite periods before or after calving, but averaged about two weeks before calving and about two weeks following calving. A study of Table 5 shows that there was considerable variation of the individual animals in gain in weight during the 305-day period. A study of Table 8 in conjunction with Table 4 indicates that on the average the cows that produced the most milk made the smallest gains in weight. It will also be noted that the average cow receiving the long alfalfa hay was somewhat smaller than the average cow receiving chopped alfalfa hay, but was about 100 pounds heavier than the average animal receiving hay and grain. In other words, in so far as the size of the cow is con- cerned, the cows receiving the chopped hay should have produced the largest amount of milk and butterfat, followed by the cows that received the long alfalfa hay and in turn by the cows that received the alfalfa hay and grain. This difference in weight is largely due to the fact that more immature animals were used in the group receiving alfalfa hay and grain. Comparison of nutrients required and consumed. Table 9 gives a comparison of the calculated digestible crude protein, total digestible nutrients, calcium and phosphorus required and consumed per animal daily in the various groups on the basis of the 305-day lactation period. Table 10 gives a similar comparison based on the feed consumption and maximum production in early lactation as given in Table 5. In determining the digestible crude protein and total digestible nutrients required, the average figures of the Morrison (15) feeding standards for maintenance and milk production have been used. The first weight 20 AGRICULTURAL EXPERIMENT S'rATIoN BULLETIN 328 after calving was used for the maintenance determination. In the case of gain in weight the factor of 4.3 pounds of total digestible nutrients per pound of gain in weight has been applied. This figure was derived from the Armsby (16) figures of 3.25 Therms of net energy for each pound increase in live weight for fattening with no considerable growth and the same figures for growth with no considerable fattening for animals 24 to 30 months of age. Table 8. WeIGHT DATA Animal number Year Alfalfa Hay, Lang 456 623 459 457 424 462 456 623 459 462 468 222 456 623 Average A Ifalf a Hay, Chapped 456 1925 1925 1925 1925 1925 1925 1927 1927 1927 1927 1927 1927 1928 1928 Weight previous to calving weight after calving Lb. Lb. Lb. Lb. 1021 908 853 1062 933 793 657 960 946 786 1070 863 774 937 1002 1219 1093 851 920 1155 971 893 1110 1084 1000 1230 1044 882 775 1035 1015 893 1150 222 908 884 982 1004 1324 886 829 960 45 110 45 2 990 843 745 955 1212 1096 904 850 1029 1413 1177 1043 874 106 798 992 105 74 201 979 1398 1063 1101 936 1318 1058 1045 87 161 10 112 905 690 860 1153 920 910 1233 953 839 666 795 1048 895 863 1110 888 133 48 130 211 1131 934 1249 1004 954 1083 1214 1413 1230 937 1071 First 1171 457 462 228 629 1926 1926 1926 1926 1926 1926 1926 1926 1926 1000 1408 1202 1156 975 892 1237 1053 989 511 68 518 261 511 518 261 1928 1928 1928 1929 1929 1929 1930 1006 725 921 1017 1035 980 1153 977 772 642 730 942 869 815 987 822 623 459 46S 222 Average Alfalfa Hay, Lang, and Grain Average 1009 893 1121 1485 1111 Weight after 305 days 1161 988 1050 Average weight during lactation 1003 1272 1127 920 985 1313 1076 Gain in weight during 305-day experiment Lb. 178 236 150 138 214 160 105 68 137 130 61 202 51 95 246 131 Wellman's (17) mineral standards were used to determine the calcium and phosphorus requirements of the average animal in the various groups. It will be noted that Weilman's standards give a variation in the calcium and phosphorus required for maintenance. Table 9 shows that on the basis of a 305-day lactation and