MEAT
TECHSPEC REVIEW
Ryan Alexander
Janice O'Brien
Anonymous student CE
20.020
April 8,2008
4/6/09
Impact
Use of cattle as source of meat is highly resource
demanding
zMethane gas emitted from cattle contributes to
global warming
zEthical issues with treatment of animals
zPresence of high levels of synthetic hormones and
antibiotics in present-day meat
zRisk of contamination during development or
slaughter
z
4/6/09
Purpose
Develop a procedure with tools from tissue
engineering that allow for the construction of
edible tissue that closely resembles the presentday meat product and addresses nutritional and
safety concerns
z
4/6/09
3 Step Process
1) Differentiate/Detach Differentiated
Muscle Cell
Myoblast
Differentiation
Stent
Skin cell line 1
2) Attach
Skin cell line 2
3)Exercise
4/6/09
Stent
Exercise
FINAL PRODUCT
4/6/09
System and Device Overview
4/6/09
Differentiate/Detach
Muscle Cell
Skin Cells
Growth
Factors
Muscle Precursor
Cell
Differentiate/
Repress
Adhesion
Divide
lacl repressor
Muscle Precursor
Cell
4/6/09
Differentiate/Detach
Skin Cell (cell line1)
Inhibition
Growth
Factor
Receptor
lacl
Lacl
secreter
Division
Differentiation
Adhesion
Molecule1
Repressor
Producer
Muscle Precursor Cell
4/6/09
GF
Adhesion
Molecule1
Inhibition
GF
lacl
Growt
h
Factor
Secret
er
Differentiate/Detach
GF
Secreter
GF
Receptor
Differentiation
Division
Lacl secreter
Adhesion
Molecule1
Producer
TIME
4/6/09
Attach
Skin Cells on
Collagen
Scaffold
4/6/09
Adhesion
Protein
Muscle Cells
Cell
Adhesion
Attach
Skin Cell (cell line 2)
Adhesion
Molecule 2
Producer
Activator
Activator
Secreter
Muscle Cell
Adhesion
Molecule 2
Producer
4/6/09
Activator
Attach
Adhesion
Molecule2
Producer
Activator
Secreter
Adhesion
Molecule2
Producer
TIME
4/6/09
Exercise
Exercise
Factors
Skin Cellss
4/6/09
Muscle Cellss
Gain in
Muscle
Tone
Exercise
Skin Cells(Cell Line 2)
lacl
IGF-1
Inhibition
IGF-1
Producer
Muscle
Tone
IGF-1
receptor
Lacl
secreter
4/6/09
IGF-1
lacl
Exercise
IGF-1
Producer
IGF-1
Receptor
LacI
secreter
4/6/09
PARTS
4/6/09
Differentiate/Detach
Growth Factor Secreter
Promoter LacI operon
Lacl secreter
Promoter
RBS
RBS
/
MyoD Protein Sequence
Adhesion Molecule1 Repressor Producer
Differentiation LacI Protein
Sequence
Sequence
BBa_C0012
Promoter
4/6/09
Cl binding
site
RBS
CDH-15 Protein
Sequence
Cl Repressor Protein
Sequence
CI HK022
Attach
Adhesion Molecule 2 Producer
Promoter
Promoter
RBS
NCAM Protein
Adhesion
Molecule2
Protein Sequence
Sequence
Adhesion Molecule 2 Producer Version 2
appY
binding site
RBS
NCAM Protein
Sequence
Activator Secreter
Promoter
RBS
AppY protein sequence
BBa_K118001
4/6/09
Version 1
Exercise
IGF-1 Producer
Promoter
LacI binding
site
RBS
IGF-1 Protein Sequence
Lacl secreter
Promoter
RBS
Hypertrophy Gene
LacI Protein
Sequence
BBa_C0012
4/6/09
Testing/Debugging
z
z
z
Differentiate/Detach: Removal of differentiating system and comparison of before/after muscle cell concentration
Attach: Attachment of muscle cells to collagen scaffold indicates system working
Exercise: Compare to non‐stimulated muscle cells
4/6/09
Unknowns and Possible Issues
•
Yield of procedure?
•
Percentage of floating muscle cells harvested?
4/6/09
Open Issues
•
Bioreactor or not?
•
Different pathways?
4/6/09
Go or No Go?
4/6/09
MIT OpenCourseWare
http://ocw.mit.edu
20.020 Introduction to Biological Engineering Design
Spring 2009
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