feeding period-the average cow of each group consumed sufficient digestible crude protein, total digestible nutrients, and calcium to meet her requirements. In the case of the phosphorus consumed and required according to the Table 9. COMPARISON OF AVERAGE DIGESTIBLE CRUDE PROTEIN, ToTAL DIGESTIRI.E NUTRIENTS, CALCIUM, AND PIIOSI'IIORUS REQUIRES AND CONSUMED DAILY ON BAsis OF 305-DAY LACTATION Ps;RIOD Average daily requirements Main- Average daily consumption Milk and butterfat tenance Gain in weight production Total Lb. Lb. Lb. Lb. Alfalfa Hay, Long Alfalfa Hay, Chopped Alfalfa Hay, Long, and Grain 0.644 0.692 0.575 0.060 0.83 1 1.07 1 1.535 0.05 1 0.060 1.376 2.011 Total Digestible Nutrients Alfalfa IJay, Long Alfalfa Hay, Chopped Alfalfa 1-lay, Long, and Grain 7.292 7.839 6.518 1.833 1.5 79 1.84 7 4.590 5.911 7.597 15.962 0.0329- 0.0 107 0.05 22 0. 0092 0.06 72 Group Digestible Crude Protein 1.8 14 13.7 15 15. 329 Alfalfa hay Other Daily hay Concen. trateS Total Daily excess deficiency Lb. Lb. Lb. Lb. Lb. Lb. 2.724 2.259 1.783 0.016 0.135 15.282 15.190 9.666 0.193 0.079 0.038 0.62 5 1.4 39 0.141 0.220 7.74 1 2.819 2.432 2.408 15.616 16.849 17.407 1.284 0.6 18 0.397 1.90 1 1.520 1.445 Calcium Alfalfa Hay, Long 0. 065 9 Alfalfa Hay, Chopped 0.03 530.07 08 0.0294- Alfalfa Hay, Long, and Grain 0.09580. 1288 0.1117- 0.36 72 0. 003 2 0.0006 0.37 11 0. 28 79 0. 02 5 5 0. 000 5 0.3 139 0. 004 7 0. 22 7 5 0.2423 0.14 72 0.0 107 0. 08 5 1 0.05 89 0. 1252- 0.2753- 0.2228 0.20220.1667 0.10230.0738 0. 1547 Phosphorus 0.0120- Alfalfa Hay, Long Alfalfa Hay, Long, and Grain 0.0052 0.0290 0.004 5 0. 03 74 0.0052 0. 04 7 3 0.0201 - Alfalfa 1-Jay, Chopped I 0.01290. 02 16 0.01070.0179 0.0462- 0.0489 0. 000 7 0.0042 0.0538 0. 00 7 6 0. 000 5 0.054 3 0.0048 0.0010 0.0601 0. 005 3 0.0034 0.0371 0.0687 0.0055 0. 00 17 0.05 43 0.0548- 0.0635 0.06320.0704 0.03 16 22 AGRICULTURAL EXPERIMENT STATION BULLETIN 328 Wellman standard, there was a small excess or deficiency depending on whether the upper or lower limit of phosphorus required for maintenance as given by Weilnian was used. Table 10 gives much more significant data as to the cause of the low production on hay alone than does Table 9. Table 10 is based on the nutrient requirements and feed consumption in early lactation when the cows were producing at their maximum. The maximum production of the individual cows is given in Table 5. This maximum production in early lactation is considerably less than the maximum production in early lactation of the same cows when being milked in the regular herd. As shown in Table 10 the average cow receiving hay alone, either long or chopped, in early lactation was consuming insufficient total digestible nutrients and phosphorus to meet her requirements. The deficiency amounted to about 30 per cent of the necessary total digestible nutrients and 41 to 45 per cent of phosphorus in the case of the average cow receLving long hay, and about 22 per cent of total digestible nutrients and 34 to 40 per cent of phosphorus in the case of cows receiving chopped hay. While there was also a small deficiency of digestible crude protein there was adequate calcium during the heavy production of early lactation. The cows receiving a grain supplement with alfalfa hay consumed about the required amount of digestible crude protein and total digestible nutrients, but about 20 per cent less phosphorus than is called for by the Wehlman (17) standard, It would seem that low production on hay alone was due to the lack of sufficient total digestible nutrients, and possibly of phosphorus also. What the cows apparently did was to produce a fairly large amount of milk during the first month or two, using nutrients stored in the body, and then to decline rapidly in production to the point where they were able to maintain their bodies and produce a small amount of milk during a short lactation period. During the course of this investigation, a number of samples of blood were taken for the determination of blood-plasma calcium and inorganic phosphorus. Since these results will be discussed in detail in another publication, it is merely desired to point out here that numerous low values were obtained for inorganic phosphorus, a finding which is in harmony with our previously expressed belief (12) (13) to the effect that for more than moderate production, rations restricted largely to alfalfa may often be deficient in phosphorus. Reference to Table 2 shows that most of our lots of alfalfa contained considerably less phosphorus than is commonly accepted as normal. The production of the three groups of cows compared to the normal is shown graphically in the accompanying chart. It will be noted that all groups began lactation at the same level but that the long-alfalfa-hay group declined in production much more rapidly than the group receiving hay and grain or than the normal decline given by Eckles (18). The group receiving chopped hay maintained a higher level of production owing to greater feed consumption than the group receiving long hay. It will be noted that the production curve of the group receiving hay and grain followed quite closely the Eckles curve. Table 10. COMPARISoN OF AVERAGE DIGESTIBLE CRUDE PROTEIN, TOTAL DIGESTIBLE NUTRIENTS, CALCIUM AND PHosplIoRus REQUIRED AND CONLUSIED DAILY ON BASIS OF MAXIMUM PRODUCTION AND FEED CONSUMPTION IN EARLY LACTATION Average daily requirements Group Digestible Crude Protein Alfalfa Hay, Long Alfalfa Hay, Chopped Alfalfa Hay, Long, and Grain Total Digestible Nutrients Alfalfa Hay, Long Alfalfa 1-lay, Chopped Alfalfa Hay, Long, and Grain Average daily consumption Milk and butterfat tenance Gain in weight product ion Lb. Lb. Lb. 0.644 0.692 0.575 0.060 2.178 2.117 2.025 2.882 2.860 2.660 2.54 8 2.386 1.837 0.697 2.534 7.292 7.839 1.833 1.579 12. 29 5 2 1.420 1.84 7 11.951 11.220 21.369 6.5 18 14.293 16.011 9.192 0.741 0.667 8.511 15.034 16.678 17.703 0.03290.0659 0.03530.0 708 0.0294- 0.0107 0.128 5 0.0092 0.1249 0.0 107 0. 1253 Main- 0.05 1 0.060 Total Lb. 18. 585 Alfalfa hay Lb. Other hay Lb. Total Daily excess Lb. Lb. Lb. 0.118 2.666 Collcen. trates Daily deficiency Lb. 0.216 0.474 0.126 2.3 86 6.386 4.69 1 0.882 Calcium Alfalfa Hay, Long Alfalfa I-iay, Chopped Alfalfa 1-lay, Long, and Grain 0.05 89 0.1721- 0.3435 0.0011 0.3446 0.205 1 0.16940.2049 0.1654- 0.1 7250.1395 0.3041 0.3 04 1 0. 13470.1002 0. 204 0 0.0047 0.2087 0.1949 0.0433-. 0.0 138 Phosphorus Alfalfa Hay, Long Alfalfa I-lay, Chopped 0.0120- 0. 00 5 2 .07 14 0. 004 5 .0694 0. 00 5 2 .0696 0. 020 1 0.01 290. 02 16 Alfalfa 1-Jay, Long, and Gi-ain 0.01070.0 179 0.08860. 0967 0.08680. 09 5 5 0.08550. 0927 0.0457 0.0068 0.0574 0. 05 74 0.0289 0.0525 0.0408 0. 069 7 0.03630.0442 0.02940.038 1 0.01580. 023 0 24 AGRICULTURAL EXPERIMENT STATION BULLETIN 328 Forbes and Voris (19) have found that Holstein cows on a mixed ration transformed 20.96 per cent of their feed energy into milk energy during a 313-day lactation period. In our investigation the average transformation of feed total digestible nutrients to milk total digestible nutrients has been calculated from Tables 4 and 9, using 171 pounds of total digestible nutrients in 100 pounds of 4 per cent milk. The average cows on long alfalfa hay transformed 15.30 per cent of the feed total digestible nutrients to milk total digestible nutrients during a 305-day period. The cows on chopped alfalfa hay transformed 18.26 per cent, and those on alfalfa hay and grain 22.71 per cent. It would seem that the feeding of grain with hay resulted in a greater efficiency in the utilization of feed nutrients for milk production. AVERAGE DAILY MILS PRODUCTION WITN THE ADVANCE IN LACTATION Is MONTHS OF LACTATION Reproduction records. The reproduction records of the animals used in this investigation are given in Table 11. A study of this table will show that no great difficulty was experienced in so far as normal reproduction was concerned with the cows receiving long alfalfa hay as the main part of their ration. The group receiving chopped hay did not come in heat as soon and required more services per pregnancy, resulting in a delay in calving to fifteen months after the previous calf. In the case of the group of animals receiving alfalfa hay and grain, considerable difficulty was experienced in getting the animals with calf for their first lactation period. This group of animals received alfalfa hay and grain as their sole ration after weaning from milk through the second and part of the third Table 11. REPRODUCTION RECORDS or ANIMALS ON E.\PERIMENT First Animal number Placed on experiment Ration: Long Alfalfa Mo.-Da.-Yr. I-Jay 456 623 459 457 424 462 Calved Age at calving Mo.-Da.-Yr. Yr.-Mo-Da. 1-21-25 3-3-23 5-7-17 heat period observed Nurnbei of Number serv ices of for con- service Calved bulls ception again P Days 7-7-2 6 61 12 44 41 25 38 50 54 4-12 -2 7 5-5-15 97 4- 28-2 7 222 456 1-24-25 2-1-26 2-1-26 1-24-25 6.30-27 3-26-28 5-5-12 5-0-22 9-4-28 6-5-28 116 45 28 56 623 1-24-25 4-19-28 8-8-12 53 5-9-5 51.4 2.29 46 4 4 456 623 459 462 168 1- 24-2 5 1-24-25 3- 24-25 1-24-2 5 2-18-25 1-30-25 1-24-25 1-24-25 1-24-25 1-24-25 1-24-25 1-24-25 3-15- 25 2-24-25 5-5-27 4-3-21 9- 14-27 3-3-2 1 3-4-0 8-1-2 3-2-8 5-7-7 2 Days Days 358 276 284 calf calf 60 Male Female Male Male 71 63 407 462 277 279 274 271 339 285 52 279.5 Male 391 476 276 288 277 Male Female Female 71 389 457 537 273 Female 65 413 283 Male 97 338 286 Male 69 475 277 Female 63 4 486 394 615 281 284 282 280 275 Condi- tion Reproductive functioning of calf Lb. Female Male I Female Male 321 381 2 of 283 282 3 S 3 Weigh of 65 71 58 91 68 326 324 397 314 2 Sex Female Female Male 351 3 Gestation period Male Male 76 49 60 82 61 Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Normal Uterus and ovaries massaged Normal Normal Normal Normal Weak Normal Normal Yellow body removed I Average Ration: Chopped Alfalfa 1-Jay 456 1-24-25 1- 14-26 4-3-16 3-10-26 1-10-26 6-7-3 459 1-24-2 5 1-24-2 5 468 2-1-26 3-22-26 3-7-0 45 222 2-1-26 5-13-26 8-3-11 130 457 1-24-2 5 12-20-2 5 4-2-22 52 462 1-24 -2 5 1-8-26 4-1-23 91 623 ° Died at three days. t Died day of birth. 4-2-13 16 1.07 180 4 2 1 2 I 66.2 64 63 Normal Normal Normal Nor in al Normal Normal Normal Yellosv bodies removed Retained placenta Normal Yellow bodies removed twice Normal Ovarian cyst and yellow body removed Normal Yellow body removed Weakt Yellow bodies and cysts removed Table II. Animal number PSEPRODUCTION RECORDS OF ANIMALS ON EXPERIMENT (Continued) Age Placed on experiment Calved at calving Mo.-Da.-Yr. I'Io.-Da.-Yr. Yr-Mo-Do. First Number heat of Nunibei period services of obser- for con- service Calved ved ception bulls again Gestation period Days Days Days 325 276 Female 72 Normal Yellosv body Female 55 Retained placenta Normal Yellow bodies 228 11-6-25 3-16-26 6-6-6 57 1 629 2-1-26 6-4-26 5-2-6 110 4 1 635 285 2.44 1.11 449 280.1 413 277 Sex of calf Con di- Neight lion of of call calf Reproductive functioning Lb. removed Average 5.0-7 80.8 68.8 removed I Ration: Lonq Alfalfa Hay and Grain 7-4.25 7-14-28 3-0-9 136 1 1 68 12-22-25 6-10-28 2-5-23 149 27 7 518 1-22-26 7-10-28 2-5-20 395 1 1 261 511 518 7-12-26 7-4-25 1-22-26 2-6-29 8-31-29 11-1-29 2-6-29 4-1-28 3-9-14 21 33 1 1 261 7-12-26 12-23-29 3-5-14 3-1-19 511 Average 284 Male 70 277 282 280 Female 95 59 287 281.1 FemaL 105 3 1 267 t 1 65 1 1.00 352 431 144.3 1.33 61 479 320 476 547 Not pregnant after 27 servIces. Sold as non-breeder; not used in averages. Female Male Male 87 79.5 Normal Retained placenta Ovaries small Yellow bodies removed, cystic ovaries, cervicitis Normal Corpeus luteum and cysts removed Normal Normal Normal Normal Normal Removed cysts three times Normal Normal ALFALFA HAY FOR MILK PRODUCTION 27 lactation period. In contrast to this, the cows that received alfalfa hay as their sole ration were animals from the regular herd that had received a normal ration of hay, grain, and succulent feedstuffs, including pasture, up to and including at least one lactation, and in some cases several lactation periods. The experience in the herd at Oregon State Agricultural College has been that considerably more breeding difficulties are encountered with heifers than with older animals. It will be noted in Table 11 that iii the second pregnancy, with the exception of animal No. 68, which did not calve after twenty-seven services over a period of three years, all the animals calved after one service. It would seem that alfalfa hay alone can be fed with safety to dairy cattle in so far as normal reproduction is concerned, especially after the first calving. Feed cost of production. The economy of grain feeding with a high quality of roughage such as alfalfa hay, depends not only on the comparative milk and butterfat production but also on the comparative prices of hay and grain and the price obtained for the product. In order to illustrate this point, Table 12 has been worked out, using prices of $5, $10, and $15 per ton for long alfalfa hay; $7.25, $12.25, and $17.25 per ton for chopped alfalfa hay; and $20.00, $30.00, and $40.00 per ton for concentrates. The returns over feed cost are given when butterfat sells at prices of 20 cents, 30 cents, 40 cents, and 50 cents per pound. A study of Table 12 shows that when a ration consisting largely of alfalfa hay was fed, it was economical to chop the hay at a cost of $2.25 per ton, regardless of the value of the hay, if butterfat sold for 30 cents per pound or above. With butterfat at 20 cents per pound or less, chopping did not pay. On the basis of results in milk and butterfat production and feed consumption obtained in this investigation, it was economical to feed grain with alfalfa hay, regardless of the price obtained for the product, if concentrates were priced at $20 per ton and alfalfa hay at $5, $10, or $15 per ton. With grain prices of $30 and $40 per ton and alfalfa hay at $5 per ton, grain feeding was econontical if butterfat sold for 40 cents or more per pound. With these feed-price ratios, it was not economical to feed grain when butterfat sold for less than 30 cents per pound. With alfalfa hay at $10 per ton and grain at $30 per ton it was economical to feed grain when butterfat was sold above 30 cents per pound. With $15 alfalfa and $30 grain, it was always economical to feed grain. A survey of Table 12 will show that, on the basis of the milk and butterfat production and feed consumption in this investigation, it is impossible to feed dairy cows with a profit over feed costs when alfalfa hay is worth $10 per ton or more and grain is $30 per ton or more unless butterfat sells for more than 20 cents per pound. In presenting these results it is realized that in actual practice green feeds are used quite extensively. Such feeds increase production. In our investigation no green feeds were used. The influence of green feeds on production will depend to a considerable extent upon the stage of lactation at which they are fed to the cows. Experiments are now in progress which have been designed to give practical information on the most suitable kinds and levels of supplements Table 12. CoSrpAa1ON 01' TIll'. AVERAGE RETURNS OVER FEED COST BASED ON ACTUAL FEED CONSUSIFTION AND MILK PRODUCTION ON THE THREE RATIoNS IN 305-DAY LACTATIONS WITH VARYING HAY, CONCENTRATE, AND BUTTERFAT PRICES Long hay $5.00 per ton. chopped hay $7.25 per toll Butterfat Price of coilcentrates pel ton-Ration 50 Butter. fat 40 Butterfat 30 Long hay $10.00 per ton. Chopped hay $12.25 per ton Butterfat 20 Butterfat 5O Butterfat 40 Butterfat 30 Butterfat 20 Long hay $15.00 per ton. Chopped hay $17.25 per ton. Butterfat 50 Butterfat 40 I Butterfat 30 Butterfat 20 pOulId pOulId per per pound per pound per pound per pound per pound per pound per pound per pound per pound per pound $59.90 72.28 $42.86 50.32 $25.81 28.37 $ 8.77 $37.54 48.85 $20.50 26.89 $ 3.45 $-13.59 $10.79 6.41 4.94 23J7 $-6.25 17M2 1.21 $-23.30 -20.74 $-40.34 -42.70 95.98 67.77 39.56 11.36 81.82 53.61 25.40 -2.80 67.66 39.45 11.24 -16.96 59.52 71.33 42.48 49.47 25.43 27.42 8.39 5.46 36.60 48.47 19.56 26.51 2.51 4.56 -14.53 10.41 -6.63 0.26 -23.68 -21.69 -40.72 -43.65 80.53 52.32 24.11 -.4.09 66.37 38.16 9.95 -18.25 52.21 24.00 -3.21 -32.41 59.14 70.39 42.10 48.53 25.05 26.48 8.01 4.52 35.65 48.09 18.61 26.13 2.56 4.18 -15.48 -17.78 10.03 21.28 -7.01 -0.68 -24.06 -4110 65.08 36.87 8.66 -19.54 50.92 22.71 -5.50 -33.70 36.76 8.55 -19. 66 per $20.00 Alfalfa Hay, Long Alfalfa Hay, Chopped. Alfalfa Hay, Long, and Grain $30.00 Alfalfa hay, Long Alfalfa Flay, Chopped Alfalfa Flay, Long, and Grain -t7.40 22.22 $40.00 Alfalfa Hay, Long Alfalfa Hay, Chopped Alfalfa Hay, Long, and Grain -2 2.63 -44.60 -47.86 ALFALFA HAY FOR MILK PRODUCTION 29 to be used in various sections when dairy cattle are restricted largely to alfalfa hay. It is our belief that the ultimate solution of this problem will involve well-chosen supplements during the early lactation period. It is during the first three or four months of the lactation period that the economy of milk production for the entire cycle is particularly influenced by supplemental feeding. BIBLIOGRAPHY Yearbook of Agriculture, 1931, United States Department of Agriculture, pp. 804-808. 1931. Director's Biennial Report, 1924-26, Oregon Agricultural Experiment Station, pp. 41-43. Director's Biennial Report, 1926-28, Oi-egon Agricultural Experiment Station, pp. 53-54. Director's Biennial Report, 1920-30, Oregon Agricultural Experiment Station, pp. 83-84. Il/oil, F. W. Alfalfa as a Sole Feed for Dairy Cattle. Jour. Dairy Sci. V. I, pp. 447-461. 1918. Reed, 0. E., Fitch, J. B., and Care, H. W. The Relation of Feeding and Age of Calving to the development of Dairy Heifers. Kansas Agr. Experiment station. Bul. 233, 38 p. 1924. Headley, F. B., Knight, E. W., and Cline, L. E. Work of the Newlands Reclamation Project Experiment Farm in 1922 and 1923. U. S. Department of Agriculture Cir. 352, 27 p. 1925. Moseley, T. W., Stuart, Duncan, and Graves, R. R. Dairy Work at the Huntley Field Station, Huntley, Montana. 1918-1927. U. S. Department of Agr. Tech. Bulletin 116, pp. 15-22. 1929. Graves, R. R., and Shepherd, J. B. A Study of Certain Phases of the Economics of Dairy-Cattle Feeding. Bureau of Dairy Industry. U. S. Department of Agr. Roughage Feeding Series Mimeograph, 26 p. 1933. Gaines, W. L. The Energy Basis of Measuring Milk Yield in Dairy Cows. Ill. Agr. Experiment Station. Bulletin 308. May 1928. Graves, R. R., Fohrman, M. H., and Smith, R. H. A Study of Age-Correction Factors. Bureau of Dairy Industry, U. S. Dept. of Agr. Mimeograph, 21 p. 1933. Haag, J. R., Jones, J. S., Jones, I. R., and Brandt, P. M. The Physiological Effect of Rations Restricted Principally or Solely to the Alfalfa Plant, I. The Calcium, Phosphorus and Nitrogen Metabolism of Dairy Cattle. Jour. Dairy Sci. V. 12, pp. 445-455. 1929. Haag, J. R., Jones, I. R., and Braudt, P. M. The Physiological Effect of Rations Restricted Principally or Solely to the Alfalfa Plant III. The Influence of Various Mineral Supplements on the Calcium, Phosphorus, and Nitrogen Metabolism of Dairy Cattle. Jour. Dairy Sci. V. 15, pp. 23-28. 1932. 30 AGRJCI'LTI'RAL EXPERIMENT STATION BI'LLETIN 328 Huff juan, C. F., Duncan, C. W., Robinson, C. S., and Lamb, L. W. Phos- phorus Requirements of Dairy Cattle When Alfalfa Furnishes the Principal Source of Protein. Michigan Agr. Experiment Station Tech. Bulletin 134, 75 p. 1933. Henry, W. A., and Morrison, F. B. Feeds and Feeding. Henry-Morrison Company, Madison, Wisconsin. 18th Edition, pp. 746-747. 1923. Armsbv, H. P. The Nutrition of Farm Animals. The MacMillan Co., New York, p. 712. 1917. WelInian, 0. Futterungsversuche uber die Bedeutung des Mineraistoffwechsels, inbesondere der Erdalkalien und Phosphorsaure. Biologia Generalis, Vol. 8, pp. 387-396. 1932. Eckles, C. H. Dairy Cattle and Milk Production. The MacMillan Company. Rev. Edition, p. 410. 1923. Forbes, E. B., and lions, LeRoy. The Economy of Conversion of Food Energy into Milk Energy by the Dairy Cow. Journal of Nutrition, \o1. 5, pp. 395-401. 1932. flPlC.fl?sI STATT RflAP1) ()1? WT(T-TTlR 1OUCATION C. Samrnons Lief S. Finseth B. F. Irvine \Villard L. Marks Herman Oliver Cornelia Marvin Pierce E. Callister B. McLeod C. A. Brand \V. J. Kerr, D.Sc., LL.D i-'ortland Dallas Portland Albany Canyon City La Grande Albany Portland Roseburg Chancellor of Higher Education STAFF OF AGRICULTURAL EXPERIMENT STATION Staff members marked are United States Department of Agriculture investigators stationed in Oregon President of the State College Geo. W. Peavy, M.S.F Director Wm. A. Schoenfeld, B.S.A., M.B.A Vice Director R. S. Besse, M.S Division of Agricultural Economics Agricultural Economist; In Charge, Division of Agricultural E. L. Potter, M.S Economics Agricultural Economics Agricultural Economist W. H. Dreesen, Ph.D Farm Management Economist (Farm Management) D. Scudder, B.S Associate Economist (Farm Management) H. E. Selby, M.S Associate Economist (Farm Management) G. W. Kuhlman, M.S Associate Economist (Farm Management) S. Burner, M.S._ Division of Animal Industries P. H. Brandt, A.M Dairy Husbandman; In Charge, Division of Animal Industries Animal Husbandry Animal Husbandman 0. H. Nelson, M.S Assistant Animal Husbandinan W. Rodenwold, B.S Assistant Animal Husbandman \V. Oliver, M.S Dairy Husbandry - Gustav Wilster, Ph.D Dairy Husbaridman (Dairy Manufacturing) Associate Dairy Husbandman I. R. Jones, Ph.D Poultry Husbandry Poultry Husbandiiiaii A. G. Lunn, B.S Poultry Husbandman F. L. Knowlton, M.S Associate Poultry Husbandman F. K. Fox, M.S Veterinary Medicine Veterinarian T. Simms, D.V.M Poultry Pathologist W. T. Johnson, B.S., D.V.M Associate Veterinarian J. N. Shaw, B.S., D.V.M Associate Veterinarian, Bureau of Animal Industries R. Jay, D.V.M Assistant Poultry Pathologist E. H. Dickinson, D.V.M Associate Veterinanian F. M. Bolin, D.V.M Junior Veterinarian" 0. H. Muth, D.V.M Technician 0. L. Searcy, B.S Division of Plant Industries Agronomist; In Charge, Division of Plant Industries G. R. Hyslop, B.S Farm Crops D. D. Hill, M.S Associate Agronomist H. A. Schoth, M.S...Associate Agronomist; Forage Crops and Disease Investigation D. C. Smith, Ph.D Assistant Agronomist B. B. Robinson, Ph.D Assistant Plant Breeder, Fiber Flax Investigations" Grace Cole Fleischman, A.B Assistant Botanist, Division of Seed Investigations" Horticulture W. S. Brown, D.Sc Horticulturist A. G. B. Bouquet, M.S Horticultucis (Vegetable Crops) E. H. Wiegand, B.S.A Horticulturist (Horticultural Products) Horticulturist (Pomology) H. Hartman, M.S C. E. Schuster, 1\{.S Horticulturist (Nut Culture) Horticulturist (Plant Propagation) W. P. Duruz, Ph.D G. F. Waldo, M.S Assistant Pomologist (Small Fruit Investigations)5 J. C. Moore, M.S Assistant Horticulturist (Pomology) Assistant Horticulturist (Horticultural Products) T. Onsdortf, B.S STATION STAFF(Continsied) Soil Science W. L. Powers, Ph.D Soil Scientist C. V. Ruzek, M.S Soil Scientist (Fertility) M. -R. Lewis C.E Irrigation and Drainage Engineer, Bur. of Agric. Engineering R. E. Stephenson, Ph.D Associate Soil Scientist E. F. Torgerson, B.S Assistant Soil Scientist( Soil Survey) J. S. Jones, M.S.A R. H. Robinson, M.S J. R. Haag, Ph.D D. E. Bullis, M.S H. B. Hatch, B.S F. E. Price, B.S C. Ivan Branton, B.S G. V. Copson, M.S J. E. Simmons, M.S Other Departments Agricultural Cliemiitry Chemist in Cliaige Chemist (Insecticides and Fungicides) Chemist (Animal Nutrition) .Associate Chemist (Horticultural Products) Assistant Chemist Agricultural Engineering Agricultural Engineer Assistant Agricultural Engineer Bacteriology W. B. Bollen, Ph.D D. B. Charlton, Ph.D D. C. Mote, Ph.D A. 0. Larson, M.S B. G. Thompson, M.S F G. Hinman., M.S E. Dimick, M.S C. Jones, M.S K. W. Gray, B.S W. ID. Edwards, B.S Hand H. Wilson, A.M C. E. Owens, Ph.D S. M. Zeller, Ph.D B. F. Dana. M.S F. P. McWlsorter, Ph.D D. Bailey, M.S W. Miller, Ph.D R. Hoerner, M.S T. Dykstra, M.S Roderick Sprague, Ph.D Bacteriologist in Charge Associate Bacteriologist Associate Bacteriologist Assistant Bacteriologist Entomology Entomologist in Charge Entomologist (Stored Products Insects)" Assistant Entomologist Junior Entomologist (Stored Products Insects)" Assistant Entomologist Assistant Entomologist Field Assistant (Entomology) Field Assistant (Entomology) Home Economics Plant Pathology Home Economist Plant Pathologist Plant Pathologist Pathologist (Curley Top Diseases of Vegetables)" Plant Pathologist Associate Pathologist (Enforcement of Insecticide Act) * Associate Pathologist (Nut Disease Investigations)" Agent (Hop Disease Investigations)" Assistant Plant Pathologist (Potato Diseases)" Assistant Pathologist (Cereal Diseases)z H. H. Millsap Agent (Bulb Diseases)" Publication5 and News Service C. D. Byrne, M.S Director of Information Editor of Publications E. T. Reed, B.S., A.B D. M. Goode, B.A Associate Editor of Publications Associate in News Service J. C. Burtner, B.S Branch Stations D. E. Stephens, B.S Supt., Sherman Br. Expt. Sta., Moro; Sr. Agronomist" L. Childs, A.B Superintendent, Hood River Br. Expt. Station, Hood River Superintendent, Southern Oregon Br. Expt. Station, Talent F. C. Reimer, M.S D. E. Richards, B.S..........Superintendent, Livestock Br. Experiment Station, Union Superintendent, Umatilla Br. Experiment Station, Hermiston" H. K. Dean, B.S Superintendent, Harney Valley Br. Experiment Station, Burns 0. Shattuck, M.S Superintendent, John Jacob Astor Br. Expt. Sta., Astoria A. E. Engbretson, B.S Acting Superintendent, Pendleton Field Station, Pendleton; G. A. Mitchell, B.S Assistant Agronomist, Division of Dry Land Agriculture" Arch Work, B.S Acting Supt. Medford Br. Expt. Sta., Medford; Associate Irrigation Engineer, Bureau of Agricultural Engineering" W. W. Aldrich, Ph.D.Assistant Horticulturist, Bureau of Plant Industry, Medford" G. G. Brown, A.B., B.S Horticulturist, Hood River Br. Expt. Station, Hood River Associate Entomologist, Sou. Or. Br. Expt. Sta., Talent L. G. Geniner, It{.S J. F. Martin, M.S Junior A?r000mist, Div. Cereal Crops and Diseases, Pendleton" Assistant to Supt., Sherman Br. Experiment Station, Moro H. H. Oveson, M.S R. B. Webb, M.S Jr. Agronomist, Sherman Branch Experiment Station, Moro R. E. Hutchinsomi, B.S Asst. to Supt., Harney Valley Br. Expt. Sta., Burns